FR3059102A1 - PLASMA TAKING DEVICE AND SAMPLING METHOD - Google Patents

Abstract

The invention relates to a device (10) for sampling a sample of cored frozen plasma comprising a rotary motor (14), a hollow wick (18) and a hollow wick receiving element, the rotary motor (14). being adapted to rotate the hollow wick (18) and the hollow wick (18) being fluidly isolated from the remainder of the sampling device (10) by the receiving member. The invention also relates to a method for withdrawing frozen plasma by means of the sampling device (10).

Description

® FRENCH REPUBLIC

NATIONAL INSTITUTE OF INDUSTRIAL PROPERTY © Publication number:

(to be used only for reproduction orders)

©) National registration number

059 102

61297

COURBEVOIE © IntCI ⁸ : G 01 N 1/08 (2017.01)

PATENT INVENTION APPLICATION

A1

©) Date of filing: 21.11.16. © Applicant (s): FRENCH ESTABLISHMENT OF (© Priority: BLOOD Public establishment - FR. @ Inventor (s): BLOT SANDRINE and JORGE FER- REIRA ANTHONY. (43) Date of public availability of the request: 25.05.18 Bulletin 18/21. ©) List of documents cited in the report preliminary research: Refer to end of present booklet (© References to other national documents ® Holder (s): FRENCH ESTABLISHMENT OF related: BLOOD Public establishment. ©) Extension request (s): © Agent (s): CABINET BEAU DE LOMENIE.

? 4) PLASMA COLLECTION DEVICE AND COLLECTION METHOD.

FR 3 059 102 - A1 _ The invention relates to a device (10) for sampling a plasma sample frozen by coring comprising a rotary motor (14), a hollow wick (18) and a receiving element for the hollow wick , the rotary motor (14) being intended to rotate the hollow wick (18) and the hollow wick (18) being fluidly isolated from the rest of the sampling device (10) by the receiving element.

The invention also relates to a method for collecting frozen plasma using the sampling device (10).

BACKGROUND OF THE INVENTION The present disclosure relates to a device for taking a plasma sample.

Generally, after a plasma donation, the plasma collected in a pocket and is frozen to be stored.

However, after freezing the plasma, it may be necessary to take plasma samples.

The plasma bag is then completely thawed in order to ensure good homogeneity of the sample taken, a sample of liquid plasma is taken and the plasma bag is again frozen.

This defrosting process is relatively slow and, in order to guarantee the quality of the plasma contained in the bag, a given plasma bag can only undergo a limited number of defrosting / freezing cycles.

OBJECT AND SUMMARY OF THE INVENTION The present description aims to remedy these drawbacks at least in part.

To this end, the present disclosure relates to a device for taking a sample of frozen plasma by coring comprising a rotary motor, a hollow wick and a receiving element of the hollow wick, the rotary motor being intended to rotate the hollow wick and the hollow wick being fluidly isolated from the rest of the sampling device by the receiving element.

The hollow wick can be rotated by the rotary motor of the sampling device, it is possible to take a sample by coring and therefore to take a sample of frozen plasma. It is therefore no longer necessary to thaw the plasma bag before sampling.

Furthermore, the hollow wick being received in a receiving element of the hollow wick which fluidly isolates the hollow wick from the rest of the device, the sample taken is in contact with the hollow wick and the receiving element. The sample collected, in frozen form, is not in contact with other parts of the collection device.

The rotary motor being intended to rotate the hollow wick, it is understood that the rotary motor is able to rotate the receiving element of the hollow wick relative to the rest of the device and therefore also the hollow wick which is mounted on the device in the receiving element.

Before the first coring, the hollow wick and the receiving element can be sterile.

The sampling device can be portable.

The portable sampling device is compact and can be easily manipulated.

The hollow wick can be held in the receiving element by radial mechanical tightening.

Thus, the hollow wick, of tubular shape, can have a diameter substantially equal to the diameter of the receiving element, it is understood that the hollow wick has a diameter slightly less than the diameter of the receiving element in order to be able be received in the receiving element.

The receiving element can be held in the sampling device by radial mechanical tightening.

Radial tightening is simple to set up and allows the rotation to be transmitted to the hollow drill bit in a simple and effective manner.

The receiving element can be a cap, the hollow wick can be partially received in the cap and can include a free end not received in the cap.

The cap may include a bottom and a tubular part extending axially from the bottom. This shape is substantially complementary to the shape of the hollow wick.

The cap can be sterilized and reused.

The cap can be made of plastic.

Thus, after use, the cap can be discarded. This prevents contamination of the collection device by the plasma samples collected. Indeed, the cap surrounds the hollow wick and isolates the hollow wick from the rest of the sampling device.

The cap can be made of flexible plastic.

Thus, the plastic can adapt to the tightening and the pressure exerted by the sampling device.

The free end of the hollow wick can be bevelled or in the shape of a "V".

This allows easier coring than if the free end of the hollow drill bit is not bevelled.

The hollow wick can be made of stainless steel.

This material is easy to clean and sterilize. Furthermore, the stainless steel wick is advantageously degreased in order to avoid contaminating the sample taken and the plasma contained in the frozen plasma bag with fat.

The sampling device may comprise a drill driver comprising a mandrel, the receiving element being mounted in the mandrel.

The drill driver is easy to handle and compact.

The present disclosure also relates to a method for collecting a sample of frozen plasma by means of a sampling device, the method comprising the following steps:

a) maintaining a pocket of frozen plasma by a pocket support;

b) positioning of a receiving element in the sampling device;

c) positioning of a hollow wick in the receiving element, the receiving element fluidly isolating the hollow wick from the rest of the sampling device;

d) positioning the hollow wick in an orifice of the frozen plasma bag;

e) rotating the hollow wick by a rotary motor of the device for sampling and coring the frozen plasma by the hollow wick in order to take a sample of frozen plasma;

f) removal of the frozen wick from the frozen plasma; and

g) extraction of the frozen plasma sample from the hollow wick. The frozen plasma bag is held in a support.

The operator therefore has both hands free. The hollow wick being positioned in the orifice of the plasma bag, the core is cored and the frozen plasma is taken through this orifice.

It is understood that this orifice allows access to the sample. This orifice can be placed in a tube or in a chimney.

As the core drilling is done by rotation of the hollow drill bit, the accuracy of the core drilling is more important than the taking of the sample was carried out by driving in a hollow drill bit without rotation of the hollow drill bit.

The extraction of the frozen plasma sample from the hollow wick can be achieved by separating the hollow wick from the receiving element and by inserting a stainless steel rod in the hollow wick which is itself arranged in a tube, for example plastic, to collect the frozen plasma sample contained in the hollow wick. Extraction can also be carried out by placing the hollow wick and the frozen plasma sample in a container and allowing the frozen plasma sample to melt at least partially.

Steps d) to g) can be repeated on the frozen plasma bag without changing the hollow wick and receiving element.

It is thus possible to take several samples of frozen plasma in the same pocket of frozen plasma without having to change the hollow wick and / or the receiving element. Thus, depending on the volume of plasma to be sampled, the coring operation can be repeated several times with the same hollow wick and the same receiving element. The volume of sample that can be taken with the same hollow wick and the same receiving element can be easily adapted. It suffices to carry out a greater or lesser number of cores. It is not necessary to change the hollow drill bit and / or the receiving element with each new coring.

It is therefore understood that the sampling device is devoid of means which make the use of the hollow wick impossible after a single use.

When a volume of frozen plasma withdrawn is at least equal to a predetermined volume, the orifice of the frozen plasma bag can be sealed.

Passing through the orifice of the plasma pocket, the plasma pocket is not damaged. This orifice can be hermetically closed, for example by welding a tube opening out from this orifice. The orifice can also lead into a "chimney", which is a tube protected by a plastic envelope. This chimney can be closed using a waterproof perforator or a plug adapted to the size of the chimney. The frozen plasma bag can be returned to a freezer suitable for future use.

The frozen plasma bag can then be placed in a sealed bag which is hermetically sealed around the frozen plasma bag. The assembly is then returned to a freezer suitable for later use. This makes it possible to reduce, or even avoid, any risk of leakage during the subsequent thawing of the frozen plasma bag.

The frozen plasma bag can be a first frozen plasma bag and, before taking a frozen plasma sample from a second frozen plasma bag, different from the first frozen plasma bag, the hollow wick can be changed and the receiving element.

Thus, a new hollow wick and a new receiving element are used when it is desired to take a plasma sample from a new plasma bag. The risks of contamination of a plasma pocket with the content of another plasma pocket are reduced, that is to say of inter-sample contamination.

Brief Description of the Drawings Other characteristics and advantages of the invention will emerge from the following description of embodiments of the invention, given by way of nonlimiting examples, with reference to the appended figures, in which:

- Figure 1 is a schematic perspective view of a sampling device;

- Figure 2 is a schematic view of the coring step;

- Figure 3 is a schematic view of a frozen plasma bag;

- Figure 4 is a flowchart showing the steps of a sampling process.

Detailed description of the invention [0045] FIG. 1 represents a device 10 for taking a sample of frozen plasma by coring. The sampling device 10 of FIG. 1 comprises a drill-driver 12, for example a cordless drill-driver, comprising a rotary motor 14 and a mandrel 16. The mandrel 16 receives an element for receiving a hollow bit 18, in the example of FIG. 1, the receiving element is a cap 20.

The hollow wick 18 is generally tubular and has a first end 18A received in the cap 20 and a free end 18B not received in the cap 20. The axis of rotation of the hollow wick 18 on itself defines an axial direction A.

The cap 20 may for example be a plastic cap of pocket perforator. In FIG. 1, the cap 20 comprises a bottom 20A and a tubular part 20B extending axially from the bottom.

The first end 18A of the hollow wick 18 can be in abutment against the bottom 20A of the cap 20.

The cap 20 therefore fluidly isolates the hollow wick 18 from the rest of the sampling device 10. It is understood that the frozen plasma sample which may be contained in the hollow wick 18 after coring is in contact with the hollow wick 18 and the cap 20 but is not in contact with the rest of the sampling device 10.

The hollow wick 18 and the cap 20 are the only parts of the sampling device 10 which are in contact with the frozen plasma.

The hollow wick 18 and the cap 20 are held in the mandrel 16 and therefore in the drill-driver 12 by radial mechanical tightening R. The hollow wick 18 is therefore held in the cap 20 by radial mechanical tightening R.

When the rotary motor 14 is started, the rotational movement is transmitted to the hollow wick 18 by the mandrel 16 and through the cap 20.

The hollow wick 18 can for example be made of stainless steel and measure approximately 20 cm (centimeter) long and have an internal diameter of approximately 2 to 4 mm (millimeter) and an external diameter of approximately 3 to 5 mm .

We will now describe a method of collecting a frozen plasma sample by means of the sampling device 10.

Before the first coring, the hollow wick 18 and the cap 20 can be sterile.

Maintaining a frozen plasma bag 22 in a pocket support 24. The pocket support 24 may for example comprise two plates 24A and 24B connected together at their base and parallel to each other. The frozen plasma bag 22 is held by the two parallel plates 24A and 24B (step 100).

Furthermore, the receiving element is positioned in the sampling device. In particular, the cap 20 is positioned in the drill-driver 12, in the mandrel 16 of the drill-driver 12 (step 102).

Then positioning the hollow wick 18 in the cap 20 (step 104).

It is understood that the hollow wick 18 can be positioned in the cap 20 and that the assembly of the hollow wick 18 and the cap 20 can then be positioned in the drill-driver 12.

The mandrel 16 is tightened so that the hollow wick 18 and the cap 20 are mechanically tightened by radial tightening R.

Then positioning the hollow wick 18 in an orifice 26 of the frozen plasma bag 22 (step 106). It is understood that the outside diameter of the hollow wick 18 is less than the inside diameter of the orifice 26. In the example of FIG. 2, the orifice opens onto a tube 28. It is therefore understood that the hollow wick 18 is positioned in the tube 28 before being positioned in the orifice 26.

The hollow wick 18 is rotated by the rotary motor 14 of the drill driver and the frozen plasma is cored by means of the hollow wick 18 in order to take a sample of frozen plasma (step 108).

Then removing the hollow wick 18 from the frozen plasma bag 22 (step 110) and extracting the frozen plasma sample contained in the hollow wick 18 (step 112). The plasma sample taken is placed in a suitable container, for example a plastic tube.

Steps 106 to 112 can be repeated several times with the same hollow wick 18 and the same cap 20 in the same plasma bag 22 and this, until the desired volume of frozen plasma has been taken from the frozen plasma bag 22.

We can then seal the orifice 26 of the frozen plasma bag 22 (step 114) and place the frozen plasma bag 22 in a freezer.

One can also have the frozen plasma bag 22 in a plastic bag which is hermetically sealed by thermal welding. The assembly is then returned to a freezer.

When you want to take frozen plasma from a different bag, you remove the hollow wick 18 and the cap 20 and replace them with a new hollow wick and a new cap, both sterile or clean. This reduces the risk of contamination of the plasma contained in a pocket of frozen plasma by plasma contained in another pocket of frozen plasma, that is to say inter-sample contamination. However, it is advantageously possible to take several samples of frozen plasma sample from a given pocket of frozen plasma with a single hollow wick 18 and a single cap 20.

FIG. 3 represents another embodiment of the frozen plasma bag 22. In FIG. 3, the frozen plasma bag 22 comprises a tube 28 and two chimneys 34 protected by a plastic envelope 32.

Although the present description has been described with reference to a specific embodiment, it is obvious that various modifications and changes can be made to these examples without departing from the general scope of the invention as defined by the claims. In addition, individual features of the various embodiments discussed can be combined in additional embodiments. Therefore, the description and the drawings should be considered in an illustrative rather than restrictive sense.

Claims (9)

1. Device for taking (10) a sample of plasma frozen by coring comprising a rotary motor (14), a hollow wick (18) and an element for receiving the hollow wick, the rotary motor (14) being intended to rotating the hollow wick (18) and the hollow wick (18) being fluidly isolated from the rest of the sampling device (10) by the receiving element. 2. Sampling device (10) according to claim 1, wherein the receiving element is a cap (20), the hollow wick (18) being partially received in the cap (20) and comprising a free end (18B) not received in the cap (20). 3. Sampling device (10) according to claim 2, wherein the free end (18B) of the hollow wick (18) is bevelled. 4. Sampling device (10) according to any one of claims 1 to 3, in which the hollow wick (18) is made of stainless steel. 5. Sampling device (10) according to any one of claims 1 to 4, comprising a drill-driver (12) comprising a mandrel (16), the receiving element being mounted in the mandrel (16). 6. Method for collecting a frozen plasma sample by means of a sampling device (10), the method comprising the following steps: a) holding a pocket of frozen plasma (22) by a pocket support (24); b) positioning of a receiving element in the sampling device (10); c) positioning of a hollow wick (18) in the receiving element, the receiving element fluidly isolating the hollow wick (18) from the rest of the sampling device (10); d) positioning the hollow wick (18) in an orifice (26) of the frozen plasma bag (22); e) rotation of the hollow wick (18) by a rotary motor (14) of the sampling device (10) and coring of the frozen plasma by the hollow wick (18) in order to take a sample of frozen plasma; f) removing the hollow wick (18) from the frozen plasma; and g) extracting the frozen plasma sample from the wick 5 hollow (18). 7. The method of claim 6, wherein steps d) to g) are repeated on the frozen plasma bag (22) without changing the hollow wick (18) and receiving element. 8. The method of claim 6 or 7, wherein, when a volume of frozen plasma collected is at least equal to a predetermined volume, the orifice (26) of the frozen plasma bag (22) is hermetically closed. 9. Method according to any one of claims 6 to 8, in which the frozen plasma bag (22) is a first bag of 15 frozen plasma and, before taking a frozen plasma sample from a second pocket of frozen plasma, different from the first pocket of frozen plasma (22), the hollow wick (18) and the receiving element are changed. 2/3