GB2333154A - Assay for duroc muscle fibre tyre - Google Patents

Assay for duroc muscle fibre tyre

Info

Publication number
GB2333154A
GB2333154A GB9907504A GB9907504A GB2333154A GB 2333154 A GB2333154 A GB 2333154A GB 9907504 A GB9907504 A GB 9907504A GB 9907504 A GB9907504 A GB 9907504A GB 2333154 A GB2333154 A GB 2333154A
Authority
GB
United Kingdom
Prior art keywords
muscle
assay
duroc
sample
present per
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9907504A
Other versions
GB2333154B (en
GB2333154A8 (en
GB9907504D0 (en
Inventor
Charlotte Anne Maltin
John Steven
Christopher Charles Warkup
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Meat and Livestock Commission
Original Assignee
Meat and Livestock Commission
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9620904.4A external-priority patent/GB9620904D0/en
Priority claimed from GBGB9703350.0A external-priority patent/GB9703350D0/en
Priority claimed from GBGB9705796.2A external-priority patent/GB9705796D0/en
Priority claimed from GBGB9719002.9A external-priority patent/GB9719002D0/en
Priority claimed from PCT/GB1997/002741 external-priority patent/WO1998015837A1/en
Priority to GB9907504A priority Critical patent/GB2333154B/en
Application filed by Meat and Livestock Commission filed Critical Meat and Livestock Commission
Publication of GB9907504D0 publication Critical patent/GB9907504D0/en
Publication of GB2333154A publication Critical patent/GB2333154A/en
Publication of GB2333154A8 publication Critical patent/GB2333154A8/en
Publication of GB2333154B publication Critical patent/GB2333154B/en
Application granted granted Critical
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6887—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from muscle, cartilage or connective tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

There is provided an assay to determine whether an animal has an allele for or exhibits muscle fibre composition characteristic of a Duroc pig. The assay comprises obtaining a tissue sample from said animal and subjecting said sample to genetic analysis and/or obtaining a muscle sample from said animal and determining by histochemical or immunochemical analysis whether said sample exhibits phenotypic traits indicative of said allele(s) and/or shows a muscle composition characterisitc to the Duroc pig. Suitable physical traits include the following: i. the percentage frequency of SO fibres present in said muscle; and/or ii. the number of SO fibres present per cluster; and/or iii. the number of muscle fibres present per cluster; and/or iv. the level of m calpain present per unit muscle; and/or v. the level of Á calpain present per unit muscle. The assay can be used to select animals having Duroc muscle type for breeding or for meat production. Anti-calpain antibodies are especially useful and may be employed as part of an ELISA style assay.
GB9907504A 1996-10-07 1997-10-07 Assay for duroc muscle fibre tyre Expired - Fee Related GB2333154B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9907504A GB2333154B (en) 1996-10-07 1997-10-07 Assay for duroc muscle fibre tyre

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GBGB9620904.4A GB9620904D0 (en) 1996-10-07 1996-10-07 Chemical compound
GBGB9703350.0A GB9703350D0 (en) 1997-02-18 1997-02-18 Chemical compound
GBGB9705796.2A GB9705796D0 (en) 1997-03-20 1997-03-20 Chemical compound
GBGB9719002.9A GB9719002D0 (en) 1997-09-09 1997-09-09 Chemical compound
PCT/GB1997/002741 WO1998015837A1 (en) 1996-10-07 1997-10-07 Assay for duroc muscle fibre type
GB9907504A GB2333154B (en) 1996-10-07 1997-10-07 Assay for duroc muscle fibre tyre

Publications (4)

Publication Number Publication Date
GB9907504D0 GB9907504D0 (en) 1999-05-26
GB2333154A true GB2333154A (en) 1999-07-14
GB2333154A8 GB2333154A8 (en) 1999-07-19
GB2333154B GB2333154B (en) 2001-05-09

Family

ID=27517375

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9907504A Expired - Fee Related GB2333154B (en) 1996-10-07 1997-10-07 Assay for duroc muscle fibre tyre

Country Status (1)

Country Link
GB (1) GB2333154B (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011387A1 (en) * 1990-12-21 1992-07-09 The University Of Toronto Innovations Foundation Diagnosis for porcine malignant hyperthermia
WO1997035878A2 (en) * 1996-03-28 1997-10-02 Pig Genes B.V. I.O. The porcine heart fatty acid-binding protein encoding gene and methods to identify polymorphisms associated with body weight

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011387A1 (en) * 1990-12-21 1992-07-09 The University Of Toronto Innovations Foundation Diagnosis for porcine malignant hyperthermia
WO1997035878A2 (en) * 1996-03-28 1997-10-02 Pig Genes B.V. I.O. The porcine heart fatty acid-binding protein encoding gene and methods to identify polymorphisms associated with body weight

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Lab. Animal Sci. Vol. 44 (1) 1994. Kretchmar D H et.al. pages 38-41 *
Zivocisna Vyroba Vol. 31 (12) 1986. Uhrin V et.al. pages 1065-74;Bio. Abs. Vol. 83 1986 No. 93226 *

Also Published As

Publication number Publication date
GB2333154B (en) 2001-05-09
GB2333154A8 (en) 1999-07-19
GB9907504D0 (en) 1999-05-26

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20061007