GB1389553A - Apparatus for investigating microscopic particles - Google Patents
Apparatus for investigating microscopic particlesInfo
- Publication number
- GB1389553A GB1389553A GB2406272A GB2406272A GB1389553A GB 1389553 A GB1389553 A GB 1389553A GB 2406272 A GB2406272 A GB 2406272A GB 2406272 A GB2406272 A GB 2406272A GB 1389553 A GB1389553 A GB 1389553A
- Authority
- GB
- United Kingdom
- Prior art keywords
- cell
- cells
- photo
- microscope
- plane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000002245 particle Substances 0.000 title abstract 4
- 210000004027 cell Anatomy 0.000 abstract 19
- 230000003287 optical effect Effects 0.000 abstract 8
- 238000004458 analytical method Methods 0.000 abstract 3
- 210000000601 blood cell Anatomy 0.000 abstract 2
- 210000000805 cytoplasm Anatomy 0.000 abstract 2
- 238000010191 image analysis Methods 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 238000005259 measurement Methods 0.000 abstract 2
- 239000012528 membrane Substances 0.000 abstract 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 abstract 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000003990 capacitor Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000005684 electric field Effects 0.000 abstract 1
- 238000011156 evaluation Methods 0.000 abstract 1
- 239000000835 fiber Substances 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 238000005286 illumination Methods 0.000 abstract 1
- 230000000977 initiatory effect Effects 0.000 abstract 1
- 238000011835 investigation Methods 0.000 abstract 1
- 230000002093 peripheral effect Effects 0.000 abstract 1
- 239000004033 plastic Substances 0.000 abstract 1
- 229920003023 plastic Polymers 0.000 abstract 1
- 229910052697 platinum Inorganic materials 0.000 abstract 1
- 239000011148 porous material Substances 0.000 abstract 1
- 238000004513 sizing Methods 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1029—Particle size
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Signal Processing (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1389553 Investigating microscopic particles G LEGORRETA 23 May 1972 24062/72 Headings G1A and G1N [Also in Division G2] A microscope has photo-electric image analyzing means viewing its image plane arranged to investigate particles such as blood cells as they pass sequentially along a path including the image plane. In the arrangement of Fig.1, blood cells, suspended in a liquid, are introduced into the microscope through central conduit 22. Continuous staining of the cells is accomplished by passage of dye through outer conduits 23, the walls (24) Fig.2 (not shown) separating the conduits being of porous plastics membrane, which also serves to separate off some smaller cells in accordance with the pore size of the membrane. Cells pass one by one through the focal plane 18 of the microscope, and are illuminated via annular mirror 38, and objective at 16. The light source may be an incandescent lamp, a high pressure spark lamp, an arc lamp or a laser. Light reflected or scattered by the cell at 18 is directed via objective and eyepiece 17 to image plane 30. An image analysis may be initiated by detection of the presence of a cell at plane 18 by capacitor 35, which may also be used to sense cell size or volume, and inhibit analysis if the cell is too small or too large (e.g. a clump of cells). Image analysis is by an array of fibre optic guides 40 leading to corresponding photo-detectors 42, connected to respective amplifiers 43, amplitude discriminators 44a, 44b &c., which may classify the light intensities into e.g. four levels of grey (background, cytoplasm, normal nucleus, cancerous nucleus). A circuit 45 is used to determine characteristics of the cell from the discriminator outputs. A count or summing of spots of a particular intensity may be used to determine the area of a nucleus, cytoplasm &c. Display of counts on a C.R.T. is suggested. In dependence on an evaluation of the cell at plane 18, it may be diverted from the normal direction (along conduit 34) to conduit 31, by opening valve 32. An alternative sorting arrangement comprises altering the charge on collar 51, so that droplets of liquid formed by means of Piezoelectric vibrator 47 are deflected by plates 52 into different receptacles 53-55. In an alternative arrangement, a linear array of optical fibres monitors passage of a cell moving parallel to the optical axis, so as to generate a sequence of signals which provide indications of texture density and shape of parts of the cell. In the embodiment of Fig.3, a linear array of optical fibres is provided to allow investigation of a cell moving along the focal plane, the sample being carried on a glass slide 60 or transparent tape. The output ends of the the fibres are connected to an arrangement of three dichroic mirrors and associated photo-cells for colour analysis of the cell. In the embodiment of Fig.4, inlet and outlet conduits lead a sample flow in and out of the microscope substantially at right angles to the optical axis. The apparatus includes platinum electrodes 75,76 to which a D.C. supply is connected, to operate on the Coulter principle and allow volume sensing of cells, and initiation of the optical measurement by an array of detectors 42 e.g. in an integrated circuit. The optical system of Fig.4 is modified in the arrangement of Fig.5 (not shown) in which sample enters along the optical axis. In the embodiment of Fig.7, a sample is fed from container 85, via valve 88, into the microscope and cells pass through a focal plane aperture defined by knife edges, Fig.8 (not shown). A first sizing test is carried out by means of a device applying an electric field or, as shown, rods 117 applying a magnetic field, associated with pick-up electrodes 121. The output is amplitude discriminated by 127, 128 operating on valves 102, 103 to define a size range outside which cells pass to container 90. To decide which of 95 and 96 a cell is diverted to, the photo-electric system of three photo-detectors 42, viewing through sector apertures, includes an anti-coincidence circuit and further pulse height discriminators. The optical system for this embodiment is detailed in Fig.6 (not shown) but may be modified as detailed in Fig.9 (not shown), in which illumination by direct or tangential light is provided. The light source for the peripheral (tangential) lens system may be operated independently of that for the axial (direct) system, under the control of the photo-detectors. Particles for analysis may be suspended in air. Besides absorption and reflection, fluorescence measurements may be made. The number of cells of certain types (red, white &c.) or sizes may be counted, e.g. in Fig.7, by connecting counters to amplifiers 130, 131.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2406272A GB1389553A (en) | 1972-05-23 | 1972-05-23 | Apparatus for investigating microscopic particles |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2406272A GB1389553A (en) | 1972-05-23 | 1972-05-23 | Apparatus for investigating microscopic particles |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1389553A true GB1389553A (en) | 1975-04-03 |
Family
ID=10205763
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2406272A Expired GB1389553A (en) | 1972-05-23 | 1972-05-23 | Apparatus for investigating microscopic particles |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB1389553A (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2818842A1 (en) * | 1977-04-30 | 1978-11-09 | Olympus Optical Co | DEVICE FOR AUTOMATICALLY DETECTING CELLS |
DE2844004A1 (en) * | 1978-10-09 | 1980-04-17 | Cubana Export Import | Double microscope for observing fluid samples - uses capillary tubes in objective planes and fluid driven sample positioning |
GB2000286B (en) * | 1977-06-25 | 1982-04-21 | Pfister Waagen Gmbh | A method of ascertaining a characteristic or characteristics of a test object formed of butchers meat |
DE3208447A1 (en) * | 1981-03-09 | 1982-09-23 | Siemens AG, 1000 Berlin und 8000 München | Colour-modulated fibre optic transducer |
GB2152660A (en) * | 1983-11-28 | 1985-08-07 | Cii | Analysis of biological products |
EP0177718A2 (en) * | 1984-09-11 | 1986-04-16 | Partec AG | Method and device for sorting microscopic particles |
EP1651947A1 (en) * | 2003-07-19 | 2006-05-03 | DIGITAL BIO TECHNOLOGY Seoul National University Institute of Advance | Device for counting micro particles |
WO2009022152A1 (en) * | 2007-08-16 | 2009-02-19 | Imperial Innovations Limited | Single molecule spectroscopy using nanoporous membranes |
CN102221548A (en) * | 2010-04-13 | 2011-10-19 | 浙江海洋学院 | Analysis method for chemical staining surface of lymph caryons |
FR2970334A1 (en) * | 2011-01-07 | 2012-07-13 | Horiba Abx Sas | DEVICE FOR INSPECTING A BIOLOGICAL FLUID |
CN105717044A (en) * | 2016-03-30 | 2016-06-29 | 绵阳祥泰科技有限责任公司 | Laser based plasticizer detection device |
ES2599056A1 (en) * | 2015-07-31 | 2017-01-31 | Pablo ALBERTOS SÁNCHEZ | Device for testing a sample of cells (Machine-translation by Google Translate, not legally binding) |
CN110108604A (en) * | 2019-05-17 | 2019-08-09 | 成都信息工程大学 | High-altitude particle device for identifying and method based on micro- amplification and visual angle sensing |
CN110220836A (en) * | 2019-06-26 | 2019-09-10 | 袁家彬 | A kind of vitro detection stream type cell analyzer |
CN111656247A (en) * | 2018-07-13 | 2020-09-11 | 深圳迈瑞生物医疗电子股份有限公司 | Cell image processing system, cell image processing method, automatic film reading device and storage medium |
-
1972
- 1972-05-23 GB GB2406272A patent/GB1389553A/en not_active Expired
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2818842A1 (en) * | 1977-04-30 | 1978-11-09 | Olympus Optical Co | DEVICE FOR AUTOMATICALLY DETECTING CELLS |
GB2000286B (en) * | 1977-06-25 | 1982-04-21 | Pfister Waagen Gmbh | A method of ascertaining a characteristic or characteristics of a test object formed of butchers meat |
DE2844004A1 (en) * | 1978-10-09 | 1980-04-17 | Cubana Export Import | Double microscope for observing fluid samples - uses capillary tubes in objective planes and fluid driven sample positioning |
DE3208447A1 (en) * | 1981-03-09 | 1982-09-23 | Siemens AG, 1000 Berlin und 8000 München | Colour-modulated fibre optic transducer |
GB2152660A (en) * | 1983-11-28 | 1985-08-07 | Cii | Analysis of biological products |
EP0177718A2 (en) * | 1984-09-11 | 1986-04-16 | Partec AG | Method and device for sorting microscopic particles |
EP0177718A3 (en) * | 1984-09-11 | 1987-05-20 | Partec Ag | Method and device for sorting microscopic particles |
EP1651947A1 (en) * | 2003-07-19 | 2006-05-03 | DIGITAL BIO TECHNOLOGY Seoul National University Institute of Advance | Device for counting micro particles |
EP1651947A4 (en) * | 2003-07-19 | 2010-04-21 | Digital Bio Technology Seoul N | Device for counting micro particles |
WO2009022152A1 (en) * | 2007-08-16 | 2009-02-19 | Imperial Innovations Limited | Single molecule spectroscopy using nanoporous membranes |
CN102221548A (en) * | 2010-04-13 | 2011-10-19 | 浙江海洋学院 | Analysis method for chemical staining surface of lymph caryons |
CN102221548B (en) * | 2010-04-13 | 2012-11-14 | 浙江海洋学院 | Analysis method for chemical staining surface of lymph caryons |
FR2970334A1 (en) * | 2011-01-07 | 2012-07-13 | Horiba Abx Sas | DEVICE FOR INSPECTING A BIOLOGICAL FLUID |
ES2599056A1 (en) * | 2015-07-31 | 2017-01-31 | Pablo ALBERTOS SÁNCHEZ | Device for testing a sample of cells (Machine-translation by Google Translate, not legally binding) |
CN105717044A (en) * | 2016-03-30 | 2016-06-29 | 绵阳祥泰科技有限责任公司 | Laser based plasticizer detection device |
CN111656247A (en) * | 2018-07-13 | 2020-09-11 | 深圳迈瑞生物医疗电子股份有限公司 | Cell image processing system, cell image processing method, automatic film reading device and storage medium |
CN110108604A (en) * | 2019-05-17 | 2019-08-09 | 成都信息工程大学 | High-altitude particle device for identifying and method based on micro- amplification and visual angle sensing |
CN110108604B (en) * | 2019-05-17 | 2024-06-14 | 成都信息工程大学 | High-altitude particle identification device and method based on microscopic amplification and visual angle sensing |
CN110220836A (en) * | 2019-06-26 | 2019-09-10 | 袁家彬 | A kind of vitro detection stream type cell analyzer |
CN110220836B (en) * | 2019-06-26 | 2021-12-21 | 国科赛赋(深圳)新药研发科技有限公司 | Flow cytometry analyzer for in-vitro detection |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed [section 19, patents act 1949] | ||
PCNP | Patent ceased through non-payment of renewal fee |