GB1314263A - Blood diagnostic methods employing radioactive tracer compounds and ion-exchange resin membranes - Google Patents

Blood diagnostic methods employing radioactive tracer compounds and ion-exchange resin membranes

Info

Publication number
GB1314263A
GB1314263A GB1939170A GB1939170A GB1314263A GB 1314263 A GB1314263 A GB 1314263A GB 1939170 A GB1939170 A GB 1939170A GB 1939170 A GB1939170 A GB 1939170A GB 1314263 A GB1314263 A GB 1314263A
Authority
GB
United Kingdom
Prior art keywords
membrane
thyroxine
serum
exchange resin
serum protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1939170A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mallinckrodt Chemical Works
Original Assignee
Mallinckrodt Chemical Works
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mallinckrodt Chemical Works filed Critical Mallinckrodt Chemical Works
Publication of GB1314263A publication Critical patent/GB1314263A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
    • G21H—OBTAINING ENERGY FROM RADIOACTIVE SOURCES; APPLICATIONS OF RADIATION FROM RADIOACTIVE SOURCES, NOT OTHERWISE PROVIDED FOR; UTILISING COSMIC RADIATION
    • G21H5/00—Applications of radiation from radioactive sources or arrangements therefor, not otherwise provided for 
    • G21H5/02—Applications of radiation from radioactive sources or arrangements therefor, not otherwise provided for  as tracers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/825—Pretreatment for removal of interfering factors from sample
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/826—Additives, e.g. buffers, diluents, preservatives

Abstract

1314263 Determination of blood components MALLINCKRODT CHEMICAL WORKS 22 April 1970 [1 Ma,y 1969 13 Oct 1969] 19391/70 Heading BIX Blood components which can be bound by serum protein, for example thyroxine or triiodothyronine, or iron are determined by a method comprising preparing a liquid sample containing blood serum protein and a predetermined amount of a radioactive tracer compound capable of being bound by the serum protein and by an ion exchange resin, adding to the sample a thin strip of an ion exchange resin membrane and maintaining sample and strip in contact for a predetermined time, separating the membrane from the sample and determining the relative amounts of the labelled compound bound to the serum protein in the sample and to the membrane. The blood serum protein may be globulin, albumin or prealbumin. The radioactively labelled compound may be I<SP>125</SP>-thyroxine or I<SP>131</SP>-L-triiodothyronine for determination of the total thyroxine content of serum or the throxine binding capacity of serum protein, or ferric (Fe<SP>59</SP>) ammonium citrate for determination of the iron-binding capacity of serum proteins. The membrane may be a strip, sheet or film of solid hydrous gel of thickness 5 mils to 50 mls and may be comprise anionic or cationic exchange resin. Exemplified is a membrane comprising a vinyl copolymer carrying quaternary ammonium groups, the membrane being reinforced with loosely woven fibres of a co-polymer of vinyl chloride and acrylonitrile. A reagent for use in the determination of thyroxine content is prepared by diluting blood serum with barbital buffer pH 8.6, passing the diluted serum through an anion exchange column, which adsorbs about 80% of the thyroxine, and adding radioactive thyroxine to the filtered eluate.
GB1939170A 1969-05-01 1970-04-22 Blood diagnostic methods employing radioactive tracer compounds and ion-exchange resin membranes Expired GB1314263A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82109769A 1969-05-01 1969-05-01
US86578569A 1969-10-13 1969-10-13

Publications (1)

Publication Number Publication Date
GB1314263A true GB1314263A (en) 1973-04-18

Family

ID=27124511

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1939170A Expired GB1314263A (en) 1969-05-01 1970-04-22 Blood diagnostic methods employing radioactive tracer compounds and ion-exchange resin membranes

Country Status (11)

Country Link
US (1) US3714344A (en)
JP (2) JPS5617620B1 (en)
BE (1) BE749772A (en)
CH (1) CH526781A (en)
DE (1) DE2021968A1 (en)
FR (1) FR2047211A5 (en)
GB (1) GB1314263A (en)
IL (1) IL34387A (en)
IT (1) IT943545B (en)
NL (2) NL7006455A (en)
SE (1) SE378679B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4289748A (en) * 1979-05-31 1981-09-15 United States Of America Ultrasensitive enzymatic radioimmunoassay method
US11768689B2 (en) 2013-08-08 2023-09-26 Movidius Limited Apparatus, systems, and methods for low power computational imaging

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3911096A (en) * 1972-06-23 1975-10-07 Professional Staff Ass Of The Radioimmunoassay for measurement of thyroxine (T{HD 4{B ) and triiodothyonine (T{HD 3{B ) in blood serum
USRE32098E (en) * 1972-06-23 1986-03-25 Research And Education Institute, Inc. Radioimmunoassay for measurement of thyroxine (T4) and triiodothyronine (T3) in blood serum
US3966897A (en) * 1973-04-02 1976-06-29 Marine Colloids, Inc. Medium for use in bioassay and method of using same
GB1412274A (en) * 1973-05-29 1975-11-05 Bio Rad Laboratories Radioactive determination of serum thyroxine
US3929981A (en) * 1974-02-27 1975-12-30 Squibb & Sons Inc Method for determining thyroid function
NL7605447A (en) * 1975-06-26 1976-12-28 Byk Mallinckrodt Chem Prod RADIOIMMUNO TEST METHOD FOR THE DETERMINATION OF THYROXIN.
US4282002A (en) * 1979-09-06 1981-08-04 Akzona Incorporated Sensitized sheep stroma immunoassay for rheumatoid factor
US6242261B1 (en) 1992-02-10 2001-06-05 University Of Saskatchewan Assessment of ion availability in heterogeneous media using ion-exchange membranes
EP0649533B1 (en) * 1992-03-30 2000-05-10 Abbott Laboratories Reagents and methods for the detection and quantification of thyroxine in fluid samples
US5352803A (en) * 1992-03-30 1994-10-04 Abbott Laboratories 5(6)-methyl substituted fluorescein derivatives
FR2878333B1 (en) * 2004-11-25 2007-04-27 Cie Gen De S Eaux Soc En Comma CASE FOR COLLECTING WATER AND CAPTURING ODOR-PRODUCING SUBSTANCES AND / OR GOUTS IN THE WATER

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3414383A (en) * 1965-08-09 1968-12-03 Canadian Patents Dev Determination of thyroxine

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4289748A (en) * 1979-05-31 1981-09-15 United States Of America Ultrasensitive enzymatic radioimmunoassay method
US11768689B2 (en) 2013-08-08 2023-09-26 Movidius Limited Apparatus, systems, and methods for low power computational imaging

Also Published As

Publication number Publication date
NL7006455A (en) 1970-11-03
IT943545B (en) 1973-04-10
NL7417037A (en) 1975-04-29
BE749772A (en) 1970-10-30
IL34387A0 (en) 1970-06-17
JPS5542704B1 (en) 1980-11-01
SE378679B (en) 1975-09-08
US3714344A (en) 1973-01-30
JPS5617620B1 (en) 1981-04-23
DE2021968A1 (en) 1970-11-05
CH526781A (en) 1972-08-15
FR2047211A5 (en) 1971-03-12
IL34387A (en) 1973-04-30

Similar Documents

Publication Publication Date Title
Benson Jr et al. Accelerated chromatographic analysis of amino acids in physiological fluids containing glutamine and asparagine
GB1314263A (en) Blood diagnostic methods employing radioactive tracer compounds and ion-exchange resin membranes
Inczédy Analytical applications of ion exchangers
US4314823A (en) Combination apparatus and method for chromatographic separation and quantitative analysis of multiple ionic species
Pensky et al. Studies on thyroxine-binding globulin (TBG): II. Separation from human serum by affinity chromatagraphy
Westerlund Direct injection of plasma into column liquid chromatographic systems
GB1564580A (en) Specific binding assay method and test kit employing polystyrene as separating agent
Buchanan et al. Impregnated silicone rubber membranes for potential-indicating electrodes
US3592888A (en) Radioimmunoassay of angiotensin and renin activity
Boyd et al. Human placental sulphate transport: studies on chorionic trophoblast brush border membrane vesicles.
ES346273A1 (en) Method for determining vitamin b12 and reagent therefor
Ktari et al. Simplified flux equation in Donnan dialysis
CUARÓN Relationship between the in vitro uptake of 131I-triiodothyronine by erythrocytes and its binding by serum proteins in thyroid disease
GB1412274A (en) Radioactive determination of serum thyroxine
GB1347498A (en) Test compositions for the determination of thyroid function
CA1175351A (en) Reusable assay for multisite antigens
Hirayama et al. Novel separation of inorganic anions using a charged complex ion-exchanger
Krøll Immunoelectrophoretic quantitation of trace proteins
Miura et al. Determination of thiocyanate in human urine samples by suppressed ion chromatography
Ahluwalia et al. Determination of cerium in ferrous alloys
Boardman Ion-exchange chromatography
Foss I131 As an “Amplifier” in Paper Electrophoresis of Protein Solutions of Low Concentration
Shimura et al. Influence of a soluble anionic polymer on the electrophoresis of proteins
Tuckerman Use of Ion Exchange Paper in Analytical Chemistry
Cook et al. Saturation in the competitive protein-binding assay of serum thyroxine

Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee