GB2289277A - Taxane derivatives - Google Patents

Taxane derivatives Download PDF

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GB2289277A
GB2289277A GB9509353A GB9509353A GB2289277A GB 2289277 A GB2289277 A GB 2289277A GB 9509353 A GB9509353 A GB 9509353A GB 9509353 A GB9509353 A GB 9509353A GB 2289277 A GB2289277 A GB 2289277A
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taxane derivative
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taxane
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Nicola Mongelli
Maria Menichincheri
Bassini Domenico Fusar
Marina Ciomei
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Pfizer Italia SRL
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Pharmacia SpA
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D305/00Heterocyclic compounds containing four-membered rings having one oxygen atom as the only ring hetero atoms
    • C07D305/14Heterocyclic compounds containing four-membered rings having one oxygen atom as the only ring hetero atoms condensed with carbocyclic rings or ring systems
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/55Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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Description

2289277 UNSATURATED TAXANE COMPOUNDS The present invention is directed to
taxane derivatives endowed with antitumor activity, to a process f or their preparation and to pharmaceutical compositions 5 containing them.
The taxane family of diterpenes includes Paclitaxel (also named taxol in several publications), isolated and characterized from an extract of bark of Taxus brevifolia L., and Cephalomannine (see J. Chem. Soc. Chem. Comm. 102, 1979); other taxane analogues are also known and were prepared by semisynthesis starting from 10-deacetyl baccatin III, extracted from the needles of Taxus baccata L. (see Wani et al., J. Am. Chem. Soc. 93, 2325, 1971; Lovelle et al., Proc.
Am. Assoc. Cancer Res. 31, 417, 1990).
Particularly, taxol is a very potent anticancer drug and is already applied with success to the treatment of platinum-resistant ovarian cancer. Nevertheless there is a continuous need f or more potent compounds having the broadest possible spectrum of activity on different cancer types.
The present invention provides taxane derivatives modified at the 6-7 positions of the taxane skeleton (taxol numbering). More especially, the invention provides 2lepi taxane derivatives of the formula Ia:
OR 2 0 H 0 OH PhCOO R1CONH 0 Ph Ow- 0Ac Ia wherein R, represents OR or R wherein R is Cl-C5 alkyl, C2-C5 alkenyl or C6-Clo aryl and R2 represents H or Ac (CH3C0). The hydroxy group at 21- position is in the fl configuration.
The alkyl and alkenyl groups may be straight chain groups or branched chain groups. A Cl-C5 alkyl group may be methyl, ethyl, n-propyl, isopropyl, n-butyl, tert-butyl or npentyl. A C2-Ci alkenyl group may be ethenyl, propenyl, 1methyl-l-propenyl or butenyl. A C6-Clo aryl group may be phenyl or naphthyl, for example a-naphthyl or 0- naphthyl. Preferably, R, represents phenyl, tertbutoxy (t-ButO), 1methyl- l-propenyl or n-pentyl. A preferred compound of the invention is 7-deoxy- taxol-6-ene.
The present invention also provides a process for the preparation of taxane derivatives of formula la and their epimers at 21 position. In fact structures of formula la can be obtained by an elimination process from a taxane derivative with a suitable leaving group at the 7-position (like triflate, mesylate, etc.) and with a proper protecting group at the 21-position (like the acetyl group).
The compounds of formula I are endowed also with antitumor activity.
11 v Accordingly, the present invention provides a process for preparing a taxane derivative of formula I 0 OR2 0 R NH 0 H H 0 0Ac OH 0COPh wherein R,, R2 are as defined above, the process comprising carrying out an elimination reaction on a protected taxane de-ivative of formula II:
0 OR2 0 R3 R NH 0 R3 1OR 4 0 OAC OH 0COPh II wherein R, and R2 are as defined above, R3 is a leaving group and R4 is a hydroxy protecting group, thereby to form a said taxane derivative of formula I wherein the 21-hydroxy group carries a said hydroxy protecting group R4; and carrying out the following steps in any order:
a) separating the resulting isomers which are in the a and 0 configuration at the 21-position; and b) removing the said hydroxy " protecting group R4.
Particularly, the compounds of formula Ia according to the - 4 present invention are obtained by separating the desired isomer in the fl cofiguration.
The leaving group R3 can therefore be CH3S020-, CF3S020- or another suitable leaving group. R4 may be Ac, -OCOCH2Ph, -OCOCH2CH=CH2, (i-Pr)3Si, t-BuMe2Si-, t-BuPh2Si- or another suitable hydroxy protecting group. The wavy line denotes that the R.3 group linked at the 7-position of the taxane structure may be in the a or 0 configuration.
The elimination reaction is typically achieved by reacting a compound of formula II with a base. The elimination reaction may thus be performed in the presence of a base such as MeSM, MN3, MM, M2C03, AcOM, etc (wherein M represents an alkali metal such as Na or K either in a suitable polar aprotic organic solvent like dimethylformamide, dimethylsulf oxide, CH3CN, etc. or under phase transfer catalysis conditions in the presence of a quaternary ammonium salt (for example n-BU4NHS04) and in an apolar organic solvent (for example toluene, benzene, methylene chloride, chloroform, etc). The reaction temperature may vary from OOC to 1200C, for example from 0 to 300C.
The removal of the hydroxy protecting group R4 can be carried out under standard conditions such as hydrolysis or hydrogenolysis or utilising tetrabutylammonium fluoride for silyl groups. When the protecting group is Ac, it may be removed by treatment with sodium bicarbonate in MeOH: H20 as reaction medium or with diethylamine in methanol. Removal of the hydroxy protecting group R4 yields compounds of f ormula I. The separation of the isomers which are a and fl at the 21-position may be carried out by analogy with known methods. Preferably, the separation is carried out after removal of R4 protecting group. The separation may be carried out by means of liquid chromatography, preferably on silica gel.
Taxane derivatives of formula II wherein R3 is either CH3S620- or CF3S020in the a configuration are novel and form part of the invention.
These taxane derivatives according to the invention can be obtained reacting a compound of formula III:
OR 0 0 2 OH 0 H H 0 0Ac OH 'o R1 NH 0 - 011109.
1 OR 4 ocoph wherein R,, R2 and R4 are as defined, above with a compound of formula IV:
CX3S02-Y iv wherein X is H or F and Y is a leaving group. The leaving group Y may be halogen (e.g. Cl) or -OSO2CX3 or another 25 suitable leaving group.
Compounds of formula II in fact can be generally obtained by reacting compounds of formulae III-having the hydroxy group at 7 position in a or P configuration---and IV in the presence of a base (for example pyridine, dimethylaminopyridine, diisopropylethylamine, etc) in a suitable organic solvent which can be pyridine itself, CH3M, CH2C1,, etc. The reaction temperature may vary from room temperature to 700C. The reaction time may vary from 1 to 12hrs.
Compounds of formula III practically are taxane derivatives with a 21-hydroxy protecting group. Several examples are already known in the literature. For instance the compound of f ormula III where R, is phenyl, R2 and R4 are both Ac and the 7-OH conf iguration is 0 (i. e. 2 1 -acetyltaxol) is described in Bioch. Bioph. Res. Comm. 124, 329 (1984).
The compound of f ormula III where R, is phenyl, R2 is Ac, R4 is -OCOCHPh and the 7-OH configuration is 0 is reported in Tetrah. 49, 2805 (1993) and the same compound where the 7-OH configuration is a is reported in Tetrah. Lett. 34, 6845 (1993).
Analogously the compound of formula III wherein R, is phenyl, R, and R4 are both Ac and the 7-OH configuration is a - (i. e. 2.' -acetyl-7-epitaxol) may be obtained, starting f rom 7-epitaxol already known in the literature (see J. Nat. Prod.
49, 665-9 (1986)). other compounds of formula III are known compounds or may be prepared by known methods f rom known compounds, see for example W09323389-A.
01 BIOLOGICAL ACTIVITY The cytotoxic activity of the compounds has been evaluated on B16-FlO murine melanoma cell line which was responsive to taxol and on UV2237 murine fibrosarcoma cell line which was less responsive to taxol (A). The mode of action of the compound was also tested on the tubulin assembly- disassembly assay in comparison with taxol (B).
(A) In vitro drug sensitivity assay Exponentially growing B16-FlO murine melanoma cells were seeded (2xl 04/M1) in RPMI 1640 medium supplemented with 10% heat- inactivated fetal calf serum and 2 mM glutamine in 24-well plates (Costar). Exponentially growing UV2237 murine f ibrosarcoma cells were seeded (2 X104/M1) in E-MEM medium with non-essential aminoacids and Na-pyruvate supplemented with 1% vitamins, 2mM glutamine and 10% heatinactivated fetal calf serum in 24-well plates (Costar). Scaled concentrations of tested compounds were added immediately after seeding.
The inhibition of cell growth was evaluated by counting cells with a Coulter counter after 24hrs incubation.
For each tested compound concentration triplicate cultures were used. The antiproliferative activity of the tested compounds was calculated from dose-response curves and expressed as IC50 (dose causing 50% inhibition cell growth in treated cultures relative to untreated controls). The results are shown in Table I.
(B) Microtubule assembly and disassembly assay Calf brain tubulin was prepared by two cycles of assembly-disassembly (Shelanski M.L., Gaskin F. and Cantor C.R., Proc. Natl. Acad. Sci. U.S.A. 70, 765-768, 1973) and 5 stored in liquid nitrogen in MAB (0.1 M MES, 2.5 mM EGTA, 0.5 mM MgSO4 0. 1 mM EDTA, 0. 1 mM DTT pH 6. 4). All the experiments were carried out on protein stored for less than 4 weeks. Before each experiment, the tubulin was kept 30 min at 40C. Assembly was monitored by the method of Gaskin et al. (Gaskin F., Cantor C.R. and Shelanski M.L., J. Molec. Biol. 89, 737758, 1974). The cuvette (1 cm path) containing tubulin (lmg/ml) and 1 mM GTP was shifted to 370C and continuous turbidity measurements were made at 340 nm on a Perkin-Elmer 557 double wavelength double beam spectrophotometer equipped with an automatic recorder and a thermostatically regulated sample chamber. After 30 minutes, 4 mM CaC12 was added and depolymerisation was measured for 10 minutes as decreased turbidity. At regular intervals of 15 minutes scaled doses of the tested compounds were added and variations in the turbidity were monitored. Data are expressed as percentage of repolymerisation induced by the tested compounds. The results are shown in Table I.
F7 TABLE 1 (B) Tubulin (A) CYtotoXicitV Assembly dose: I C.50 MM) 0. Sym SyM B16P10 UV2237 compound prepared in 24 66 4 1 5 2 Example 4
Compound prepared in 21 4 100 5 40 6 249 41 Example 5
Paclitaxel 39 2 93 3 36 10 452 78 (reference compound) j The taxane derivatives of formulae Ia and II are thus antitumor agents. A human or animal suffering from a tumor may thus be treated by a method which comprises the administration thereto of an effective amount of a taxane derivative of formula Ia or II according to the invention. The condition of the human or animal may thereby be improved.
Examples of tumors that can be treated are sarcomas, carcinomas, lymphomas, neuroblastomas, melanomas, myelomas, Wilms tumor, leukemias and adenocarcinomas. The taxane derivatives of formulae Ia and II can be used to treat ovarian cancer, for example platinum-resistant ovarian cancer, metastatic breast cancer, non-small cell lung cancer, and head and neck cancer.
The invention also provides a pharmaceutical composition which comprises, as active ingredient, a compound of formula Ia or II according to the invention and a pharmaceutically acceptable carrier or diluent. The composition of the invention is usually prepared following conventional methods and is administered in a pharmaceutically suitable form.
concentration, Administration can be made by any of the accepted ways for administration of antitumor agents such as intravenous, intramuscular or subcutaneous injection or topical application. For systemic injection the active compound may be, e.g. dissolved in a vehicle consisting polyoxyethylated castor oil (Cremaphor EL) 50% and ethanol 50% and then diluted with glucose 5% solution at the desired or other pharmaceutically suitable carriers.
The amount of the active compound administered depends on the treated subject, for example age, weight and sex; and the severity of the affliction. The method of administration depends on the judgement of the prescribing physician. A suitable dosage for an average 70 kg may range from about 0.01g to about 1g per day.
The following Examples illustrate the invention but they are not intended to limit it thereto. 7-Epitaxol was prepared according to a literature method (Tetrah. Lett. 34, 6845, 1993).
ExaLnRle 1 V-Acetyl-7-ePitaxol 539mg (0.631 mmol) of 7-epitaxol in pyridine (4mL) under nitrogen were treated with acetic anhydride (295gL, 3.13 mmol) at 01>C. The reaction mixture was stirred at OOC for 1hr, then poured into ice-water and extracted twice with a- :1 - 11 ethyl acetate. The organic phase was washed once with 1N HCl, once with brine and then dried over sodium sulphate, filtered and evaporated under vacuum, yielding 539mg (95%) of the title compound as a white crystalline solid.
H NMR (CDC13, 400 MHz) 1. 14 (S, 3H, 1.18 '(S, 1.67 1.77 1.90 2.14 2.0 2.54 3.71 3.93 4.39 4.70 4.94 5.56 5.76 5.98 6.22 6.82 6.90 7.3 - CH3-16) 3H, CH3-17) (S, 3H, CH3-19) (s, 1H, OH-1) (d, J= 1. 2 Hz, 3H, CH3- 18) 2.19 (two singlets, 6H, CH3CO-21+ CH3CO-10) 2.4 (m, 4H, CH2-14 + CH2-6) (S, 3H, CH3CO-4) (ddd, J = 11.7 Hz, J = 5.0 Hz, J = 2.0 Hz, 1H, H-7) (d, J = 7.5 Hz, 1H, H-3) (s, 2H, CH,-20) (d, J = 11.7 Hz, 1H, OH-7) (dd, J = 3.5 Hz, J = 9.1 Hz, 1H, H-5) (d, J = 3.2 Hz, 1H, H-2f) (d, J = 7.5 Hz, 1H, H-2) (dd, J = 3.2 Hz, J = 9.4 Hz, 1H, H-3f) (m, 1H, H-13) (s, 1H, H-10) (d, J 9.4 Hz, 1H, NH) 8.2 1SH, 3 Ph) Example 2 2f-Acetvl-7-epi-methanesulphonvltaxol To a solution of 404mg (0.451 m-mol) of 21-acetyl-7-epitaxol in pyridine (6mL) under nitrogen were added dimethylaminopyridine (55mg, 0.45 mmol) and, dropwise at OOC, methanesulphonylchloride (882gL, 11.39 mmol). The reaction mixture was allowed to warm to room temperature and then heated at 500C for 18hrs. The reaction mixture was poured into ice-water, extracted with ethyl acetate, the organic phase washed once with 1N HCl, with brine, dried over sodium sulphate, filtered and evaporated under vacuum. The crude mixture was purified by flash chromatography over silica gel (eluant: n-hexane/ethyl acetate = 1/1) yielding 298mg (68%) of the title compound as a white solid. 42.5mg (11%) of the starting material were recovered.
H NMR (CDC'3, 400 MHz) 1.16 (s, 3H, CH3-16) 1.20 (s, 3H, CH3-17) 1.77 (s, 3H, CH3-19) 2.01 (d, J= 1. 2 Hz, 3H, Cl3-18) 2.15, 2.19 (two singlets, 6H, CH3CO-2 + CH3CO-10) 2.1 - 2.3 (in, 2H, CH-14 + CH-6) 2.49 (s, 3H, CH3CO-4) 2.51 (dd, J = 15.2 Hz, J = 9.4 Hz, 1H, CH-14) 3.08 (ddd, J = 16.4 Hz, J = 9.1 Hz, J = 2.9 Hz, 1H, CH-6) 3.25 (s, 3H, CH3S02-) 1 4.09 (d, J = 7.3 Hz, 1H, H-3) 4.33, 4.54 (two doublets, J = 8.5 Hz, 2H, CH2-20) 4.70 (dd, J = 2.9 Hz, J = 2.9 Hz, 1H, H-7) 5.04 (dd, J = 5.6 Hz, J = 9.1 Hz, 1H, H-5) 5.54 (d, J = 2.9 Hz, 1H, H-2f) 5.76 (d, J = 7.3 Hz, 1H, H-2) 6.01 (dd, J = 2.9 Hz, J = 9.4 Hz. 1H. H-31) 6.25.(m, 1H, H-13) 6.49 (sf 1H, H-10) 6.88 (d, J = 9.4 Hz. 1H, NH) 7.3 - 8.2 (m. 15H, 3 Ph) Example 3
2f-Acetvl-7-deoxy-taxol-6-ene To a solution of 2,1 -acetyl-7-epimethanesulphonyltaxo1 (391mg, 0.127 mmol) under nitrogen in N,N-dimethylformamide (8mL) was added sodium azide (330mg, 5.075 mmol). The reaction mixture was stirred at about 900C for 5hrs, then treated with water and ethyl acetate. The organic phase was washed twice with water, once with brine, dried over sodium sulphate, filtered and evaporated under vacuum. The crude mixture was purified by chromatography over silica gel (eluant:n-hexane/ethyl acetate = 1/1) yielding 128mg (36%) of the title compound.
'H NMR (CDC13, 400 MHz) 1.14 (s, 3H, CH3-16) 1.24 (s, 3H, CHI-17) 1.85 1.87 2.14, 2.1 5 2.44 4.02 4.32, 5.12 5.51 5.86 5.95 6.07 6.23 - 14 (d, J= 1. 2 Hz, 3H, CH3-18) (s, 3H, CH3-19) 2.22 (two singlets, 6H, CH3CO-2f + CH3CO-10) 2. 5 (m, 2H, CH2-14) (s, 3H, CH3CO-4) (d, J = 6.2 Hz. 1H, H-3) 4.44 (two doublets, J = 8.5 Hz. 2H, CH2-20) (d, J = 5.6 Hz, 1H, H-5) (d, J = 3.1 Hz, 1H, H-21) (mf 2Hf H-2 + H-7) (dd, J = 3.1 Hz, J = 9.1 Hz, 1H, H-3f) (dd, J = 5.6 Hz. J = 10.0 Hz, 1H, H-6) (m, 2H, H-13 + H-10) 6.89 (d, J = 9.1 Hz, 1H, NH) 7.3 - 8.2 (m, 15H, 3 Ph) Example 4 7-Deoxy-taxol-6-ene To a suspension of 21-acetyl-7-deoxy-taxol-6-ene (21mg, 0.024 mmol) in MeOH: H,0 = 9: 1 (SmL) was added sodium bicarbonate (Smg, 0. 059 mmol). The reaction mixture was stirred for 3hrs at room temperature and then kept for 24hrs at OOC. The reaction mixture was diluted with water, extracted with ethyl acetate, the organic phase washed with water, brine and dried over sodium sulphate. The crude material was purified by chromatography over silica gel (eluant: n-hexanelethyl acetate = 1/2) yielding 9.5mg (47%) 1 of the title compound.
Rf, 0.29 (n-hexanelethyl acetate 1/1) FAB-MS:m/z 834, [M-H]- H NMR (CDC13, 400) 5 1.15 1.24 1.69 1.87 2.23 2.2 2.39 3.56 4.00 4.33 4.78 5.10 5.80 5.84 5.87 6.06 6.20 7.02 (S, 3H, CH3-16) (S, 3H, CH3-17) (d, J= 1. 5 Hz, 3H, CH3-18) (S, 3H, CH3- 19) (S, 3H, CH3CO-10) 2. 5 (m, 2H, CH,-14) (S, 3H, j = CH3CO-4) (d, 4.4 HZ, 1H, OH-21) (d, J = 6.5 Hz, 1H, H-3) 4.43 (two doublets, J = 8.5 Hz, 2H,CH2-20) (dd, J = 4.4 Hz, J = 2.5 Hz, 1H, H-2f) (d, (dd (d, (d, (dd, (m, (d, 7.-2-8.2 J 5.6 Hz.
J 2.5 Hz, J=6.5 Hz, 1H, J=10.0 HZ, 1H, H-7) J=10.0 Hz, J=5.6 2H, H-10+H-13) J=8.8 HZ, 1H, NH) (m, 15H, 3 Ph) 1H, H-5) J = 8.8 Hz, 1H, H-3t) H-2) Hz, 1H, H-6) Example 5 7-Deoxy-21-epi-taxol-6-ene 21-Acetyl-7-deoxy-taxol-6-ene (139 mg, 0.155 mmole) was dissolved in a mixture of methanol (10 ml) and methylene chloride (1.5 ml). A 1% methanolic solution of diethylamine was added and the reaction mixure stirred at room temperature for 1 hour.
The reaction mixture was poured into water (100 ml) and extracted with methylene chloride (3 x 30 ml).
The joined organic layers were washed with water, concentrated and purified on preparative silica gel TLC, eluating with hexane/ethylacetate 1:1.
7-Deoxy -taxol-6-ene (78 mg, 60% yield) and the more polar 7 deoxy-2f-epi-taxol-6-ene (mg. 21, 15% yield) was obtained.
Rf, 0.18 (n-hexane/ethyl acetate=111) FAB-MS:m/z 834, [M-H] H-NMR (400MHz.CD "1 1.12 1.19 (s, 3H, 17) 1.49 (d, J=1.2 Hz, 3H, 18) 1.79 (s, 1H, OH-1) 1.84 (s, 1H, 19) 2.1-2.3 (m, 2H, M-14) 2.21 (s, 3H, CH3CO-10) 2.42 (3,3H,CH3CO-4) 3.44 (bs, 1H, OH-21) 3.98 (d, J=6.6 Hz, 1H, 3) 4.27, 4.43 (two doublets, J=7.9 Hz, 2H, CH2-20) 4.87 (d, J=3.7, 1H, 2f) 3H, 16) 7 S - 17 5.12 (d, J=5.6 Hz, 1H, 5) 5.75 (dd, J=3.7 Hz, J=8.4 Hz, 1H, 31) 5.81 (d, J=6.6 Hz, 1H, 2) 5.84 (d, J=9.7 Hz, 1H, 7) 6.07 (dd, J=9.7 Hz, J=5.6 Hz, 1H, 6) 6.09 (m, 1H, 13) 6.16 (s, 1H, 10) 7.09'(d, J=8.4 Hz, 1H, NH-41) 7-2-8.2 (M,1SH, 3-0) - 18

Claims (11)

1. A taxane derivative of formula Ia OR 2 0 R1CONH 0 Ph H 0 OH 0Ac OH PhCOO 10wherein R, represents OR or R wherein R is' C,-C5 alkyl, C2-C5 alkenyl or C,-C,,) aryl and R2 represents H or CH3CO.
2.
A compound according to claim 1, wherein R, represents phenyl, tert.butoxy, pentyl.
1-methyl-l-propenyl or n- A compound according to claim 1, which is Vepi-7-deoxy-taxol-6-ene.
4. A process for preparing a taxane derivative of formula I 0 )L, R. NH 0 2-1 OR 2 0 ---2 H 0 H 0Ac OH 0COPh I - 19 the process comprising carrying out an elimination reaction on a protected taxane derivative of formula II wherein R, and R, are as def ined in claim 1, R3 is a leaving group and R4 is a hydroxy protecting group, thereby to form a said taxane derivative of formula I wherein the 21-hydroxy group carries a said hydroxy protecting group R4; and carrying 15out the following steps in any order:
a) separating the resulting isomers which are in the a and P configuration at the 21-position; and b) removing the said hydroxy protecting group R4.
5. A taxane derivative of formula II as defined in claim 4 characterized in that R3 is CH3S020 or CF3S020 in the a configuration.
6. A process for preparing a taxane derivative of the-formula II as defined in claim 5, which process comprises reacting a compound of formula III:
OR 2 0 )L1 R 3 R NH 0 OR H 0 4 Z OH 1 0Ac 0COPh II 0 R,,,-kMH OR? H 0 0 J OR4 H DAC OH 0COPh III wherein R,,, R2 and R, are as def ined in claim 4, with a compound of formula IV:
CX3S02-Y iv wherein X is H or F and Y is a leaving group.
7. A pharmaceutical composition which comprises, as active ingredient, a compound of the formula Ia or II as defined in claim 1 or 5 and a pharmaceutically acceptable diluent or carrier.
8. A compound of formula Ia or II as defined in claim 1 or 5 for use in a method of treatment of the human or animal body by therapy.
9. A compound as claimed in claim 8 for use as an antitumour agent.
10. A process for preparing a taxane derivative of formula I as defined in claim 4, said process being substantially as hereinbefore described in Examples 3 and 4 taken together or Examples 3 and 5 taken together.
11. A process for preparing a taxane derivative of formula II as defined in claim 5, said process being substantialy as hereinbefore described in Example 2.
GB9509353A 1994-05-09 1995-05-09 Taxane compounds and uses thereof Expired - Fee Related GB2289277B (en)

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WO2001007040A1 (en) * 1999-07-28 2001-02-01 Kobe Natural Products & Chemicals Co., Ltd. Multidrug-resistant cancer overcoming agents and process for producing the same
EP1497275A1 (en) * 2002-04-05 2005-01-19 Natural Pharmaceuticals, Inc. Selective acylation of secondary hydroxyl groups

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Publication number Priority date Publication date Assignee Title
WO1998022451A1 (en) * 1996-11-19 1998-05-28 Daiichi Pharmaceutical Co., Ltd. Taxol derivatives

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WO2001007040A1 (en) * 1999-07-28 2001-02-01 Kobe Natural Products & Chemicals Co., Ltd. Multidrug-resistant cancer overcoming agents and process for producing the same
EP1497275A1 (en) * 2002-04-05 2005-01-19 Natural Pharmaceuticals, Inc. Selective acylation of secondary hydroxyl groups
EP1497275A4 (en) * 2002-04-05 2006-04-12 Natural Pharmaceuticals Inc Selective acylation of secondary hydroxyl groups
US7176325B2 (en) 2002-04-05 2007-02-13 Natural Pharmaceuticals, Inc. Selective acylation of secondary hydroxyl groups

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GB2289277B (en) 1998-08-19
ITMI950872A1 (en) 1996-10-28
GB9509353D0 (en) 1995-06-28
GB9409131D0 (en) 1994-06-29
JPH07304761A (en) 1995-11-21
DE19516631A1 (en) 1995-11-16
ITMI950872A0 (en) 1995-04-28

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