FR3014445B1 - INSTALLATION FOR COUPLING A BIOREACTOR WITH AN APPARATUS FOR PHYSICO-CHEMICAL ANALYSIS OR COLLECTION OF SAMPLES. - Google Patents

INSTALLATION FOR COUPLING A BIOREACTOR WITH AN APPARATUS FOR PHYSICO-CHEMICAL ANALYSIS OR COLLECTION OF SAMPLES.

Info

Publication number
FR3014445B1
FR3014445B1 FR1362258A FR1362258A FR3014445B1 FR 3014445 B1 FR3014445 B1 FR 3014445B1 FR 1362258 A FR1362258 A FR 1362258A FR 1362258 A FR1362258 A FR 1362258A FR 3014445 B1 FR3014445 B1 FR 3014445B1
Authority
FR
France
Prior art keywords
bioreactor
loop
installation
physico
samples
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1362258A
Other languages
French (fr)
Other versions
FR3014445A1 (en
Inventor
Eric Leclerc
Patrick Paullier
Franck Merlier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Technologie de Compiegne UTC
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Technologie de Compiegne UTC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Technologie de Compiegne UTC filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR1362258A priority Critical patent/FR3014445B1/en
Priority to EP14806236.7A priority patent/EP3077500B1/en
Priority to US15/101,255 priority patent/US9963669B2/en
Priority to PCT/EP2014/076081 priority patent/WO2015082392A1/en
Publication of FR3014445A1 publication Critical patent/FR3014445A1/en
Application granted granted Critical
Publication of FR3014445B1 publication Critical patent/FR3014445B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Thermal Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne une installation de couplage d'un bioréacteur (1) de culture de bio-organismes, en mode dynamique, avec au moins un appareil (2) d'analyse physico-chimique d'un fluide issu de ce bioréacteur ou de collecte d'échantillons de ce fluide, l'appareil (2) étant équipé d'une pompe (20) et d'un injecteur (21), le bioréacteur (1) étant équipé de moyens (10) d'alimentation en milieu de culture et en xénobiotique(s). L'installation est remarquable en ce que le bioréacteur (1) est raccordé à une boucle de perfusion fermée (11) pourvue d'une pompe (12), munie d'une boucle de prélèvement (13) et en ce que l'installation comprend : - un dispositif d'injection d'un produit de nettoyage (6) dans ladite boucle de prélèvement (13), - deux vannes (3, 4) à six voies, la première vanne (3) étant connectée à la boucle de perfusion fermée (11), à la boucle de prélèvement (13) et à une boucle de nettoyage (14), la deuxième vanne (4) étant raccordée à la boucle de nettoyage (14) et à une boucle d'injection (15) qui relie l'injecteur (21) à au moins l'un des appareils (2).The invention relates to an installation for coupling a bioreactor (1) for the cultivation of bio-organisms, in dynamic mode, with at least one device (2) for the physico-chemical analysis of a fluid resulting from this bioreactor or from collection of samples of this fluid, the device (2) being equipped with a pump (20) and an injector (21), the bioreactor (1) being equipped with means (10) for supplying culture and xenobiotic(s). The installation is remarkable in that the bioreactor (1) is connected to a closed perfusion loop (11) provided with a pump (12), provided with a sampling loop (13) and in that the installation comprises: - a device for injecting a cleaning product (6) into said sampling loop (13), - two six-way valves (3, 4), the first valve (3) being connected to the closed infusion (11), to the sampling loop (13) and to a cleaning loop (14), the second valve (4) being connected to the cleaning loop (14) and to an injection loop (15) which connects the injector (21) to at least one of the devices (2).

FR1362258A 2013-12-06 2013-12-06 INSTALLATION FOR COUPLING A BIOREACTOR WITH AN APPARATUS FOR PHYSICO-CHEMICAL ANALYSIS OR COLLECTION OF SAMPLES. Active FR3014445B1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1362258A FR3014445B1 (en) 2013-12-06 2013-12-06 INSTALLATION FOR COUPLING A BIOREACTOR WITH AN APPARATUS FOR PHYSICO-CHEMICAL ANALYSIS OR COLLECTION OF SAMPLES.
EP14806236.7A EP3077500B1 (en) 2013-12-06 2014-12-01 Facility for coupling a bioreactor with a device for physicochemically analysing or collecting samples
US15/101,255 US9963669B2 (en) 2013-12-06 2014-12-01 Facility for coupling a bioreactor with a device for physicochemically analysing or collecting samples
PCT/EP2014/076081 WO2015082392A1 (en) 2013-12-06 2014-12-01 Facility for coupling a bioreactor with a device for physicochemically analysing or collecting samples

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1362258A FR3014445B1 (en) 2013-12-06 2013-12-06 INSTALLATION FOR COUPLING A BIOREACTOR WITH AN APPARATUS FOR PHYSICO-CHEMICAL ANALYSIS OR COLLECTION OF SAMPLES.

Publications (2)

Publication Number Publication Date
FR3014445A1 FR3014445A1 (en) 2015-06-12
FR3014445B1 true FR3014445B1 (en) 2017-03-17

Family

ID=50877366

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1362258A Active FR3014445B1 (en) 2013-12-06 2013-12-06 INSTALLATION FOR COUPLING A BIOREACTOR WITH AN APPARATUS FOR PHYSICO-CHEMICAL ANALYSIS OR COLLECTION OF SAMPLES.

Country Status (4)

Country Link
US (1) US9963669B2 (en)
EP (1) EP3077500B1 (en)
FR (1) FR3014445B1 (en)
WO (1) WO2015082392A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106168630A (en) * 2015-08-21 2016-11-30 福建永晶科技有限公司 A kind of sample injection method utilizing sampling device to realize high-purity sulfur hexafluoride gas
CN105092875A (en) * 2015-09-24 2015-11-25 北京楚翔飞科技开发有限责任公司 Full-automatic gas-liquid multi-purpose sample injector
DE102016121519B4 (en) * 2016-11-10 2019-07-11 Dionex Softron Gmbh System and method for connecting components, in particular in HPLC
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
US4102179A (en) * 1977-04-01 1978-07-25 Phillips Petroleum Company Liquid column chromatographic separation
US4835707A (en) * 1986-07-23 1989-05-30 Takeda Chemical Industries, Ltd. Automatic analysis method and apparatus for enzyme reaction
US5462660A (en) * 1994-04-22 1995-10-31 The United States Of America As Represented By The Secretary Of Agriculture High performance liquid chromatography injection system for the simultaneous concentration and analysis of trace components
ATE217536T1 (en) * 1996-09-25 2002-06-15 Sepiatec Gmbh HPLC-BASED DEVICE AND METHOD FOR SEPARATING HIGHLY COMPLEX SUBSTANCE MIXTURES
US6063284A (en) * 1997-05-15 2000-05-16 Em Industries, Inc. Single column closed-loop recycling with periodic intra-profile injection
US6324924B1 (en) * 1999-04-06 2001-12-04 Roger Peterson Sampling system including a sample additive mixing feature
US6668623B2 (en) * 2000-03-03 2003-12-30 Mitsubishi Materials Corporation Method and apparatus for analyzing organic macromolecular component and application thereof
US7588725B2 (en) * 2001-04-25 2009-09-15 Biotrove, Inc. High throughput autosampler
US20050029196A1 (en) * 2002-11-19 2005-02-10 Resq Lab B.V. Packing materials for separation of biomolecules
US20050019216A1 (en) * 2003-07-24 2005-01-27 Hans-Heinrich Trutnau Apparatus for introducing a sample into a flowthrough analysis system
EP1508791A1 (en) * 2003-08-22 2005-02-23 Ismatec SA, Laboratoriumstechnik Device for automated bioreactor sampling
US8507266B2 (en) * 2003-11-04 2013-08-13 Case Western Reserve University Apparatus and method for tissue engineering
US20060281143A1 (en) * 2005-04-01 2006-12-14 Msp Corporation Method and apparatus for automatic cell and biological sample preparation and detection
US7695957B2 (en) * 2005-07-22 2010-04-13 Silvia Bradamante Bioreactor, in particular for NMR spectroscopy
US7682506B2 (en) * 2005-09-16 2010-03-23 Dionex Corporation IC system including sample pretreatment and using a single pump
CN102187197B (en) * 2008-10-16 2013-04-24 株式会社岛津制作所 Sample injection port, and auto-sampler having the port
FR2957087B1 (en) * 2010-03-02 2014-11-28 Univ Compiegne Tech MULTI-REACTOR BOX FOR DYNAMIC CELL CULTURE
FR2957086B1 (en) 2010-03-02 2014-11-28 Univ Compiegne Tech MULTI-REACTOR BOX FOR DYNAMIC CELL CULTURE

Also Published As

Publication number Publication date
EP3077500A1 (en) 2016-10-12
US9963669B2 (en) 2018-05-08
WO2015082392A1 (en) 2015-06-11
FR3014445A1 (en) 2015-06-12
EP3077500B1 (en) 2017-09-06
US20160304826A1 (en) 2016-10-20

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