GB1488988A - Stabilising agent for enzymes - Google Patents

Stabilising agent for enzymes

Info

Publication number
GB1488988A
GB1488988A GB4836375A GB4836375A GB1488988A GB 1488988 A GB1488988 A GB 1488988A GB 4836375 A GB4836375 A GB 4836375A GB 4836375 A GB4836375 A GB 4836375A GB 1488988 A GB1488988 A GB 1488988A
Authority
GB
United Kingdom
Prior art keywords
enzymes
precipitate
dehydrogenase
stabilizing agent
yeast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4836375A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to GB4836375A priority Critical patent/GB1488988A/en
Publication of GB1488988A publication Critical patent/GB1488988A/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/45—Transferases (2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/44—Oxidoreductases (1)
    • A61K38/443—Oxidoreductases (1) acting on CH-OH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03004—Glucose oxidase (1.1.3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/0103—Glycerol kinase (2.7.1.30)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Inorganic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

1488988 Stabilizing agent for enzymes BOEHRINGER MANNHEIM GmbH 25 Nov 1975 48363/75 Heading C3H A stabilizing agent for labile enzymes comprises a protein fraction which is the precipitate obtained by the action of trichloroacetic acid (5-20 wt. per cent/vol.) on the clear extract obtained by the extraction of digested yeast or yeast waste with water at a temperature of from 80‹ to 100‹ C. and separation of the precipitate. The precipitate may be further purified by dissolution in water at substantially neutral pH, subjected to ethanol fraction precipitation wherein the precipitate obtained with from 45 to 90% by volume of ethanol, based on the volume of solution is recovered, taken up in a buffer of substantially neutral pH, dialysed against said buffer and, optionally, subjected to chromatographic purification. The stabilizing agent is preferably in the form of a solution of pH above 5 with a protein content of from 5 to 200 mg./ml. In the examples, the enzymes stabilized are glycerokinase (from Candida mycoderma), glucose 6-phosphate dehydrogenase, glycerokinase/ Aspergillus niger composition, maleate dehydrogenase (from cardiac muscle), glucose dehydrogenase (from Bacillus subtilis), aldehyde dehydrogenase (from baker's yeast) and glucose oxidase (from mould fungus).
GB4836375A 1975-11-25 1975-11-25 Stabilising agent for enzymes Expired GB1488988A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB4836375A GB1488988A (en) 1975-11-25 1975-11-25 Stabilising agent for enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB4836375A GB1488988A (en) 1975-11-25 1975-11-25 Stabilising agent for enzymes

Publications (1)

Publication Number Publication Date
GB1488988A true GB1488988A (en) 1977-10-19

Family

ID=10448350

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4836375A Expired GB1488988A (en) 1975-11-25 1975-11-25 Stabilising agent for enzymes

Country Status (1)

Country Link
GB (1) GB1488988A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450898A2 (en) * 1990-04-03 1991-10-09 Microgenics Corporation Stabilization of polypeptide fragments against proteolytic degradation
EP2493915A1 (en) * 2009-10-26 2012-09-05 EyeSense AG Stabilization of bio-sensors for in vivo applications
CN110029093A (en) * 2016-12-14 2019-07-19 中国农业科学院农业资源与农业区划研究所 Recombinant glucose dehydrogenase and preparation method thereof and code used gene

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450898A2 (en) * 1990-04-03 1991-10-09 Microgenics Corporation Stabilization of polypeptide fragments against proteolytic degradation
EP0450898A3 (en) * 1990-04-03 1992-12-09 Microgenics Corporation Stabilization of polypeptide fragments against proteolytic degradation
US5212081A (en) * 1990-04-03 1993-05-18 Microgenics Corporation Stabilization of polypeptide fragments against proteolytic degradation
EP2493915A1 (en) * 2009-10-26 2012-09-05 EyeSense AG Stabilization of bio-sensors for in vivo applications
US8945942B2 (en) 2009-10-26 2015-02-03 Eyesense Ag Stabilization of bio-sensors for in vivo applications
EP2493915B1 (en) * 2009-10-26 2017-08-16 EyeSense AG Stabilisation of biosensors for in vivo applications
CN110029093A (en) * 2016-12-14 2019-07-19 中国农业科学院农业资源与农业区划研究所 Recombinant glucose dehydrogenase and preparation method thereof and code used gene
CN110029093B (en) * 2016-12-14 2020-07-28 中国农业科学院农业资源与农业区划研究所 Recombinant glucose dehydrogenase and preparation method and encoding gene thereof

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee