GB2187181A - Removal of ethylene glycol from sewage - Google Patents

Removal of ethylene glycol from sewage Download PDF

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Publication number
GB2187181A
GB2187181A GB08700233A GB8700233A GB2187181A GB 2187181 A GB2187181 A GB 2187181A GB 08700233 A GB08700233 A GB 08700233A GB 8700233 A GB8700233 A GB 8700233A GB 2187181 A GB2187181 A GB 2187181A
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GB
United Kingdom
Prior art keywords
ethylene glycol
sewage
arthrobacter
microorganism
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB08700233A
Other versions
GB2187181B (en
GB8700233D0 (en
Inventor
Mark Benyaminovich Tsinberg
Marina Nafkhatovna Nenasheva
Petr Iliich Gvozdyak
Konstantin Ivanovich Lysikov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Volgo Ural Ni I Pi Dobyche I P
Original Assignee
Volgo Ural Ni I Pi Dobyche I P
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SU864006500A external-priority patent/SU1336454A1/en
Priority claimed from SU864005310A external-priority patent/SU1430366A1/en
Application filed by Volgo Ural Ni I Pi Dobyche I P filed Critical Volgo Ural Ni I Pi Dobyche I P
Publication of GB8700233D0 publication Critical patent/GB8700233D0/en
Publication of GB2187181A publication Critical patent/GB2187181A/en
Application granted granted Critical
Publication of GB2187181B publication Critical patent/GB2187181B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1205—Particular type of activated sludge processes
    • C02F3/1231—Treatments of toxic sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Health & Medical Sciences (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Removal Of Specific Substances (AREA)

Abstract

The invention relates to biological purification of sewage containing high concentrations of ethylene glycol. The method comprises treating the sewage containing ethylene glycol and microelements with the sources of phosphorus, nitrogen and a microorganism of genus Arthrobacter. Then said microorganism is cultivated at 20-35 DEG C until the sewage is purified from ethylene glycol. Said method permits biological purification of the sewage containing over 2000 mg/dm<3> of ethylene glycol to a purification rating of 99%. Diethanolamine is used as a cooxidant at concentrations of over 4000 mg/dm<3>. Arthrobacter simplex 667, arthrobacter variablis 667, arthrobacter species 126 and arthrobacter species 127 are given as the strains capable of being used. Microelements are examplified by K<+>, Mg<2+>, Zn<2+>, Fe<2+>, Mn<2+>, Ca<2+>NO2<-> and SO4<2->.

Description

SPECIFICATION Purification of sewage The invention relates to biological purification of sewage from high concentrations of ethylene glycol.
The main object of the present invention is to provide a method for biological purification of sewage with the aid of microorganisms capable of degrading high concentrations of ethylene glycol (over 2000 mg/dm3) The present invention consists in providing a method for biological purification of sewage from ethylene glycol in which, according to the hereinproposed invention, the sewage containing ethylene glycol and micro-elements is inoculated with the sources of phosphorus, nitrogen and microorganism, genus Arthrobacter, then said microorganism is cultivated at 20-350C until said sewage is purified from ethylene glycol.
Owing to the hereinproposed invention it became possible to carry out biological purification of sewage containing over 2000 mg/dm3 ethylene glycol, attaining 99% purification, rating.
It is practicable, according to the present invention, that the microorganism, genus Arthrobacter, should be constituted by strains Arthrobacter simplex 667, Arthrobacter variabilis 667 deposited with the Central Museum of Industrial Microorganisms of the All-Union Research Institute of Genetics and Selection of Industrial Microorganisms, or strains of Arthrobacter species 126, Arthrobacter species 127 deposited with the Museum of Microorganisms-Destructors of the Institute of Colloidal Chemistry and Chemistry of Water named afterA.V. Dumansky, of the Academy of Sciences of the Ukrainian SSR. Arthrobacter series 126 was deposited with the said museum on 13th March 1984 and has the accession number 126.
For purification of sewage containing ethylene glycol in concentrations exceeding 4000 mg/dm3 it is practicable, according to the present invention, to introduce into sewage a source of nitrogen and co-oxidant of ethylene glycol in the form of diethanolamine at a ratio of 1:4-5 to ethylene glycol and to introduce addition- ally a microorganism, genus Pseudomonas.
For purification of sewage containing both ethylene glycol and diethanolamine, it is practicable, according to the present invention, to maintain a ratio of 1 :4-5 of diethanolamine to ethylene glycol in the course of sewage purification.
Further objects and advantages of the present invention will become apparent from the following detailed description of the hereinproposed method for biological purification of sewage from ethylene glycol, and examples of its embodiment.
The present invention presupposes the utilization of new bacteria, genus Arthrobacter capable of degrading intensively the ethylene glycol present in high concentrations (2000 mg/dm3 and over) to its residual content in sewage of 70-100 mg/dm3.
Bacteria of genus Arthmbacter are moving rod bacteria (cocci on third day) and have a positive Gram's staining. They ferment lactase, xylose, fructose, form hydrogen sulphide and ammonia, and grow well on 2% solutions of sodium chloride and 7% solutions of glycerine.
The purification effect in the sewage with high concentrations (2000 mg/dm3) of ethylene glycol is achieved only by the use of the following strains of Arthrobacter genus: Arthrobacter simplex 667, Arthrobactervariabilis 667, deposited with the Central Museum of Industrial Microorganisms of the All-Union Research Institute of Genetics and Selection of Industrial Microorganisms, or strains Arthrobacter species 126,Arthrobac- ter species 127, deposited with the Museum of Microorganisms, Destructors of the Institute of Colloidal Chemistry and Chemistry of Water name afterA.V. Dumansky of the Academy of Sciences ofthe Ukrainian SSR.
The strains of Arthrobacter species 126 and Arthrobacter species 127 have been selected from activated sludhe in runniny water. Growing in a liquid medium with carbons both strains assimilate saccharose,fruc tose, arabinose, insuI#r, glycerin, inositol, sorbitol, mannitol, glucose, dulcitol, raffinose, lactose, ramnose.
These strains did nor grow on xylose and maltose. Growing, they form a film on the surface of liquid media.
The strain Arthrobacter species 126 consumes galactose but slightly.
The strain Arthrobacter species 127 does not assimilate galactose.
The strain Arthrobycter species 126 is u reasepositive.
The strain Arthrobacter species 127 is ureasenegative.
Growing on plain agar both cultures are cream-coloured.
On synthetic media the strain Arthrobacter species 126 is cream-coloured and the strain Arthrobacter species 127 is of an ambercolour.
During the growth of these bacteria the morphology of cells changes, being modified into coccal, rodlike and V-shaped forms.
The claimed method for biological purification of sewage consists in introducing into ethylene-glycoibearing sewage such substances as microelements (K+, Mg2+, Zn2+, Fe2+, Mn2+, Ca2+, NO2-, So42-), sources of phosphorus, e.g. disubstituted sodium phosphate or calcium phosphate, and nitrogen sources, e.g. ammonium nitrate, ensuring the ratio of carbon: nitrogen: phosphorus of 100:5:1, respectively, required for vital activity of the microorganisms.
Then one of the above-cited strains is introduced into sewage, at a density of inoculating material equal to at least 108 cells/ml.
The above-stated microorganism is cultivated at 20-35"C within 24-48,h, approximately, until the sewage is purified of ethylene glycol.
When handling sewage laden with more than 4000 mg/dm3 of ethylene glycol, introduction of microorganisms is preceded by adding an oxidant in the form of diethanolamine in the quantity ensuring the 1 :4-5 ratio of diethanolamine to glycol. Diethanolamine serves also as a source of nitrogen.
However, in this case the sewage being purified should be additionally inoculated with microorganisms, genus Pseudomonas, in the quantity ensuring a 1:5 ratio of Arthrobacter microorganisms to Pseudomonas microorganisms.
The microorganism, genus Pseudomonas, may be used of any species capable of degrading diethanolamine, concentration over 1000 mg/dm3 in the presence of ethylene glycol, concentration above 2000 mg/dm3.
At the gas-processing plantthenaturalgasispurifiedfromsulphurouscomponentsfhydrogensulphide, mercaptans)with an aqueous solution of diethanolamine, concentration 25-30%. The saturated solution of diethanolamine is subjected to thermal desorption for removing sulphurous compounds, then is purified on active carbons in special adsorbers and returned to the production cycle. The sewage formed during recovery contains up to 10000 mg/dm3 of diethanolamine.
This sewage has also to be purified.
The claimed invention permits purifiying the sewage containing ethylene glycol jointly with diethanolamine in which case diethanolamine functioning as a cooxidantwith relation to ethylene glycol, and as a source of nitrogen, is biodegraded itself. However, forthis purpose as we have established the ratio of diethanolam ineto ethylene glycol should be maintained as 1 :4-5.
Example 1 Biological purification ofthe sewage ofthe following composition, mg/dm3: Ethylene glycol - 3300 K+ - 700 Mg2+ - 240 Zn2+ - 2.1 Fe2t - 16 Mn2+ - 2.1 Ca2+ - 8.8 NH4 - 4.3 Pro43~ - 0.3 NO2- - 0.06 5042 - 80.0 pHofsewageisB - 8.5 chemical oxygen demand (mg/dm3) - 5100.
The sewage is supplemented additionally with ammonium nitrogen (150 mg/dm3 NH4NO3) and phosph- orus (350 mg/dm3 Na2HPO4) thus ensuring the ratio of carbon: nitrogen: :phosphorus of 100:5:1 required for vital activity ofthe utilized microorganisms.
The microorganism Arthrobactersimples 667 is introduced into sewage and the latter is biodegraded in a 83dim3 bioreaction vessel by cultivating the microorganisms continuously in the course of 10 days under aerobic conditions. The initial density of inoculum is 30 g/dm3, dilution rate,0.1 h#1, temperature, 250C, air consumption rate 1 dm/h which provides for at least 5 - 7 mg/dm3 content of water-dissolved oxygen.
To determine the curve of growth, the microorganisms, genus Arthrobacter, are inoculated at a density of 1 08 cells/cm3 into 100 ml of culture medium prepared on ethylene-glycol-bearing sewage and grown in Erlen Meyerflasks in the course of 24 h under periodical conditions. The figures of chemical oxygen demand are found by the known methods of process control of sewage purification works, the amount of ethylene glycol is determined by photometric and gaschromatographic methods while the degree of purification ofsewage from ethylene glycol is calculated by the residual concentration of ethylene glycol in per cent of its initial quantity. The parameters of sewage purification are summarized in the Table thatfollows.
Example2 Sewage is purified from ethylene glycol under the same conditions as those laid down in Example 1, but the microorganisms belong to the strain Arthrobactervariabilis 667.
The parameters of sewage purification are laid down in the Table below.
Example3 Sewage is purified from ethylene glycol similarly to Example 1 with the following points of difference.
Initial concentration of of ethylene glycol is 4600 mg/dm3; the sewage waters are additionally mixed with diethanolamine concentration 1150 mg/dm3, in the capacity of a cooxidant of ethylene glycol and of a sole source of nitrogen, the microorganisms being the strain of Arthrobacter species 126 and bacteria of genus Pseudomonas.
The parameters of sewage purification appear in the Table below.
Example4 Sewage is purified from ethylene glycol similarly to Example 3 but the cooxidant of ethylene glycol and the sole source of nitrogen is constituted by diethanolamine contained in sewage in a concentration of 920 mg/dm3 while microorganisms, genus Arthrobacter, are constituted by the strain of Arthrobacter species 127.
The parameters of sewage purification are presented in the Table below.
Table Ethylene Quality of sewage hefore puri- Example glycol fication (mg/dm3) No. to diethan olamine Concentra- Chemical Concent ratio tion of oxygen ration of ethylene demand diethan glycol olamine 1 2 3 4 5 1 - 3300 5100 2 - 3300 5100 3 4:1 4600 8300 1150 4 5:1 4600 8200 920 Example Quality of sewage after Degree ofpuriflca- No. purification mgidm3 tion, %, from Concentra- Concent- Chemi- ethylene dietha tion of ration cal glycol nola ethylene ofdi- oxy- mine glycol ethan- gen ola- demand mine 1 6 7 8 9 10 1 270 - 405 92.9 2 250 - 395 93.4 3 100 15 150 97.8 98.9 4 100 10 150 97.8 99.1 The present invention permits biological purification of sewage containing over 2000 mg/dm3 of ethylene glycol with a purification rating of 99%.

Claims (5)

1. A method of biological purification of sewage from ethylene glycol, consisting in that the sewage containing ethylene glycol and microelements is treated with the sources of phosphorus, nitrogen and a microorganism, genus Arthrobacterthen said microorganism is cultivated at 20-35"C until said sewage is purified from ethylene glycol.
2. A method as claimed in Claim 1,wherein the microorganism of genus Arthrobacter is constituted by the strains Arthrobacter simplex 667, Arthrobacter variabilis 667, deposited with the Central Museum of Industrial Microorganisms of the All-Union Research Institute of Genetics and Selection of Industrial Mic roorganisms or strains Arthrobacter species 126, Arthrobacter species 127, deposited with the Museum of Microorganisms-Destructors of the Institute of Colloidal Chemistry and Chemistry of Water named afterA.V.
Dumansky of the Academy of Sciences of the Ukrainian SSR.
3. A method as claimed in any one of Claims 1,2, wherein the sewage is inoculated with a source of nitrogen and cooxidantof ethylene glycol in the form of diethanolamine at a ratio of 1 :4.5to ethylene glycol and is additionally mixed with microorganism of genus Pseudomonas.
4. A method as claimed in Claim 3, wherein in the sewage containing additionally diethanolamine the ratio of diethanolamineto ethylene glycol is maintained during purification at1 :4.5.
5. A method for biological purification of sewage from ethylene glycol as claimed in any one ofthe preceding Claims realized substantially as set forth in the Specification and Examples.
GB8700233A 1986-01-09 1987-01-07 Purification of sewage Expired - Fee Related GB2187181B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SU864006500A SU1336454A1 (en) 1986-01-09 1986-01-09 Method of biological purification of waste water from ethylene glycol
SU864005310A SU1430366A1 (en) 1986-01-09 1986-01-09 Method of biological purifying of high-concentration waste water

Publications (3)

Publication Number Publication Date
GB8700233D0 GB8700233D0 (en) 1987-02-11
GB2187181A true GB2187181A (en) 1987-09-03
GB2187181B GB2187181B (en) 1990-05-23

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Family Applications (1)

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GB8700233A Expired - Fee Related GB2187181B (en) 1986-01-09 1987-01-07 Purification of sewage

Country Status (5)

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CN (1) CN87100664A (en)
DE (1) DE3700503A1 (en)
FR (1) FR2595683B1 (en)
GB (1) GB2187181B (en)
IT (1) IT1216773B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110184223B (en) * 2019-06-17 2020-11-24 华中农业大学 Arthrobacter calsium capable of removing ammonia nitrogen in culture sewage and application thereof
CN112939208A (en) * 2021-02-01 2021-06-11 山西沃能化工科技有限公司 Activated sludge culture method suitable for ethylene glycol wastewater biochemical treatment

Also Published As

Publication number Publication date
GB2187181B (en) 1990-05-23
IT1216773B (en) 1990-03-14
FR2595683B1 (en) 1988-10-21
CN87100664A (en) 1987-08-26
GB8700233D0 (en) 1987-02-11
FR2595683A1 (en) 1987-09-18
DE3700503A1 (en) 1987-07-16
IT8747508A0 (en) 1987-01-08

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PCNP Patent ceased through non-payment of renewal fee