GB2050836A - A preparation or combating noxious arthropods in horticultural produce - Google Patents

A preparation or combating noxious arthropods in horticultural produce Download PDF

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Publication number
GB2050836A
GB2050836A GB8015866A GB8015866A GB2050836A GB 2050836 A GB2050836 A GB 2050836A GB 8015866 A GB8015866 A GB 8015866A GB 8015866 A GB8015866 A GB 8015866A GB 2050836 A GB2050836 A GB 2050836A
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prey
mites
preparation
preparation according
light material
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GB2050836B (en
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Koppert P A
Koppert P C
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Koppert P A
Koppert P C
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • A01N63/16Arachnids

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  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Agronomy & Crop Science (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Insects & Arthropods (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)

Abstract

A preparation for combating noxious arthropods in horticultural produce comprising a spreadable mixture of living mites of prey and a granular light material. A method for producing the preparations and for using the preparations is also disclosed.

Description

SPECIFICATION A preparation for combating noxious arthropods in horticultural produce The invention relates to a preparation for combating noxious arthropods in horticultural produce and to a method forthe preparation and application of such a preparation.
It is common knowledge that Tetranychidae in vegetable, fruit and flower plants may be combated by biological process by making use of their natural enemies, more particularly mites of prey. Generally a plant-leaf is laid on the plants infested by Tetranychidae, on which leaf there is a number of specimens of a mite of prey. The mites of prey pass to the leaves of the plant and hunt their prey in the form of the Tetranychidae. They then feed on these and propagate in the meantime to supplying a new generation of mites of prey which will also live at the cost of the prey. In his manner, the infestation of the plants by Tetranychidae is arrested or reduced to a negligible minimum after some time. The mites of prey are not noxious to the plants and are not annoying to man because, after performing their task, they die out naturally.The method yields excellent results, particularly when applied to vegetable and flower plants.
In the said method use is made of mites of prey which are on a plant-leaf. This gives rise to problems as regards the storage, dosing and processing of the living material. The mites of prey normally have to be specifically bred on the plant-leaf concerned, which is mostly done in a separate breeding. At a certain time, when the mites of prey are in a mobile phase of their development, the plant-leaf must be reaped. Since such a plant-leaf is an unstable material and can be kept for at most a few days it has to be used for example, in a market-garden within this time-limit or otherwise its usefulness is destroyed. In the application there arises the problem of how to spread the slack material on the infested plants, while spreading the plant-leaves on the plants is relatively labour intensive.
It is the object of the invention to meet these problems by providing the living material for the biological combating in the form of a preparation which may be kept for a longer period, the dosing of which is easier and which may be spread over the infested plants with less trouble.
This invention is based on the observation that living mites of prey, for instance of the genus Phytoseiuluspersimilus, may easily be mixed with granular light materials to form a scatterable or dispersable mixture. Under conditions of temperature, light and humidity in which the mites of prey are practically inactive, they will remain packed up between the grains of the granular light material, so that the mixture may be preserved and packed as a stable preparation. Moreover, if some food is added to the mixture, a relatively long period of preservation of the preparation is possible. The preparation may be made up in any dilution desired and be divided into portions of any size desired, which allows good dosing. Moreover, the preparation may be applied to the infested plants by spreading, in other words, by scattering or dispersing.After application of the preparation to the plants, the mites of prey will emerge from the preparation under the influence of the prevailing conditions (temperature, light and humidity) and pass to the plants where they may go in search of the arthropods present there, which are their prey, and combat them in the manner indicated above. The packeted preparation does not pose a threat to the surroundings and, in suitable conditions, it may be kept for a reasonably long time.
Thus, the invention provides a preparation for combating noxious arthropods in horticultural produce, the preparation comprising a scatterable mixture of living mites of prey and a granular light material. Furthermore, the invention provides a method of producing such a preparation and a method for the application thereof.
For the preparation according to the invention, living mites of prey are particularly suitable because, being arachnids they mix well with the granular light material and, if kept under cool conditions, form a stable mixture. A good example is the mite of prey of the genus Phytoseiulus persimilis which may serve for combating tetranychidae of the genus Tetranychus urticae. However, numerous other mites of prey, among which mites of prey which may be launched against insects or other arthropods, are also useful.
The mites of prey to be processed in the preparation need not be adult but may be in any stage of development (eggs, larvae, nymphs, images) since in all these stages of development they may be mixed with the granular light material. Often various stages of development will occur side by side. With the exception of the eggs, all these stages of development are immediately useful for combating noxious arthropods in horticultural produce. In this specification the term "mites of prey", includes all the stages of development unless the alternative is stated.
For the other component of the mixture any type of granular light material may be used. the material must be granular and should also be lightweight so as to ensure good spreadability (i.e. scattering or dispersing) of the mixture. It should, of course, also be non-toxic to mites of prey and non-phytotoxic and not lead to environmental problems. The dimensions of the grains may be adapted somewhat to those of the mites of prey and be, for instance, 1 to 5 times as large as the mites of prey; since the dimensions of the mites of prey may diverge, this means that the dimensions of the grains may vary from, for instance, 0.1 mm to, for instance, 4 mm.
Also the density of the grains may be adapted to that of the mites of prey and amount to, for instance, 1 to 5 times that of the mites of prey. The shape of the grains is less important, but preferably rectangular or angular grains are used, because round grains spread on a plant will easily roll off the leaves.
Further, the grains may be solid or porous, as desired.
As a granular light material particularly sawdust, which mixes well with the mites of prey to form a scatterable and non-perishabie mixture and does not cause problems when spread over the plants has given satisfaction. Also useful are:ground polystyrene foam, broken hollow thermoplastic resin pearls, porous cellulose derivatives and expanded grains of perlite or vermiculite.
The mixture ratio between mites of prey and granular material is not critical, which means that any desired dilution of the mites of prey is possible.
Of course, so much granular material should be provided that the preparation may be dosed well, in other words, that it is easily divided into portions of the desired size. In one particular embodiment, an amount of about 2000 mites of prey to about 200 ml of saw-dust has given satisfaction, but larger or smaller amounts of granular material are also possible.
Sometimes it may be to advantage to introduce into the preparation some food for the mites of prey as a third component. This food preferably comprises living prey animals (Tetranychidae, for instance), but known vegetable foods, such as pollen or honey, may be used. The food may serve for the preservation of the preparation since the mites of prey can live therein without food for a limited time only. It is, however, an important aspect of the addition of food that later on, during the application of the preparation, the mites of prey may be helped through a difficult period.
When the preparation has been scattered over a plant, the mites of prey emerge from it and go in search of their prey in the form of Tetranychidae or other anthropods which have infested the plant; the food provided in the preparation may then be helpful to keep the mites of prey alive for so long as they have not found this prey. Besides, the addition of food in the preparation may help to lenghten the time of action of the preparation, since in this manner the mites of prey keep alive longer and are capable of suppressing further infestation, if any, by noxious anthropods.
Dependent on the object in view, the amount of food in the preparation may be larger or smaller; for instance, in actual practice an amount of 1 to 20 prey animals per mite of prey has been found to be useful for a long time of preservation and for bridging the transition period If vegetable foodstuffs are used as a foodcomponent, the granular light material should be well compatible with these foodstuffs and not cake under the influence thereof. In this case, saw-dust has been found to be especially suitable as the granular light material.
The invention also provides a method of producing the said preparation which consists in that the living mites of prey are mixed with a granular light material in a desired proportion. For this purpose it is necessary to breed the mites of prey first.
The breeding of the mites of prey may be done in any suitable manner, for instance, on plant-leaves, with prey animals serving as food. In favourable conditions of temperature, humidity and food, a regular development takes place, after which the mites of prey can be reaped. Dependent on the manner and time of reaping, the mites of prey may be obtained in one sigle stage of development (images, for instance) or in a combination of stages of development (for instance) larvae, nymphs and images with possibly still some eggs between them).
As stated hereinbefore, all these stages of development are useful and they are not distinguished from each other hereafter.
As soon as a suitable amount of mites of prey has been collected, these mites of prey are mixed with a suitable amount of granular light material and, if necessary, some food so as to obtain the preparation according to the invention. The mixing of the mites of prey may be done by simply bringing together these components, and usually shaking or stirring lightly is sufficient to bring about a good mixture.
Food in dry condition, such as pollen or anima prey, may be added at the same time and in the same manner, but in the case of fluid food, such as fluid honey, a method should be chosen in which sticking together of the mites of prey is avoided. Preferably the fluid food is first mixed with the granular light material (for instance by spraying or stiffing) till the food has been well absorbed by the granular material, after which the mites of prey are admixed in the described manner.
At 10-13"C the preparation obtained is stable enough. At a lower temperature, for instance 5-1 0 C, the stability becomes even higher because the mobility of the mites of prey is then strongly reduced. It is, therefore, to advantage to keep the preparation in cooled condition. Furthermore, if animal prey, such as Tetranychidae, is present in the preparation as food, it is also of advantage to keep the preparation in the dark, since Tetranychidae remain inactive at lower temperatures and in the absence of light. In addition, the stability and the keeping qualities of the mixture are dependent on the degree of humidity in the atmosphere, so that preferably also the humidity is maintained at a favourable high value.Taking into consideration all these conditions, the preparation may be kept for a long time; in actual practice it has been found that a preparation of mites of prey with saw-dust and added Tetranychidae at a humidity of 90-95% R.H.
can be kept in the dark for even 2-3 weeks.
The preparation may be kept in a suitable container and, with a view to the dosing, it may be divided on a number of smaller containers, if desired. For these containers a dredging-box or dredging-cup or a box provided with a dredging lip may be used to advantage, so that the preparation may later on be spread directly on the plants. Alternatively a normally closed container from which the preparation may later on be delivered to a horticultural dispenser or any other common dispenser, is useful. As a material for the containers, preference is given to a plastics material orto a plasticlined material, because the moisture content in the closed container may then be maintained. The said containers are also useful for the despatch of the preparation to the place of application, but if desired an additional packing may be arranged around the container.
In the place of application (for instance a marketgarden) the package may be opened and the preparation may be spread by spraying or dispersing over plants infested by Tetranychidae or other arthropods. The preparation remains on the leaves of the plants for the greater part and only a small portion of it falls on the ground. Under the influence of the prevailing conditions of temperature, light and humidity, activation of the living material in the preparation takes place, in which the mites of prey (or at least the mobile stages of development thereof) emerge from the preparation and pass to the leaves of the plants. At first, the mites of prey still feed on the available food, if any, in the preparation and then go in search of prey on the plants in the form of arthropods which cause the infestation. As soon as the mites of prey have found their prey, they feed on it.They also propagate for supplying new generations of mites of prey which then live on the noxious arthropods. As a result thereof, the infestation of the plants by arthropods will be suppressed or reduced to a minimum after some time. After performing their duty, the mites of prey will die out in the natural way.
Thanks to the composition of the preparation, excellent dosing is possible. The right dose depends on the degree of infestation of the plants by arthropods and can easily be determined by experience.
The following Examples illustrate the invention.
Example 1 A mite of prey (Phytoseiulus persimilis) is allowed to lay its eggs on a beanleaf and the eggs and larvae produced are bred further on this leaf. After 5 to 7 days, the mites of prey produced are reaped by brushing, all the stages of development present (larvae, nymphs, images with some eggs, if any) off the leaf and retained. The greater part of the mites of prey caught is kept in a refrigerator at 5-10"C, while a small portion fed with, for instance, Tetranychus articae, is used for the propagation and supply of new generations of mites of prey.
As soon as a sufficient amount of mites of prey has been reaped, these are mixed with saw-dust and an adequate amount of food in the form of living Tetranychidae. By light shaking a close mixture is obtained which is then divided into a number of plastic cups with dredging-lip. Per cup of about 300 ml a mixture of about 200 ml of saw-dust with about 2000 mites of prey and about 2000 Tetranychidae is provided.
The dredging-cup with its content is kept in a refrigeratorforsome days at 5-10"C and a degree of humidity of 90-95% R.H. (possible keeping time about 3 weeks), then may be packed in a cardboard box and despatched to a market-garden. If the distance of travel is long, cooling should be applied while in transit, for short distances this is not necessary. Thanks to the relatively low temperature of the air prevailing in the cup and the nontransparent package, the preparation remains stable.
This preparation is sufficient for application over an area of about 500 m2 with a slight infestation by Tetranychidae.
Upon arrival at a market-garden, the package is opened and the preparation is spread from the dispenser over produce infested by Tetranychidae (for instance Tetranychus articae). The grains of the preparation remain for the greater part on the leaves of the plants. Under the influence of the prevailing temperature and the light present, the mites of prey (with the exception of the eggs) then become active and pass onto the leaves of the plants in search of their prey in the form of the Tetranychidae which have caused the infestation. Until they find this prey (for instance, due to uneven distribution of the Tetranychidae on the plants), the mites of prey can feed on the Tetranychidae still present in the preparation which, under the prevailing conditions of temperature and light, also becomes active. Thereafter, they feed on the Tetranychidae which were already present on the plants.Also they propagate by laying eggs on the back of the leaves from which new generations of mites of prey emerge which also live on the Tetranychidae. The result is that the infestation of the plants is suppressed or stopped after a short time. Thereafter the mites of prey die out in natural way.
Example Il The procedure of example I is repeated with the difference that this time, when the preparation is composed, no Tetranychidae are added. The preparation obtained will keep for some days and gives good results when spread over plants infested by Tetranychidae, but shortly after spreading there occurs a certain mortality among the emergent mites of prey if these mites do not find prey immediately.
Example Ill The procedure of example I is repeated with the difference that the preparation is now kept in a larger plastic container and later spread over the infested plants by means of a horticultural dispenser. The results are the same as those of example I.
Example lV The procedure of example I is repeated with the difference that the mites of prey are mixed with the grains of ground polystyrene foam (about 1 grain per mite of prey) and with Tetranychidae. The total amount of mixture per cup is about 200 ml. This preparation is kept in the same manner and packed and despatched in the same manner as in example I.
When spread over plants which are lightly infested by Tetranychidae, similar results are obtained, but less grains remain on the leaves of the plants.

Claims (25)

1. A preparation for combating noxious arthropods in horticultural produce, comprising a spreadable mixture of living mites of prey (as hereinbefore defined) and a granular light material.
2. A preparation according to claim 1, characterized in that the mites of prey are of the genus Phytoseiulus persimilis.
3. A preparation according to claim 1 or 2, characterized in that the mites of prey are in the form of eggs, larvae, nymphs and/or images.
4. A preparation according to claim 1, characterized in that the granular light material has grain dimensions from 0.1-4 mm.
5. A preparation according to claim 4, characterized in that the grains are rectangular or angular.
6. A preparation according to claim 4 or 5, characterized in that the granular light material consists of saw-dust.
7. A preparation according to claim 4 or 5, characterized in that the granular light material consists of ground polystyrene foam.
8. A preparation according to claims 1-7, characterized in that the mixture comprises about 2000 mites of prey in about 200 ml of granular light material.
9. A preparation according to claim 1, characterized in that also food for the mites of prey is included in the mixture.
10. A preparation according to claim 9, characterized in that the food comprises living prey animals, such as Tetranychidae.
11. A preparation according to claims 1-10, characterized in that the preparation comprises about 2000 mites of prey and about 2000 prey animals in about200 ml of granular material.
12. A preparation according to claims 1-11, char- acterized in that the preparation is in a dredging-box or dredging-cup.
13. Amethod of producing a preparation for combating noxious arthropods in horticultural produce, characterized in that living mites of prey are mixed with a granular light material and, if necessary, some food for the mites of prey, this forming a spreadable preparation.
14. A method according to claim 13, characterized in that living mites of prey are mixed with saw-dust and, if necessary, living preys.
15. A method according to claim 13 or 14, characterized in that the mixing is done by simply combining the components by shaking or stirring lightly.
16. A method according to claim 13 or 14, characterized in that first a fluid food is mixed with the granular light material, to be absorbed by that material andonlythereafterthe mites of prepare admixed.
17. A method according to claims 13-16, characterized in that the mixture obtained is kept at 5-10"C and a humidity of the air of 90-95% R.H.
18. A method according to claims 13-17, characterized in that the mixture, in which Tetranychidae are provided, is kept in the dark at 5-10"C and a humidity of the air of 90-95% R.H.
19. A method according to claims 13-18, characterized in that the mixture obtained is kept in a dredging-box or dredging-cup.
20. A method of combating noxious arthropods in horticultural produce, characterized in that a mixture of living mites of prey and a granular light material with, if necessary, some food for the mites of prey, is spread over plants infested by arthropods.
21. A method according to claim 20, characterized in that a preparation according to claims 1-12 or the product of the method of claims 13-19 is used thereof.
22. A method according to claim 20 or 21, characterised in that spreading is done by spraying or dispensing.
23. A preparation according to claim 1, substantially as described herein with reference to any one of the Examples.
24. A method as claimed in claim 13, substantially as described herein with reference to any one of the Examples.
25. A method as claimed in claim 20, substantially as described herein with reference to any one of the Examples.
GB8015866A 1979-05-14 1980-05-13 Preparation for combating noxious arthropods in horticultural produce Expired GB2050836B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NLAANVRAGE7903776,A NL176903C (en) 1979-05-14 1979-05-14 AGENT FOR COMBATING HARMFUL ARTISTS IN AGRICULTURAL AND GARDEN CROPS AND A METHOD FOR PREPARING SUCH AN AGENT.

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GB2050836A true GB2050836A (en) 1981-01-14
GB2050836B GB2050836B (en) 1983-03-02

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GB (1) GB2050836B (en)
NL (1) NL176903C (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0396412A1 (en) * 1989-05-04 1990-11-07 Biological Crop Protection Limited Pest control
EP0777963A1 (en) * 1995-12-04 1997-06-11 Sumitomo Chemical Company, Limited Method for rearing or transporting entomophagous insects
EP2910119A1 (en) * 2014-02-21 2015-08-26 Biobest Belgium NV Pollen compositions and uses thereof
WO2020025709A1 (en) * 2018-07-31 2020-02-06 Bioline France Biological control system comprising predator acarians in a case

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH666987A5 (en) * 1985-06-06 1988-09-15 Zuericher Beuteltuchfabrik Ag DEVICE FOR RECEIVING AND STORING USER ORGANISMS AND METHOD FOR THE PRODUCTION THEREOF.

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0396412A1 (en) * 1989-05-04 1990-11-07 Biological Crop Protection Limited Pest control
EP0777963A1 (en) * 1995-12-04 1997-06-11 Sumitomo Chemical Company, Limited Method for rearing or transporting entomophagous insects
US5784991A (en) * 1995-12-04 1998-07-28 Sumitomo Chemical Company, Limited Method for rearing or transporting entomophagous insect
EP2910119A1 (en) * 2014-02-21 2015-08-26 Biobest Belgium NV Pollen compositions and uses thereof
WO2015124704A1 (en) * 2014-02-21 2015-08-27 Biobest Belgium Nv Pollen compositions and uses thereof
WO2020025709A1 (en) * 2018-07-31 2020-02-06 Bioline France Biological control system comprising predator acarians in a case
FR3084562A1 (en) * 2018-07-31 2020-02-07 Bioline France BIOLOGICAL CONTROL SYSTEM COMPRISING PREDATORY MITES IN A CASE

Also Published As

Publication number Publication date
NL176903B (en) 1985-02-01
NL176903C (en) 1985-07-01
FR2456477A1 (en) 1980-12-12
NL7903776A (en) 1980-11-18
GB2050836B (en) 1983-03-02

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Effective date: 19930513