FR3048285B1 - Procede de nebulisation de proteines pour la generation de reactions immunochimiques, dispositif, utilisation - Google Patents
Procede de nebulisation de proteines pour la generation de reactions immunochimiques, dispositif, utilisation Download PDFInfo
- Publication number
- FR3048285B1 FR3048285B1 FR1651578A FR1651578A FR3048285B1 FR 3048285 B1 FR3048285 B1 FR 3048285B1 FR 1651578 A FR1651578 A FR 1651578A FR 1651578 A FR1651578 A FR 1651578A FR 3048285 B1 FR3048285 B1 FR 3048285B1
- Authority
- FR
- France
- Prior art keywords
- protein
- generation
- nebulization
- nebulization process
- immunochemical reactions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000006243 chemical reaction Methods 0.000 title abstract 3
- 238000002663 nebulization Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 230000000984 immunochemical effect Effects 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000002055 immunohistochemical effect Effects 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B17/00—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups
- B05B17/04—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods
- B05B17/06—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations
- B05B17/0607—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers
- B05B17/0638—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers spray being produced by discharging the liquid or other fluent material through a plate comprising a plurality of orifices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B1/00—Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means
- B05B1/28—Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with integral means for shielding the discharged liquid or other fluent material, e.g. to limit area of spray; with integral means for catching drips or collecting surplus liquid or other fluent material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B17/00—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups
- B05B17/04—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods
- B05B17/06—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations
- B05B17/0607—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers
- B05B17/0653—Details
- B05B17/0676—Feeding means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N2001/317—Apparatus therefor spraying liquids onto surfaces
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Le procédé de réalisation d'une réaction immunohistochimique comprend : ▪ Un agencement d'une préparation d'un échantillon (25) pour le traitement d'une réaction comprenant des antigènes sur un support (20) de sorte à être en vis-à-vis d'une tête de nébulisation (2) ; ▪ Une projection par nébulisation d'une pluralité de gouttelettes (5) correspondant à un volume prédéfini d'un réactif (15) comprenant des anticorps dans une région du support comprenant l'échantillon (25), ladite projection étant générée au moyen d'un élément piézoélectrique (3).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1651578A FR3048285B1 (fr) | 2016-02-25 | 2016-02-25 | Procede de nebulisation de proteines pour la generation de reactions immunochimiques, dispositif, utilisation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1651578A FR3048285B1 (fr) | 2016-02-25 | 2016-02-25 | Procede de nebulisation de proteines pour la generation de reactions immunochimiques, dispositif, utilisation |
Publications (2)
Publication Number | Publication Date |
---|---|
FR3048285A1 FR3048285A1 (fr) | 2017-09-01 |
FR3048285B1 true FR3048285B1 (fr) | 2020-07-31 |
Family
ID=56801625
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR1651578A Active FR3048285B1 (fr) | 2016-02-25 | 2016-02-25 | Procede de nebulisation de proteines pour la generation de reactions immunochimiques, dispositif, utilisation |
Country Status (1)
Country | Link |
---|---|
FR (1) | FR3048285B1 (fr) |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5425918A (en) * | 1990-07-18 | 1995-06-20 | Australian Biomedical Corporation | Apparatus for automatic tissue staining for immunohistochemistry |
DE102006019530B4 (de) * | 2006-04-27 | 2008-01-31 | Bruker Daltonik Gmbh | Probenvorbereitung für massenspektrometrische Dünnschnittbilder |
ES2385851T3 (es) * | 2007-11-20 | 2012-08-01 | Zobele Holding S.P.A. | Dispositivo nebulizador para sustancias líquidas |
FR2948998B1 (fr) * | 2009-08-10 | 2012-10-19 | Assist Publ Hopitaux De Paris | Procede de traitement d'une preparation cytologique ou histologique |
MX2016012022A (es) * | 2014-04-03 | 2017-04-13 | Csl Behring Ag | Nebulizacion de inmunoglobulina. |
-
2016
- 2016-02-25 FR FR1651578A patent/FR3048285B1/fr active Active
Also Published As
Publication number | Publication date |
---|---|
FR3048285A1 (fr) | 2017-09-01 |
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Year of fee payment: 2 |
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Effective date: 20170901 |
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