GB1169183A - Apparatus for Density Gradient Electrophoresis - Google Patents
Apparatus for Density Gradient ElectrophoresisInfo
- Publication number
- GB1169183A GB1169183A GB44084/66A GB4408466A GB1169183A GB 1169183 A GB1169183 A GB 1169183A GB 44084/66 A GB44084/66 A GB 44084/66A GB 4408466 A GB4408466 A GB 4408466A GB 1169183 A GB1169183 A GB 1169183A
- Authority
- GB
- United Kingdom
- Prior art keywords
- tube
- density gradient
- trough
- covered
- openings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000001962 electrophoresis Methods 0.000 title abstract 3
- 239000007788 liquid Substances 0.000 abstract 5
- 239000012528 membrane Substances 0.000 abstract 4
- 239000000337 buffer salt Substances 0.000 abstract 2
- 238000001816 cooling Methods 0.000 abstract 2
- 238000005086 pumping Methods 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000012809 cooling fluid Substances 0.000 abstract 1
- 230000005684 electric field Effects 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 239000011810 insulating material Substances 0.000 abstract 1
- 230000031700 light absorption Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 210000002445 nipple Anatomy 0.000 abstract 1
- 239000010453 quartz Substances 0.000 abstract 1
- 230000002441 reversible effect Effects 0.000 abstract 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
- E21B43/20—Displacing by water
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/4476—Apparatus specially adapted therefor of the density gradient type
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Mining & Mineral Resources (AREA)
- Geology (AREA)
- Environmental & Geological Engineering (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Fluid Mechanics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geochemistry & Mineralogy (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Peptides Or Proteins (AREA)
- Electrostatic Separation (AREA)
Abstract
1,169,183. Density gradient electro-phoresis apparatus. INSTRUMENTATION SPECIALTIES CO. Oct.3, 1966 [May 25, 1966], No.44084/66. Heading C7B. Apparatus for density gradient electro-phoresis comprises a vertically positioned tube of electrical insulating material for receiving density gradient liquid, means including a reversible pump for pumping a displacing liquid into and out of the tube through the lower end thereof to vary the position of the density gradient liquid within the tube, and means establishing an electric field across axially-spaced points within the tube comprising electrodes connected to a source of D.C. voltage and immersed in buffer solutions contained in chambers having communication with the tube at the axially spaced points through openings covered by semi-permeable membranes. In the embodiment shown the tube for containing the density gradient liquid comprises tubes 11, 11' positioned end to end and separated by a quartz ring 21 at opposite sides of which are positioned a lamp 16 and photo-cell 24, respectively, forming a photo-metric scanning system. By pumping displacing liquid into and out from the tube via fitting 67 the fractionated zones of the sample may be passed by said scanning system and the light absorption, and hence the concentration of the fractionated zones, may be measured, which scanning process may be repeated as often as desired. The upper end of the tube 11 abuts a tube 27 having appertures 38 covered by semipermeable membranes, which appertures connect with chambers 31 and hence with trough 34, the chambers 31 and trough 34 containing a buffer salt solution wherein is positioned an electrode 36. Similarly the lower end of the tube 11' abuts tube 47 having apertures 51, covered by semipermeable membranes, contacting openings 52 and 53, an inner trough 56 and an outer trough 60, the openings 52, 53 and the troughs 56, 60 containing a buffer salt solution, the troughs being inter-connected via openings 57 covered by semi-permeable membranes 58, and the outer trough containing a counter-electrode 46. The tubes 27, 11, 11' and 47 are contained within the concentric tubes 40, 44, 64 which provide a cooling jacket, cooling fluid being passed therethrough between hose nipples 42 and 65, the cooling jacket spaces being inter-connected by passages 45 and 66. The density gradient fluid may be passed into the tube via the lower opening 67 or may be pipetted therein via the upper opening 43.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55290566A | 1966-05-25 | 1966-05-25 | |
| US14208471A | 1971-05-10 | 1971-05-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1169183A true GB1169183A (en) | 1969-10-29 |
Family
ID=26839745
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB44084/66A Expired GB1169183A (en) | 1966-05-25 | 1966-10-03 | Apparatus for Density Gradient Electrophoresis |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US3453200A (en) |
| DE (1) | DE1517927B2 (en) |
| FR (1) | FR1495824A (en) |
| GB (1) | GB1169183A (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL6813995A (en) * | 1968-09-30 | 1970-04-01 | ||
| US3664939A (en) * | 1970-04-22 | 1972-05-23 | Univ California | ISOELECTRIC FOCUSING AND FRACTIONATION AMPHOLYTES IN THERMALLY ENGENDERED pH GRADIENTS |
| US3847785A (en) * | 1972-02-14 | 1974-11-12 | Instr Specialties Co Inc | Electrophoresis aparatus |
| SE361829B (en) * | 1972-05-16 | 1973-11-19 | Lkb Produkter Ab | |
| SE390766B (en) * | 1972-12-19 | 1977-01-17 | Lkb Produkter Ab | PROCEDURE FOR RIVER ISOTACHOPHORES |
| US3980546A (en) * | 1975-06-27 | 1976-09-14 | Caccavo Francis A | Micropreparative electrophoresis apparatus |
| US5354440A (en) * | 1988-11-29 | 1994-10-11 | Isco, Inc. | Capillary electrophoresis technique |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1176098B (en) * | 1962-01-04 | 1964-08-20 | Lkb Produkter Ab | Device and method for electrophoretic separation |
| US3346479A (en) * | 1964-04-09 | 1967-10-10 | Scientific Industries | Preparative separation by a combination of gel separation and electrophoresis |
-
1966
- 1966-05-25 US US552905A patent/US3453200A/en not_active Expired - Lifetime
- 1966-10-03 GB GB44084/66A patent/GB1169183A/en not_active Expired
- 1966-10-04 FR FR78673A patent/FR1495824A/en not_active Expired
- 1966-10-19 DE DE19661517927 patent/DE1517927B2/en active Pending
-
1971
- 1971-05-10 US US27552D patent/USRE27552E/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DE1517927A1 (en) | 1970-05-21 |
| US3453200A (en) | 1969-07-01 |
| FR1495824A (en) | 1967-09-22 |
| DE1517927B2 (en) | 1971-07-01 |
| USRE27552E (en) | 1973-01-16 |
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