GB1194256A - Immunological Indicator and Test System - Google Patents

Immunological Indicator and Test System

Info

Publication number
GB1194256A
GB1194256A GB2818167A GB2818167A GB1194256A GB 1194256 A GB1194256 A GB 1194256A GB 2818167 A GB2818167 A GB 2818167A GB 2818167 A GB2818167 A GB 2818167A GB 1194256 A GB1194256 A GB 1194256A
Authority
GB
United Kingdom
Prior art keywords
bdb
indicator
coli
hcg
tests
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2818167A
Inventor
Edward Robert Arquilla
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Priority to GB2818167A priority Critical patent/GB1194256A/en
Publication of GB1194256A publication Critical patent/GB1194256A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

1,194,256. Immunological indicator comprising microbial cells. MILES LABORATORIES Inc. 19 June, No. 28181/67. Heading B1X. An immunological indicator comprises microbial cells to which are attached coupling agent molecules by covalent bonds. The attached coupling agent molecules each have at least one non-bonded group which can form a covalent bond with antigens, giving an "indicator system". The microbial cells may be bacterial, fungal or protozoal cells with a maximum dimension of 0.2 to 10 microns, and may 'be treated with formaldehyde or phenol and optionally stained before use. The coupling agent may be bis diazobenzidine (BDB), bis diazobenzidine disulphonic acid, tetra-azo-pphenylene-diamine, difluorodinitrobenzene, a carbodiimide, toluene diisocyaniate, cyanuric chloride, dichloro-s-triazine, N-t-butyl-5-methyl -isoxazolium perchlorate. Indicator systems exemlified are Brucella abortus-BDB-HCG; E. coli-BDB-HCG Saccharomyces cerevisiae- BDB-HCG; E.Coli-N,N'-dicyclohexyl carbodiimide-HCG; E. coli-BDB-human serum albumin (HSA); E. coli-BDB-horse gamma globulin; E.coli-N-t-butyl-5-methylisoxazoliuma perchlorate-HSA; the same indicator coupled to horse gamma globulin; each of Bacillus subtilis, Bacillus pumilis, Lactobacillus leichmannii, Pseudomonas fragi coupled to BDB and to each of HSA, horse gamma globulin and human insulin; other components are mentioned. The indicator systems may be used in chromatography type tests, tube tests, slide or plate-tests. Test devices for chromatography type tests for antigens comprise bibulous materials carrying the residue resulting from either (a) the deposition of an indicator system as above, made from the antigen to be detected, and a superimposed deposition of the homologous antibody, or (b) the deposition of the immune aggregate formed from the indicator system and the homologous antibody. The device may be developed with a urine sample under test or a spot of the urine sample may be placed on the spot of immune aggregate and the device developed with saline. If the immune aggregate dissociates in the presence of the sample, the indicator system migrates, and if the cells were stained the migrating material forms coloured streaks.
GB2818167A 1967-06-19 1967-06-19 Immunological Indicator and Test System Expired GB1194256A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2818167A GB1194256A (en) 1967-06-19 1967-06-19 Immunological Indicator and Test System

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2818167A GB1194256A (en) 1967-06-19 1967-06-19 Immunological Indicator and Test System

Publications (1)

Publication Number Publication Date
GB1194256A true GB1194256A (en) 1970-06-10

Family

ID=10271560

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2818167A Expired GB1194256A (en) 1967-06-19 1967-06-19 Immunological Indicator and Test System

Country Status (1)

Country Link
GB (1) GB1194256A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2345461A1 (en) * 1976-03-25 1977-10-21 Dainippon Pharmaceutical Co INSOLUBILIZED ANTIBODIES SUITABLE FOR RADIO-IMMUNOLOGICAL OR ENZYMO-IMMUNOLOGICAL ANALYSIS AND ITS PROCESS FOR USE
US4235792A (en) * 1977-04-14 1980-11-25 The Governing Council Of The University Of Toronto Immunological materials
EP0613005A2 (en) * 1993-02-25 1994-08-31 Quidel Corporation Assays employing dyed microorganism labels
US5741662A (en) * 1995-12-18 1998-04-21 Quidel Corporation Direct stain specific binding assays for microorganisms
WO2016123165A2 (en) 2015-01-29 2016-08-04 Neogen Corporation Methods for immuno chromatographic assay desensitization

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2345461A1 (en) * 1976-03-25 1977-10-21 Dainippon Pharmaceutical Co INSOLUBILIZED ANTIBODIES SUITABLE FOR RADIO-IMMUNOLOGICAL OR ENZYMO-IMMUNOLOGICAL ANALYSIS AND ITS PROCESS FOR USE
US4235792A (en) * 1977-04-14 1980-11-25 The Governing Council Of The University Of Toronto Immunological materials
EP0613005A2 (en) * 1993-02-25 1994-08-31 Quidel Corporation Assays employing dyed microorganism labels
EP0613005A3 (en) * 1993-02-25 1995-07-05 Quidel Corp Assays employing dyed microorganism labels.
US5741662A (en) * 1995-12-18 1998-04-21 Quidel Corporation Direct stain specific binding assays for microorganisms
WO2016123165A2 (en) 2015-01-29 2016-08-04 Neogen Corporation Methods for immuno chromatographic assay desensitization

Similar Documents

Publication Publication Date Title
Smits et al. Immunochromatographic Brucella-specific immunoglobulin M and G lateral flow assays for rapid serodiagnosis of human brucellosis
Sakane et al. Protein A from Staphylococcus aureus—a mitogen for human T lymphocytes and B lymphocytes but not L lymphocytes
GB1490264A (en) Process for qualitative analysis or quantification of antigens or antibodies
NL182175C (en) METHOD FOR DETECTION AND QUANTITATIVE DETERMINATION OF ANTIGENS, ANTIBODIES OR ANTIBODY-ANTIGEN IMMUNE COMPLEXES IN BIOLOGICAL LIQUIDS.
SE7602834L (en) DIAGNOTIC REAGENT SYSTEM
ES8304314A1 (en) A method and a kit for the assay of antibodies to soluble antigens.
GB1248003A (en) Direct agglutination immunological reagent
JPS5516290A (en) Purified antigen for testing against neisseria gonorrhoeae antibody
ATE61735T1 (en) ANTIBODIES SPECIFIC FOR ENDOTOXINS.
GB1194256A (en) Immunological Indicator and Test System
CN103940990A (en) Sensitivity-enhanced gold-labeled immunochromatographic strip and preparation method thereof
GB1497327A (en) Direct radioimmunoassay for hepatitis associated antigens and their antibodies
ES8602253A1 (en) Monoclonal immunoglobulin M antibodies and method of preparation.
Kato et al. Use of antibody Fab′ fragments to remove interference by rheumatoid factors with the enzyme-linked sandwich immunoassay
Gerber et al. Cell-mediated immune response to hepatitis B antigen in patients with liver disease
NO883367D0 (en) CELL SURFACE ANTIGEN-detection systems.
Levy et al. Antimycobacterial antibody levels in pleural fluid as reflection of passive diffusion from serum
Bäck et al. Adverse reactions to nitrofurantoin in relation to cellular and humoral immune responses.
DE3686717D1 (en) IMMUNITY TEST PROCEDURE.
JP2017120193A (en) Strip for chromatography analysis
Min et al. Effect of prolonged incubation time on results of the QuantiFERON TB gold in-tube assay for diagnosis of latent tuberculosis infection
Chan et al. [21] Use of antibodies for studying the sidedness of membrane components
Maidment Jr et al. Isoelectric focusing analysis of soluble immune complexes bound to Protein A-Sepharose
SASAHIRA SERODIAGNOSIS OF SYPHILIS BY FLUORESCENT TREPONEMAL ANTIBODY TEST (FTA TEST). 2. ON THE TRANSITION OF ANTIBODIES OF 7 S GAMMA TYPE IN THE SYPHILITIC SERA IN EACH STAGE AND THE SPECIFICITY OF FTA TEST
ES2019573T3 (en) IMMUNO ASSAY FOR THE DETECTION OF ALPHA-HALOACETAMIDES.

Legal Events

Date Code Title Description
PS Patent sealed
PLNP Patent lapsed through nonpayment of renewal fees