FR3002775B1 - Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree - Google Patents

Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree

Info

Publication number
FR3002775B1
FR3002775B1 FR1300467A FR1300467A FR3002775B1 FR 3002775 B1 FR3002775 B1 FR 3002775B1 FR 1300467 A FR1300467 A FR 1300467A FR 1300467 A FR1300467 A FR 1300467A FR 3002775 B1 FR3002775 B1 FR 3002775B1
Authority
FR
France
Prior art keywords
mannanases
mute
catalytic efficiency
improved catalytic
polypeptides encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1300467A
Other languages
English (en)
Other versions
FR3002775A1 (fr
Inventor
Jean Guy Berrin
Marie Couturier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IFP Energies Nouvelles IFPEN
Institut National de la Recherche Agronomique INRA
Agro Industrie Recherches et Developpements ARD
Original Assignee
IFP Energies Nouvelles IFPEN
Institut National de la Recherche Agronomique INRA
Agro Industrie Recherches et Developpements ARD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IFP Energies Nouvelles IFPEN, Institut National de la Recherche Agronomique INRA, Agro Industrie Recherches et Developpements ARD filed Critical IFP Energies Nouvelles IFPEN
Priority to FR1300467A priority Critical patent/FR3002775B1/fr
Priority to US14/771,912 priority patent/US20160102299A1/en
Priority to EP14709545.9A priority patent/EP2986721A1/fr
Priority to PCT/EP2014/000517 priority patent/WO2014131520A1/fr
Priority to CA2902772A priority patent/CA2902772A1/fr
Publication of FR3002775A1 publication Critical patent/FR3002775A1/fr
Application granted granted Critical
Publication of FR3002775B1 publication Critical patent/FR3002775B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01025—Beta-mannosidase (3.2.1.25), i.e. mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C12N9/2494—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01078—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
FR1300467A 2013-03-01 2013-03-01 Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree Active FR3002775B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR1300467A FR3002775B1 (fr) 2013-03-01 2013-03-01 Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree
US14/771,912 US20160102299A1 (en) 2013-03-01 2014-02-27 Polypeptides encoding mutated mannanases with improved catalytic efficiency
EP14709545.9A EP2986721A1 (fr) 2013-03-01 2014-02-27 Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree
PCT/EP2014/000517 WO2014131520A1 (fr) 2013-03-01 2014-02-27 Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree
CA2902772A CA2902772A1 (fr) 2013-03-01 2014-02-27 Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1300467A FR3002775B1 (fr) 2013-03-01 2013-03-01 Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree

Publications (2)

Publication Number Publication Date
FR3002775A1 FR3002775A1 (fr) 2014-09-05
FR3002775B1 true FR3002775B1 (fr) 2016-09-30

Family

ID=48741247

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1300467A Active FR3002775B1 (fr) 2013-03-01 2013-03-01 Polypeptides codant pour des mannanases mutees ayant une efficacite catalytique amelioree

Country Status (5)

Country Link
US (1) US20160102299A1 (fr)
EP (1) EP2986721A1 (fr)
CA (1) CA2902772A1 (fr)
FR (1) FR3002775B1 (fr)
WO (1) WO2014131520A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108559739B (zh) * 2018-05-11 2021-03-26 中国农业科学院北京畜牧兽医研究所 耐热性提高的甘露聚糖酶PMan5A突变体及其基因和应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846705B2 (en) * 2006-07-18 2010-12-07 Direvo Industrial Biotechnology Gmbh Mannanases

Also Published As

Publication number Publication date
EP2986721A1 (fr) 2016-02-24
US20160102299A1 (en) 2016-04-14
CA2902772A1 (fr) 2014-09-04
FR3002775A1 (fr) 2014-09-05
WO2014131520A1 (fr) 2014-09-04

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