GB1576585A - Chromatographic analysis of a liquid sample - Google Patents
Chromatographic analysis of a liquid sample Download PDFInfo
- Publication number
- GB1576585A GB1576585A GB1014778A GB1014778A GB1576585A GB 1576585 A GB1576585 A GB 1576585A GB 1014778 A GB1014778 A GB 1014778A GB 1014778 A GB1014778 A GB 1014778A GB 1576585 A GB1576585 A GB 1576585A
- Authority
- GB
- United Kingdom
- Prior art keywords
- solvent
- vessel
- sample
- plug
- mass spectrometer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0431—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
- G01N30/724—Nebulising, aerosol formation or ionisation
- G01N30/7253—Nebulising, aerosol formation or ionisation by thermal means, e.g. thermospray
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
- G01N30/7273—Desolvation chambers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7293—Velocity or momentum separators
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Description
(54) CHROMATOGRAPHIC ANALYSIS OF A LIQUID SAMPLE (71) 1-, ERIK RAGNAR RYHAGE, a
Swedish Subject, of Armfeltsgatan 5, 115 34
Stockholm, Sweden, do hereby declare the invention, for which I pray that a patent may be granted to me, and the method by which it is to be performed, to be particularly described' in and by the following statement: This invention relates to the analysis of a liquid' sample consisting of a plurality of components by using a liquid chromatograph and a mass spectrometer or mass spectrograph.
According to one aspect of the present invention, there is provided a method of analysing a liquid sample consisting of a plurality of components, which method comprises:
(a) dissolving the sample in a solvent; (b) supplying the resultant solution to a liquid chromatograph so as to resolve the components of the sample;
'(c) eluting the resolved components of the sample from the liquid chromatograph and passing at'least part of the eluted solution into a vessel;
(d) evaporating at least part of the eluting solvent from the solution in the vessel and evacuating the evaporated solvent from the vessel; and
(e) supplying the remaining sample and solvent' (if any) to a mass spectrometer or mass spectrograph.
According to another aspect of the present invention, there is provided apparatus for analysing a liquid sample consisting of a plurality of components, which apparatus comprises a liquid chromatograph, a mass
spectrometer or mass spectrograph, and means for supplying the liquid sample in the form of a succession of resolved components eluted from the liquid chromatograph to the mass spectrometer or mass spectrograph. which means incorporates:
(i) a vessel' for receiving the sample dissolved in the eluting solvent; and (a) a plug through which the sample dissolved in the solvent can pass and so disposed in-the vessel that, in use, the sample must pass through the plug before reaching the- mass spectrometer or spectrograph.
According to yet another; aspect of the present invention there is provided a method of analysing a liquid sample consisting of a plurality of components using a 4quid chromatograph and a mass spectrometër or mass spectrograph, in which method the sample is introduced into the liquid chromatograph dissolved in a solvent, the resolved sample is eluted from the liquid chromatograph into a heated vessel containing- a heated plug through which it passes, the output of which vessel is in communication with the ion source of the mass spectrometer or mass. spectrograph, the pres- sure and temperature within the vessel and plug being such that at least part of the eluting solvent is evaporated within. the vessel or plug, the evaporated solvent is evacuated from the vessel, and the sample and remaining solvent (if any} is supplied to the ion source of the mass spectrometer or mass spectrograph.
Chromatography is a method of separating the components of a mixture of organic compounds which is based on the principle that different components become bound to different degrees to a "stationary phase", which can be either solid or liquid, the components being removed from the stationary phase by means of a "mobile" phase" which can be a liquid (liquid chromatography) or a gas (gas chromatography3. In chromatography, e.g. liquid chromatography, an automatic fraction collector is usually used for separately collecting the components eluted.Those automatic fraction collectors which are available operate according to different principles; for example they separate the fractions with respect to time, by measuring volume by counting drops or by weighing. Each fraction can then be analysed. Continuous monitoring of the composition of the eluate can be achieved in various ways. High sensitivity is obtained byf using an interferometric. method which is based on light deviation. in a- double prism one half of which comprises the soivent ahd the other half of which contains' the
eluate.Another method is based on the
measuring of the UV-absorption of the eluate. The UV absorption of the eluate is determined in a' through-flow cuvette. The
'sensitivity can be about 10 mg/ml for
'certain. organic compounds. By allowing the
components to condense on an endless wire
,which passes, through a flame ionisation detector at a certain velocity, the sensitivity can be increased to about 1 mg/ml of the
components. -
A mass spectrometer or mass spectro
graph is a device which produces ions and
is then capable of separating the ions accord
ing to their mass/charge ratio, i.e. according
to. their specific mass.A sample to be analysed is ionised in an ionisation cham b.er, e:g. through electron impact, possibly
after having been heated, and the resulting
ions are accelerated by means of an electric
field from the ion source towards the
analyser. In the analyser the ions pass.
through a magnetic or electric field which
separates the ions in accordance with their
specific mass. Ions having different specific
masses will pass through the analyser along
different paths, and are detected in a manner
known per Se. Each ion beam correspond
ing to ..ions of a given specific mass can be
collected and discharged.
Methods known per se for analysing the
composition of the eluate in liquid chromato
graphy comprise allowing the components
to condense on an endless wire which 'passes through an ionisation chamber of the mass
spectrometer at a certain velocity, and' heat;
ing the wire so that the components are
evaporated and ionised (see, for example,
R. P. V. Scott, J. G. Lorens; J. Chrom, Sci: 8,1970, 65). German Patent Specification
No. 2424085 describes a similar method in
which the eluate is transferred to an endless
wire where it is heated for evaporation of
solvent before the sample is transferred to a
mass spectrometer.Another method is based
on the principle of using a spa'ill fraction of
the eluate from the - liquid chromatograph
which is' continuously supplied directly to
the ioriisation chamber of the mass spectro
meter where both the solvent and the sample
components are evaporated an ionised. U.S.
Patent Specification No. 3,997,298 discloses
a method in which a fraction of the eluate
is continuously fed into the ion source of
the mass - spectrometer. In this case how ever, fthere is - no concentration of sample
with respect to solvent before the sample is sup' plied' to the ion-source.
In border that the present invention may
be ,more fully understood,. three embodi menus of apparatus according to ,,the present
invention. will now be .described, .by way of e'xainple,, ',with reference to .the - accom- , panying drawings, in which: Figures 1 to 3 each schematically shows
a respective one of these embodiments.
In Figures 1 reference numeral 1 denotes
a liquid. chromatography column which is supplied with a mobile phase from a vessel 22 via a pump 20. A sample consisting of a
plurality of components is injected into the
column 1 in a conventional manner by means of a syringe 19. The sample is dis
solved in the mobile phase. The-components of the sample separated in the column 1
and the solvent pass to a splitter.2 from
which one part of the eluate is passed .through a UV-detector 3 to a vessel 21 and
another part of the eluate is introduced into
a heated vessel 4 in drops 5.The drops fall
On a heated body in the form of a porous
plug 6 which can, for example, consist of
the packing material used in gas chromato
graphy columns SE-30.
A carrier gas, e.g. helium, is introduced
into the vessel 4 from a vessel 17 via a con
duit 23 so as to obtain a higher pressure
above the plug 6 than below the plug 6. In the lower part of the vessel 4 the pressure
is considerably below atmospheric pressure, since this part of the vessel 4 is connected to a vaccum duct 8 provided with a pump
(not shown) via a valve 9. The lower part of
the vessel 4 is also in communication with
an ion source 10 of a mass spectrometer
via a molecule separator -11. The valve 9 is self monitoring and this end comprises an electromagnet 12 which opens the. valve more the higher -the pressure in the lower part of the vessel.By changing the current
in the coil of the electromagnet, it is possible
to alter the force which is required to close
the valve so as to increase or. decrease the
flow to the ion source 10.
By supplying heat to the drops 5 by means
6f a heater 13 a portion of the solvent is
evaporated so that the concentration of
the sample is increased. As each drop 5 passes through the lug 6, the pressure
will increase in the lower part of the vessel
4, thus causing the valve 9 to open and a
substantial part of the vapour of the solvent to be evacuated. This reduces the pressure
and causes the valve 9 to close again and the
somewhat delayed sample (in vapour form)
passes together with the carrier gas and
residual solvent vapour to the molecule
separator where the greater part of carrier
gas is evacuated, and then to the ionisation
chamber 14 of the ion source 10, said ion source having pressure of between 5 and 1010, mm Hg.
In Figure 2, in which those parts corre
sponding to like parts in Figure 1 have the
same reference numerals, the ion source 10
is directly connected to the plug 6 which.
in this case may consist of sintered glass.
.By supplying heat to each drop 5, as the
drop is increasing in size, ? considerable proportion of the solvent can be evaporated,
thus increasing the sample concentration.
As soon as the drop has fallen onto the
heated sintered glass, the solvent is evapor
ated due to its high vapour pressure which
gives rise to an overpressure in the vessel 4
as compared to the pressure in the ionisation
chamber 14. This pressure is also increased
by supplying the carrier gas from the vessel
17 to the vessel 4. Because of the tempera ture in the vessel 4 and the temperature of
the sintered glass the sample passes through
the sintered glass and reaches the ionisation
chamber 14 somewhat delayed as compared
to the solvent.
The overpressure which is generated in
the ionisation chamber when the solvent is
evaporated causes the valve 9 which is con
nected to the vacuum duct 8 to open for a
short time until- the pressure has been re
duced to an acceptable level for ionising the
sample which occurs in- conventional man
ner, e.g. by electron impact.
In Figure 3,which shows- a third embodi
ment, like parts have the same reference
numerals as in the previous figures. The
splitter 2 is connected to the vessel 4 by
means of a tube 7 having a very small in
ternal diameter. The length of tube 7 which
extends into the vessel 4 through a seal
may be varied. The vessel 4 is maintained
under low pressure by means of a vacuum
Pump 27 and a duct 26. The vessel 4 may
be connected to the pump 27 via a 'cooling device 25.The lower part of the vessel
4 is in direct communication with the
ionisation source 10 and ionisation cham
ber 14 of a conventional mass spectro
meter via the plug 6 which could be
provided with one or more bores.or could
be made of a porous material made
of metal, glass or ceramics for ex
ample. Depending on the composition of
the eluate, the plug 6 could be provided
with bores having a diameter of between l0-3and 0.5 mm. When the eluatè from the
column 1 meets the plug 6 a substantial
proportion of the solvent is evaporated and
evacuated so as to increase the sample con
centration which has a lower vapour pres
sure than the solvent. The sample and the
residual solvent are introduced into the
ionisation chamber 14.The vessel is main
tained under low pressure by the vacuum
pump 27 so as to obtain - a inore efficient
evacuation of solvent. The temperature and
pressure of the vessel 4 are varied in
accordance with the concentration and solu
bility of the components to' be analysed.
The porosity and temperature of the plug
6 should be'adapted to the solvent used:
The major portion of the sample which has
a lower vapour pressure -than the solvent
will pass through the plug 6 since the ionisation chamber has a lower pressure than the lower 'part of the vessel 4. Depending on the components to be analysed, it could be advantageous to supply additional gas or solvent to the lower part of the vessel. This supply is made from the vessel 17 via the duct 23. This gas or solvent will increase the pressure in the vessel 4 art the same time as the gas passing through the porous plug 6 increases the pressure in the ionisation chamber. If the pressure in.
the ionisation chamber is between 0.01 and 5 mm Hg an ionisation method called chemical ionisation is advantageously utilized. Chemical ionisation is an ionization method in which a reaction gas, e.g. methane, is ionised by means of electrons and the resultant molecular or fragmentary ions transfer protons and electrons to the mole- cules of the substance to be analysed. The solvent of the eluate, e.g. hexane, could act' as the reaction gas. However further gas could also be supplied either by way of the porous plug 6 or directly to the ionization chamber.
A large variation in the amount of the eluate per hour could be permitted, e.g. a variation between 0.06 and 60 ml/h. The heater for the vessel 4 can be, for example, a current-carrying element, an induction heater or a radiation emitter, e.g. a laser.
In order to protect the filament 16 of the ion source from oxidation and destruction caused by residual solvent vapour, such as, for example, water vapour, or methanol, hexane or certain reaction gases produced during chemical ionisaflon such as isobutane, oxygen etc., a safety gas may be supplied to the filament so that a minimum of residual vapour or gas or reaction gas is in contact with the filament. This may be achieved by supplying the safety gas, e.g.
helium, to the ionisation chamber from a vessel 18 via a duct 15, the mouth of the duct 15 being located in the ionisation chamber very close to the input bore for electrons from the filament. Since there is a higher pressure in the ionisation chamber than in the filament chamber a beam of helium will thus be incident on the filament and protect the filament from solvent vapour.
If the eluate from the liquid chromatograph is transferred to the mass spectrometer as described above the following advantages are obtained:
(a) By heating the vessel 4 to a suitable temperature, a high proportion'of the solvent can be evaporated and evacuated whereas the sample passes through the porous plug together with the residual solvent and is introduced in to the ionisation chamber 14; (b) By supplying additional gas, such as helium; or -additional solvent to the' vessel 4 the under pressure in the vessel 4 and thus -the flow-can be -modified so as to positively affect the separation of the sample from the solvent;
(c) It is possible to detect both positive and negative ions by means of chemical ionisation and the pressure in the ionisation chamber 14 of the mass spectrometer can be controlled by the flow from the column the temperature in the vessel 4 andthe porous plug 6, as well as by supplying additional solvent or gas; (d) An - identification of the molecular weight and fragmentation of the separated organic components and thus their structure is possible;
(e) It is possible to detect amounts - of components in the nanogram range::
(f) It is possible to analyse material at very low vapour pressure; and
(g) It is possible to analyse temperature unstable' molecules.
The methods described above with refer pence to the figures combine liquid chromatography, e.g. column high pressure liquid chromatography, with mass spectrometery and ensure that the sample to solvent ratio of the eluate of the liquid chromatograph is substantially increased before the sample is introduced- into the mass spectrometer.
WHAT I CLAIM IS: 1. A method of analysing a liquid sample consisting of a plurality of components, which method comprises: (a) dissolving the sample in a solvent;
(b) supplying the resultant solution to a liquid chromatograph - so as to resolve the components of the sample;
(c) eluting the resolved' components of the sample from the liquid chromatograph and passing at least part of the eluted solution into a vessel;
(d) evaporating at least part of the eluting solvent from the solution in the vessel and evacuating the evaporated solvent from the vessel; and
(e) supplying the remaining sample and solvent. (if any) to a mass spectrometer or mass spectrograph,
2. A method according to claim 1 wherein the evaporation of at least part of the solvent is achieved by lowering the pressure of the solution and/or by raising the temperature of the solution.
3. A method according to claim 1 or 2.
wherein the sample and solvent are passed through a plug within the vessel prior to being supplied to the mass spectrometer or mass spectrograph.
4. A method according to claim 3, wherein the plug is heated so as to cause at least part of - the solvent to evaporate as it nasses through the plug.
5. A method according to claim 3- or 4 wherein the sample and solvent (if anv) nassing out of the plug is; supplied' directly to the ion source of the mass spectrometer or mass spectrograph.
6. A method according to any of claims 3 to 5, wherein the plug is provided with bores so that the sample and solvent pass through the plug.
7. A method according to any of claims' 3 to 5, wherein the plug is a porous plug so that the sample and solvent pass through the plug.
8. A method according to claim 7, wherein the porous plug is made of glass.
9. A method according to claim 7, wherein the porous plug is made of a ceramic material.
10. A method according to claim 7, wherein the porous plug is made of metal.
11. A method according to, any preceding claim, wherein the vessel is heated.
-12. A method according to any preceding claim, wherein the solution is supplied to the vessel in drops, the drops passing through an atmosphere at such a temperature and such a pressure that solvent is evaporated from the drops.
13. 'A method according to any preceding claim, wherein the pressure increase generated in the vessel due to the evaporation of solvent serves to open a valve for evacuation of evaporated solvent.
14. A method according to any preced-' - ing claim, wherein evaporated solvent is evacuated from the, vessel by a pump.
15. A method according to any preceding claim, wherein additional solvent is supplied to the vessel to improve ionization in the mass spectrometer or mass spectrograph.
16. A method according to any preceding claim, wherein a carrier gas is supplied to the vessel so as to increase the pressure in the vessel.
17. A method according to claim 16, wherein the carrier gas serves to protect a filament of the ion source of the mass spectrometer or mass spectrograph from harmful gas or vapour.
18. A method according to any preceding claim, wherein a gas for protecting a filament of the ion source of' the mass spectrometer or spectrograph from harmful gas or vapour is supplied directly to the, ionization chamber of the mass spectrometer or mass spectrograph.
19. A method according to any preceding claim, wherein a further part of solution eluted from the liquid' chromatograph is paused into a UV-detector.
20. A- method according to any preceding claim (except claim S or any claim as dependent upon claim 5), wherein the sample and solvent (if any) supplied to the mass spectrometer or mass spectrograph passes through a molecule separator after leaving the vessel.
**WARNING** end of DESC field may overlap start of CLMS **.
Claims (27)
1. A method of analysing a liquid sample consisting of a plurality of components, which method comprises: (a) dissolving the sample in a solvent;
(b) supplying the resultant solution to a liquid chromatograph - so as to resolve the components of the sample;
(c) eluting the resolved' components of the sample from the liquid chromatograph and passing at least part of the eluted solution into a vessel;
(d) evaporating at least part of the eluting solvent from the solution in the vessel and evacuating the evaporated solvent from the vessel; and
(e) supplying the remaining sample and solvent. (if any) to a mass spectrometer or mass spectrograph,
2. A method according to claim 1 wherein the evaporation of at least part of the solvent is achieved by lowering the pressure of the solution and/or by raising the temperature of the solution.
3. A method according to claim 1 or 2.
wherein the sample and solvent are passed through a plug within the vessel prior to being supplied to the mass spectrometer or mass spectrograph.
4. A method according to claim 3, wherein the plug is heated so as to cause at least part of - the solvent to evaporate as it nasses through the plug.
5. A method according to claim 3- or 4 wherein the sample and solvent (if anv) nassing out of the plug is; supplied' directly to the ion source of the mass spectrometer or mass spectrograph.
6. A method according to any of claims 3 to 5, wherein the plug is provided with bores so that the sample and solvent pass through the plug.
7. A method according to any of claims' 3 to 5, wherein the plug is a porous plug so that the sample and solvent pass through the plug.
8. A method according to claim 7, wherein the porous plug is made of glass.
9. A method according to claim 7, wherein the porous plug is made of a ceramic material.
10. A method according to claim 7, wherein the porous plug is made of metal.
11. A method according to, any preceding claim, wherein the vessel is heated.
-
12. A method according to any preceding claim, wherein the solution is supplied to the vessel in drops, the drops passing through an atmosphere at such a temperature and such a pressure that solvent is evaporated from the drops.
13. 'A method according to any preceding claim, wherein the pressure increase generated in the vessel due to the evaporation of solvent serves to open a valve for evacuation of evaporated solvent.
14. A method according to any preced-' - ing claim, wherein evaporated solvent is evacuated from the, vessel by a pump.
15. A method according to any preceding claim, wherein additional solvent is supplied to the vessel to improve ionization in the mass spectrometer or mass spectrograph.
16. A method according to any preceding claim, wherein a carrier gas is supplied to the vessel so as to increase the pressure in the vessel.
17. A method according to claim 16, wherein the carrier gas serves to protect a filament of the ion source of the mass spectrometer or mass spectrograph from harmful gas or vapour.
18. A method according to any preceding claim, wherein a gas for protecting a filament of the ion source of' the mass spectrometer or spectrograph from harmful gas or vapour is supplied directly to the, ionization chamber of the mass spectrometer or mass spectrograph.
19. A method according to any preceding claim, wherein a further part of solution eluted from the liquid' chromatograph is paused into a UV-detector.
20. A- method according to any preceding claim (except claim S or any claim as dependent upon claim 5), wherein the sample and solvent (if any) supplied to the mass spectrometer or mass spectrograph passes through a molecule separator after leaving the vessel.
21. Apparatus for analysing a liquid
sample consisting of a plurality of components, which apparatus comprises a liqud chromatograph, a mass spectrometer or mass spectrograph, and means for supplying the liquid sample in the form of a succession of resolved components eluted from the liquid chromatograph to the mass spectrometer or mass spectrograph, which means incorporates:
(i) a vessel for receiving the sample dissolved in the eluting solvent; and
(ii) a plug through which the sample dissolved in the solvent can pass and so disposed in the vessel that, in use, the sample must pass through the plug before reaching the mass spectrometer or spectrograph.
22. Apparatus according to claim 21, wherein the vessel and/or plug are heatable.
23. Apparatus according to claim 21 or 22, wherein a valve is provided for evacuating evaporated solvent from the vessel.
24. Apparatus according to claim 21, 22 or 23, wherein means are provided for supplying a carrier gas to the vessel.
25. A method of analysing a liquid sample consisting of a plurality of components, which method is substantially as hereinbefore described. with. reference to any.
one of Figures 1 to 3 of the accompanying drawings.
26. Apparatus for anaylsing a liquid sample consisting of a plurality of components, which apparatus is substantially as hereinbefore described with reference to, and/or as illustrated in, any one of Figures 1 to 3 of the accompanying drawings.
27. A method of analysing a liquid sample consisting of a plurality of components using a liquid chromatograph and a mass spectrometer or mass spectrograph, in which method the sample is introduced into the liquid chromatograph dissolved in a solvent, the resolved sample is eluted from the liquid chromatograph into a heated vessel containing a heated plug through which it passes, the output of which vessel is in communication with the ion source of the mass spectrometer or mass spectrograph, the pressure and temperature within the vessel and plug being such that at least part of the eluting solvent is evaporated within the vessel or plug, the evaporated solvent is evacuated from the vessel, and the sample and remaining solvent (if any) is supplied to the ion source of the mass spectrometer or mass - spectrograph. -
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE7702900A SE415806B (en) | 1977-03-15 | 1977-03-15 | SET TO ANALYZE SOLUTIONS CONTAINING A NUMBER OF SUBSTANCES USING A LIQUID CHROMATOGRAPHER AND A MASS SPECTROMETER OR A MASS SPECTROGRAPHER AND DEVICE FOR IMPLEMENTATION OF THE SET |
| SE7802298A SE7802298L (en) | 1978-03-01 | 1978-03-01 | APPLY ACCORDING TO PATENT APPLICATION 7702900-7 THAT, USING A MASS SPECTROMETER RESPECTIVE A MASS SPECTROGRAPHER ASTADKOMMA A CONTINUOUS ANALYSIS OF A CHROMATOGRAPH DELIVERED ELUAT, AND A DEVICE FOR GENOMATOR |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1576585A true GB1576585A (en) | 1980-10-08 |
Family
ID=26656794
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1014778A Expired GB1576585A (en) | 1977-03-15 | 1978-03-14 | Chromatographic analysis of a liquid sample |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JPS5432391A (en) |
| DE (1) | DE2811300A1 (en) |
| GB (1) | GB1576585A (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5921502B2 (en) * | 1977-09-08 | 1984-05-21 | 日本分光工業株式会社 | How to introduce a sample from a liquid chromatograph to a mass spectrometer |
| DE3013620A1 (en) * | 1979-05-25 | 1980-12-04 | Hewlett Packard Co | SAMPLE INPUT DEVICE FOR MASS SPECTROMETER |
| JPS5987900A (en) * | 1982-11-11 | 1984-05-21 | 松下電器産業株式会社 | Electronic part mounting device |
| JPS59214485A (en) * | 1983-05-20 | 1984-12-04 | 三菱電機株式会社 | Automatic sewing machine |
-
1978
- 1978-03-14 GB GB1014778A patent/GB1576585A/en not_active Expired
- 1978-03-15 DE DE19782811300 patent/DE2811300A1/en active Pending
- 1978-03-15 JP JP3048178A patent/JPS5432391A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE2811300A1 (en) | 1978-10-05 |
| JPS5432391A (en) | 1979-03-09 |
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