GB202205696D0 - Method for producing double stranded DNA - Google Patents

Method for producing double stranded DNA

Info

Publication number
GB202205696D0
GB202205696D0 GBGB2205696.4A GB202205696A GB202205696D0 GB 202205696 D0 GB202205696 D0 GB 202205696D0 GB 202205696 A GB202205696 A GB 202205696A GB 202205696 D0 GB202205696 D0 GB 202205696D0
Authority
GB
United Kingdom
Prior art keywords
stranded dna
double stranded
producing double
producing
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB2205696.4A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Moligo Technologies AB
Original Assignee
Moligo Technologies AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Moligo Technologies AB filed Critical Moligo Technologies AB
Priority to GBGB2205696.4A priority Critical patent/GB202205696D0/en
Publication of GB202205696D0 publication Critical patent/GB202205696D0/en
Priority to EP23721604.9A priority patent/EP4511502A2/en
Priority to US18/856,778 priority patent/US20250250598A1/en
Priority to JP2024561941A priority patent/JP2025512568A/ja
Priority to CN202380048388.XA priority patent/CN119421957A/zh
Priority to PCT/EP2023/060150 priority patent/WO2023203082A2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
    • C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
    • C12Y605/01001—DNA ligase (ATP) (6.5.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
GBGB2205696.4A 2022-04-19 2022-04-19 Method for producing double stranded DNA Ceased GB202205696D0 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
GBGB2205696.4A GB202205696D0 (en) 2022-04-19 2022-04-19 Method for producing double stranded DNA
EP23721604.9A EP4511502A2 (en) 2022-04-19 2023-04-19 Method for producing double stranded dna
US18/856,778 US20250250598A1 (en) 2022-04-19 2023-04-19 Method for producing double stranded dna
JP2024561941A JP2025512568A (ja) 2022-04-19 2023-04-19 二本鎖ｄｎａを生成するための方法
CN202380048388.XA CN119421957A (zh) 2022-04-19 2023-04-19 生产双链dna的方法
PCT/EP2023/060150 WO2023203082A2 (en) 2022-04-19 2023-04-19 Method for producing double stranded dna

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB2205696.4A GB202205696D0 (en) 2022-04-19 2022-04-19 Method for producing double stranded DNA

Publications (1)

Publication Number Publication Date
GB202205696D0 true GB202205696D0 (en) 2022-06-01

Family

ID=81753228

Family Applications (1)

Application Number Title Priority Date Filing Date
GBGB2205696.4A Ceased GB202205696D0 (en) 2022-04-19 2022-04-19 Method for producing double stranded DNA

Country Status (6)

Country Link
US (1) US20250250598A1 (enExample)
EP (1) EP4511502A2 (enExample)
JP (1) JP2025512568A (enExample)
CN (1) CN119421957A (enExample)
GB (1) GB202205696D0 (enExample)
WO (1) WO2023203082A2 (enExample)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120156731A1 (en) * 2007-03-23 2012-06-21 The Board Of Trustees Of The Leland Stanford Junior University Improved Methods for Rapid Gene Synthesis
KR102160389B1 (ko) * 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
US20190322998A1 (en) * 2017-04-27 2019-10-24 University Of Southampton One pot assembly
GB201901583D0 (en) 2019-02-05 2019-03-27 Ducani Cosimo Method and products for producing funtionaslised single stranded oligonucleotides
GB202002547D0 (en) 2020-02-24 2020-04-08 Moligo Tech Ab Method and products for producing single stranded DNA polynucleotides

Also Published As

Publication number Publication date
CN119421957A (zh) 2025-02-11
US20250250598A1 (en) 2025-08-07
JP2025512568A (ja) 2025-04-17
WO2023203082A3 (en) 2023-12-07
WO2023203082A2 (en) 2023-10-26
EP4511502A2 (en) 2025-02-26

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)