GB1344340A - Method for fractionating plasma proteins - Google Patents

Method for fractionating plasma proteins

Info

Publication number
GB1344340A
GB1344340A GB1784271A GB1784271A GB1344340A GB 1344340 A GB1344340 A GB 1344340A GB 1784271 A GB1784271 A GB 1784271A GB 1784271 A GB1784271 A GB 1784271A GB 1344340 A GB1344340 A GB 1344340A
Authority
GB
United Kingdom
Prior art keywords
solution
precipitate
igg
plasma
globulins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1784271A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Statens Bakteriologiska Lab
Original Assignee
Statens Bakteriologiska Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Statens Bakteriologiska Lab filed Critical Statens Bakteriologiska Lab
Publication of GB1344340A publication Critical patent/GB1344340A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Abstract

1344340 Plasma fractionation STATENS BAKTERIOLOGISKA LABORATORIUM 28 May 1971 [2 June 1970] 17842/71 Heading A5B Blood is fractionated, especially to produce IgG, by the following steps: a) removal of corpuscles and other particles to give plasma, b) precipitating globulins from the plasma by addition of polyethylene glycol (M.W. c. 6000) to a concentration of 10-15% by weight of pH 6.5-8.0, c) removal of the precipitate and, if desired, extraction of albumin from the remaining solution, d) extracting the precipitate with 0.05M sodium acetate/acetic acid buffer at pH 5.8 (IgG dissolved, IgM and fibrinozin remain undissolved and are removed), e) adsorbing the solution from d) on a cation exchanger (e.g. carboxy methyl dextran) at pH 5.8 and eluting with 0.2M NaCl in 0.05 phosphate buffer at pH 7.5-8.0, f) reprecipitating the eluate with polyethylene glycol at pH 6.5-8.0 and dissolving the precipitate in 0.2M phosphate buffer at pH 6.6, g) adsorbing all the globulins other than IgG from the solution from f) on an anion exchanger, e.g. DEAE- dextran at pH 6.6, h) cooling the resulting IgG solution to -0.3‹C and precipitating with ethanol (25% concentration) at -5 to -10‹C, i) separating and suspending the precipitate and washing it with 25% ethanol at pH 7.0 at -5 to -10‹C, j) removing solids by centrifugation and dissolving in glycine, k) sterilefiltering the solution and freeze-drying.
GB1784271A 1970-06-02 1971-05-28 Method for fractionating plasma proteins Expired GB1344340A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE763270A SE348942B (en) 1970-06-02 1970-06-02

Publications (1)

Publication Number Publication Date
GB1344340A true GB1344340A (en) 1974-01-23

Family

ID=20271889

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1784271A Expired GB1344340A (en) 1970-06-02 1971-05-28 Method for fractionating plasma proteins

Country Status (8)

Country Link
AT (1) AT309678B (en)
CA (1) CA960964A (en)
CH (1) CH572745A5 (en)
DE (1) DE2127159C2 (en)
FR (1) FR2100716B1 (en)
GB (1) GB1344340A (en)
NL (1) NL177599C (en)
SE (1) SE348942B (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010082894A1 (en) * 2009-01-13 2010-07-22 Ge Healthcare Bio-Sciences Ab Precipitation of biomolecules with negatively charged polymers
ITFI20090273A1 (en) * 2009-12-28 2011-06-29 Kedrion Spa NEW PROCESS OF PURIFICATION ON INDUSTRIAL SCALE OF GAMMAGLOBULINE FROM HUMAN PLASMA FOR INDUSTRIAL USE.
US8546548B2 (en) 2009-05-27 2013-10-01 Baxter International Inc. Method to produce a highly concentrated immunoglobulin preparation for subcutaneous use
US8772462B2 (en) 2010-05-26 2014-07-08 Baxter International Inc. Removal of serine proteases by treatment with finely divided silicon dioxide
US8796430B2 (en) 2010-05-26 2014-08-05 Baxter International Inc. Method to produce an immunoglobulin preparation with improved yield
US8940877B2 (en) 2010-05-26 2015-01-27 Baxter International Inc. Method to produce an immunoglobulin preparation with improved yield
US9403899B2 (en) 2011-08-26 2016-08-02 Baxalta Incorporated Method for reducing the thromboembolic potential of a plasma-derived immunoglobulin composition
US9782477B2 (en) 2012-02-23 2017-10-10 Baxalta Incorporated Fraction I-IV-1 precipitation of immunoglobins from plasma

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE389596B (en) * 1974-06-14 1976-11-15 P G S Lindroos SUGGESTED TO SEPARATE GLOBIN IRON COMPOUNDS FROM A VETICAL CONTAINING BLOOD SUBSTANCES
DE2500076C3 (en) * 1975-01-02 1982-11-18 SCHURA Blutderivate GmbH & Co KG, 4150 Krefeld Process for the production of intravenously tolerated gamma globulins
US4124576A (en) * 1976-12-03 1978-11-07 Coval M L Method of producing intravenously injectable gamma globulin
AT359641B (en) * 1978-09-19 1980-11-25 Immuno Ag METHOD FOR PRODUCING AN INTRAVENOUS ADMINISTRABLE ANTIBODY-CONTAINING IMMUNOGLOBULIN PREPARATE
US4315851A (en) * 1978-12-29 1982-02-16 Kureha Kagaku Kogyo Kabushiki Kaisha Pharmaceutical composition having antitumor activity
EA201892521A1 (en) 2012-02-29 2019-07-31 Баксалта Инкорпорейтид REMYELINIZATION OF PERIPHERAL NERVES, STIMULATED IgG
EP2961426B1 (en) 2013-02-26 2019-10-30 Baxalta GmbH Treatment of central nervous system disorders by intranasal administration of immunoglobulin g
LT2994160T (en) 2013-05-06 2019-08-26 Baxalta Incorporated Treatment of alzheimer`s disease subpopulations with pooled immunoglobulin g
TW202342522A (en) 2022-03-07 2023-11-01 日商武田藥品工業股份有限公司 Affinity chromatographic production of clinical human igg products

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL286954A (en) * 1962-01-03
BE713764A (en) * 1967-05-01 1968-09-16 Baxter Travenol Lab
AT275038B (en) * 1967-11-15 1969-10-10 Immuno Ag Process for the production of pure blood albumin from protein solutions containing hemopigments

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010082894A1 (en) * 2009-01-13 2010-07-22 Ge Healthcare Bio-Sciences Ab Precipitation of biomolecules with negatively charged polymers
US8546548B2 (en) 2009-05-27 2013-10-01 Baxter International Inc. Method to produce a highly concentrated immunoglobulin preparation for subcutaneous use
US11242380B2 (en) 2009-05-27 2022-02-08 Takeda Pharmaceutical Company Limited Method to produce a highly concentrated immunoglobulin preparation for subcutaneous use
US10125189B2 (en) 2009-05-27 2018-11-13 Baxalta Incorporated Method to produce a highly concentrated immunoglobulin preparation for subcutaneous use
US9175068B2 (en) 2009-05-27 2015-11-03 Baxalta Incorporated Method to produce a highly concentrated immunoglobulin preparation for subcutaneous use
US8933204B2 (en) 2009-12-28 2015-01-13 Kedrion S.P.A. Process for the industrial-scale purification of gamma globulins from human plasma for industrial applications
ITFI20090273A1 (en) * 2009-12-28 2011-06-29 Kedrion Spa NEW PROCESS OF PURIFICATION ON INDUSTRIAL SCALE OF GAMMAGLOBULINE FROM HUMAN PLASMA FOR INDUSTRIAL USE.
WO2011080698A1 (en) * 2009-12-28 2011-07-07 Kedrion S.P.A. Process for the industrial - scale purification of gamma globulins from human plasma for industrial applications
AU2010337871B2 (en) * 2009-12-28 2013-05-09 Kedrion S.P.A. Process for the industrial - scale purification of gamma globulins from human plasma for industrial applications
US8940877B2 (en) 2010-05-26 2015-01-27 Baxter International Inc. Method to produce an immunoglobulin preparation with improved yield
US8772462B2 (en) 2010-05-26 2014-07-08 Baxter International Inc. Removal of serine proteases by treatment with finely divided silicon dioxide
US8993734B2 (en) 2010-05-26 2015-03-31 Baxter International Inc. Method to produce an immunoglobulin preparation with improved yield
US8889838B2 (en) 2010-05-26 2014-11-18 Baxter International Inc. Removal of serine proteases by treatment with finely divided silicon dioxide
US8921520B2 (en) 2010-05-26 2014-12-30 Baxter International Inc. Removal of serine proteases by treatment with finely divided silicon dioxide
US9468675B2 (en) 2010-05-26 2016-10-18 Baxalta Incorporated Removal of serine proteases by treatment with finely divided silicon dioxide
US9708391B2 (en) 2010-05-26 2017-07-18 Baxalta Incorporated Removal of serine proteases by treatment with finely divided silicon dioxide
US11891431B2 (en) 2010-05-26 2024-02-06 Takeda Pharm Limited ceutical Company Limited Removal of serine proteases by treatment with finely divided silicon dioxide
US8796430B2 (en) 2010-05-26 2014-08-05 Baxter International Inc. Method to produce an immunoglobulin preparation with improved yield
US10208106B2 (en) 2010-05-26 2019-02-19 Baxalta Incorporated Removal of serine proteases by treatment with finely divided silicon dioxide
US10875906B2 (en) 2010-05-26 2020-12-29 Baxalta Incorporated Removal of serine proteases by treatment with finely divided silicon dioxide
US11136350B2 (en) 2010-05-26 2021-10-05 Takeda Pharmaceutical Company Limited Method to produce an immunoglobulin preparation with improved yield
US9403899B2 (en) 2011-08-26 2016-08-02 Baxalta Incorporated Method for reducing the thromboembolic potential of a plasma-derived immunoglobulin composition
US9782477B2 (en) 2012-02-23 2017-10-10 Baxalta Incorporated Fraction I-IV-1 precipitation of immunoglobins from plasma

Also Published As

Publication number Publication date
NL7107529A (en) 1971-12-06
FR2100716B1 (en) 1975-06-06
FR2100716A1 (en) 1972-03-24
DE2127159C2 (en) 1981-12-24
AT309678B (en) 1973-08-27
NL177599B (en) 1985-05-17
DE2127159A1 (en) 1971-12-09
SE348942B (en) 1972-09-18
CH572745A5 (en) 1976-02-27
CA960964A (en) 1975-01-14
NL177599C (en) 1985-10-16

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Legal Events

Date Code Title Description
PS Patent sealed
PE20 Patent expired after termination of 20 years