GB1214891A - Improved process for preparing thyrocalcitonin - Google Patents

Improved process for preparing thyrocalcitonin

Info

Publication number
GB1214891A
GB1214891A GB00112/68A GB1011268A GB1214891A GB 1214891 A GB1214891 A GB 1214891A GB 00112/68 A GB00112/68 A GB 00112/68A GB 1011268 A GB1011268 A GB 1011268A GB 1214891 A GB1214891 A GB 1214891A
Authority
GB
United Kingdom
Prior art keywords
extract
thyrocalcitonin
organic solvent
water
inactive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB00112/68A
Inventor
Edward Leonard Grinnan
Robert Lawrence Wolen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of GB1214891A publication Critical patent/GB1214891A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

1,214,891. Thyrocalcitonin. ELI LILLY & CO. 1 March, 1968 [2 March, 1967], No. 10112/68. Heading A5B. Thyrocalcitonin having a significant serumcalcium-lowering activity in rats at a dose level of 5γ per kilo of rat body weight is prepared by extracting mammalian thyroid tissue with an acidic mixture of from 25 to 85 percent by volume of a water miscible organic solvent and water, e.g. acidic aqueousethanol, at a pH below 4, preferentially precipitating inactive proteinaceous materials from the extract by the addition of 1 to 3 volumes of watermiscible organic solvent per volume of extract or by adjusting the pH of the extract to about 6.5, separating the precipitated inactive proteinaceous material from the extract, precipitating thyroalcitonin from the extract by the addition of 5 to 10 volumes of a watermiscible organic solvent per volume of extract, and finally separating and purifying the thyrocalcitonin thus precipitated. The water-miscible solvent may be dioxane, dimethylformamide, acetone, methanol, isopropanol or ethanol and the acid may comprise hydrochloric, phosphoric, formic, acetic or sulphuric acid. The thyrocalcitonin may be purified by gel filtration or chromatography over a polydextran, optionally followed by lyophilization.
GB00112/68A 1967-03-02 1968-03-01 Improved process for preparing thyrocalcitonin Expired GB1214891A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US61991067A 1967-03-02 1967-03-02

Publications (1)

Publication Number Publication Date
GB1214891A true GB1214891A (en) 1970-12-09

Family

ID=24483828

Family Applications (1)

Application Number Title Priority Date Filing Date
GB00112/68A Expired GB1214891A (en) 1967-03-02 1968-03-01 Improved process for preparing thyrocalcitonin

Country Status (6)

Country Link
BE (1) BE711523A (en)
DE (1) DE1667878A1 (en)
DK (1) DK118363B (en)
FR (1) FR1556030A (en)
GB (1) GB1214891A (en)
NL (1) NL6802786A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3966224A4 (en) * 2019-05-06 2023-08-23 The University of Sydney Methods for the fractionation of proteins

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3966224A4 (en) * 2019-05-06 2023-08-23 The University of Sydney Methods for the fractionation of proteins

Also Published As

Publication number Publication date
DK118363B (en) 1970-08-10
NL6802786A (en) 1968-09-03
DE1667878A1 (en) 1971-07-29
FR1556030A (en) 1969-01-31
BE711523A (en) 1968-09-02

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