GB1017794A - Process for producing 5-ribonucleotides - Google Patents
Process for producing 5-ribonucleotidesInfo
- Publication number
- GB1017794A GB1017794A GB1201264A GB1201264A GB1017794A GB 1017794 A GB1017794 A GB 1017794A GB 1201264 A GB1201264 A GB 1201264A GB 1201264 A GB1201264 A GB 1201264A GB 1017794 A GB1017794 A GB 1017794A
- Authority
- GB
- United Kingdom
- Prior art keywords
- acid
- nucleotide
- sodium
- culture
- suitable amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
51-Inosinic acid, 51-xanthylic acid and 51-guanylic acid are obtained by contacting an enzyme source of bacterial origin, having nucleoside phosphotransferase activity for producing 51-nucleotide from the corresponding nucleoside and a phosphate donor, with a solution containing the corresponding nucleoside and a 51-nucleotide other than that to be synthesized, allowing biochemical phosphorylation to take place to produce the desired 51-nucleotide while maintaining the pH of the solution in the alkaline range, preferably between 7.0 and 10.0, the accumulated 51-ribonucleotide may be recovered by a conventional procedure. The production of the 51-nucleotide may be promoted by the addition of cupric and/or arsenic salts, the most suitable concentration of metallic salt being from 10- 3 to 10- 2 mole. Bacteria which have the ability to synthesize 51-nucleotide from the corresponding nucleoside and a phosphate donor include species which belong to the genera Pseudomonas, Serratia, Flavobacterium, Staphylococcus and Achromobacter. 51 - Nucleotides which may be used as the phosphate donor include 51 - adenylic acid, 51 - cytidylic acid, 51 - uridylic acid, 51-desoxyadenylic acid and 51-thymidylic acid. The enzyme source may be prepared by submerged culture, stationary culture or surface culture, preferably at a temperature of from 20 DEG to 37 DEG C. and for an incubation period of from 10 to 60 hours, in a culture medium containing a suitable amount of an organic or inorganic nitrogen source, such as peptone, meat extract, corn steep liquor, yeast extract, dry yeast, hydrolysate of soybean protein or an inorganic ammonium salt, and a suitable amount of carbon source, such as molasses, glucose or hydrolysate of starch, and a suitable amount of inorganic salts. The enzyme source may be in the form of, for example, living bacterial cells, cultured broth, living cell suspensions, dried cells, crude extract or enzyme preparation. Examples are given for the preparation of 51-inosinic acid in the presence of sodium-51-uridylate and copper sulphate or sodium arsenate, and of 51-guanylic acid in the presence of sodium-51-cytidylate or sodium-51-uridylate, and sodium arsenate.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1344663 | 1963-03-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1017794A true GB1017794A (en) | 1966-01-19 |
Family
ID=11833346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1201264A Expired GB1017794A (en) | 1963-03-22 | 1964-03-20 | Process for producing 5-ribonucleotides |
Country Status (4)
| Country | Link |
|---|---|
| CH (1) | CH415646A (en) |
| DE (1) | DE1445440A1 (en) |
| GB (1) | GB1017794A (en) |
| NL (1) | NL6402925A (en) |
-
1964
- 1964-03-16 DE DE19641445440 patent/DE1445440A1/en active Pending
- 1964-03-19 NL NL6402925A patent/NL6402925A/xx unknown
- 1964-03-20 GB GB1201264A patent/GB1017794A/en not_active Expired
- 1964-03-20 CH CH365964A patent/CH415646A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CH415646A (en) | 1966-06-30 |
| NL6402925A (en) | 1964-09-23 |
| DE1445440A1 (en) | 1969-02-13 |
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