GB1475318A - Method for differentially counting leukocytes and other cells - Google Patents

Method for differentially counting leukocytes and other cells

Info

Publication number
GB1475318A
GB1475318A GB4106676A GB4106676A GB1475318A GB 1475318 A GB1475318 A GB 1475318A GB 4106676 A GB4106676 A GB 4106676A GB 4106676 A GB4106676 A GB 4106676A GB 1475318 A GB1475318 A GB 1475318A
Authority
GB
United Kingdom
Prior art keywords
radiation
leukocytes
leukocyte types
leukocyte
blood
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4106676A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Block Engineering Inc
Original Assignee
Block Engineering Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US365460A external-priority patent/US3916205A/en
Priority claimed from US05/438,162 external-priority patent/US4146604A/en
Application filed by Block Engineering Inc filed Critical Block Engineering Inc
Publication of GB1475318A publication Critical patent/GB1475318A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Ecology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1475318 Testing blood BLOCK ENG Inc 19 April 1974 [31 May 1973 30 Jan 1974] 41066/76 Divided out of 1475317 Heading G1A A system for differentially counting leukocyte types randomly distributed in a blood medium comprises (a) means (not shown), for staining the leukocyte types with a dye composition comprising a plurality of dyes, at least one of the dyes imparting a first fluorescence to the nuclei of all leukocytes and another dye imparting a second fluorescence to some leukocyte types, (b) flow means 52 through which the stained leukocytes in the blood medium under test are to pass in single file, (c) source means 54 &c. for illuminating the stained leukocytes passing through the flow means, with radiation in the characteristic absorption wavelength region of each of the dyes, and (d) detection means 81, 74, 80 optically coupled to the flow means for detecting the leukocytes passing through the flow means in single file, the detector means distinguishing and counting the leukocyte types according to the relationships between the intensities of the radiation emitted by each leukocyte type in characteristic emission wavelength regions. In the embodiment shown, source 54 is a mercury arc lamp, and the radiation passes through collimating lens 56, reflecting filter 58, for example a dichroic filter, condensing lens 64 or 72 to focus on capillary tube flow path 52 at 62 or 70. Photo-multipliers 74, 80 and photo-diode 81 detect emitted radiation in different respective wavelength ranges and the signals from the photo-electric devices are passed to processor 86 where in the embodiment described there is calculated the ratio of the measured intensities of radiation of green and of red wavelengths to distinguish the different leukocyte types for counting. Display device 88 shows the results. In a related embodiment, Fig. 1 (not shown), the leukocytes are stained in the form of an alcoholfixed blood smear on a slide and the incident and transmitted radiation paths each include a filter wheel (18, 28), which carries two filters capable of transmitting light in different wavelength bands. The detection system comprises an image scanning device. The invention may be used to distinguish and count erythrocytes, reticulocytes and leukocyte types in a blood sample.
GB4106676A 1973-05-31 1974-04-19 Method for differentially counting leukocytes and other cells Expired GB1475318A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US365460A US3916205A (en) 1973-05-31 1973-05-31 Differential counting of leukocytes and other cells
US05/438,162 US4146604A (en) 1973-05-31 1974-01-30 Differential counting of leukocytes and other cells
GB1723474A GB1475317A (en) 1973-05-31 1974-04-19 Method and composition for staining biological material

Publications (1)

Publication Number Publication Date
GB1475318A true GB1475318A (en) 1977-06-01

Family

ID=27257478

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4106676A Expired GB1475318A (en) 1973-05-31 1974-04-19 Method for differentially counting leukocytes and other cells

Country Status (1)

Country Link
GB (1) GB1475318A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4225783A (en) * 1978-07-24 1980-09-30 Abbott Laboratories Determination of microbial cells in aqueous samples
EP0266881A2 (en) * 1986-09-30 1988-05-11 Astromed Limited Method and apparatus for multiple optical assaying
EP1065496A2 (en) * 1999-06-29 2001-01-03 Tripath Imaging, Inc. Method and apparatus for deriving separate images from multiple chromogens in a biological specimen

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4225783A (en) * 1978-07-24 1980-09-30 Abbott Laboratories Determination of microbial cells in aqueous samples
EP0266881A2 (en) * 1986-09-30 1988-05-11 Astromed Limited Method and apparatus for multiple optical assaying
EP0266881A3 (en) * 1986-09-30 1990-04-04 Astromed Limited Method and apparatus for multiple optical assaying
EP1065496A2 (en) * 1999-06-29 2001-01-03 Tripath Imaging, Inc. Method and apparatus for deriving separate images from multiple chromogens in a biological specimen
EP1065496A3 (en) * 1999-06-29 2003-11-05 Tripath Imaging, Inc. Method and apparatus for deriving separate images from multiple chromogens in a biological specimen

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee