GB1452231A - Method and apparatus for detecting and classifying nucleic acid particles - Google Patents

Method and apparatus for detecting and classifying nucleic acid particles

Info

Publication number
GB1452231A
GB1452231A GB2279074A GB2279074A GB1452231A GB 1452231 A GB1452231 A GB 1452231A GB 2279074 A GB2279074 A GB 2279074A GB 2279074 A GB2279074 A GB 2279074A GB 1452231 A GB1452231 A GB 1452231A
Authority
GB
United Kingdom
Prior art keywords
radiation
fluorescent
nucleic acid
filter
optics
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2279074A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Block Engineering Inc
Original Assignee
Block Engineering Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Block Engineering Inc filed Critical Block Engineering Inc
Publication of GB1452231A publication Critical patent/GB1452231A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1452231 Classifying nucleic acid containing particles BLOCK ENG Inc 22 May 1974 [2 July 1973] 22790/74 Heading G1A Particles containing nucleic acid and treated with a fluorescent dye are classified in a fluid in apparatus which illuminates the specimen with excitation radiation and views the fluorescence and which spatially filters at least one of the illuminating and fluorescent radiation. Classification by size is obtained by analyzing the fluorescent radiation for modulation at different low frequencies due to Brownian movement. By using suitable dyes and detectors responsive to a single frequency RNA and DNA containing particles e.g. viruses can be distinguished. The percentage of DNA and the particle shapes can be derived from polarization measurements on scattered radiation. As shown, light from source 12 which may be a laser passes through wavelength filter 14 and spatial filter 18 which may comprise a grating, grid or annuli. The filter is focused on to sample 20 by optics 16. The fluorescent and transmitted radiation passes through optics 22 and is separated by splitter 24 on to detectors 26, 28, 30 which may be frequency sensitive or have associated filters 32, 34. Detector 30 has a mask 48 to block out direct light and the mask may be associated with a polarization detector. The detected signals are amplified, analyzed by frequency analyzers 42- 46 and recorded on strip charts or fed to a computer.
GB2279074A 1973-07-02 1974-05-22 Method and apparatus for detecting and classifying nucleic acid particles Expired GB1452231A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US375807A US3872312A (en) 1973-07-02 1973-07-02 Method and apparatus for detecting and classifying nucleic acid particles

Publications (1)

Publication Number Publication Date
GB1452231A true GB1452231A (en) 1976-10-13

Family

ID=23482435

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2279074A Expired GB1452231A (en) 1973-07-02 1974-05-22 Method and apparatus for detecting and classifying nucleic acid particles

Country Status (9)

Country Link
US (1) US3872312A (en)
JP (1) JPS5050093A (en)
AU (1) AU476320B2 (en)
CA (1) CA1002343A (en)
CH (1) CH582882A5 (en)
DE (1) DE2431203A1 (en)
FR (1) FR2236180B1 (en)
GB (1) GB1452231A (en)
NL (1) NL7407776A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2196734A (en) * 1986-09-30 1988-05-05 Astromed Ltd Multiple assaying technique

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4571388A (en) * 1983-01-24 1986-02-18 Becton Dickinson And Company Detection of reticulocytes
NZ207393A (en) * 1983-08-05 1987-03-31 Neal Lloyd First Staining dna in living cells
SE455646B (en) * 1984-10-22 1988-07-25 Radians Innova Ab FLUORESCENT DEVICE
US4707451A (en) * 1985-09-18 1987-11-17 Becton, Dickinson And Company Detection of reticulocytes
US4883867A (en) * 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
EP0226272B1 (en) * 1985-11-01 1991-03-06 Becton, Dickinson and Company Detection of reticulocytes
US4957870A (en) * 1985-11-01 1990-09-18 Becton, Dickinson And Company Detection of Reticulocytes, RNA and DNA
DE68924749T2 (en) * 1988-09-15 1996-07-04 Univ Arkansas Identification of particles by modulated dynamic light scattering.
US5350697A (en) * 1990-08-28 1994-09-27 Akzo N.V. Scattered light detection apparatus
AU5884394A (en) * 1993-01-18 1994-08-15 Evotec Biosystems Gmbh Method and device for assessing the suitability of biopolymers
US7241569B2 (en) * 1993-01-18 2007-07-10 Olympus Corporation Method and a device for the evaluation of biopolymer fitness
IL108497A0 (en) * 1993-02-01 1994-05-30 Seq Ltd Methods and apparatus for dna sequencing
US5382512A (en) * 1993-08-23 1995-01-17 Chiron Corporation Assay device with captured particle reagent
IL113805A0 (en) * 1994-05-23 1995-08-31 Coulter Corp Detection of reticulocytes
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US9040305B2 (en) 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US7914734B2 (en) 2007-12-19 2011-03-29 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
US9714897B2 (en) 2015-02-09 2017-07-25 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same
CN106769709B (en) * 2016-12-08 2019-03-26 清华大学深圳研究生院 A kind of particulate matter method for measuring shape of palaemon and device based on polarization scattering feature

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3497690A (en) * 1967-09-21 1970-02-24 Bausch & Lomb Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof
US3657537A (en) * 1970-04-03 1972-04-18 Bausch & Lomb Computerized slit-scan cyto-fluorometer for automated cell recognition
US3864571A (en) * 1971-02-10 1975-02-04 Wheeler International Inc Method and Apparatus for Automatically, Identifying and Counting Various Cells in Body Fluids

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2196734A (en) * 1986-09-30 1988-05-05 Astromed Ltd Multiple assaying technique

Also Published As

Publication number Publication date
AU476320B2 (en) 1976-09-16
DE2431203A1 (en) 1975-01-23
AU6954774A (en) 1975-12-04
NL7407776A (en) 1975-01-06
FR2236180A1 (en) 1975-01-31
US3872312A (en) 1975-03-18
CH582882A5 (en) 1976-12-15
JPS5050093A (en) 1975-05-06
FR2236180B1 (en) 1979-03-16
CA1002343A (en) 1976-12-28

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee