GB2331751A8 - Quiescent cell populations for nuclear transfer - Google Patents

Quiescent cell populations for nuclear transfer

Info

Publication number
GB2331751A8
GB2331751A8 GB9824304A GB9824304A GB2331751A8 GB 2331751 A8 GB2331751 A8 GB 2331751A8 GB 9824304 A GB9824304 A GB 9824304A GB 9824304 A GB9824304 A GB 9824304A GB 2331751 A8 GB2331751 A8 GB 2331751A8
Authority
GB
United Kingdom
Prior art keywords
nuclear transfer
quiescent
cell populations
cell
quiescent cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9824304A
Other versions
GB2331751A (en
GB9824304D0 (en
GB2331751B (en
Inventor
Ian Wilmut
Keith Henry Stockman Campbell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Minister of Agriculture Fisheries and Food UK
Biotechnology and Biological Sciences Research Council
Roslin Institute
Original Assignee
Minister of Agriculture Fisheries and Food UK
Biotechnology and Biological Sciences Research Council
Roslin Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9517780.4A external-priority patent/GB9517780D0/en
Application filed by Minister of Agriculture Fisheries and Food UK, Biotechnology and Biological Sciences Research Council, Roslin Institute filed Critical Minister of Agriculture Fisheries and Food UK
Priority to GB9824304A priority Critical patent/GB2331751B/en
Publication of GB9824304D0 publication Critical patent/GB9824304D0/en
Publication of GB2331751A publication Critical patent/GB2331751A/en
Publication of GB2331751A8 publication Critical patent/GB2331751A8/en
Application granted granted Critical
Publication of GB2331751B publication Critical patent/GB2331751B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8773—Ovine embryos

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Gynecology & Obstetrics (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)

Abstract

A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the GO state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more animals. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
GB9824304A 1995-08-31 1996-08-30 Quiescent cell populations for nuclear transfer Expired - Lifetime GB2331751B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9824304A GB2331751B (en) 1995-08-31 1996-08-30 Quiescent cell populations for nuclear transfer

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9517780.4A GB9517780D0 (en) 1995-08-31 1995-08-31 Biological manipulation
GB9824304A GB2331751B (en) 1995-08-31 1996-08-30 Quiescent cell populations for nuclear transfer
GB9802915A GB2318578B (en) 1995-08-31 1996-08-30 Quiescent cell populations for nuclear transfer

Publications (4)

Publication Number Publication Date
GB9824304D0 GB9824304D0 (en) 1998-12-30
GB2331751A GB2331751A (en) 1999-06-02
GB2331751A8 true GB2331751A8 (en) 1999-06-03
GB2331751B GB2331751B (en) 2000-01-19

Family

ID=26307658

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9824304A Expired - Lifetime GB2331751B (en) 1995-08-31 1996-08-30 Quiescent cell populations for nuclear transfer

Country Status (1)

Country Link
GB (1) GB2331751B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
NZ524523A (en) 2000-08-03 2006-02-24 Therapeutic Human Polyclonals Production of humanized antibodies in transgenic animals
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
AU2019247490A1 (en) 2018-04-06 2020-10-22 Children's Medical Center Corporation Compositions and methods for somatic cell reprogramming and modulating imprinting

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2092258A1 (en) * 1992-12-30 1994-07-01 Steven L. Stice Synchronized donor cells for bovine nuclear transfer
GB9417831D0 (en) * 1994-09-05 1994-10-26 Biotech & Biolog Scien Res Biological manipulation

Also Published As

Publication number Publication date
GB2331751A (en) 1999-06-02
GB9824304D0 (en) 1998-12-30
GB2331751B (en) 2000-01-19

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PE20 Patent expired after termination of 20 years

Expiry date: 20160829