GB2325232A - Plants having enhanced nitrogen assimilation/metabolism - Google Patents

Plants having enhanced nitrogen assimilation/metabolism

Info

Publication number
GB2325232A
GB2325232A GB9817804A GB9817804A GB2325232A GB 2325232 A GB2325232 A GB 2325232A GB 9817804 A GB9817804 A GB 9817804A GB 9817804 A GB9817804 A GB 9817804A GB 2325232 A GB2325232 A GB 2325232A
Authority
GB
United Kingdom
Prior art keywords
metabolism
plants
promoter
nitrogen assimilation
nitrogen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9817804A
Other versions
GB9817804D0 (en
GB2325232B (en
Inventor
Allen G Good
Virginia L Stroeher
Douglas G Muench
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Alberta
Original Assignee
University of Alberta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA2169502A external-priority patent/CA2169502C/en
Priority claimed from US08/599,968 external-priority patent/US6084153A/en
Application filed by University of Alberta filed Critical University of Alberta
Priority to GB0023359A priority Critical patent/GB2349886B/en
Publication of GB9817804D0 publication Critical patent/GB9817804D0/en
Publication of GB2325232A publication Critical patent/GB2325232A/en
Application granted granted Critical
Publication of GB2325232B publication Critical patent/GB2325232B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fertilizers (AREA)
  • Hydroponics (AREA)

Abstract

A genetic construct is taught which contains a nitrogen assimilation and/or metabolism enzyme coding sequence operably associated with an inducible promoter. Preferably, the promoter is inducible under conditions where it would be beneficial to take-up, store or use nitrogen. The promoter can be, for example, induced by the presence of a selected chemical agent, such as nitrate or other form of nitrogen, preferably by a nitrogenous fertilizer.
GB9817804A 1996-02-14 1997-02-14 Plants having enhanced nitrogen assimilation/metabolism Expired - Lifetime GB2325232B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB0023359A GB2349886B (en) 1996-02-14 1997-02-14 Plants having enhanced nitrogen assimilation/metabolism

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CA2169502A CA2169502C (en) 1996-02-14 1996-02-14 Plants having enhanced nitrogen assimilation/metabolism
US08/599,968 US6084153A (en) 1996-02-14 1996-02-14 Plants having enhanced nitrogen assimilation/metabolism
PCT/CA1997/000100 WO1997030163A1 (en) 1996-02-14 1997-02-14 Plants having enhanced nitrogen assimilation/metabolism

Publications (3)

Publication Number Publication Date
GB9817804D0 GB9817804D0 (en) 1998-10-14
GB2325232A true GB2325232A (en) 1998-11-18
GB2325232B GB2325232B (en) 2000-11-29

Family

ID=25678337

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9817804A Expired - Lifetime GB2325232B (en) 1996-02-14 1997-02-14 Plants having enhanced nitrogen assimilation/metabolism

Country Status (3)

Country Link
AU (1) AU727264B2 (en)
GB (1) GB2325232B (en)
WO (1) WO1997030163A1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7390937B2 (en) 1996-02-14 2008-06-24 The Governors Of The University Of Alberta Plants with enhanced levels of nitrogen utilization proteins in their root epidermis and uses thereof
US6084153A (en) 1996-02-14 2000-07-04 The Governors Of The University Of Alberta Plants having enhanced nitrogen assimilation/metabolism
HUP0203693A2 (en) * 1999-08-26 2003-04-28 Basf Plant Science Gmbh. Plant gene expression, controlled by constitutive plant v-atpase promoters
KR100797667B1 (en) * 1999-10-04 2008-01-23 메디카고 인코포레이티드 Method for regulating transcription of foreign genes
DE60130866T2 (en) * 2000-01-28 2008-07-17 The Governors Of The University Of Alberta, Edmonton ROOT-SPECIFIC EXPRESSION OF TARGET GENES IN PLANTS
RU2333245C2 (en) * 2003-07-17 2008-09-10 Адзиномото Ко., Инк. Methods for cultivating plants with improved growth rate under limited nitrogen level
CN101378651B (en) * 2005-12-23 2012-03-14 阿凯迪亚生物科学公司 Nitrogen-efficient monocot plants
DK1968372T3 (en) * 2005-12-23 2012-05-07 Arcadia Biosciences Inc Promoter sequence obtained from rice as well as methods of use
US8889950B2 (en) 2006-07-28 2014-11-18 The George Washington University Repression of AtGLR3.2 increases plant biomass
CN101573727A (en) * 2006-12-06 2009-11-04 阿凯迪亚生物科学公司 Trading greenhouse gas emission credits determined based on the use of a genetically modified plant
CA2671522A1 (en) * 2006-12-06 2008-06-12 Arcadia Biosciences, Inc. Trading greenhouse gas emission credits determined based on the use of a genetically modified plant
US20100318455A1 (en) * 2006-12-06 2010-12-16 Arcadia Biosciences, Inc. Trading nitrogen credits determined based on the use of a genetically modified plant
WO2010046422A2 (en) 2008-10-22 2010-04-29 Basf Se Use of auxin type herbicides on cultivated plants
WO2010046423A2 (en) 2008-10-22 2010-04-29 Basf Se Use of sulfonylurea herbicides on cultivated plants
WO2011089071A2 (en) 2010-01-22 2011-07-28 Bayer Cropscience Ag Acaricide and/or insecticide active substance combinations
MX2013004878A (en) 2010-11-02 2013-07-02 Bayer Ip Gmbh N-hetarylmethyl pyrazolylcarboxamides.
MX2013005258A (en) 2010-11-15 2013-07-05 Bayer Ip Gmbh N-aryl pyrazole(thio)carboxamides.
EP2658853A1 (en) 2010-12-29 2013-11-06 Bayer Intellectual Property GmbH Fungicide hydroximoyl-tetrazole derivatives
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
JP2015532274A (en) 2012-10-01 2015-11-09 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ Use of N-thio-anthranilamide compounds in cultivated plants
WO2014079820A1 (en) 2012-11-22 2014-05-30 Basf Se Use of anthranilamide compounds for reducing insect-vectored viral infections
EP3028573A1 (en) 2014-12-05 2016-06-08 Basf Se Use of a triazole fungicide on transgenic plants
WO2016091674A1 (en) 2014-12-12 2016-06-16 Basf Se Use of cyclaniliprole on cultivated plants
CA2980505A1 (en) 2015-04-07 2016-10-13 Basf Agrochemical Products B.V. Use of an insecticidal carboxamide compound against pests on cultivated plants
EP3338552A1 (en) 2016-12-21 2018-06-27 Basf Se Use of a tetrazolinone fungicide on transgenic plants

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990013633A1 (en) * 1989-05-03 1990-11-15 The Trustees Of Rockefeller University Plant asparagine synthetase
WO1991004325A1 (en) * 1989-09-15 1991-04-04 The General Hospital Corporation A membrane associated protein from plants which has nitrate reductase activity
WO1993007279A1 (en) * 1991-10-03 1993-04-15 Smart Plants International, Inc. Inducible plant defense gene regulatory regions from potato and rice, uses thereof, and assays
WO1995009911A1 (en) * 1993-10-06 1995-04-13 New York University Transgenic plants that exhibit enhanced nitrogen assimilation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990013633A1 (en) * 1989-05-03 1990-11-15 The Trustees Of Rockefeller University Plant asparagine synthetase
WO1991004325A1 (en) * 1989-09-15 1991-04-04 The General Hospital Corporation A membrane associated protein from plants which has nitrate reductase activity
WO1993007279A1 (en) * 1991-10-03 1993-04-15 Smart Plants International, Inc. Inducible plant defense gene regulatory regions from potato and rice, uses thereof, and assays
WO1995009911A1 (en) * 1993-10-06 1995-04-13 New York University Transgenic plants that exhibit enhanced nitrogen assimilation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Plant Mol. Biol. Vol. 24 1994. Muench D G & Good A G pages 417-427 *
Plant Mol. Biol. Vol. 27 1995. Stroeher V L et.al. pages 541-551 *

Also Published As

Publication number Publication date
WO1997030163A1 (en) 1997-08-21
AU1586897A (en) 1997-09-02
GB9817804D0 (en) 1998-10-14
AU727264B2 (en) 2000-12-07
GB2325232B (en) 2000-11-29

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PE20 Patent expired after termination of 20 years

Expiry date: 20170213