FR3072097B1 - Procede de detection de molecules d'acides nucleiques par hyperthermie magnetique et ensemble permettant une telle detection - Google Patents

Procede de detection de molecules d'acides nucleiques par hyperthermie magnetique et ensemble permettant une telle detection Download PDF

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Publication number
FR3072097B1
FR3072097B1 FR1759346A FR1759346A FR3072097B1 FR 3072097 B1 FR3072097 B1 FR 3072097B1 FR 1759346 A FR1759346 A FR 1759346A FR 1759346 A FR1759346 A FR 1759346A FR 3072097 B1 FR3072097 B1 FR 3072097B1
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Prior art keywords
detection
nucleic acid
stranded
acid molecules
molecule
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FR1759346A
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FR3072097A1 (fr
Inventor
Marie-Charlotte Horny
Jean-Michel Siaugue
Vincent Dupuis
Mathieu Lazerges
Jean Gamby
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Centre National de la Recherche Scientifique CNRS
Universite Pierre et Marie Curie Paris 6
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite Pierre et Marie Curie Paris 6
Institut National de la Sante et de la Recherche Medicale INSERM
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Priority to FR1759346A priority Critical patent/FR3072097B1/fr
Priority to US16/753,404 priority patent/US11713481B2/en
Priority to PCT/EP2018/077080 priority patent/WO2019068844A1/fr
Priority to EP18779397.1A priority patent/EP3692162A1/fr
Publication of FR3072097A1 publication Critical patent/FR3072097A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/305—Denaturation or renaturation by physical action
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/116—Nucleic acid detection characterized by the use of physical, structural and functional properties electrical properties of nucleic acids, e.g. impedance, conductivity or resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/155—Particles of a defined size, e.g. nanoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé de pré-concentration et de détection d'au moins une molécule monocaténaire d'acides nucléiques cible, le procédé comprenant des étapes de génération d'un écoulement de liquide comprenant au moins une nanoparticule magnétique dans un micro-canal et une pluralité de molécules monocaténaires d'acides nucléiques sondes attachées à la nanoparticule, de génération d'un champ magnétique alternatif dans la partie du micro-canal au moyen de l'électroaimant, le champ magnétique présentant une intensité et une fréquence adaptées pour entraîner une hyperthermie magnétique des nanoparticules de sorte à provoquer une dénaturation du duplex formé par la molécule monocaténaire d'acides nucléiques sonde et par la molécule monocaténaire d'acides nucléiques cible, et une détection de la molécule monocaténaire cible dispersée dans le liquide.
FR1759346A 2017-10-05 2017-10-05 Procede de detection de molecules d'acides nucleiques par hyperthermie magnetique et ensemble permettant une telle detection Active FR3072097B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1759346A FR3072097B1 (fr) 2017-10-05 2017-10-05 Procede de detection de molecules d'acides nucleiques par hyperthermie magnetique et ensemble permettant une telle detection
US16/753,404 US11713481B2 (en) 2017-10-05 2018-10-05 Process for detecting nucleic acid molecules by magnetic hyperthermia and assembly enabling such detection
PCT/EP2018/077080 WO2019068844A1 (fr) 2017-10-05 2018-10-05 Procede de detection de molecules d'acides nucleiques par hyperthermie magnetique et ensemble permettant une telle detection
EP18779397.1A EP3692162A1 (fr) 2017-10-05 2018-10-05 Procede de detection de molecules d'acides nucleiques par hyperthermie magnetique et ensemble permettant une telle detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1759346A FR3072097B1 (fr) 2017-10-05 2017-10-05 Procede de detection de molecules d'acides nucleiques par hyperthermie magnetique et ensemble permettant une telle detection

Publications (2)

Publication Number Publication Date
FR3072097A1 FR3072097A1 (fr) 2019-04-12
FR3072097B1 true FR3072097B1 (fr) 2021-10-22

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FR1759346A Active FR3072097B1 (fr) 2017-10-05 2017-10-05 Procede de detection de molecules d'acides nucleiques par hyperthermie magnetique et ensemble permettant une telle detection

Country Status (4)

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US (1) US11713481B2 (fr)
EP (1) EP3692162A1 (fr)
FR (1) FR3072097B1 (fr)
WO (1) WO2019068844A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112949141B (zh) * 2021-03-26 2022-11-04 福州大学 基于有限元方法的磁纳米颗粒浓度分布预测方法
FR3129677B1 (fr) * 2021-11-30 2023-10-20 Univ Paris Saclay Fixation d’acide nucléique sur une électrode de platine

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7906345B2 (en) * 2003-11-12 2011-03-15 The Board Of Trustees Of The Leland Stanford Junior University Magnetic nanoparticles, magnetic detector arrays, and methods for their use in detecting biological molecules
WO2007089564A2 (fr) * 2006-01-26 2007-08-09 The Regents Of The University Of California dosage immunomagnétique de microcanal
EP2110175A1 (fr) * 2008-03-20 2009-10-21 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Procédé et dispositif de commande thermique de réactions biologiques et chimiques sous l'influence de particules ou de billes magnétiques et de champs magnétiques variables
US9030215B2 (en) * 2011-10-04 2015-05-12 The Regents Of The University Of California Corporation Real-time, label-free detection of nucleic acid amplification in droplets using impedance spectroscopy and solid-phase substrate

Also Published As

Publication number Publication date
FR3072097A1 (fr) 2019-04-12
EP3692162A1 (fr) 2020-08-12
US11713481B2 (en) 2023-08-01
US20200263241A1 (en) 2020-08-20
WO2019068844A1 (fr) 2019-04-11

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