FR3112270A1 - MASK HAVING ANTIBACTERIAL AND ANTIVIRAL FUNCTIONS AND METHOD FOR MAKING IT - Google Patents

Abstract

The present application provides a mask having antibacterial and antiviral functions and a method of manufacturing the same. The mask comprises a mask body, where the mask body comprises an outer cover layer, an inner cover layer and at least two straps, the inner cover layer is made of the following materials: cotton, hemp and antibacterial fibers, where , a strength of the mask body is ≥300 CN, an elongation at break of the mask body is ≥6%, a twist of the twist yarn of the mask body is 10 twists/inch, a twist direction of the twist yarn of the mask body is S-twist. Compared with the prior art, by adopting the structure, the mask of the present application has novel coronavirus inactivation function and antiviral effect, and is convenient to use; the mask also has a surgical mask function; in addition to the prevention of synthetic blood, the mask has an additional function compared with ordinary medical mask, that is, the particle filtration efficiency is more than 50%; and what's more, the mask can eliminate peculiar smell, and can be washable and reusable for 10 days. Abstract Figure: Fig. 1

Description

MASK HAVING ANTIBACTERIAL AND ANTIVIRAL FUNCTIONS AND METHOD FOR MAKING IT

CROSS-REFERENCE TO RELATED REQUESTS

The application claims priority from Chinese Patent Application No. 202010663611.X, filed July 10, 2020.

The present application relates to a mask having antibacterial and antiviral functions and a method of manufacturing the same.

BACKGROUND

Currently, a mask existing in the prior art, including a mask body, does not have antibacterial and antiviral functions, so it is inconvenient to use.

Accordingly, the present application provides a mask having antibacterial and antiviral functions and a method of manufacturing the same.

SUMMARY

In view of the aforementioned defect in the prior art, the present application aims to provide a mask having antibacterial and antiviral functions and a manufacturing method thereof, wherein the mask has an antiviral effect and is convenient to use.

To this end, the present application provides a mask having antibacterial and antiviral functions, comprising a mask body, characterized in that the mask body comprises an outer covering layer, an inner covering layer and at least two straps, the inner lining layer is made of the following materials: cotton, hemp and antibacterial fibers, where,

a mask body strength is ≥300 CN, a mask body breaking elongation is ≥6%;

one twist of twist of mask body yarn is 10 twists/inch, one twist direction of twist of mask body yarn is S-twist.

This application also provides a method for manufacturing the mask having antibacterial and antiviral functions, characterized in that it comprises the following steps:

step 1, preparing a sample mask body: cut an outer covering layer of a mask body into a 1*1 cm sheet, wash the mask body twice using deionized water, dry the mask body to air, and sterilizing the mask body in pressurized steam equipment for further use;

stage 2, contamination:

(1) in a biosafety cabinet, divide the sterilized materials into three types, each with eight pieces, place each type of the sterilized materials into a twelve-hole cell culture plate;

(2) Aspirate 50 µl of virus suspension (106TCID50/ml) using a pipette and drop the virus suspension onto the surfaces of the sterilized materials, and place the sterilized materials at room temperature (25-27°C) and a relative humidity of 35%; and

(3) elute viruses 0 h, 1 h, 2 h, 6 h, 24 h, 48 h and 72 h after addition of the viral suspension;

step 3, viral titration, pre-add Vero cells to a ninety-six-hole cell culture plate, perform 10-fold dilution continuously on viral eluate, add viral eluate to cell culture plate of ninety-six holes for viral titration, observe and record a cytopathic effect (CPE) on the fourth day after titration, and calculate a TCID50 using the Reed-Muench method.

Further, the process for manufacturing the mask having antibacterial and antiviral functions where in the viral elution step of step 2, 500 µl of a cell maintenance medium is added into the cell culture plate of twelve holes, then a surface of the cell maintenance medium is repeatedly blown and beaten, the eluate is collected, and three repetitions of the holes are made for each sample at each time.

Also, the manufacturing process of the mask having antibacterial and antiviral functions where, the number of cells per unit of Vero cells is 2*104 cells/hole.

As mentioned above, the mask having antibacterial and antiviral functions and the manufacturing method thereof provided by the present application have the following beneficial effects: compared to the prior art, by adopting the structure and method of manufacture of the present application, the mask of the present application has novel coronavirus inactivation function and antiviral effect, and is convenient to use.

A more detailed description will be provided in this application in conjunction with specific embodiments below.

The is a schematic structural diagram of a mask having antibacterial and antiviral functions of the present application.

DETAILED DESCRIPTION OF EMBODIMENTS

Modes of implementation of this application will be described through specific embodiments below. Other advantages and effects of the present application can easily be understood by those skilled in the art from the disclosed contents of the description.

It should be noted that the structure, ratio, size, etc. shown in the accompanying drawings of the description are simply used to correspond to the disclosed content of the description for the understanding and reading of those skilled in the art, and are not used to limit the conditions for implementing this application, so they have no technically substantial meaning. At the same time, terms such as "upper", "lower", "left", "right", "middle", "a/an" used in the description are merely for convenience of description, and are not used to limit the scope of implementation of this application, and the change and adjustment of their relative relationship shall be considered as the scope of implementation of the predefined application as long as they do not substantially change the technical content. The specific structure can be described with reference to the accompanying drawings of the patent application.

Please refer to the , the present application provides a mask having antibacterial and antiviral functions, comprising a mask body 1, wherein the mask body 1 comprises an outer covering layer 10, an inner covering layer 11 and at least two straps 12, the layer of internal lining 11 is made of the following materials: cotton, hemp and antibacterial fibers, where:

a strength of the mask body 1 is ≥300 CN, and an elongation at break of the mask body 1 is ≥6%;

one twist of twist yarn of mask body 1 is 10 twists/inch, one twist direction of twist yarn of mask body 1 is S twist.

Note that, compared with the prior art, by adopting the structure, the mask of the present application has novel coronavirus inactivation function and antiviral effect, and is convenient to use. It should be noted that, the mask also has a surgical mask function; in addition to preventing synthetic blood from penetrating, the mask has an additional function compared to ordinary medical mask, that is, the particle filtration efficiency is more than 50%; moreover, the mask can eliminate peculiar smell, and can be washable and reusable for 10 days.

The present application further provides a mask having antibacterial and antiviral functions and a method of manufacturing the same, wherein the method comprises the following steps:

step 1, preparing a mask body sample: an outer coating layer of a mask body is cut into a 1*1 cm sheet, the mask body is washed twice using deionized water , the mask body is air-dried and sterilized in pressurized steam equipment for later use;

stage 2, contamination:

(1) In a biosafety cabinet, the sterilized materials are divided into three types, each with eight pieces, and each type of the sterilized materials is placed in a twelve-hole cell culture plate;

(2) 50 µl of virus suspension (106TCID50/ml) is aspirated using a pipette and added dropwise to the surfaces of the sterilized materials, and the sterilized materials are placed at room temperature (25-27°C) and humidity. 35% relative; and

(3) viruses are eluted 0 h, 1 h, 2 h, 6 h, 24 h, 48 h and 72 h after addition of the viral suspension;

step 3, viral titration, Vero cells are pre-added into a ninety-six-hole cell culture plate, viral eluate is continuously diluted 10-fold and added into the ninety-hole cell culture plate sixteen holes for viral titration, a cytopathic effect (CPE) is observed and recorded on the fourth day after titration, and a TCID50 is calculated using the Reed-Muench method. Note that the mask of the present application has novel coronavirus inactivation function and antiviral effect, and is convenient to use by adopting the manufacturing method of the present application.

The first test report of the mask having antibacterial and antiviral functions manufactured by adopting the aforementioned method of the present application is as follows: Report No.: WT204043882 3 of 4 testing item Method standard standard requirement Result Individual conclusion 1. Nose clip
(refer to YY/T 0969-2013)
Section 4.3.1
Section 4.3.2 YY/T 0969-2013 A mask must be equipped with a nose clip and the nose clip is made of a malleable material.
The length of the nose clip should not be less than 8.0 cm. 1#~3# Compliance
Length (cm)
1# 2# 3#
10.5 10.5 10.7 Compliance 2. Mask strap
(refer to YY/T 0969-2013)
Section 4.4.1
Section 4.4.2 YY/T 0969-2013 A mask should be convenient to wear and remove
The breaking strength of a connection point between each strap and a mask body must not be less than 10 N Compliance
1#~3#: No slippage of the mask strap from the mask body connection point was found
(fixed load: 10 N, duration 5 s) Compliance 3. Airflow resistance (Pa/cm ² )
(refer to YY/T 0969-2013) YY/T 0969-2013 ≤49 1# 2# 3#
48.9 47.5 48.0
(Flow: 8 L/min) Compliance 4. Bacterial filtration efficiency (%)
(refer to YY/T 0969-2013) YY 0469-2011 ≥95 1# 2# 3#
100 100 100 Compliance

The second test report of the mask having antibacterial and antiviral functions manufactured by adopting the aforementioned method of the present application is as follows: Report No.: WT204043884 3 of 3 testing item Method standard standard requirement Result Individual conclusion 1. Antibacterial rate
(refer to FZ/T73023-2006)
Staphylococcus aureus
Escherichia coli
Candida albicans Appendix D of FZ/T73023-2006
AAA grade
≥80
≥70
≥60
86
76
66 Compliance

Notes: The samples are washed 4 times according to the simplified washing conditions and procedures in C.4 of Appendix C of FZ/T73023-2006, which is equivalent to the samples being washed 20 times with water.

The third test report of the mask having antibacterial and antiviral functions manufactured by adopting the aforementioned method of the present application is as follows: Report No.: WT204053932 3 of 4 testing item Method standard standard requirement Result Individual conclusion 1. Bacterial filtration efficiency (%) (YY/T 0469-2011) YY 0469-2011 ≥95 1# 2# 3#
100 100 99 Compliance 2. Filtration efficiency
Particle filtration efficiency (%) (YY 0469-2011) YY 0469-2011 ≥30 1# 2# 3#
92.47 95.25 92.08
Aerosol particle: NaCl
Aerosol concentration: 20 mg / m ³
Temperature: 24.0℃ Relative humidity: 34.5% (airflow: 30 L/min) Compliance

In this embodiment, in the virus elution step of step 2, 500 µl of a cell maintenance medium is added to the twelve-hole cell culture plate, then a surface of the cell maintenance medium is added. is repeatedly blown and beaten, the eluate is collected, three repetitions of holes are made for each sample at each time.

In this embodiment, the number of cells per unit of Vero cells is 2*104 cells/hole. As mentioned above, the mask having antibacterial and antiviral functions and the manufacturing method thereof provided by the present application have the following beneficial effects: compared to the prior art, by adopting the structure and method of manufacture of the present application, the mask of the present application has novel coronavirus inactivation function and antiviral effect, and is convenient to use.

In summary, the present application overcomes the shortcoming of the prior art, and has high industrial use value.

The aforementioned embodiments merely explain by way of example the principle and effect of the present application, but are not used to limit the present application.

Claims (4)

Mask having antibacterial and antiviral functions, comprising a mask body, characterized in that the mask body comprises an outer covering layer, an inner covering layer and at least two straps, the inner covering layer is made of the following materials : cotton, hemp and antibacterial fibers, where,
a mask body strength is ≥300 CN, a mask body breaking elongation is ≥6%;
one twist of the mask body twist is 10 twists/inch, one twist direction of the mask body twist is S twist. Process for manufacturing the mask having antibacterial and antiviral functions according to claim 1, characterized in that it comprises the following steps:
step 1, preparation of a sample mask body: cut an outer coating layer of a mask body into a 1*1 cm sheet, wash the mask body twice using deionized water, dry the mask body to air, and sterilizing the mask body in pressurized steam equipment for further use;
step 2, contamination: (1) in a biosafety cabinet, divide the sterilized materials into three types, each with eight pieces, place each type of the sterilized materials into a twelve-hole cell culture plate;
(2) Aspirate 50 µl of virus suspension (106TCID50/ml) using a pipette and drop the virus suspension onto the surfaces of the sterilized materials, and place the sterilized materials at room temperature (25-27°C) and a relative humidity of 35%; and
(3) elute viruses 0 h, 1 h, 2 h, 6 h, 24 h, 48 h and 72 h after addition of the viral suspension;
step 3, viral titration, pre-add Vero cells to a ninety-six-hole cell culture plate, perform 10-fold dilution continuously on viral eluate, add viral eluate to cell culture plate of ninety-six holes for viral titration, observe and record a cytopathic effect (CPE) on the fourth day after titration, and calculate a TCID50 using the Reed-Muench method. Process for manufacturing the mask having antibacterial and antiviral functions according to claim 2, characterized in that in the viral elution step of step 2, 500 µl of a cell maintenance medium are added to the culture plate cell from twelve holes, then a surface of the cell maintenance medium is repeatedly blown and beaten, the eluate is collected, and three hole repetitions are made for each sample at each time point. A method of manufacturing the mask having antibacterial and antiviral functions according to claim 2, characterized in that the number of cells per unit of Vero cells is 2*104 cells/hole.