GB1563299A - Methods of pregnancy testing and a test kit therefor - Google Patents
Methods of pregnancy testing and a test kit therefor Download PDFInfo
- Publication number
- GB1563299A GB1563299A GB53444/76A GB5344476A GB1563299A GB 1563299 A GB1563299 A GB 1563299A GB 53444/76 A GB53444/76 A GB 53444/76A GB 5344476 A GB5344476 A GB 5344476A GB 1563299 A GB1563299 A GB 1563299A
- Authority
- GB
- United Kingdom
- Prior art keywords
- solution
- hcg
- igg
- mixture
- anti hcg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 30
- 238000012360 testing method Methods 0.000 title claims description 29
- 230000035935 pregnancy Effects 0.000 title claims description 9
- 239000000243 solution Substances 0.000 claims description 36
- 102000004190 Enzymes Human genes 0.000 claims description 29
- 108090000790 Enzymes Proteins 0.000 claims description 29
- 239000000203 mixture Substances 0.000 claims description 26
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 210000001124 body fluid Anatomy 0.000 claims description 11
- 239000010839 body fluid Substances 0.000 claims description 11
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims description 8
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 6
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 claims description 5
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 5
- 210000002700 urine Anatomy 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 241000283707 Capra Species 0.000 claims description 4
- HRNLUBSXIHFDHP-UHFFFAOYSA-N N-(2-aminophenyl)-4-[[[4-(3-pyridinyl)-2-pyrimidinyl]amino]methyl]benzamide Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC1=NC=CC(C=2C=NC=CC=2)=N1 HRNLUBSXIHFDHP-UHFFFAOYSA-N 0.000 claims description 4
- 229920005654 Sephadex Polymers 0.000 claims description 4
- 239000012507 Sephadex™ Substances 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 4
- 241001494479 Pecora Species 0.000 claims description 3
- 102000003992 Peroxidases Human genes 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 2
- 108060003951 Immunoglobulin Proteins 0.000 claims description 2
- 239000007836 KH2PO4 Substances 0.000 claims description 2
- 239000004677 Nylon Substances 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 239000000872 buffer Substances 0.000 claims description 2
- 239000008366 buffered solution Substances 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 2
- 235000019800 disodium phosphate Nutrition 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 102000018358 immunoglobulin Human genes 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 239000008363 phosphate buffer Substances 0.000 claims description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 2
- 238000011533 pre-incubation Methods 0.000 claims description 2
- 229940088598 enzyme Drugs 0.000 description 19
- 210000002966 serum Anatomy 0.000 description 5
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000007975 buffered saline Substances 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- LOTKRQAVGJMPNV-UHFFFAOYSA-N 1-fluoro-2,4-dinitrobenzene Chemical compound [O-][N+](=O)C1=CC=C(F)C([N+]([O-])=O)=C1 LOTKRQAVGJMPNV-UHFFFAOYSA-N 0.000 description 1
- ITZMJCSORYKOSI-AJNGGQMLSA-N APGPR Enterostatin Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)CCC1 ITZMJCSORYKOSI-AJNGGQMLSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000269350 Anura Species 0.000 description 1
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 1
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010029719 Nonspecific reaction Diseases 0.000 description 1
- 240000006909 Tilia x europaea Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940093476 ethylene glycol Drugs 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 1
- 229940027941 immunoglobulin g Drugs 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- STEPQTYSZVCJPV-UHFFFAOYSA-N metazachlor Chemical compound CC1=CC=CC(C)=C1N(C(=O)CCl)CN1N=CC=C1 STEPQTYSZVCJPV-UHFFFAOYSA-N 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL48741A IL48741A (en) | 1975-12-26 | 1975-12-26 | Method for the determination of pregnancy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1563299A true GB1563299A (en) | 1980-03-26 |
Family
ID=11048617
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB53444/76A Expired GB1563299A (en) | 1975-12-26 | 1976-12-21 | Methods of pregnancy testing and a test kit therefor |
Country Status (14)
| Country | Link |
|---|---|
| JP (1) | JPS5282892A (enExample) |
| AT (1) | AT352904B (enExample) |
| AU (1) | AU2049576A (enExample) |
| BE (1) | BE849667A (enExample) |
| DE (1) | DE2657292A1 (enExample) |
| ES (1) | ES454542A1 (enExample) |
| FI (1) | FI763647A7 (enExample) |
| FR (1) | FR2336113A1 (enExample) |
| GB (1) | GB1563299A (enExample) |
| IL (1) | IL48741A (enExample) |
| IT (1) | IT1068738B (enExample) |
| NL (1) | NL7614359A (enExample) |
| SE (1) | SE7614387L (enExample) |
| ZA (1) | ZA767607B (enExample) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0062892A1 (en) * | 1981-04-13 | 1982-10-20 | American Hoechst Corporation | Single incubation immunochemical assay for creatin phosphokinase MB |
| EP0074520A1 (en) * | 1981-09-16 | 1983-03-23 | Teva Pharmaceutical Industries Limited | Method and kit for pregnancy detection |
| US4419453A (en) | 1981-09-28 | 1983-12-06 | The Dow Chemical Company | Immunological agglutination assays with dyed or colored latex and kits |
| US4543339A (en) * | 1982-03-16 | 1985-09-24 | Oneill Christopher | Early pregnancy detection by detecting enhanced blood platelet activation |
| WO1986005498A1 (en) * | 1985-03-12 | 1986-09-25 | University Of Queensland | Method for detecting early pregnancy factor (epf) in mammals, purifying epf and method for producing a monoclonal antibody |
| EP0210863A1 (en) * | 1985-07-29 | 1987-02-04 | Modern Diagnostics, Inc. | Immunoassay |
| DE3590722T1 (enExample) * | 1982-08-20 | 1987-11-19 | ||
| US7034120B2 (en) | 1999-02-02 | 2006-04-25 | Edp Biotech Corporation, Inc. | Method and apparatus for detecting conception in animals |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1337394C (en) * | 1987-11-17 | 1995-10-24 | Nelson N. H. Teng | Vaginal sample test and reagents |
-
1975
- 1975-12-26 IL IL48741A patent/IL48741A/xx unknown
-
1976
- 1976-12-13 AU AU20495/76A patent/AU2049576A/en not_active Expired
- 1976-12-15 JP JP15067576A patent/JPS5282892A/ja active Pending
- 1976-12-17 DE DE19762657292 patent/DE2657292A1/de active Pending
- 1976-12-20 FR FR7638333A patent/FR2336113A1/fr not_active Withdrawn
- 1976-12-20 FI FI763647A patent/FI763647A7/fi not_active Application Discontinuation
- 1976-12-21 BE BE173475A patent/BE849667A/xx unknown
- 1976-12-21 SE SE7614387A patent/SE7614387L/xx unknown
- 1976-12-21 IT IT70054/76A patent/IT1068738B/it active
- 1976-12-21 GB GB53444/76A patent/GB1563299A/en not_active Expired
- 1976-12-22 ZA ZA767607A patent/ZA767607B/xx unknown
- 1976-12-23 ES ES454542A patent/ES454542A1/es not_active Expired
- 1976-12-23 AT AT962176A patent/AT352904B/de not_active IP Right Cessation
- 1976-12-23 NL NL7614359A patent/NL7614359A/xx not_active Application Discontinuation
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0062892A1 (en) * | 1981-04-13 | 1982-10-20 | American Hoechst Corporation | Single incubation immunochemical assay for creatin phosphokinase MB |
| EP0074520A1 (en) * | 1981-09-16 | 1983-03-23 | Teva Pharmaceutical Industries Limited | Method and kit for pregnancy detection |
| US4419453A (en) | 1981-09-28 | 1983-12-06 | The Dow Chemical Company | Immunological agglutination assays with dyed or colored latex and kits |
| US4543339A (en) * | 1982-03-16 | 1985-09-24 | Oneill Christopher | Early pregnancy detection by detecting enhanced blood platelet activation |
| DE3590722T1 (enExample) * | 1982-08-20 | 1987-11-19 | ||
| WO1986005498A1 (en) * | 1985-03-12 | 1986-09-25 | University Of Queensland | Method for detecting early pregnancy factor (epf) in mammals, purifying epf and method for producing a monoclonal antibody |
| GB2192634B (en) * | 1985-03-12 | 1990-03-21 | Univ Queensland | Method of detecting early pregnancy factor (epf) in mammals, purifying epf and method for providing a monoclonal antibody |
| EP0210863A1 (en) * | 1985-07-29 | 1987-02-04 | Modern Diagnostics, Inc. | Immunoassay |
| US7179640B2 (en) | 1998-02-02 | 2007-02-20 | Edp Biotech Corporation | Method and apparatus for detecting conception in animals |
| US7034120B2 (en) | 1999-02-02 | 2006-04-25 | Edp Biotech Corporation, Inc. | Method and apparatus for detecting conception in animals |
Also Published As
| Publication number | Publication date |
|---|---|
| ES454542A1 (es) | 1978-04-01 |
| AU2049576A (en) | 1978-06-22 |
| BE849667A (fr) | 1977-06-21 |
| IT1068738B (it) | 1985-03-21 |
| NL7614359A (nl) | 1977-06-28 |
| FI763647A7 (enExample) | 1977-06-27 |
| IL48741A0 (en) | 1976-02-29 |
| ZA767607B (en) | 1977-11-30 |
| FR2336113A1 (fr) | 1977-07-22 |
| DE2657292A1 (de) | 1977-07-14 |
| ATA962176A (de) | 1979-03-15 |
| SE7614387L (sv) | 1977-06-27 |
| IL48741A (en) | 1979-07-25 |
| AT352904B (de) | 1979-10-10 |
| JPS5282892A (en) | 1977-07-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| CSNS | Application of which complete specification have been accepted and published, but patent is not sealed |