GB2259709A - Production of chitin/chitosan - Google Patents
Production of chitin/chitosan Download PDFInfo
- Publication number
- GB2259709A GB2259709A GB9218243A GB9218243A GB2259709A GB 2259709 A GB2259709 A GB 2259709A GB 9218243 A GB9218243 A GB 9218243A GB 9218243 A GB9218243 A GB 9218243A GB 2259709 A GB2259709 A GB 2259709A
- Authority
- GB
- United Kingdom
- Prior art keywords
- stipes
- chitin
- chitosan
- production
- harvested
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
A method for the production of chitin/chitosan comprises harvesting stipes from mushroom growing and processing the harvested stipes e.g. with alkali to remove non-chitinous material therefrom.
Description
PRODUCTION OF CHITIN/CHITOSAN
This invention relates to the production of chitin/chitosan.
Chitin and/or its deacetylated derivative chitosan (herein, chitin/chitosan means the one or the other or a mixture) are useful in numerous applications including wound dressing materials (where there is evidence of proactivity tending to accelerated healing), heavy metal recovery and general filtration.
Traditionally, chitin/ckitosan is harvested from crustacean sheils. More recently, microfungal hyphae have been proposed as a source, the microfungi being grown in culture or, as in the case of sporangiophores, on a culture-bearing substrate, like cress.
The present invention provides a novel source.
The invention comprises a method for the production of chitin/chitosan comprising harvesting stipes from mushroom growing and processing the harvested stipes to remove non-chitinous material therefrom.
The harvested stipes may be processed by treatment in alkali to remove proteinaceous material, which may also deacetylate chitin in the stipes to chitosan.
The alkali may be NaOH, and the stipes may be boiled in NaOH solution typically 2 molar boiled for one hour.
The processed stipes may be recovered as filamentous material, which is useful for incorporation into textile or textile-like structures for use in filters, would dressings, etc.
Methods for the production of chitin/chitosan according to the invention will now be described.
The methods comprise harvesting stipes from mushroom growing. By "mushroom in this context will be ordinarily understood the fungi Agaricus bisporus or
A. campestris, which are produced in large quantities.
But also oyster mushrooms and other edible mushrooms will serve as raw material. The point is that at present the stipes are a waste product of commercial mushroom culture and the invention turns this waste product into useful chitin/chitosan.
The stipes are cut off from the harvested mushrooms, or the mushroom caps cut from the stipes which remain in the mushroom growing compost. In any event, the stipes are washed to free them from any adhering compost.
The washed stipes are then processed by treatment in an alkali, typically 2 molar NaOH, and may be boiled in such solution for half an hour. This treatment will partially deacetylate the chitin as well as remove proteinaceous material - which is highly desirable for example in the case of wound dressings where protein impurities could support bacterial infection. The resulting filamentous product may be washed in weak acid, such as acetic.
On the other hand, the chitin may be completely or more completely converted to chitosan by boiling under pressure or autoclaving in 50% NaOH for, say, four hours. While the product of this treatment is still filamentous, acidification with a weak, e.g. acetic, acid will dissolve it.
Chitosan may be precipitated out by adjustment of the pH back to alkaline. The precipitate can be washed with water to yield a substantially pure chitosan powder.
Claims (6)
1. A method for the production of chitin/chitosan comprising harvesting stipes from mushroom growing and processing the harvested stipes to remove non-chitinous material therefrom.
2. A method according to claim 1, in which the harvested stipes are processed by treatment in alkali to remove proteinaceous material.
3. A method according to claim 1 or claim 2, in ic th chitin in the stipes is deacetylated using alkali.
4. A method according to claim 2 or claim 3, in which the alkali is NaOH.
5. A method according to any one of claims 1 to 4, in which the harvested stipes are boiled in NaOH solution.
6. A method according to any one of claims 1 to 5, in which the processed stipes are recovered as filamentous material.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9218243A GB2259709A (en) | 1991-09-19 | 1992-08-27 | Production of chitin/chitosan |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB919120055A GB9120055D0 (en) | 1991-09-19 | 1991-09-19 | Production of chitin/chitosan |
GB9218243A GB2259709A (en) | 1991-09-19 | 1992-08-27 | Production of chitin/chitosan |
Publications (2)
Publication Number | Publication Date |
---|---|
GB9218243D0 GB9218243D0 (en) | 1992-10-14 |
GB2259709A true GB2259709A (en) | 1993-03-24 |
Family
ID=26299564
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB9218243A Withdrawn GB2259709A (en) | 1991-09-19 | 1992-08-27 | Production of chitin/chitosan |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB2259709A (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996025437A1 (en) * | 1995-02-13 | 1996-08-22 | Abion Beteiligungs- Und Verwaltungsgesellschaft Mbh | Process for producing chitosan-glucan complexes, compounds producible therefrom and their use |
EP1550674A1 (en) * | 2002-10-08 | 2005-07-06 | Ricom Corporation | Chitosan-containing polysaccharide, process for producing the same and use thereof |
FR2908771A1 (en) * | 2006-11-20 | 2008-05-23 | Kitozyme Sa | CHITINE-GLUCAN OF FINE EXTRACT FINE GRANULOMETRY |
US8614070B2 (en) | 2008-07-30 | 2013-12-24 | 73100-Setenta E Tres Mil E CEM, LDA | Process for the co-production of chitin, its derivatives and polymers containing glucose, mannose and/or galactose, by the fermentation of the yeast Pichia pastoris |
US9861700B2 (en) | 2012-03-23 | 2018-01-09 | Pharma73, S.A. | Natural biocomposite powder prepared from pichia pastoris biomass, method of preparation and its use as excipient |
CN107802527A (en) * | 2017-12-12 | 2018-03-16 | 中国药科大学 | Muffin prepared using edible mushroom source combine dressing and preparation method thereof |
US11077060B2 (en) | 2014-05-21 | 2021-08-03 | Pharma73, S. A. | Chitin-glucan complex, its preparation, and uses |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB736708A (en) * | 1952-04-22 | 1955-09-14 | American Home Prod | Improvements in or relating to infant foodstuffs |
GB2026516A (en) * | 1978-06-14 | 1980-02-06 | Muzzarelli Riccardo | Chitinglucan complexes |
JPS5626049A (en) * | 1979-08-06 | 1981-03-13 | Mitsubishi Rayon Co | Chitine or chitine derivative nonwoven fabric and method |
JPS5828243A (en) * | 1981-08-07 | 1983-02-19 | Toyo Seikan Kaisha Ltd | Preparation of mushroom extract |
GB2228259A (en) * | 1988-12-19 | 1990-08-22 | Shinetsu Chemical Co | Separating heavy metal ions from aqueous solution |
-
1992
- 1992-08-27 GB GB9218243A patent/GB2259709A/en not_active Withdrawn
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB736708A (en) * | 1952-04-22 | 1955-09-14 | American Home Prod | Improvements in or relating to infant foodstuffs |
GB2026516A (en) * | 1978-06-14 | 1980-02-06 | Muzzarelli Riccardo | Chitinglucan complexes |
JPS5626049A (en) * | 1979-08-06 | 1981-03-13 | Mitsubishi Rayon Co | Chitine or chitine derivative nonwoven fabric and method |
JPS5828243A (en) * | 1981-08-07 | 1983-02-19 | Toyo Seikan Kaisha Ltd | Preparation of mushroom extract |
GB2228259A (en) * | 1988-12-19 | 1990-08-22 | Shinetsu Chemical Co | Separating heavy metal ions from aqueous solution |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996025437A1 (en) * | 1995-02-13 | 1996-08-22 | Abion Beteiligungs- Und Verwaltungsgesellschaft Mbh | Process for producing chitosan-glucan complexes, compounds producible therefrom and their use |
US6333399B1 (en) | 1995-02-13 | 2001-12-25 | Alexander Teslenko | Process for producing chitosan-glucan complexes, compounds producible therefrom and their use |
EP1550674A1 (en) * | 2002-10-08 | 2005-07-06 | Ricom Corporation | Chitosan-containing polysaccharide, process for producing the same and use thereof |
EP1550674A4 (en) * | 2002-10-08 | 2011-06-15 | Ricom Corp | Chitosan-containing polysaccharide, process for producing the same and use thereof |
JP5102435B2 (en) * | 2002-10-08 | 2012-12-19 | 株式会社リコム | Chitosan-containing polysaccharide, its production method and use |
FR2908771A1 (en) * | 2006-11-20 | 2008-05-23 | Kitozyme Sa | CHITINE-GLUCAN OF FINE EXTRACT FINE GRANULOMETRY |
WO2008061999A1 (en) * | 2006-11-20 | 2008-05-29 | Kitozyme Sa | Fine-granulometry fungal extract chitine-glucane |
US20100003292A1 (en) * | 2006-11-20 | 2010-01-07 | Sandrine Gautier | Fine-granulometry fungal extract chitine-glucane |
US8614070B2 (en) | 2008-07-30 | 2013-12-24 | 73100-Setenta E Tres Mil E CEM, LDA | Process for the co-production of chitin, its derivatives and polymers containing glucose, mannose and/or galactose, by the fermentation of the yeast Pichia pastoris |
US9861700B2 (en) | 2012-03-23 | 2018-01-09 | Pharma73, S.A. | Natural biocomposite powder prepared from pichia pastoris biomass, method of preparation and its use as excipient |
US11077060B2 (en) | 2014-05-21 | 2021-08-03 | Pharma73, S. A. | Chitin-glucan complex, its preparation, and uses |
CN107802527A (en) * | 2017-12-12 | 2018-03-16 | 中国药科大学 | Muffin prepared using edible mushroom source combine dressing and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
GB9218243D0 (en) | 1992-10-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |