GB1441543A - Immobilized enzyme compositions - Google Patents

Immobilized enzyme compositions

Info

Publication number
GB1441543A
GB1441543A GB1792474A GB1792474A GB1441543A GB 1441543 A GB1441543 A GB 1441543A GB 1792474 A GB1792474 A GB 1792474A GB 1792474 A GB1792474 A GB 1792474A GB 1441543 A GB1441543 A GB 1441543A
Authority
GB
United Kingdom
Prior art keywords
dvbz
methyl
enzymes
enzyme
vinylpyridene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1792474A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Denka Co Ltd
Original Assignee
Denki Kagaku Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP48060678A external-priority patent/JPS5120576B2/ja
Priority claimed from JP6067973A external-priority patent/JPS5317674B2/ja
Application filed by Denki Kagaku Kogyo KK filed Critical Denki Kagaku Kogyo KK
Publication of GB1441543A publication Critical patent/GB1441543A/en
Expired legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/003—Catalysts comprising hydrides, coordination complexes or organic compounds containing enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/02—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides
    • B01J31/06—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides containing polymers
    • B01J31/08—Ion-exchange resins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/26—Catalysts comprising hydrides, coordination complexes or organic compounds containing in addition, inorganic metal compounds not provided for in groups B01J31/02 - B01J31/24
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/26—Catalysts comprising hydrides, coordination complexes or organic compounds containing in addition, inorganic metal compounds not provided for in groups B01J31/02 - B01J31/24
    • B01J31/28—Catalysts comprising hydrides, coordination complexes or organic compounds containing in addition, inorganic metal compounds not provided for in groups B01J31/02 - B01J31/24 of the platinum group metals, iron group metals or copper
    • B01J31/30—Halides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/40—Regeneration or reactivation
    • B01J31/4007—Regeneration or reactivation of catalysts containing polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/02—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides
    • B01J31/0234—Nitrogen-, phosphorus-, arsenic- or antimony-containing compounds
    • B01J31/0235—Nitrogen containing compounds
    • B01J31/0239—Quaternary ammonium compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/584—Recycling of catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Materials Engineering (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

1441543 Insolubilized enzymes DENKI KAGAKU KOGYO KK 24 April 1974 [30 May 1973 (2)] 17924/74 Heading C3H [Also in Division C2] A water-insoluble enzyme composition comprises an enzyme or cells of micro-organisms containing enzymes bound to a hydrophilic, water-insoluble anion-exchange resin, said resin being a polymer which contains a quaternized pyridine ring in the polymer unit and preferably having an anion-exchange capacity of 2-5 meq./g. The pyridene unit is derived from 2- vinylpyridine (2-VP), 4-vinylpyridene (4-VP), 2 - methyl - 5 - vinylpyridene, 5 - methyl - 2- vinylpyridene, 3 - allylpyridene or 1 - vinylquinoline which is copolymerized with one or more of monomers selected from styrene (St), alpha - methylstyrene, halogenated styrenes, ethylene, propylene, acryl acid, methacrylic acid, acrolein, methylmethacrylate (MMA), vinylacetate, acrylonitrile, vinylchloride, acrylamide, butadiene, isoprene, chloroprene, divinylbenzene (DVBz), ethylene glycol dimethacrylate (EDMA), butane diolacrylate, polyethylene glycol dimethacrylate, diallylphthalate and methylenebis - (acrylamide) and, optionally, with polymers selected from polybutadiene, polyisoprene, polychloroprene, styrene/butadiene copolymer, polypropylene, chlorinated polyethylene and polyvinylalcohol. The copolymers are prepared by random, block or graft. polymerization, depending on the physical form required for the final product. When random polymerization is used, cross-linking monomers must be present to prevent the copolymer dissolving on quaternization (VP, 20-99 mole per cent; divinyl compounds, 0À5-30 mole per cent) whereas this is not necessary for graft and block copolymers (VP, 25-75 mole per cent). The base polymer is quaternized with methyl chloride, methyl bromide, methyl iodide and propyl bromide preferably, but other agents may be used (including sulphur di- and trioxides, thionyl chloride, dimethyl sulphate; acid chlorides and anhydrides; metal halide compounds and acidic compounds having high molecular weights). Enzymes specified for insolubilization are protease, aminoacylase, adenosine deaminase, AMP deaminase, amidase, α-amylase, #-amylase, glucoamylase, succinyloglucoamylase, lactic dehydrogenase, trypsin, papain, ribonuclease, dextranase, glucose oxidase, penicillin acylase, chymotrypsin, ficin, pepsin, carboxypectidase, streptokinase, urease, invertase, maltase, lactase, lipase, cellulase, catalase, melibiase, tyrosinase, aspartase, glucose isomerase, phenol oxidase, racemase and cells of microorganisms which retain the activities of said enzymes. Quaternized base polymers prepared are: P1-P3, 2-VP/St, P4-P5, 2-VP/St/DVBz, P6, 4-VP/MMA/DVBz, P7, 2-VP/EDMA/St, P8, 4-VP/St/DVBz, P9, 2-VP/MMA/DVBz, (P1-P3 are block copolymers and P4-P9 random copolymers). Insolubilized enzyme compositions exemplified are: Ex. 1-5, aminoacylase+P1, P2, P4- P6, Ex. 6-11, glucoamylase+P1, P2, P5-P8, Ex. 12-15, glucose isomerase+P4, P6, P8, P9, Ex. 16, glucose oxidase+P5, Ex. 17-18, alkaline protease+P4, P6, Ex. 19, invertase+ P5, Ex. 20-23, succinyl glucoamylase+P5- P8. The compositions have activities analogous to the free enzyme and are used in the conversions of various substrates (see Division C2).
GB1792474A 1973-05-30 1974-04-24 Immobilized enzyme compositions Expired GB1441543A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP48060678A JPS5120576B2 (en) 1973-05-30 1973-05-30
JP6067973A JPS5317674B2 (en) 1973-05-30 1973-05-30

Publications (1)

Publication Number Publication Date
GB1441543A true GB1441543A (en) 1976-07-07

Family

ID=26401735

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1792474A Expired GB1441543A (en) 1973-05-30 1974-04-24 Immobilized enzyme compositions

Country Status (5)

Country Link
DE (1) DE2413694B2 (en)
FR (1) FR2231683B1 (en)
GB (1) GB1441543A (en)
IT (1) IT1017010B (en)
NL (1) NL172762C (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013108001A1 (en) * 2012-01-19 2013-07-25 British American Tobacco (Investments) Limited Selective separation of nitroso-containing compounds
US9265283B2 (en) 2006-12-07 2016-02-23 British American Tobacco (Investments) Limited Polymers selective for tobacco specific nitrosamines and methods of using the same

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2714629C2 (en) * 1976-03-31 1983-12-15 Denki Kagaku Kogyo K.K., Tokyo Process for the preparation of immobilized enzyme mixtures
FR2440402B1 (en) * 1978-10-30 1981-08-14 Inst Nat Rech Chimique METHOD FOR IMMOBILIZING MICRO-ORGANISMS AND APPLICATIONS THEREOF
JPS5736986A (en) * 1980-08-13 1982-02-27 Tanabe Seiyaku Co Ltd Immobilized aminoacylase agent and its preparation
JP2814266B2 (en) * 1989-07-12 1998-10-22 日本バイリーン株式会社 Microbial adsorbent and method for producing the same
JP2832904B2 (en) * 1990-07-07 1998-12-09 三菱レイヨン株式会社 Biodegradable polymer

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9243096B2 (en) 2006-12-07 2016-01-26 British American Tobacco (Investments) Limited Polymers selective for nitro-containing compounds and methods of using the same
US9265283B2 (en) 2006-12-07 2016-02-23 British American Tobacco (Investments) Limited Polymers selective for tobacco specific nitrosamines and methods of using the same
WO2013108001A1 (en) * 2012-01-19 2013-07-25 British American Tobacco (Investments) Limited Selective separation of nitroso-containing compounds
JP2015512763A (en) * 2012-01-19 2015-04-30 ブリティッシュ アメリカン タバコ （インヴェストメンツ） リミテッドＢｒｉｔｉｓｈ Ａｍｅｒｉｃａｎｔｏｂａｃｃｏ （Ｉｎｖｅｓｔｍｅｎｔｓ） Ｌｉｍｉｔｅｄ Selective separation of compounds containing nitroso groups
CN105410986A (en) * 2012-01-19 2016-03-23 英美烟草(投资)有限公司 Selective separation of nitroso-containing compounds
AU2012366784B2 (en) * 2012-01-19 2016-10-27 British American Tobacco (Investments) Limited Selective separation of nitroso-containing compounds
RU2622410C2 (en) * 2012-01-19 2017-06-15 Бритиш Америкэн Тобэкко (Инвестментс) Лимитед Selective separation nitroso-containing compounds

Also Published As

Publication number Publication date
FR2231683A1 (en) 1974-12-27
IT1017010B (en) 1977-07-20
NL7405654A (en) 1974-12-03
NL172762B (en) 1983-05-16
DE2413694C3 (en) 1979-11-22
DE2413694B2 (en) 1979-03-22
NL172762C (en) 1983-10-17
DE2413694A1 (en) 1974-12-05
FR2231683B1 (en) 1978-07-28

Similar Documents

Publication Publication Date Title
US4478976A (en) Water-insoluble protein material, its preparation and its use
Galaev et al. Thermoreactive water-soluble polymers, nonionic surfactants, and hydrogels as reagents in biotechnology
US3915797A (en) Immobilized enzymes
O'driscoll [12] Techniques of enzyme entrapment in gels
US3625827A (en) Water-soluble polymer-enzyme products
GB1441543A (en) Immobilized enzyme compositions
IE33536B1 (en) Fibres containing enzymes,process for their preparation and their use in enzymatic reactions
US4272617A (en) Immobilization of enzymes or bacteria cells
Kobayashi Mechanisms for gene conversion and homologous recombination: the double-strand break repair model and the successive half crossing-over model
GB1294591A (en) New copolymers suitable as carrier-binders for proteins and proteins bound to said copolymer carrier-binders
PL90704B1 (en)
US10808238B2 (en) Stabilization of biomolecules by attachment of responsive polymers and sensors thereof
Kumar et al. Protease production by Bacillus subtilis immobilized on different matrices
US3925157A (en) Immobilized enzymes
Kumar et al. A comparative study of immobilized lipase produced from Penicillium chrysogenum SNP5 on two different anionic carriers for its pH and thermostability
Cripps et al. The accumulation of extracellular macromolecules by Staphylococcus aureus grown in the presence of sodium chloride and glucose
Hamerska-Dudra et al. Immobilization of glucoamylase and trypsin on crosslinked thermosensitive carriers
Novick et al. Investigating the effects of polymer chemistry on activity of biocatalytic plastic materials
WO1995006132A3 (en) Identifying biologically active agents through culture color change
US4525457A (en) Method of immobilizing enzymatically active materials
WO2003087342B1 (en) Reducing non-specific binding in immunoassays performed on polymeric solid phases
Beddows et al. The immobilization of enzymes and cells of Bacillus stearothermophilus onto poly (maleic anhydride/styrene)–Co‐polyethylene and poly (maleic anhydride/vinyl acetate)–Co‐polyethylene
Moustafa et al. Preparation of porous polymeric structures for enzyme immobilization
Hamerska-Dudra et al. Novel method of enzymes stabilization on crosslinked thermosensitive carriers
US3753861A (en) Binding enzymes to carbonyl polymers

Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee