GB1199565A - Improvements relating to a Method of and Apparatus for Determining the Concentration in Solution of a Substance. - Google Patents

Improvements relating to a Method of and Apparatus for Determining the Concentration in Solution of a Substance.

Info

Publication number
GB1199565A
GB1199565A GB3562966A GB3562966A GB1199565A GB 1199565 A GB1199565 A GB 1199565A GB 3562966 A GB3562966 A GB 3562966A GB 3562966 A GB3562966 A GB 3562966A GB 1199565 A GB1199565 A GB 1199565A
Authority
GB
United Kingdom
Prior art keywords
oxygen
measured
concentration
solution
measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3562966A
Inventor
Eric Mitchell Crook
Derrick Arnold Patient
Paul Brohan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baird and Tatlock Ltd
Original Assignee
Baird and Tatlock Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baird and Tatlock Ltd filed Critical Baird and Tatlock Ltd
Priority to GB3562966A priority Critical patent/GB1199565A/en
Priority to DE19676609316 priority patent/DE6609316U/en
Publication of GB1199565A publication Critical patent/GB1199565A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Emergency Medicine (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1,199,565. Measuring oxygen content, electrochemically. BAIRD & TATLOCK (LONDON) Ltd. Aug.3, 1967 [Aug.9, 1966], No.35629/66. Heading G1N. The concentration of a substance in solution is measured by treating the solution with a reagent that reacts with the substance to vary the concentration of oxygen in the solution in proportion to the initial concentration of the substance and measuring the change or rate of change in the oxygen concentration using an oxygen electrode. As described with reference to Fig. 1, the reagent is introduced into chamber 1 through inlet 3, and may be pumped continuously through the cell for initially washing the chamber. The solution under test is added through an injecting lance 13. An electrolytic cell for measuring oxygen concentration comprising a platinum electrode 11, glass coated at 10, and silver/silver chloride reference anode 12 in a buffer solution containing phosphate and chloride ions is separated from the sample by an oxygen-permeable membrane 7. Stirring is by a magnet 15 on a plastic covered magnetizable slug 17. In an alternative arrangement, the membrane 7 is omitted so that the oxygen electrode is in direct contact with the solution under test. In this case the electrode is vibrated or rotated to maintain a continuously renewed contact between it and the surrounding liquid. Many examples of substances that can be measured and appropriate reagents are given. Glucose can be measured, using a 1% glucose oxidase solution saturated with air as the reagent, the slope of the recorded oxygen concentration being a measure of the concentration of glucose in the sample. If desired, a 0À1% alcohol or peroxidase and an aromatic amine or phenol may be added to the reagent to preserve stoichiometry in the presence of catalase. Galactoze, uric acid, ascorbic acid (vitamin C), amino acid, amine, diamine, xanthine, aldehyde, lipid and phenolic oxidases can be used as reagents or may be measured themselves. In general, any reaction which produces a molecule for which an oxidase or dehydrogenase exists can be measured by measuring oxygen consumption. Hydrogen peroxide can be measured, by catalytic conversion to water and oxygen. Catalase can be measured by measuring the rate of oxygen liberation from hydrogen peroxide or an alkyl hydroperoxide.
GB3562966A 1966-08-09 1966-08-09 Improvements relating to a Method of and Apparatus for Determining the Concentration in Solution of a Substance. Expired GB1199565A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB3562966A GB1199565A (en) 1966-08-09 1966-08-09 Improvements relating to a Method of and Apparatus for Determining the Concentration in Solution of a Substance.
DE19676609316 DE6609316U (en) 1966-08-09 1967-08-09 DEVICE FOR DETERMINING THE CONCENTRATION OF A SUBSTANCE IN SOLUTION.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3562966A GB1199565A (en) 1966-08-09 1966-08-09 Improvements relating to a Method of and Apparatus for Determining the Concentration in Solution of a Substance.

Publications (1)

Publication Number Publication Date
GB1199565A true GB1199565A (en) 1970-07-22

Family

ID=10379844

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3562966A Expired GB1199565A (en) 1966-08-09 1966-08-09 Improvements relating to a Method of and Apparatus for Determining the Concentration in Solution of a Substance.

Country Status (2)

Country Link
DE (1) DE6609316U (en)
GB (1) GB1199565A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2399666A1 (en) * 1977-08-03 1979-03-02 Philips Nv DEVICE FOR ENZYMATIC ANALYSIS
FR2401422A1 (en) * 1977-08-23 1979-03-23 Fresenius Chem Pharm Ind METHOD AND DEVICE FOR THE ELECTROCHEMICAL ENZYMATIC ANALYSIS
WO1981000725A1 (en) * 1979-09-14 1981-03-19 Charles Hospital Dev Method of determining a substrate in a sample
US4384041A (en) * 1980-04-07 1983-05-17 Toyo Jozo Kabushiki Kaisha Assay method of enzyme activity
US4446231A (en) * 1979-10-03 1984-05-01 Self Colin H Immunoassay using an amplified cyclic detection system
GB2273773A (en) * 1992-12-23 1994-06-29 Kodak Ltd Measuring hydrogen peroxide concentration
WO1997020215A1 (en) * 1995-11-28 1997-06-05 Ramot University Authority For Applied Research And Industrial Development Ltd. Detection of specific entities in a sample

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2399666A1 (en) * 1977-08-03 1979-03-02 Philips Nv DEVICE FOR ENZYMATIC ANALYSIS
FR2401422A1 (en) * 1977-08-23 1979-03-23 Fresenius Chem Pharm Ind METHOD AND DEVICE FOR THE ELECTROCHEMICAL ENZYMATIC ANALYSIS
WO1981000725A1 (en) * 1979-09-14 1981-03-19 Charles Hospital Dev Method of determining a substrate in a sample
US4446231A (en) * 1979-10-03 1984-05-01 Self Colin H Immunoassay using an amplified cyclic detection system
US4384041A (en) * 1980-04-07 1983-05-17 Toyo Jozo Kabushiki Kaisha Assay method of enzyme activity
GB2273773A (en) * 1992-12-23 1994-06-29 Kodak Ltd Measuring hydrogen peroxide concentration
GB2273773B (en) * 1992-12-23 1996-04-03 Kodak Ltd Method of determining hydrogen peroxide levels
WO1997020215A1 (en) * 1995-11-28 1997-06-05 Ramot University Authority For Applied Research And Industrial Development Ltd. Detection of specific entities in a sample

Also Published As

Publication number Publication date
DE6609316U (en) 1972-04-20

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Legal Events

Date Code Title Description
PS Patent sealed
PLNP Patent lapsed through nonpayment of renewal fees