GB1511082A - Hbsag particle and a method of preparing such particles - Google Patents

Hbsag particle and a method of preparing such particles

Info

Publication number
GB1511082A
GB1511082A GB727976A GB727976A GB1511082A GB 1511082 A GB1511082 A GB 1511082A GB 727976 A GB727976 A GB 727976A GB 727976 A GB727976 A GB 727976A GB 1511082 A GB1511082 A GB 1511082A
Authority
GB
United Kingdom
Prior art keywords
hbsag
surfactant
particles
oxyethylene
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB727976A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GREEN CROSS CORP
Original Assignee
GREEN CROSS CORP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GREEN CROSS CORP filed Critical GREEN CROSS CORP
Priority to GB727976A priority Critical patent/GB1511082A/en
Publication of GB1511082A publication Critical patent/GB1511082A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • G01N33/5761—Hepatitis B
    • G01N33/5764—Hepatitis B surface antigen

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Communicable Diseases (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

1511082 HBsAg GREEN CROSS CORP 24 Feb 1976 7279/76 Heading C3H A hollow spherical particle of HBsAg is characterized in having a diameter of 18-22 n.m. inclusive and being composed of single polypeptide subunits each having a molecular weight of about 55,000 dentons. Hollow particles of HBsAg having these characteristics may be prepared in a method which includes the step of heating HBsAg of a medium containing it with a surfactant capable of delipidation in isotonic sodium chloride at a pH of 5-9 inclusive to obtain the said HBsAg particles in an aqueous medium. The surfactant may be a polyoxyethylene alkylphenol containing 7-10 molecules of oxyethylene, e.g. a polyoxyethylene octylphenol containing a molecules of oxyethylene; a polyoxyethylene sorbitan monoalkyl ester containing 20 molecules of oxyethylene, e.g. a polyoxyethylene sorbitan monooleate; an alkali metal salt of a bile acid, e.g. sodium deoxycholate, glycocholate, taurocholate or dehydrocholate; or an alkali metal salt of lauroylsarcosinic acid, e.g. sodium lauroylsarcosinate. The concentration of the surfactant may be 0À05-5% by weight, and the heat treatment may be effected for 5-120 minutes at 40-80‹ C. The HBsAg material used in the method may be obtained from serum or plasma by known procedures and it is preferred to use a fraction containing alpha- and betaglobulins. It may be purified prior to being subjected to the heating step by conventional techniques, e.g. by subjecting it to gel filtration. The product of the method may be isolated from other proteins by known methods, e.g. ion exchange chromatography, fractional precipitation or gel filtration, e.g. with dextron, polyacrylamide or organose. The surfactant may be removed from the aqueous medium containing the particles by dialysis or gel filtration of the aqueous emulsion.
GB727976A 1976-02-24 1976-02-24 Hbsag particle and a method of preparing such particles Expired GB1511082A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB727976A GB1511082A (en) 1976-02-24 1976-02-24 Hbsag particle and a method of preparing such particles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB727976A GB1511082A (en) 1976-02-24 1976-02-24 Hbsag particle and a method of preparing such particles

Publications (1)

Publication Number Publication Date
GB1511082A true GB1511082A (en) 1978-05-17

Family

ID=9830057

Family Applications (1)

Application Number Title Priority Date Filing Date
GB727976A Expired GB1511082A (en) 1976-02-24 1976-02-24 Hbsag particle and a method of preparing such particles

Country Status (1)

Country Link
GB (1) GB1511082A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3417406A1 (en) * 1984-05-10 1986-01-02 Gödecke AG, 1000 Berlin Antiserum against hepatitis B surface antigen from sheep, horses or goats and process for its preparation
EP1985711A1 (en) * 2006-02-13 2008-10-29 Japan Science and Technology Agency Method for efficient purification of bionanocapsule

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3417406A1 (en) * 1984-05-10 1986-01-02 Gödecke AG, 1000 Berlin Antiserum against hepatitis B surface antigen from sheep, horses or goats and process for its preparation
EP1985711A1 (en) * 2006-02-13 2008-10-29 Japan Science and Technology Agency Method for efficient purification of bionanocapsule
EP1985711A4 (en) * 2006-02-13 2009-09-16 Japan Science & Tech Agency Method for efficient purification of bionanocapsule

Similar Documents

Publication Publication Date Title
Lin et al. Cyclic 3′: 5′-nucleotide phosphodiesterase: purification, characterization, and active form of the protein activator from bovine brain
Cutting et al. Staining of phospho-proteins on acrylamide gel electropherograms
Agrawal et al. Protein-carbohydrate interaction: VII. Physical and chemical studies on concanavalin A, the hemagglutinin of the jack bean
US4217339A (en) Glycoprotein and process for isolating it
Palfree et al. Yeast killer toxin: purification and characterisation of the protein toxin from Saccharomyces cerevisiae
Shaw et al. Description of two procedures which avoid the use of extreme pH conditions for the resolution of components isolated from chromatins prepared from pig cerebellar and pituitary nuclei
US4287184A (en) Process for healing wounds
Scanu et al. Separation and characterization of the protein moiety of human α1-lipoprotein
Margolis et al. Studies on Human Serum β1-Lipoprotein: I. AMINO ACID COMPOSITION
Jockusch Two mutants of tobacco mosaic virus temperature-sensitive in two different functions
Schnebli et al. Membrane adenosine triphosphatase from Streptococcus faecalis: molecular weight, subunit structure, and amino acid composition
Burke The purification of interferon
McIntosh et al. Multiple forms of acetylcholinesterase from pig brain
Hoch et al. Albumin from heated human plasma. I. Preparation and electrophoretic properties
US4191533A (en) Pregnancy-specific β1 -glycoprotein and process for isolating it
US4407744A (en) Process for obtaining nerve growth factor
US4185095A (en) Nerve growth factor
NZ187548A (en) Glycoprotein and antiserum
Tuszynski et al. Recovery of concentrated protein samples from sodium dodecyl sulfate-polyacrylamide gels
Freyssinet et al. The chloroplast and cytoplasmic ribosomes of Euglena: ii. characterization of ribosomal proteins
GB1511082A (en) Hbsag particle and a method of preparing such particles
US3975344A (en) Interferon purification
Stubbs et al. Amino acid incorporation in an Escherichia coli cell-free system directed by bromegrass mosaic virus ribonucleic acid
Matthews et al. Shell formation by capsid protein of f2 bacteriophage
Schick Influence of a cationic detergent on electrophoresis in polyacrylamide gel

Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19930224