GB1006259A - Fractionation of mixtures of agarose and agaropectin - Google Patents

Fractionation of mixtures of agarose and agaropectin

Info

Publication number
GB1006259A
GB1006259A GB2825764A GB2825764A GB1006259A GB 1006259 A GB1006259 A GB 1006259A GB 2825764 A GB2825764 A GB 2825764A GB 2825764 A GB2825764 A GB 2825764A GB 1006259 A GB1006259 A GB 1006259A
Authority
GB
United Kingdom
Prior art keywords
mixture
polyethylene glycol
aqueous liquid
agarose
dispersed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2825764A
Inventor
Alfred Polson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South African Inventions Development Corp
Original Assignee
South African Inventions Development Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by South African Inventions Development Corp filed Critical South African Inventions Development Corp
Priority to GB2825764A priority Critical patent/GB1006259A/en
Publication of GB1006259A publication Critical patent/GB1006259A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0036—Galactans; Derivatives thereof
    • C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

A process for fractionating mixtures of angarose and agaropectin comprises treating such mixture with an aqueous liquid containing dissolved or dispersed therein polyethylene glycol of molecular weight at least 300 in such concentrations as to render parts of the said mixture insoluble or indispersable in the aqueous liquid and separating the resulting insoluble or indispersable fraction from the aqueous liquid containing the balance of said mixture dissolved or dispersed therein. The mixture to be fractionated e.g. agar, may be dispersed or dissolved in an aqueous liquid, to which polyethylene glycol is then added, or a solution of dispersion of the mixture may be added to an aqueous solution of polyethylene glycol, or solutions of the mixture and of polyethylene glycol may be brought together concurrently, resulting in precipitation of an agarose fraction. Alternatively, the redissolution of an agarose fraction may be selectively suppressed by redissolving the mixture in presence of a sufficient concentration of polythylene glycol. The fractionation may be effected in several stages e.g. by a stagewise increase in the concentration of polyethylene glycol and removal of precipitated material between successive increases in concentration. Repeated alternate fractionation and redissolution or redispersion may be carried out. Polyethylene glycol may be removed from precipitated fractions by washing with a solvent, e.g. chloroform. Optimum conditions for precipitation, which depend on type of mixture to be fractionated, molecular weight of polyethylene glycol, temperature and ionic strength of the aqueous solutions, may be found by determination of a precipitation index (Specification 1,006,258). In an example, agarose is precipitated from a saline solution of agar at 50 DEG C. by addition of a 50% saline solution of polyethylene glycol of molecular weight 6000.
GB2825764A 1962-12-19 1962-12-19 Fractionation of mixtures of agarose and agaropectin Expired GB1006259A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2825764A GB1006259A (en) 1962-12-19 1962-12-19 Fractionation of mixtures of agarose and agaropectin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2825764A GB1006259A (en) 1962-12-19 1962-12-19 Fractionation of mixtures of agarose and agaropectin

Publications (1)

Publication Number Publication Date
GB1006259A true GB1006259A (en) 1965-09-29

Family

ID=10272789

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2825764A Expired GB1006259A (en) 1962-12-19 1962-12-19 Fractionation of mixtures of agarose and agaropectin

Country Status (1)

Country Link
GB (1) GB1006259A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2210444A1 (en) * 1972-12-15 1974-07-12 Anvar Rehydratable dry gels prepn. - by treating aqs. gel with polyalcohol aqs. soln and and dehydrating
ES2166296A1 (en) * 1999-12-17 2002-04-01 Univ Alcala Henares Extraction of nucleic acids soaked in agarose gel consists of preparation of macromolecules, with thermal fusion of gel and gelling with phase separation, for uses in pharmaceuticals
WO2010109289A1 (en) * 2009-03-24 2010-09-30 Council Of Scientific & Industrial Research Process for the preparation of agarose polymer from seaweed extractive

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2210444A1 (en) * 1972-12-15 1974-07-12 Anvar Rehydratable dry gels prepn. - by treating aqs. gel with polyalcohol aqs. soln and and dehydrating
ES2166296A1 (en) * 1999-12-17 2002-04-01 Univ Alcala Henares Extraction of nucleic acids soaked in agarose gel consists of preparation of macromolecules, with thermal fusion of gel and gelling with phase separation, for uses in pharmaceuticals
WO2010109289A1 (en) * 2009-03-24 2010-09-30 Council Of Scientific & Industrial Research Process for the preparation of agarose polymer from seaweed extractive
CN102439047A (en) * 2009-03-24 2012-05-02 科学与工业研究会 Process for the preparation of agarose polymer from seaweed extractive
JP2012521475A (en) * 2009-03-24 2012-09-13 カウンシル・オヴ・サイエンティフィック・アンド・インダストリアル・リサーチ Method for preparing agarose polymer from seaweed extract
CN102439047B (en) * 2009-03-24 2014-05-07 科学与工业研究会 Process for the preparation of agarose polymer from seaweed extractive

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