GB2284051B - Quantification of immobilised nucleic acid sequences using enzyme-labelled oligonucleotide probes - Google Patents

Quantification of immobilised nucleic acid sequences using enzyme-labelled oligonucleotide probes

Info

Publication number
GB2284051B
GB2284051B GB9422297A GB9422297A GB2284051B GB 2284051 B GB2284051 B GB 2284051B GB 9422297 A GB9422297 A GB 9422297A GB 9422297 A GB9422297 A GB 9422297A GB 2284051 B GB2284051 B GB 2284051B
Authority
GB
United Kingdom
Prior art keywords
quantification
enzyme
nucleic acid
acid sequences
oligonucleotide probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB9422297A
Other versions
GB9422297D0 (en
GB2284051A (en
Inventor
Stephen Minter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tepnel Medical Ltd
Original Assignee
Tepnel Medical Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tepnel Medical Ltd filed Critical Tepnel Medical Ltd
Publication of GB9422297D0 publication Critical patent/GB9422297D0/en
Publication of GB2284051A publication Critical patent/GB2284051A/en
Application granted granted Critical
Publication of GB2284051B publication Critical patent/GB2284051B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB9422297A 1993-11-04 1994-11-04 Quantification of immobilised nucleic acid sequences using enzyme-labelled oligonucleotide probes Expired - Fee Related GB2284051B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB939322721A GB9322721D0 (en) 1993-11-04 1993-11-04 Determining amounts of nucleic acid

Publications (3)

Publication Number Publication Date
GB9422297D0 GB9422297D0 (en) 1994-12-21
GB2284051A GB2284051A (en) 1995-05-24
GB2284051B true GB2284051B (en) 1998-01-07

Family

ID=10744619

Family Applications (2)

Application Number Title Priority Date Filing Date
GB939322721A Pending GB9322721D0 (en) 1993-11-04 1993-11-04 Determining amounts of nucleic acid
GB9422297A Expired - Fee Related GB2284051B (en) 1993-11-04 1994-11-04 Quantification of immobilised nucleic acid sequences using enzyme-labelled oligonucleotide probes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB939322721A Pending GB9322721D0 (en) 1993-11-04 1993-11-04 Determining amounts of nucleic acid

Country Status (1)

Country Link
GB (2) GB9322721D0 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201003594D0 (en) * 2010-03-04 2010-04-21 Eluceda Ltd Method for detecting the presence of specific micro-organisms and device for the same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008808A1 (en) * 1990-11-14 1992-05-29 Siska Diagnostics, Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
WO1993013220A1 (en) * 1991-12-24 1993-07-08 Tepnel Medical Limited Manipulating nucleic acid sequences

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008808A1 (en) * 1990-11-14 1992-05-29 Siska Diagnostics, Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
WO1993013220A1 (en) * 1991-12-24 1993-07-08 Tepnel Medical Limited Manipulating nucleic acid sequences

Also Published As

Publication number Publication date
GB9422297D0 (en) 1994-12-21
GB9322721D0 (en) 1993-12-22
GB2284051A (en) 1995-05-24

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19991104