GB1041814A - Therapeutic polypeptide from animal organs - Google Patents

Therapeutic polypeptide from animal organs

Info

Publication number
GB1041814A
GB1041814A GB4631261A GB4631261A GB1041814A GB 1041814 A GB1041814 A GB 1041814A GB 4631261 A GB4631261 A GB 4631261A GB 4631261 A GB4631261 A GB 4631261A GB 1041814 A GB1041814 A GB 1041814A
Authority
GB
United Kingdom
Prior art keywords
aqueous
aqueous solution
polypeptide
acid
therapeutic polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4631261A
Inventor
Per Jensen Laland
Soren Gustav Moe Laland
Jens Dedichen
Nils Thorsdalen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nyegaard and Co AS
Original Assignee
Nyegaard and Co AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to NL287198D priority Critical patent/NL287198A/xx
Application filed by Nyegaard and Co AS filed Critical Nyegaard and Co AS
Priority to GB4631261A priority patent/GB1041814A/en
Priority to SE13391/62A priority patent/SE323766B/xx
Priority to DK552462AA priority patent/DK108588C/en
Priority to CH1507862A priority patent/CH468191A/en
Priority to ES283674A priority patent/ES283674A1/en
Priority to BR14567962A priority patent/BR6245679D0/en
Priority to AT1008562A priority patent/AT253118B/en
Priority to DE19621492047 priority patent/DE1492047A1/en
Priority to FR919803A priority patent/FR2836M/en
Publication of GB1041814A publication Critical patent/GB1041814A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention relates to a therapeutic polypeptide material serving to induce human resistance to bacterial and viral infection and stress without exerting substantial pyrogenic or antigenic action, being substantially free from polypeptides of molecular weight less than 1000, being heat-stable at least up to 100 DEG C., being soluble in liquid phenol, in water and in aqueous bases, being precipitatable from aqueous solution by phosphotungstic acid, being soluble in aqueous sulphosalicylic acid, showing a green fluorescence and containing the following amino acids in polypeptide form, namely glutamic acid, aspartic acid, arginine, lysine, alamine, serine, valine, leucine, isoleucine, glycine, phenylalanine, histidine, proline, tyrosine and threonine, being substantially free from combined phosphorus, exhibiting the reactions typical of hexosamines and a positive anthrone and biuret reaction and having an antimitotic effect against Hela and Chang cells. The product is obtained by heating (e.g. to 100 DEG C.) an aqueous suspension or extract of an animal organ e.g. liver, kidney or spleen, and subjecting the product in aqueous solution to molecular fractionation to separate the required polypeptide from low molecular weight material. Various methods of fractionating are disclosed. In one example, fresh ox liver is treated with water containing phenol, heated to 100 DEG C. allowed to stand overnight, heated to 70 DEG C. and filtered, the filtrate concentrated, ethanol added, the precipitate removed and the ethanol extract concentrated to a paste. An aqueous solution of the paste is stirred with asbestos and the separated asbestos is eluted with (a) liquid phenol and (b) ammonia. These fractions are further treated on a Sephadex G50 column (cellulosic ion exchange resin). Pharmaceutical compositions comprise the above therapeutic polypeptide substance and a carrier e.g. capsules, tablets, dragees, suppositories, ampoules, syrups, linctuses, elixirs and emulsions.
GB4631261A 1961-12-27 1961-12-27 Therapeutic polypeptide from animal organs Expired GB1041814A (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
NL287198D NL287198A (en) 1961-12-27
GB4631261A GB1041814A (en) 1961-12-27 1961-12-27 Therapeutic polypeptide from animal organs
SE13391/62A SE323766B (en) 1961-12-27 1962-12-12
DK552462AA DK108588C (en) 1961-12-27 1962-12-19 A method of making a fluorescent polypeptide material.
CH1507862A CH468191A (en) 1961-12-27 1962-12-22 Process for the production of a polypeptide fraction
ES283674A ES283674A1 (en) 1961-12-27 1962-12-26 A method of producing a polypeptide material (Machine-translation by Google Translate, not legally binding)
BR14567962A BR6245679D0 (en) 1961-12-27 1962-12-26 PROCESS FOR THE PRODUCTION OF POLYPEPTIDIC MATERIAL
AT1008562A AT253118B (en) 1961-12-27 1962-12-27 Process for the production of an active substance of polypeptide nature with unspecific immunizing effect
DE19621492047 DE1492047A1 (en) 1961-12-27 1962-12-27 Process for the preparation of a polypeptide having an immunizing effect
FR919803A FR2836M (en) 1961-12-27 1962-12-27 Drug causing non-specific immunity.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB4631261A GB1041814A (en) 1961-12-27 1961-12-27 Therapeutic polypeptide from animal organs

Publications (1)

Publication Number Publication Date
GB1041814A true GB1041814A (en) 1966-09-07

Family

ID=10440733

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4631261A Expired GB1041814A (en) 1961-12-27 1961-12-27 Therapeutic polypeptide from animal organs

Country Status (1)

Country Link
GB (1) GB1041814A (en)

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