FR3099770B1 - METHOD OF ANALYSIS OF THE LOSS OF BACTERIAL DIVERSITY OF THE HUMAN INTESTINAL MICROBIOME - Google Patents

METHOD OF ANALYSIS OF THE LOSS OF BACTERIAL DIVERSITY OF THE HUMAN INTESTINAL MICROBIOME Download PDF

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Publication number
FR3099770B1
FR3099770B1 FR1908970A FR1908970A FR3099770B1 FR 3099770 B1 FR3099770 B1 FR 3099770B1 FR 1908970 A FR1908970 A FR 1908970A FR 1908970 A FR1908970 A FR 1908970A FR 3099770 B1 FR3099770 B1 FR 3099770B1
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loss
bacterial diversity
human intestinal
diversity
analysis
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FR1908970A
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French (fr)
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FR3099770A1 (en
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Alessandra Cervino
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Luxia Scient
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Luxia Scient
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention décrit une méthode permettant de diagnostiquer la perte de diversité bactérienne du microbiote intestinal humain, in vitro, à partir d’un échantillon de selle. Cette méthode implique un ensemble d’étapes calibrées permettant une reproductibilité parfaite des résultats, et une fiabilité accrue du diagnostic. Les inventeurs ont ainsi identifié une valeur de référence permettant de séparer les individus en deux groupes en fonction de leur niveau de diversité bactérienne. Les personnes diagnostiquées pour avoir une faible diversité sont statistiquement plus fréquemment en mauvaise santé que les autres. Des méthodes de traitement appropriées peuvent donc être développées spécifiquement pour ces individus.The present invention describes a method for diagnosing the loss of bacterial diversity of the human intestinal microbiota, in vitro, from a stool sample. This method involves a set of calibrated steps allowing perfect reproducibility of results and increased diagnostic reliability. The inventors have thus identified a reference value making it possible to separate the individuals into two groups according to their level of bacterial diversity. People diagnosed with low diversity are statistically more frequently in poor health than others. Appropriate treatment methods can therefore be developed specifically for these individuals.

FR1908970A 2019-08-05 2019-08-05 METHOD OF ANALYSIS OF THE LOSS OF BACTERIAL DIVERSITY OF THE HUMAN INTESTINAL MICROBIOME Active FR3099770B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR1908970A FR3099770B1 (en) 2019-08-05 2019-08-05 METHOD OF ANALYSIS OF THE LOSS OF BACTERIAL DIVERSITY OF THE HUMAN INTESTINAL MICROBIOME

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1908970 2019-08-05
FR1908970A FR3099770B1 (en) 2019-08-05 2019-08-05 METHOD OF ANALYSIS OF THE LOSS OF BACTERIAL DIVERSITY OF THE HUMAN INTESTINAL MICROBIOME

Publications (2)

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FR3099770A1 FR3099770A1 (en) 2021-02-12
FR3099770B1 true FR3099770B1 (en) 2023-01-13

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FR1908970A Active FR3099770B1 (en) 2019-08-05 2019-08-05 METHOD OF ANALYSIS OF THE LOSS OF BACTERIAL DIVERSITY OF THE HUMAN INTESTINAL MICROBIOME

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112992351B (en) * 2021-03-09 2024-03-08 广西爱生生命科技有限公司 Feature expression method and evaluation method for human intestinal health state

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2909336T3 (en) * 2012-10-17 2018-08-20 Agronomique Inst Nat Rech DETERMINATION OF REDUCED GAS BACTERIA DIVERSITY
FR3011009B1 (en) * 2013-09-25 2016-11-25 Oreal BACTERIAL SIGNATURE OF ATOPIC DERMATITIS AND USE THEREOF IN THE PREVENTION AND / OR TREATMENT OF THIS PATHOLOGY
PL235777B1 (en) * 2015-07-10 2020-10-19 Univ Jagiellonski Starters, method for microbiological analysis of biomaterial, application of the NGS sequencing method in microbiological diagnostics and the diagnostic set
RU2724590C2 (en) * 2015-08-04 2020-06-25 Сосьете Де Продюи Нестле С.А. Nutrient compositions with 2fl and lnnt for use in inducing intestinal microbiota, which is similar to intestinal microbiota of infants in breastfeeding

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