GB1245411A - Improvements in and relating to microtome assemblies - Google Patents

Improvements in and relating to microtome assemblies

Info

Publication number
GB1245411A
GB1245411A GB787368A GB787368A GB1245411A GB 1245411 A GB1245411 A GB 1245411A GB 787368 A GB787368 A GB 787368A GB 787368 A GB787368 A GB 787368A GB 1245411 A GB1245411 A GB 1245411A
Authority
GB
United Kingdom
Prior art keywords
blade
solenoids
plunger
leaf
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB787368A
Inventor
Reginald Dawson Preston
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NGN Ltd
Original Assignee
NGN Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NGN Ltd filed Critical NGN Ltd
Priority to GB787368A priority Critical patent/GB1245411A/en
Publication of GB1245411A publication Critical patent/GB1245411A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • G01N2001/065—Drive details

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

1,245,411. Microtomes. N. G. N. Ltd. Dec. 23, 1968 [Feb. 17, 1968], No. 7873/68. Heading G2J. In a microtome assembly, the knife is vertically adjustable and horizontally displaceable and its blade cooled below the temperature of the specimen to be cleared and thermally insulated from the remaining assembly. As shown, a knife body 18 pivotally suspended by pivot pins from arms 30, 32 of low thermal conductance has a blade 20 and is connected at its rear end via a low thermal conductance leaf-spring 28 to a plunger 26 displacing blade 20 along its cutting stroke, being actuated in a forward and reverse direction by either of two coaxially mounted solenoids 22, 24. Arms 30, 32 are connected by leaf-springs 34, 36 to a bracket support 38 rigidly attached to a control shaft 40 central of a bearing 42 and passing through an O-ring seal in base-plate 10 to a micrometer adjusting screw and gear-box (not shown) to effect the vertical adjustment. Pipes 44, 46 adjustable horizontally and vertically by means of bellows 48, 50 deliver a coolant such as liquid nitrogen to the blade 20. The assembly may be housed in a vacuum chamber the solenoids either being located outside the chamber and the plunger passing through a bore in the wall thereof, both provided within the chamber, or the solenoid wrapped round a tubular extension thereof, the plunger moving within this extension. In a further embodiment, Fig. 7, (not shown) the solenoids (22, 24) are enclosed within a vessel (70) connected to the vacuum chamber having an axial bore through which the plunger (26) passes and is connected via a tube (72) to atmosphere to permit electrical connections to be passed to the solenoids (22, 24) and to remove contamination within the vacuum system. An arrangement is described for lifting the blade 22 clear of the specimen 14 on its return stroke comprising pressing the forward pivot pin against the underneath and top side of a pair of inclined leaf-springs on its forward and reverse strokes respectively.
GB787368A 1968-09-17 1968-09-17 Improvements in and relating to microtome assemblies Expired GB1245411A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB787368A GB1245411A (en) 1968-09-17 1968-09-17 Improvements in and relating to microtome assemblies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB787368A GB1245411A (en) 1968-09-17 1968-09-17 Improvements in and relating to microtome assemblies

Publications (1)

Publication Number Publication Date
GB1245411A true GB1245411A (en) 1971-09-08

Family

ID=9841423

Family Applications (1)

Application Number Title Priority Date Filing Date
GB787368A Expired GB1245411A (en) 1968-09-17 1968-09-17 Improvements in and relating to microtome assemblies

Country Status (1)

Country Link
GB (1) GB1245411A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2147119A (en) * 1983-09-27 1985-05-01 Parke Davis & Co Microtome having hand rests
GB2154022A (en) * 1984-02-07 1985-08-29 Parke Davis & Co Microtome having specimen retraction means
DE102014118801A1 (en) * 2014-12-17 2016-06-23 Leica Mikrosysteme Gmbh Freeze-fracture machine
CN111389322A (en) * 2020-03-31 2020-07-10 华北水利水电大学 Hydrothermal-heat treatment automatic experiment platform

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2147119A (en) * 1983-09-27 1985-05-01 Parke Davis & Co Microtome having hand rests
GB2154022A (en) * 1984-02-07 1985-08-29 Parke Davis & Co Microtome having specimen retraction means
DE102014118801A1 (en) * 2014-12-17 2016-06-23 Leica Mikrosysteme Gmbh Freeze-fracture machine
JP2016114609A (en) * 2014-12-17 2016-06-23 ライカ ミクロジュステーメ ゲーエムベーハー Freeze-fracture device
DE102014118801B4 (en) * 2014-12-17 2016-10-06 Leica Mikrosysteme Gmbh Freeze-fracture machine
US10073016B2 (en) 2014-12-17 2018-09-11 Leica Mikrosysteme Gmbh Freeze fracture machine
CN111389322A (en) * 2020-03-31 2020-07-10 华北水利水电大学 Hydrothermal-heat treatment automatic experiment platform

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Legal Events

Date Code Title Description
PS Patent sealed
PLNP Patent lapsed through nonpayment of renewal fees