GB1090758A - Improvements in or relating to animal cell cultures - Google Patents

Improvements in or relating to animal cell cultures

Info

Publication number
GB1090758A
GB1090758A GB42946/63A GB4294663A GB1090758A GB 1090758 A GB1090758 A GB 1090758A GB 42946/63 A GB42946/63 A GB 42946/63A GB 4294663 A GB4294663 A GB 4294663A GB 1090758 A GB1090758 A GB 1090758A
Authority
GB
United Kingdom
Prior art keywords
medium
cells
gas
carbon dioxide
poisonous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB42946/63A
Inventor
Ronald Charles Telling
Clarence Jeffrey Stone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ANIMAL VIRUS RES INST
Wellcome Foundation Ltd
Original Assignee
ANIMAL VIRUS RES INST
Wellcome Foundation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ANIMAL VIRUS RES INST, Wellcome Foundation Ltd filed Critical ANIMAL VIRUS RES INST
Priority to GB42946/63A priority Critical patent/GB1090758A/en
Priority to IL22316A priority patent/IL22316A/en
Priority to ES0305348A priority patent/ES305348A1/en
Priority to BE654976A priority patent/BE654976A/xx
Priority to DEW37874A priority patent/DE1299798B/en
Priority to LU47250D priority patent/LU47250A1/xx
Priority to BR163884/64A priority patent/BR6463884D0/en
Priority to NL646412647A priority patent/NL142993B/en
Priority to FR993299A priority patent/FR1426975A/en
Publication of GB1090758A publication Critical patent/GB1090758A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

In the propagation of animal cells, in which the cells are suspended in an aqueous culture medium containing or being supplied with nutrients or additives known to be necessary or useful for the sustenance or multiplication of cells, the pH of the medium is kept between 7.3 and 7.8. For the control of the pH, the medium is contacted with a non-poisonous gas (e.g. by flowing the gas across the top of the medium) which contains carbon dioxide at a partial pressure higher than the equilibrium pressure corresponding to the carbon dioxide content of the medium should the pH of the medium rise above a desired or defined maximum pH, and a non-poisonous gas which contains carbon dioxide at a partial pressure below the above-mentioned equilibrium pressure is sparged through the medium should the pH of the medium fall below a desired or defined minimum pH. Alternatively, there is sparged through the medium a non-poisonous gas containing an adjusted amount of carbon dioxide for the regulation of the pH. The gas may consist of air, air diluted with an inert gas, or a mixture of non-poisonous gases preferably containing some oxygen. Non-poisonous anti-foam agents may be added to the medium to avoid frothing. Examples are directed to the propagation of cloned baby hamster kidney cells and to growing strains of foot-and-mouth disease viruses in the cells for the production of vaccine.ALSO:Strains of foot-and-mouth disease viruses are grown in cloned baby hamster kidney cells propagated in suspended culture in an aqueous culture medium regulated to a pH within the range 7.3 and 7.8. The virus particles are inactivated by a treatment with formaldehyde or acetyl-ethyliminine. Aluminium hydroxide is
GB42946/63A 1963-10-30 1963-10-30 Improvements in or relating to animal cell cultures Expired GB1090758A (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
GB42946/63A GB1090758A (en) 1963-10-30 1963-10-30 Improvements in or relating to animal cell cultures
IL22316A IL22316A (en) 1963-10-30 1964-10-22 Cell cultures
ES0305348A ES305348A1 (en) 1963-10-30 1964-10-27 A method for the propagation of animal cells. (Machine-translation by Google Translate, not legally binding)
BE654976A BE654976A (en) 1963-10-30 1964-10-28
DEW37874A DE1299798B (en) 1963-10-30 1964-10-29 Method for breeding animal cells in aqueous nutrient media
LU47250D LU47250A1 (en) 1963-10-30 1964-10-30
BR163884/64A BR6463884D0 (en) 1963-10-30 1964-10-30 PROCESS FOR PH CONTROL OF A CULTURE CONTAINING ANIMAL CELLS
NL646412647A NL142993B (en) 1963-10-30 1964-10-30 PROCEDURE FOR PREPARING A SUSPENSION OF ANIMAL CELLS, AND WITH THEREFORE A VIRUS VACCINE.
FR993299A FR1426975A (en) 1963-10-30 1964-10-30 Multiplication of animal cells in suspension cultures

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB42946/63A GB1090758A (en) 1963-10-30 1963-10-30 Improvements in or relating to animal cell cultures

Publications (1)

Publication Number Publication Date
GB1090758A true GB1090758A (en) 1967-11-15

Family

ID=10426658

Family Applications (1)

Application Number Title Priority Date Filing Date
GB42946/63A Expired GB1090758A (en) 1963-10-30 1963-10-30 Improvements in or relating to animal cell cultures

Country Status (9)

Country Link
BE (1) BE654976A (en)
BR (1) BR6463884D0 (en)
DE (1) DE1299798B (en)
ES (1) ES305348A1 (en)
FR (1) FR1426975A (en)
GB (1) GB1090758A (en)
IL (1) IL22316A (en)
LU (1) LU47250A1 (en)
NL (1) NL142993B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0387840A1 (en) * 1989-03-14 1990-09-19 Hitachi, Ltd. Method and apparatus for controlling cultivation conditions for animal cells

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4649117A (en) * 1985-03-15 1987-03-10 Hoffmann-La Roche Inc. Air lift bioreactor
US5081036A (en) * 1987-01-23 1992-01-14 Hoffmann-La Roche Inc. Method and apparatus for cell culture

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0387840A1 (en) * 1989-03-14 1990-09-19 Hitachi, Ltd. Method and apparatus for controlling cultivation conditions for animal cells
US5304483A (en) * 1989-03-14 1994-04-19 Hitachi, Ltd. Controlling cultivation conditions for animal cells

Also Published As

Publication number Publication date
ES305348A1 (en) 1965-03-16
LU47250A1 (en) 1965-04-30
NL6412647A (en) 1965-05-03
FR1426975A (en) 1966-02-04
BR6463884D0 (en) 1973-09-20
NL142993B (en) 1974-08-15
DE1299798B (en) 1969-07-24
BE654976A (en) 1965-04-28
IL22316A (en) 1968-09-26

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