GB1478575A - Apparatus for enumerating microorganisms - Google Patents

Apparatus for enumerating microorganisms

Info

Publication number
GB1478575A
GB1478575A GB3117675A GB3117675A GB1478575A GB 1478575 A GB1478575 A GB 1478575A GB 3117675 A GB3117675 A GB 3117675A GB 3117675 A GB3117675 A GB 3117675A GB 1478575 A GB1478575 A GB 1478575A
Authority
GB
United Kingdom
Prior art keywords
membrane
cells
enumerating
colonies
barrier material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3117675A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canadian Patents and Development Ltd
Original Assignee
Canadian Patents and Development Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Canadian Patents and Development Ltd filed Critical Canadian Patents and Development Ltd
Publication of GB1478575A publication Critical patent/GB1478575A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1478575 Apparatus for enumerating microorganisms CANADIAN PATENTS & DEVELOPMENT Ltd 25 July 1975 [14 Aug 1974] 31176/75 Heading C6F An apparatus for enumerating micro-organisms consists essentially of a membrane filter element 1, Figs.1 and 2, for retaining on a surface thereof the micro-organisms present in a fluid sample filtered thereby, which surface carries an imprinted pattern 2 of a barrier material defining a plurality of ordered, microbial colony-isolating cells 3. The cells are of an area smaller than the area normally expected for the microbial colonies 4 so that these tend to grow perpendicular to the surface of the membrane, increasing in thickness and thus enhancing the optical contrast between colony and filter. Overlapping and confluent growth of the colonies is prevented. In general the area of each cell is not greater than 25 mm<SP>2</SP> and the width of the barrier material is greater than 0À025 mm. Barrier materials: paraffin wax, petrolatum, silicone wax or oil, epoxy resin, polytetrafluoroethylene or polystyrene solutions, rubber, acrylic resin and beeswax. The gridded membrane 21, Figs.5 and 6, may be mounted in a stackable container 25 upon an absorbent pad 29 for retaining nutrient solution. In other embodiments the cells are arranged on the membrane in the form of a spiral and the barrier material defines circular cells, Figs.3 and 4 respectively (not shown). An Example is directed to enumerating colonies of Serratia marcescens using cellulose ester membranes imprinted with a square grid pattern of paraffin wax with lines 0À125 mm wide and 0À5 mm apart.
GB3117675A 1974-08-14 1975-07-25 Apparatus for enumerating microorganisms Expired GB1478575A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US49728574A 1974-08-14 1974-08-14

Publications (1)

Publication Number Publication Date
GB1478575A true GB1478575A (en) 1977-07-06

Family

ID=23976223

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3117675A Expired GB1478575A (en) 1974-08-14 1975-07-25 Apparatus for enumerating microorganisms

Country Status (5)

Country Link
JP (1) JPS5144973A (en)
CA (1) CA1032453A (en)
DE (1) DE2536163C3 (en)
FR (1) FR2281983A1 (en)
GB (1) GB1478575A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2187474A (en) * 1986-02-21 1987-09-09 Valio Meijerien Method for determining microbe concentration by means of the plating method and dishes used therein

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52146370U (en) * 1976-04-27 1977-11-07
DE19818184A1 (en) * 1998-04-23 1999-10-28 Queisser Pharma Gmbh & Co Determining the presence of microorganisms in compressed air, useful for pharmaceutical industry

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2187474A (en) * 1986-02-21 1987-09-09 Valio Meijerien Method for determining microbe concentration by means of the plating method and dishes used therein
AU591236B2 (en) * 1986-02-21 1989-11-30 Valio Meijerien Keskusosuusliike A method for the determination of microbe concentrations by means of a plating method and dishes used therein
GB2187474B (en) * 1986-02-21 1990-08-15 Valio Meijerien A method for the determination of microbe concentrations by means of a plating method and dishes used therein
US5134064A (en) * 1986-02-21 1992-07-28 Valio Meijerien Keskusosuusliike Method for the determination of microbe concentrations by means of a plating method

Also Published As

Publication number Publication date
DE2536163C3 (en) 1979-10-31
FR2281983A1 (en) 1976-03-12
DE2536163B2 (en) 1979-03-08
DE2536163A1 (en) 1976-02-26
JPS5539B2 (en) 1980-01-05
FR2281983B1 (en) 1977-12-16
CA1032453A (en) 1978-06-06
JPS5144973A (en) 1976-04-16

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee