GB2212153A

GB2212153A - Phenyl hydroxamic acids

Info

Publication number: GB2212153A
Application number: GB8726186A
Authority: GB
Inventors: Ravi Kannadikovilakom Varma; Eric Michael Gordon; Martin Frederick Haslanger
Original assignee: ER Squibb and Sons LLC
Current assignee: ER Squibb and Sons LLC
Priority date: 1987-11-16
Filing date: 1987-11-09
Publication date: 1989-07-19
Anticipated expiration: 2007-11-09
Also published as: DE3738890A1; GB2212153B; FR2623188A1; GB8726186D0; FR2623188B1

Description

1 QA198 PHENYL M ROXAMIC ACIDS 221215-3 The present invention relates to

phenyl hydroxamic acid derivatives which include at least one hetero-containing substituent and more particularly concerns such derivatives which are inhibitors of arachidonic acid 5- lipoxygenase and as such are useful, for example, as antiallergy and antipsoriatic agents.

In accordance with the present invention new phenyl hydroxamic acid derivatives useful as As-lipoxygenase inhibitors are provided. These new compounds have the general formula I 0 OR2 11 1 20 Z-X- (CE2)M-Y- (CI2)n-( C-N-R, or a pharmaceutically acceptable salt thereof, wherein X is NR, oxygen, sulfur, S 11 (0) 9 or a single bond, and Y is NR, oxygen, sulfur, S (h 9 k QA198 -p or a single bond, where R can be hydrogen or lower alkyl, g can be 1 or 2, and with the proviso that at least one of X and Y is other than a single bond; Z is aryl, aralkyl or cycloalkyl; R, is hydrogen, lower alkyl, cycloalkyl, lower alkenyl or aryl; R2 is hydrogen, lower alkyl, aroyl or acyl; m is 0 to 4; and, n is 0 to 4 carbon atoms. Further in accordance With the present invention, a method for using the above compounds is provided.

The hydroxamic acid derivatives of the present invention where R2 is hydrogen may form salts with alkali metals, such as lithium, sodium or potassium. In addition, the compounds of formula I will form weak salts with dicyclohexylamine or other amines as well as with tris(hydroxymethyl)aminomethane, glucamine and other amines as set out in United States patent 4,294,759. The compounds of-the invention wherein X or Y are NR and wherein Z is 2, 3 or 4-pyridyl will form salts with acids, e.g. hydrochloric acid and the like.

The term "lower alkyll' or 11alkyll' as employed herein by itself or as part of another group includes both straight and branched chain radicals of up to 12 carbons, preferably 1 to 8 carbons, such as methyl, ethyl, propyl, isopropyl, butyl, t-butyl, isobutyl, pentyl, hexyl, isohexyl, heptyl, 4,4- dimethylpentyl, octyl, 2,2,4-trimethylpentyl, nonyl, decyl, undecyl, dodecyl, the various branched chain isomers thereof, and the like as well as such groups including a halo-substituent, such as F, Br, Cl or I or CF3, an alkoxy substituent, an aryl substituent, an alkyl-aryl substituent, a haloaryl substituent, a cycloalkyl 1 QA198 substituent, an alkylcycloalkyl substituent, hydroxy, an alkylamino substituent, an alkanoylamino substituent, an arylcarbonylamino substituent, a nitro substituent, a cyano substituent, a thiol substituent or an alkylthio substituent.

The term Ilcycloalkyll' employed herein by itself or as part of another group includes saturated cyclic hydrocarbon groups containing 3 to 12 carbons, preferably 3 to 8 carbons, which include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclodecyl and cyclododecyl, which groups are substituted with the same, or a different cycloalkyl.

is The term Ilaryll' or 11Arll as employed herein by itself or as part of another group refers to monocyclic or bicyclic aromatic groups containing from 6 to 10 carbons in the ring portion, such as phenyl, 2, 3 or 4-pyridyl, naphthyl, substituted phenyl or substituted naphthyl wherein the substitutent on either the phenyl, pyridyl or naphthyl may be 1 or 2 lower alkyl groups, 1 or 2 halogens (Cl, Br or F), 1 or 2 lower alkoxy groups, 1 or 2 hydroxyl groups, 1 or 2 alkylamino groups, 1 or 2 alkanoylamino groups, 1 or 2 arylcarbonylamino groups, 1 or 2 amino groups, 1 or 2 nitro groups, 1 or 2 cyano groups, 1 or 2 thiol groups and/or 1 or 2 alkylthio groups.

The term IlaralkyllI, Ilaryl-alkyll' or Ilaryl-lower alkyll' as used herein refers to lower alkyl groups as discussed above having an aryl substituent, such as benzyl.

The term "lower alkenyll' or "alkenyll' as employed herein by itself or as part of another group includes an unsaturated hydrocarbon group QA198 having from 2 to 8 carbons and a single carbon-carbon double bond, such as ethenyl, 1-propenyl, 2-propenyl, 1-butenyl, 2-butenyl, 3-butenyl and the like. 5 The term llacyll' as used herein by itself or as part of another group refers to an alkyl carbonyl or alkenyl carbonyl group. The term llaroyll' as used herein by itself or as part of another group refers to an aryl carbonyl group.

The term "halogen" or "halo" as used herein refers to chlorine, bromine, fluorine or iodine with chlorine being preferred.

Preferred are those compounds of the invention wherein X is 0, Y is 0 or NH, Z is phenyl, R, is CH3, R2 is H, m = 2, and n = 0.

The various compounds of the invention may be prepared as described below.

To make compounds of formula I wherein X is NR, R is hydrogen, Y is a single bond and Z is phenyl, a carboxylic acid of the formula 11 0 11 HO(CH2) M-- - C- On in an organic solvent, e.g. dichloromethane and methanol, is added to a solution of ethereal diazomethane to afford a compound of the formula 0 11 HO (CH2) M -<M- C - OCH3.

t C.

QA198 A solution of the compound of formula III and p-toluenesulfonyl chloride in pyridine can be reacted at a temperature of within the range of from about OC to about 25C to obtain a compound of the formula IV 0 0 1 - 11 CHS-(M-S -0 - (CH2) m 11 0 Compound IV can be reacted with aniline and anhydrous sodium bicarbonate in the presence of hexamethylphosphoric triamide (HMPA) at a temperature between about 250C and 700C to provide a compound having the formula v 0 H 11 N- (CH2) %_ -.J%-=3.

Compound V can thereafter be reacted with benzyl bromide, and anhydrous sodium bicarbonate in the presence of HMPA to afford a compound having the formula vi 0 11 (:D_ N-(CH2) m -m- k.-VA-LI3 1 CH2 -(Q) A mixture of compound VI with lithium hydroxide in a solution of dioxane and water produces a carboxylic acid of the formula VII 0 H N - CCH2 C-OH 1 H2 -70) QA198 A chlorinating agent, e.g. oxalyl chloride or thionyl chloride, is added to a mixture of compound VII in a solution of a solvent, e.g. benzene, and a catalytic amount of dimethylformamide at a temperature between about OOC and 250C to produce compound VIII having the formula is VIII 0 N - (CH2) % - L 2 A solution of compound VIII in a solvent, e.g. tetrahydrofuran, can be reacted with a compound of the formula IX OR2 1 HCl - HN-Rl in the presence of an organic base, e.g-. triethylamine, to obtain a compound of the formula X t 0 OR2 11 1 (M- N- (CH2) m -2- C- N-Rl 1 C22 -Z) h QA198 A mixture of compound X and 5% palladium on carbon in a solvent, e.g. methanol, can thereafter be hydrogenated at about room temperature and atmospheric pressure to afford the compounds of the invention having the formula XI 0 OR2 11 1 (M-NH-(CH2)m -M- C -N- R, that is, compounds of formula I wherein X is NR, R is hydrogen, Y is a single bond, Z is phenyl and n = 0.

To make compounds of the invention as in formula XI but where the phenyl is substituted with hydroxy, the compound of formula II can be reacted with N-bromosuccinimide in the presence of triphenyl phosphine and an organic solvent, e.g. benzene, at a temperature in the range between OIC and 251C. This produces a compound of the formula XII 0 Br (C112 which when reacted with a chlorinating agent, e.g. oxalyl chloride, in the presence of an organic solvent, such as benzene, preferably in the presence of a small amount of dimethylformamide at a temperature in the range between OOC and 250C affords a compound of the formula XIII 0 Br(CH2) m w- t-l.

A compound of the formula QA198 XIV HCl - NH2- 0 - CH2 -W can be reacted with a compound of the formula xv R,- Br in the presence of dry HMPA and dry NaHCO3 to obtain a compound of the formula xvi R, / N-O-CII2 -m H Compounds XVI and XIII, each in solution in an organic solvent, e.g. tetrahydrofuran, and in the presence of triethylamine can be reacted in a molar ratio of between about 1:1 and 2:1 and at a temperature within the range from about OOC to 25"C to afford a compound of the formula XVII 0 OCH2 Br(CE2) m C-N-Rl.

The compound of formula XVII is thereafter reacted with paminophenol in the presence of HMPA and dry NaHCO3 under nitrogen at a temperature of between 1 1 h 1 -g- QA198 about OOC and 250C to produce a compound of the formula M I 1 0 OCII2 -m 11 1 OH NH- (CB2) m C-N-Rl.

Compound XVIII can then be reacted in an organic solvent, e.g. methanol, in the presence of 5% palladium on carbon under hydrogen atmosphere to yield compounds of the invention having the formula xix is 0 OR2 11 1 HO NH - (CH2). C- N- R1 that is, compound of formula I wherein X is NR, R is hydrogen, Y is a single bond, Z is phenyl substituted with hydroxy and n = 0.

To obtain compounds of the present invention wherein Z is aralkyl and X and Y are oxygen, alpha-bromophenetole can be reacted with p-hydroxybenzoic acid in the presence of a base such as sodium hydride in an organic solvent such as dimethylformamide in a molar ratio from between about 1:1 to about 1:2, and at a temperature within the range from about OOC to 600C to form a mixture of the compound XX 0 Ii U)- O-CH2- CH2-0 -(M- _ - un and the p-hydroxybenzoic acid. This mixture, when reacted in a solvent, e. g. methanol, and chloroform with dia-zomethane in ether yields an ester compound of the formula -10 QA198 0 0- CH2 - CH2 - 0 A solution of compound M in a solvent, e.g. tetrahydrofuran, can thereafter be subjected to an alkali metal hydroxide such as lithium hydroxide to afford a compound of the formula M 1 0 11 U- 0- CH2 - CE2- 0 -U- C - OH.

Compound MI and a chlorinating agent, e.g. oxalyl chloride in a solvent, e.g. benzene, is treated with a catalytic amount of dimethylformamide under nitrogen at a temperature within the range of from about OOC to about 250C. The acid chloride so-formed can then be dissolved in, for instance, tetrahydrofuran and subjected to a solution of methylhydroxylamine hydrochloride and triethylamine to afford a compound having the formula =II 0 OH 11 1 (-0-- 0 -C12 - CH2- 0 -(M- C - N- C113 that is, a compound of formula I wherein X is oxygen, Y is oxygen and Z is aralkyl.

To obtain compounds of the invention wherein X is a single bond, Y is NR, R is hydrogen, n = 0 and Z is phenyl, a benzoate of the formula 1 i xxiv 0 11 NH2 C - OCH3 can be reacted with benzyl bromide in the presence of anhydrous sodium bicarbonate and a solvent, e.g. dry HMPA, under nitrogen at a temperature within the range of from about OOC to 700C to afford a compound of the formula QA198 XXV 0 11 CH2 - NE C - OCH3.

Compound XXV can be reacted with a halogenated phenylalkane of the formula MVI (Q)-(CH2) m- Halogen (e.g. 1-bromo-3-phenylpropane for the case where m is 3) in a molar ratio of from about 1:1 in the presence of a dry organic solvent, e.g. tetrahydrofuran and a base such as sodium hydride or n-butyllithium in hexane. This reaction, which can be carried out at a temperature within the range of from about OOC to about 250C, affords a compound of the formula XWI 1 0 11 (0 -(CH2)M-N -(iD_ C - OCH3 1 z)- CH2 By subjecting compound XXVII to an alkali metal hydroxide, such as lithium hydroxide in the QA198 presence of water and an organic solvent, e.g. dioxane or methanol, a product of the formula xXVI I 1 0 11 C112)m - N c -OR (CH2 is obtained.

The carboxylic acid XXVIII can thereafter be treated with oxalyl chloride in the presence of a catalytic amount of dimethylformamide and a solvent, e.g. benzene, to produce MIX 0 N H - %-L W(CH2)M_ 1 1 (U- CH2 Compound X= can be reacted with the compound of formula IX in the presence of triethylamine, an organic solvent, e.g. tetrahydrofuran and water at a temperature within the range between about OOC and 25'C to afford compound xXX 0 OR2 1 N =- (CH2)M - N CII - N - R, i (: CH2 This compound can be subjected to 5% palladium on carbon in the presence of hydrogen and an organic 1 1 QA198 solvent, such as methanol, to produce the compound of the invention =I 0 OR2 g 1 (U)- (CE2)M-NH -Z)- - N - R, that is, a compound of formula I wherein X is a single bond, Y is NR, R is hydrogen, Z is phenyl and n = 0.

The compounds of the invention are As-lipoxygenase inhibitors and prevent leukotriene C4 formation in macrophages (Samuelsson, B., Science, Vol. 220, p. 568575, 1983). The is administration of compounds of this invention to humans or animals provides a method for treating allergy of a reagin or non-reagin nature. Asthma is preferably treated but any allergy wherein leukotrienes are thought to be involved as pharmacological mediators of anaphylaxis can be treated. For example, the compounds of this invention can be used for treatment of such conditions as allergic rhinitis, food allergy and urticaria as well as asthma and psoriasis.

An effective but essentially non-toxic quantity of the compound is employed in treatment.

The compounds of the invention can be administered orally, parenterally or topically to various mammalian species known to be subject to such maladies, e.g., humans, cattle, horses, cats, dogs, and the like in an effective amount within the dosage range of about 1 to 100 mg/kg, preferably about 1 to 50 mg/kg and especially about 2 to 25 mg/kg on a regimen in single or 2 to 4 divided daily doses.

QA198 The active substance can be utilized in a composition such as tablet, capsule, solution, suspension cream, ointment or lotion containing about 5 to about 5000 mg per unit of dosage of a compound or mixture of compounds of formula I. They may be compounded in conventional matter with a physiologically acceptable vehicle or carrier, excipient, binder, preservative, stabilizer, flavor, etc. as called for by accepted pharmaceutical practice. Also as indicated in the discussion above, certain members additionally serve as intermediates for other members of the group.

The following examples represent preferred embodiments of the present invention.

1 1 QA198 Example 1

N-Hydroxy-N-methyl-4-[3-(phenylamino)- propyllbenzamide A. 4-(3-Hydroxy propyl)benzoic acid 1.7M n-butyllithium in hexane (19.4 ml, 33 mmole) was added dropwise to a chilled (--780C) and stirred solution of diisopropylamine (4.63 ml, 33 mmole) in 20 ml of dry tetrahydrofuran (hereinafter THF). After 20 minutes, a solution of p-toluic acid (2.042 g, 15 mmole) in 20 ml of dry THF was added dropwise. After stirring at -78C for another 1.5 hours, 4 ml of HMPA was added followed immediately by a solution of ethylene oxide (2.99 g, 67.9 mmole) in 10 ml of dry THF. The resulting solution was stirred at -780C for 2 hours, treated with 5% hydrochloric acid and warmed up to room temperature, the THF being removed in vacuo. The aqueous solution was saturated with sodium chloride and extracted three times with ethyl ether. The combined ether extracts were concentrated from 300 to 100 ml and extracted with a 0.5N sodium hydroxide solution. This extract was acidified with 10% hydrochloric acid and extracted three times with ethyl ether. The ether extracts were dried over anhydrous magnesium sulfate and evaporated to a residue. This residue was chromatographed on a silica gel column, eluting successively with dichloromethane-ethyl acetate (9:1 and 1:1), ethyl acetate and dichloromethane-methanol (9:1) to give 750 mg of the title A compound, with consistent spectral data.

QA198 B. 4-(3-Hydroxy propyl)benzoic acid, methyl ester To a solution of title A compound (750 mg, 4.16 mmole) in a mixture of 50 ml of dichloromethane and 10 ml of methanol was added a solution of ethereal diazomethane until a yellow color persisted. After stirring for 30 minutes, the excess diazomethane was destroyed by a few drops of glacial acetic acid. The solvent was evaporated in vacuo to give 800 mg of title B compound, with consistent spectral data. C. 3-[(4-Methoxy carbonyl)phenyll-propanol p-toluene sulfonic acid ester A solution of title B compound (250 mg, 1.29 mmole) and p-toluenesulfonoyl chloride (493 mg, 2.59 mmole) in 7 ml of dry pyridine was stirred at room temperature under nitrogen for 4 hours. The resulting solution was poured into a cold 10% hydrochloric acid solution, saturated with sodium chloride and extracted three times with ethyl ether. The combined ether extracts were dried over anhydrous magnesium sulfate and evaporated to give 386 mg of title C compound, with consistent spectral data.

D. [N-[3(4-Methoxy carbonyl)phenvllpropyll aniline A mixture of title C compound (1.0 g, 2.87 mmole), aniline (267 mg, 2.87 mmole) and anhydrous sodium bicarbonate (360 mg, 4.3 mmole) in 7 ml of dry HMPA was stirred at 85"C under nitrogen for 7 hours. The resulting solution was cooled to room temperature, diluted with 25 ml of water and extracted with ethyl ether. The ether extract was washed several times with water, dried over anhydrous magnesium sulfate and evaporated to QA198 produce an oil. The oil was chromatographed on a silica gel column to give 500 mg of title D compound, with consistent spectral data, as an oil. E. rN-rN-r3-(4-Methoxy carbonyl)phenyllPropyllbenzyll aniline A mixture of title D compound (1.1 g, 4,08 mmole), benzyl bromide (768 mg, 4.49 mmole) and anhydrous sodium bicarbonate (515 mg, 6.13 mmole) in 15 ml of dry HMPA was stirred at 700C under N2 for 3 hours. The resulting solution was cooled to room temperature, poured into 50 ml of cold water and extracted twice with ethyl ether. The combined extracts were washed, dried over magnesium sulfate and evaporated to give an oil which was chromatographed to yield 1.1 g of title E compound, with consistent spectral data, as a solid.

F. [N[N-[3-(4-Carboxy)phenvllpropyllbenzylI aniline A mixture of title E compound (1.1 g, 3.06 mmole) and lithium hydroxide (1.0 g) in a mixture of 20 ml of dioxane and 10 ml of water was refluxed under nitrogen for 2 hours. The resulting solution G.

was cooled to room temperature, adjusted to a pH of 5.5 with 5% hydrochloric acid and most of the dioxane was removed in vacuo. The residual slurry was saturated with sodium chloride and extracted three times with ethyl acetate. The combined extracts were dried over anhydrous magnesium sulfate and evaporated to give 1.05 g of title F compound, with consistent spectral data, as a solid. [N-[N-r3-(4Chlorocarbonyl)phenyllpropyl]_benzyl] aniline To a chilled and stirred solution of title F 35 compound (1.05 g, 3.04 mmole) in a mixture of 25 ml QA198 of dry benzene and 0.3 ml of dry dimethylformamide (hereinafter DMF) at OOC, under nitrogen, was added dropwise oxalyl chloride (1.5 ml, 17.19 mmole). Thereafter, the solution was stirred at room temperature under nitrogen for 1.5 hours. The solvent was evaporated by a stream of nitrogen. The residue was dried in vacuo at room temperature for 1 hour to afford 1.06 g of title G compound, with consistent spectral data.

H. N-Hydroxy-N-methyl-4-[3-(N-benzyl-N-pheny1 amino)-Propyllbenzamide To a stirred solution of N-methylhydroxylamine hydrochloride (600 mg, 7.18 mmole) in a mixture of 15 ml of THF and 5 ml of water was added triethylamine (4.5 ml, 3.23 mmole).

A solution of title F compound (1.06 g, 2.91 mmole) in 20 ml of dry THF was then added dropwise. The resulting solution was stirred for 16 hours, adjusted to a pH of 5.5 (with 5% hydrochloric acid), most of the THF removed in vacuo, saturated with sodium chloride and extracted three times with ethyl ether. The combined extracts were washed with dilute brine, dried and evaporated to give a gum. The gum was chromatographed to give 920 mg of title H compound, with consistent spectral data, as an oil. I. N-Hydroxy-N-methyl-4-[3-(phenylamino)propyllbenzamide A mixture of title H compound (920 mg, 2.46 30 mmole) and 5% palladium on carbon (100 mg) in 75 ml of methanol was hydrogenated at room temperature under atmospheric pressure for 2 hours. The resulting mixture was filtered, washing with methanol. The filtrate was concentrated in vacuo and chromatographed to afford 250 mg of title I QA198 compound, with consistent spectral data, m.p 93-94'C.

QA198 Example 2

N-Hydroxy--4-[3[(4-hydroxyphenyl)aminol- propyll-N-methylbenzamide A. 4-(3-Bromopropyl)-benzoic acid A complex of triphenyl phosphine (3.16 g, 12 mmole) and N- bromosuccinimide (2.14 g, 12 mmole) was prepared by stirring these in 35 ml of benzene in an ice bath for 10 minutes and at room temperature for 1 hour. A solution of 4-(3-hydroxy propyl)-benzoic acid (1.08 g, 6.0 mmole), prepared as described in Example 1, step A, in 15 ml of dry methylene chloride was added to the complex and the stirring was continued for 30 minutes. The so-formed mixture was then concentrated, diluted with 50 ml of ethyl ether and a solution of sodium carbonate (1.27 g, 12 mmole) in 50 ml of water and stirred vigorously. The ethyl ether layer was separated and the aqueous layer was extracted again with ethyl ether. The aqueous layer was acidified (with 10% hydrochloric acid) and extracted twice with ethyl ether. These extracts were combined, washed, dried, evaporated and chromatographed to afford 1.2 g of title A compound, with consistent spectral data, as a colorless solid, m.p.

116-1170C.

B. 4-(3-Bromopropyl)-benzoyl chloride To a cooled and stirred solution of title A compound (500 mg, 2.05 mmole) in 15 ml of dry benzene was added oxalyl chloride (0.6 ml) followed dropwise by a solution of dry DMF (0.2 ml) in dry benzene (2.0 ml). Following a vigorous gas evolution, the mixture was stirred at room temperature for 1 hour, evaporated and dried to afford 570 mg of title B compound, with consistent spectral data, as a gummy solid.

C. O-Benzyl-N-methyl hydroxylamine QA198 To a stirred solution of 0-benzylhydroxyl amine hydrochloride (1.59 g, 10 mmole) in 15 ml of dry HMPA containing dry sodium bicarbonate (3.36 g, 40 mmole) was added methyl iodide (1.5 g, 11 mmole). After 5 hours the resulting mixture was diluted with 30 ml of water and 20 ml of brine and extracted three times with ethyl ether. The extracts were combined, washed, dried and evaporated to produce the crude product as an oil.

The oil was chromatographed to afford 800 mg of title C compound, with consistent spectral data.

D. N-Benzyloxy-N-methyl-4-(3-bromopropyl)- benzamide A solution of title C compound (548 mg, 4.0 mmole) in dry THF (10 ml) containing triethylamine (1.1 ml, 8.0 mmole) was cooled and stirred in an ice bath. A solution of title C compound (570 mg, -2.05 mmole, crude) in dry THF (10 ml) was added.

A deep purple color developed. After 1 hour, the mixture was diluted with 10% hydrochloric acid (25 ml) and brine (75 ml and extracted three times with ethyl ether. The extracts were combined, washed with brine, dried and evaporated to afford a dark pink colored oil. The oil was chromatographed on a column of silica gel to give 690 mg of title D compound, with consistent spectral data, as a light-purple-colored oil.

E. N-Benzyloxy-4-[3-[(4-hydroxyphenyl)aminol- propyll-N-methyl benzamide A stirred solution of title D compound (690 mg, 1.97 mmole) in dry HMPA (8.0 ml) containing a suspension of dry NaHCO3 (504 mg, 6.0 mmole) was mixed with p-aminophenol (654 mg, 6.0 mmole) and heated under an atmosphere of nitrogen in a bath at QA198 750 for 1.0 hour. The mixture was then cooled to room temperature, diluted with water (50 ml) and extracted twice with ethyl ether. The extracts were combined, washed with water, dried and evaporated to afford the crude product as an oil. The oil was chromatographed on a column of silica gel to give 670 mg of title E compound, with a consistent spectral data, a slightly colored thick oil.

F. N-Hydroxy-4-[3-[(4-hydroxyphenyl)amino]propyll-N-methylbenzamide A solution of title E compound (630 mg, 1.67 mmole) in methanol (30 ml) containing 5% palladium on carbon (50 mg) was stirred under an atmosphere of hydrogen for 1 hour. It was then filtered through a bed of celite, washing with small amounts of methanol. The filtrate and the washings were combined and evaporated to afford a thick oil. The oil was crystallized from ethyl acetate:hexane (7:3) followed by drying o afford 320 mg of title F compound, with consistent spectral data, as a brownish-gray solid, m.p. 152-153'C.

4 Z QA198 Example 3

N-Hydroxy-N-methyl-4-(2-phenoxyethoxy)-.

benzamide A. Mixture of p-hydroxybenzoic acid and 4-(2-Phenoxy ethoxy)benzoic acid A mixture of 50% NaR/paraffin (960 mg, 90 mmole), dry DMF (35 ml) and p-hydroxybenzoic acid (1.2 g, 10 mmole) was heated in a bath at 1200 for minutes resulting in a thick white solid. After dilution with more DMF (20 ml), alpha-bromophenetole (2.01 g, 20 mmole) was added and the heating continued for another 18 hours. Water (5.0 ml) and solid sodium hydroxide (500 mg) were added and the mixture was heated again for 15 minutes. Most of the DMF was then removed by distillation in vacup. The residue was diluted with water (150 ml) and extracted twice with ether. The extracts were discarded. The aqueous layer was acidified with concentrated hydrochloric acid and extracted three times with ethyl acetate. The extracts were combined, washed with brine,dried and evaporated to afford 1.4 g of a mixture of p-hydroxybenzoic acid and 4-(2-phenoxy ethoxy)benzoic acid, with consistent spectral data, (1:3) as a solid.

B. 4-(2-Phenoxy ethoxy)benzoic acid, methyl ester The mixture from step A (1.4 g) was dissolved in a mixture of methanol (10 ml) and chloroform (40 ml) and a slight excess of a solution of diazomethane in ether was added resulting in a very fast reaction. The solution was then evaporated to dryness. The residue was dissolved in ether (100 ml) and stirred vigorously with 1N sodium hydroxide 35 (50 ml) for 1 hour. The ether layer was separated, QA198 washed once with water (10 ml), dried and evaporated to afford 1.0 g of title B compound, with consistent spectral data, as a solid, m.p. 92-930C.

c. 4-(2-Phenoxy ethoxy)benzoic acid A solution of title B compound (1.0 g, 3.98 mmole) in THF (25 ml) containing 1N lithium hydroxide (15 ml) was refluxed under stirring in an atmosphere of nitrogen for 24 hours. The mixture was then concentrated in vacuo, diluted with water (100 ml) and acidified with concentrated hydrochloric acid. The so-treated material was isolated by filtration, washed with water, dried and evaporated to afford 900 mg of title C compound, with consistent spectral data, as a solid, m.p. 198-1990C. D. N-Hydroxy-N-methyl-4-(2-phenoxyethoxy)-. benzamide A solution of title C compound (300 mg, 1.16 20 mmole) and oxalyl chloride (1.5 ml, 16.9 mmole) in dry benzene (7.5 ml) was cooled down to 00, treated with dry DMF and stirred at 00 for 30 minutes under nitrogen and at room temperature for one hour. The excess oxalyl chloride and solvent were removed and the residual solid dried in vacuo for one hour.

This acid chloride was dissolved in dry THF (2.1 ml) and added dropwise with stirring into a cold solution of 98% methylhydroxylamine hydrochloride (204.3 mg, 2.40 mmole) and triethylamine (0.6 ml, 4.88 mmole) in THF (4.5 ml) and water (4.5 ml).

The mixture was stirred at 00 for 30 minutes and at room temperature for 5 hours, diluted with water (15 ml) and extracted twice with dichloromethane - (80 ml). The combined organic extracts were washed with 1N hydrochloric acid (15 ml), 5% sodium QA198 bicarbonate (8 ml) and brine (12 ml), dried, filtered and evaporated to dryness giving 700 mg of the title compound as a solid, with consistent spectral data, m.p. 134-1360.

QA198 Example 4

N-Hydroxy-N-methyl-4-[(3-phenylpropyl)aminoI benzamide A. N-H4-Methoxy carbonY1)phenyl benzylamine A mixture of methyl-4-aminobenzoate (4.535 g, mmole), benzyl bromide (5.134 g, 30 mmole) and anhydrous sodium bicarbonate (3.78 g, 45 mmole) in ml of dry HMPA as stirred at 750 under nitrogen for 6 hours. The resulting reaction mixture was poured into 200 ml of cold water and extracted twice with ethyl ether. The combined ether extracts were washed several times with water, dried over anhydrous magnesium sulfate and evaporated in vacuo to give a solid. This was chromatographed on a silica gel column to give 6.2 g of title A compound, with consistent spectral data, as a solid. B. N- [(4-Methoxy carbonyl)phenyll-N[(3-phenyl)propyll-benzylamine 20 A stirred solution of dry diisopropylamine (1.0 ml, 7.14 mmole) in dry THF (40 ml) was cooled to -780 under nitrogen and 1.65M n-butyllithium in hexane (4. 33 ml, 7.14 mmole) was added dropwise. After 20 minutes a solution of title A compound25 (1.206 g, 5 mmole) in 20 ml of dry THF was added dropwise. The mixture was stirred at -780 for 30 minutes and then gradually warmed up to 00. 1-Bromo-3-phenylpropane (2 ml) was then added immediately, followed by dry HMPA (2 ml). The mixture was then warmed up to room temperature and stirred under nitrogen for 16 hours. Water was added and the THF was substantially removed in vacup. The residual slurry was extracted twice with ethyl ether. The combined ether extracts were washed several times with water, dried over QA198 anhydrous magnesium sulfate and evaporated in vacuo to give a gum. This was chromatographed on silica gel to give 1.03 g of title B compound, with consistent spectral data.

C. N-[(4-Carboxy)phenyl]-N-[(3-jRhenyl)propyllbenzylamine A mixture of title B compound (500 mg, 1.39 mmole) and lithium hydroxide (440 mg, 18.3 mmole) in a mixture of water (5 ml) and dioxane (20 ml) was refluxed under nitrogen for two hours. The resulting reaction mixture was cooled to room temperature, adjusted to pH = 5.0 with 5% hydrochloric acid, saturated with sodium chloride and extracted twice with ethyl ether. The combined ether extracts were dried over anhydrous magnesium sulfate and evaporated in vacuo ' to give 450 mg of title C compound, with consistent spectral data. D. N-[(4-Chlorocarbonyl)phenyl]-N-[(3-phenyl)- propyll-benzylamine To a chilled and stirred solution of title C compound (200 mg, 0.578 mmole) in a mixture of DMF (2 drops) and benzene (3.5 ml) at 00 was added dropwise oxalyl chloride (0.3 ml, 3.44 mmole). After the addition was complete, the solution was gradually warmed to room temperature and stirred under nitrogen for 1 hour. The solvent was evaporated. The residue was dried in vacuo at room temperature for 1 hour to give 205 mg of title D compound, with consistent spectral data.

E. N-Hydroxy-N-methyl-4-[[(3-phe4yl)propylN-benzyll-aminol-benzamide To a solution of N-methyl hydroxylamine hydrochloride (115 mg, 1.37 mmole) and triethylamine (0.85 ml, 6.10 mmole) in a mixture of THF (3 ml) and water (1 ml) was added dropwise a QA198 solution of title E compound (205 mg, 0.56 mmole) in 3 ml of dry.THF. The solution was stirred at room temperature under nitrogen for a few minutes, acidified with 5% hydrochloric acid to pH = 5.5, saturated with sodium chloride and extracted three times with ethyl ether. The combined ether extracts were dried over anhydrous magnesium sulfate and evaporated in vacuo to give a gum. This was chromatographed on a silica gel column to give 185 mg of title E compound, with consistent spectral data. F. NHydroxy-N-methyl-4-[(3-phenylpropyl)amino]-benzamide A mixture of title E compound (95 mg, 0.254 mmole) and 5% palladium on carbon (30 mg) in 10 ml of methanol was hydrogenated at room temperature under one atmospheric pressure for 1.5 hours. The resulting mixture was filtered through a bed of celite and washed with methanol. The filtrate and washing were combined and concentrated in vacuo to give 70 mg of NHydroxy-N-methyl-4-((3-phenylpropyl)amino]-benzamide, with consistent spectral data, m.p. 107-1080.

Z QA198 Examples 5 to 20 The following additional compounds within the scope of the present invention may be prepared by employing the teachings as outlined above and in the working examples. 0 OR2 11 1 C- N- R, Z - X (CH2)m- Y - (CK2)n W W N K) [-A F-A ul 0 (n 0 Ex. No. X y z R 1 R 2 m n -CII 3 - cl - -S- 11 11 --- 0 0 oli-(0 -C-1.1 fV3 2 0 1 W 0 1 )o f 8 CD- 3 3 OD k - 1, [.A bi en C) Ul FK. Db. X y z R 1 R 2 m n -NH- D- cl! 2- CH -- CH 2 -CH 3 3 0 CH2T3 11 2 cil 3 1 - c liso - 2 -N- -CH2C113 6 12 - cii 3 Co- 2 1 1 W 1 o 1 ko 00 W 0 h) (n M 0 F.A tn 1-A 0 Ul E>c. No. X y z R 1 R 2 m n 13 -MIl- -71H- ii -TI CY A 23 14 -0i 2 3 1 3 C1127 H 1 2 16 NH - 2 2 W OD 17 0 -- (W -C:D -CH 3 21 1 W K) F-A Fi 0 (P 0 (n C) (n EX. NO. X y z R 1 R') M n 18 -ill- -111FI- 1 D- -C] -W-2- G!= CH 2 2 1 19 --- -1li- H 2 2 (W -(0 W, (A3 1 -NH_ -- -ai 3 0 2 4 XD F-, W OD

Claims

CLAIMS k

A compound of the formula Z - X - (CH2)M - Y - (CH2).

wherein X is NR, oxygen, sulfur, S 11 (0)gO or a single bond, Y is NR, oxygen, sulfur, S 1 (61)g, QA198 0 OR2 11 1 C-N- RI or a single bond, where R can be hydrogen or lower alkyl, g can be 1 or 2 and with the proviso that at least one of X and Y is other than a single bond; Z is aryl, aralkyl or cycloalkyl; R, is hydrogen, lower alkyl, cycloalkyl, 25 lower alkenyl or aryl; R2 is hydrogen, lower alkyl, aroyl or acyl; m. is an integer from 0 to 4; n is an integer from 0 to 4; or such a compound in pharmaceutically acceptable salt form.
2. A compound of claim 1 wherein X is NR where R is hydrogen, Y is a single bond and Z is phenyl.
3. A compound of claim 1 wherein X is NR where R is hydrogen, Y is a single bond and Z is phenyl substituted with hydroxy.

Z.

1 QA198
4. A compound of claim 1 wherein X and Y are both oxygen and.Z is phenylalkyl.
5. A compound of claim 1 wherein X is a single bond, Y is NR where R is hydrogen and Z is phenyl.

is
6. A compound of claim 1 having the name N-hydroxy-N-methyl-4-[3(phenylamino)propyl]benzamide-
7. A compound of claim 1 having the name N-hydroxy-4-[3-[(4- hydroxyphenyl)amino]propyl]-N- methylbenzamide.
8. A compound of claim 1 having the name N-hydroxy-N-methyl-4-(2phenoxyethoxy)-benzamide.
9. A compound of claim 1 having the name N-hydroxy-N-methyl-4-[(3phenylpropyl)amino]-benzamide.
10. A composition for inhibiting allergic conditions in a mammalian species comprising an effective amount of a compound as defined in claim 1, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier therefor.
11. A method of inhibiting A5-lipoxygenase which comprises administering to the circulatory system of a mammalian host an effective amount of a compound as defined in claim 1 or a pharmaceutically acceptable salt thereof.
12. The method of claim 11 wherein said compound is administered in an amount within the range of from about 1 to about 100 mg/kg.
13. A method for treating asthma in a mammalian species in need of such treatment, which comprises administering to a mammalian host an effective amount of a compound as defined in claim 1 or a pharmaceutically acceptable salt thereof.

QA198
14. A method for treating psoriasis in humans in need of such treatment which comprises administering an effective amount of the compound as defined in claim 1 or a pharmaceutically acceptable salt thereof orally, parenterally or topically.

r Published 1989 atThe Patent Office. State House, 65171 High Holborr.. London WC1R 4TR Further copies maybe obtained from The Patent Office. Sales Brancb. St Mary Cray. Orpington. Ken, BR-5 3RD. Printed by Multiplex techniques ltd, St Mary Cray. Kent. Con. 1/87