FR2988604A1 - Use of fermentation broth of Burkholderia caribensis composition comprising Burkholderia caribensis strain, bacterial culture medium and polysaccharide, for preparing composition containing oligosacchrides with cosmetic properties - Google Patents

Abstract

Use of a composition (A) of the fermentation broth of Burkholderia caribensis comprising Burkholderia caribensis, preferably Burkholderia caribensis strain MWAP71, a bacterial culture medium and polysaccharide (I), produced by the bacteria in the culture medium, for preparing a composition containing oligosacchrides with a molecular weight of 1-30 KDa and cosmetic properties, is claimed. Use of a composition (A) of the fermentation broth of Burkholderia caribensis comprising Burkholderia caribensis, preferably Burkholderia caribensis strain MWAP71, a bacterial culture medium and polysaccharide (I), produced by the bacteria in the culture medium, for preparing a composition containing oligosaccharides with a molecular weight of 1-30 KDa and cosmetic properties, is claimed, where: the concentration of (I) in (A) from fermentation broth is 3.5-20 g/l, preferably 5-15 g/l; and (A) of the fermentation broth is hydrolyzed, hydrolyzed and neutralized, or hydrolyzed, optionally neutralized and purified. Glc : glucose; 6dTal : 6-deoxy-talose; Kdo : 3-deoxy-D-manno-oct-2-ulosonic acid; and n : 700-800. Independent claims are included for: (1) preparing (A) a composition containing oligosaccharides from the fermentation broth of Burkholderia caribensis comprising hydrolyzing (A) of the fermentation broth of Burkholderia caribensis to obtain a hydrolyzate, at a pH of 0-3, preferably 1-2, then thermally hydrolyzing at a temperature of room temperature to 100[deg] C, preferably 80-100[deg] C, thermally acid hydrolyzing at a pH of 0-3, preferably 1-2, and/or at a temperature of room temperature to 100[deg] C, preferably 70-90[deg] C, or enzymatically hydrolyzing in the presence of enzyme comprising proteases and glycosidases, preferably amylases at ambient temperature to 70[deg] C; and (2) the composition comprising the oligosaccharides obtained by the process. [Image] ACTIVITY : Dermatological. MECHANISM OF ACTION : Filaggrin expression stimulator; 3-Aquaporine expression stimulator. The ability of the composition containing oligosacchrides obtained from the fermentation broth of Burkholderia caribensis, preferably Burkholderia caribensis MWAP71 to stimulate filaggrin expression was tested in vitro using normal human keratinocytes. The result showed that the composition containing oligosacchrides exhibited the percentage stimulation of filaggrin of 832 at a concentration of 10 mg/ml of oligosaccharides having 1% dry matter.

Description

The present invention relates to oligosaccharide compositions, to a process for their preparation and to their uses, in particular in cosmetics. The most superficial layer of the skin is called Stratum Corneum and is characterized by the terminal differentiation of keratinocytes from proliferative keratinocytes from the basal layer. The hydration of the stratum corneum is essential for the skin and depends on many factors such as the composition of extracellular lipids (ceramides, cholesterol, free fatty acids) secreted by the lamellar bodies of keratinocytes, the content of NMF (Natural Factors of Hydration) from filaggrin, and also depends on the skin pH gradient, an important element in the general functioning of the Stratum Corneum. All of these factors are altered and diminished with age. In the skin, filaggrin is a marker of late differentiation involved in the hydration and barrier function of the epidermis. The filaggrin filament-aggregating protein interacts with the keratin filaments of the stratum corneum and thus contributes to forming an insoluble keratin matrix (after transglutaminase action) to which the proteins of the horny envelope and lipids can attach. The profilaggrine contained in keratohyaline granules is the precursor of filaggrin. During differentiation profilaggrin is cleaved into filaggrin monomers. Filaggrin has a dual function within the stratum corneum. It catalyzes the formation of disulfide bridges between the keratin filaments and then generates, within the medium and superficial corneocytes, a concentrated pool of free amino acids and derivatives which constitute the NMFs (natural factor of hydration). These offer binding sites for water molecules and establish real bridges between keratin and water, thus maintaining a minimum of hydration of the middle and upper layers of the stratum corneum. Aquaporins (AQP) are a class of membrane proteins that form pores permeable to water and glycerol molecules in biological membranes.

In the skin, aquaporin 3 (AQP3) is localized in keratinocytes. The mice whose aquaporin 3 gene was invalidated show a loss of elasticity of the epidermis, an alteration of the cutaneous barrier as well as a decrease in the quantity of water contained in the stratum corneum at the origin a pronounced dryness of the skin. All these observations argue in favor of a role of aquaporin 3 in the water homeostasis of the epidermis. In addition, one study suggests that aquaporin 3, through its permeability to glycerol and interacting with phospholipase D, could also be involved in the proliferation and differentiation mechanisms of keratinocytes. Thus, an object of the present invention is to provide oligosaccharide compositions for stimulating the expression of filaggrin and / or aquaporin-3. Another object of the invention is to provide compositions comprising oligosaccharides for their use in cosmetics, in particular for improving and / or reinforcing the barrier function and hydration.

The subject of the invention is therefore the use of a fermentation broth composition of Burkholderia caribensis, comprising: bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the Burkholderia caribensis strain MWAP71; culturing said bacteria, and - a polysaccharide-containing composition, produced by said bacteria in said culture medium, said polysaccharides being of the following formula I: ## STR3 ## (3-D-Glcp- (1) -> 4) - (3-D-Glcp- (1-> 4) -α-6dTalp- (1-> 2 (0Ac) 25 in which: Glc represents glucose; 6dTal represents 6-deoxy-talose; Kdo represents 3-deoxy-D-manno-oct-2-ulosonic acid, n is an integer from 700 to 800, the concentration of polysaccharides in said fermentation wort composition ranging from 3.5 to 20 g / L, preferably between 5 and 15 g / L, said fermentation beverage composition being hydrolysed, for the preparation of A composition containing oligosaccharides of molecular weight ranging from 1 to 30 KDa having cosmetic properties Unexpectedly, the Applicant has now found that oligosaccharide compositions, obtainable from fermenting mashes comprising: polysaccharides, bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, and a culture medium of said bacteria, had cosmetic properties, in particular the improvement and / or the reinforcement of the barrier function and hydration. According to an advantageous embodiment, said fermentation must composition is hydrolysed and neutralized. According to another advantageous embodiment, said fermentation must composition is hydrolysed, optionally neutralized and purified. According to a particularly advantageous embodiment, said bacteria are of the strain Burkholderia caribensis MWAP71 ARD, deposited with the CNCM on February 9, 2012 under the number I-4598. The invention also relates to a process for the preparation of a composition containing oligosaccharides with a molecular mass ranging from 1 to 30 KDa, from a fermentation broth composition of Burkholderia caribensis, said fermentation broth composition comprising: bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, a culture medium for said bacteria, and a composition containing polysaccharides, produced by said bacteria in said culture medium, said polysaccharides being of following formula I: -3-Kdop 2 3 → 3) - (3-D-Glcp- (1 → 4) - (3-D-Glcp- (1 → 4) -αL-6dTalp- (1->) 2 in which: (0Ac) Glc is glucose, 6dTal is 6-deoxy-talose, Kdo is 3-deoxy-D-manno-oct-2-ulosonic, n is an integer from 700 to 800 the concentration of polysaccharides in said fermentation must composition ranging from 3.5 to 20 g / L, preferably from 5 to 15 g / L; said method comprising a step of hydrolyzing said Burkholderia caribensis fermentation mash composition to obtain a hydrolyzate. By composition of fermentation wort is meant a composition comprising bacteria, a culture medium suitable for their proliferation and at least one metabolite produced by said bacteria in said culture medium. By "culture medium" is meant an aqueous solution containing nutrients, in particular a carbon source, a nitrogen source, a source of trace elements and a source of growth factors, necessary for bacterial metabolism. In the case of bacteria of the species Burkholderia caribensis, particularly Burkholderia caribensis strain MWAP71, said metabolite is said composition containing polysaccharides. The fermentation wort thus comprises bacteria of the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, the composition containing polysaccharides, and the culture medium at the end of the stage of growth of the bacteria and of producing the polysaccharide-containing composition, i.e., an aqueous solution in which nutrients and metabolites (other than the polysaccharide-containing composition) optionally excreted by the strain are present. By "culture medium suitable for their proliferation" is meant a culture medium adapted to the bacteria of the species in question, in particular to the bacteria of the strain in question, that is to say a culture medium containing the elements nutrients, organic and mineral, necessary for the synthesis of bacterial biomass. Bacterial biomass means the total mass of bacteria present at a given moment in said fermentation must. By hydrolysis is meant a reaction at the end of which at least the main chain of said polysaccharides constituted by the sequence of the [-> 3) -3-D-Glcp- (1-> 4) - (3 -D-Glcp- (1-> 4) -αL-6dTalp- (1->) is cut to obtain oligosaccharides with a molecular mass ranging from 1 to 30 Kda, preferably from 1 to 15 KDa.

Said hydrolysis may for example be acidic, basic, thermal, acidic and thermal, basic and thermal, enzymatic, or enzymatic and thermal. Acidic hydrolysis is understood to mean a hydrolysis reaction carried out in an acidic aqueous medium, said reaction being catalyzed by the H + ions present in said medium.

By basic hydrolysis is meant a hydrolysis reaction carried out in a basic aqueous medium. By thermal hydrolysis is meant a hydrolysis reaction carried out in an aqueous medium, said aqueous medium being heated to a temperature above room temperature.

By enzymatic hydrolysis is meant an enzyme catalyzed hydrolysis reaction. The species Burkholderia caribensis, in particular the Burkholderia caribensis strain MWAP71, was isolated from a vertisol sample collected in the south-east of Martinique Island.

The Burkholderia caribensis strain MWAP71 was classified using molecular genetic tools as belonging to the genus Burkholderia and was filed on 09/02/12 with the CNCM under the Budapest Treaty under registration number I-4598. Said strain was identified after sequencing its rrs gene which codes for 16S ribosomal DNA and by DNA: DNA hybridization as Burkholderia caribensis sp. Nov. 20 This strain has the biochemical characteristics described in Table 1 below: Test Result Status Fresh Gram-negative Gram-negative Gram-negative bacid Oxidase + Catalase + NO3 (nitrate reductase) - N2 (nitrate reductase) - Glucose (ferm) - Arginine dihydrolase - Urease - Hydrolysis of esculin - Gelatinase - Glucose (assimilation) + Arabinose (assimilation) + Mannose (assimilation) + Mannitol (assimilation) + N acetylglucose amine (assimilation) + Maltose (assimilation) - Gluconate (assimilation) + Caprate (assimilation) + Adipate (assimilation) - Malate (assimilation) + Table 1: Biochemical characteristics of the MWAP71 strain On PCA solid medium (Biomérieux France) and after 30 hours of incubation at 30 ° C., the MWAP71 strain forms colonies of white color, type S and a diameter of 5 to 10 mm. PCA (Plate Count Agar) culture medium is well known to those skilled in the art for aerobic total flora counts. Its composition is as follows: peptones 5 g / l, yeast extract 2.5 g / l, glucose 1 g / l, agar 15 g / l. The microscopic observation (x100) of a Gram stain of a 10 MWAP71 colony having been cultured on PCA solid medium (Biomérieux France) for 30 hours at 30 ° C., reports a short gram-negative bacillus. According to an advantageous embodiment, said hydrolysis is acidic. An acid is then added to said fermentation must composition. Said acid is an inorganic or organic acid which is soluble or partially soluble in water. According to another advantageous embodiment, said hydrolysis is thermal. According to a particularly advantageous embodiment, said hydrolysis is thermal and acidic. Said acid is then added to said fermentation must composition and said composition is heated before, during, or after the addition of said acid to a temperature above room temperature. According to an advantageous embodiment, said hydrolysis step, the hydrolysis being acidic or acidic and thermal, is carried out at a pH ranging from 0 to 3, in particular from 1 to 2. According to another advantageous embodiment, said The hydrolysis step, wherein the hydrolysis is thermal, is carried out at a temperature of from room temperature to 100 ° C, preferably at a temperature of from 80 to 100 ° C.

According to another advantageous embodiment, wherein said hydrolysis step, the hydrolysis being acidic and thermal, is carried out at a temperature ranging from room temperature to 100 ° C., preferably at a temperature ranging from 70 to 90 ° C. vs. According to another advantageous embodiment, the hydrolysis is enzymatic.

Enzymes are then added to the fermentation must composition to catalyze the hydrolysis reaction. The enzymatic hydrolysis may be enzymatic and thermal hydrolysis in the case where said composition is heated before, during, or after the addition of said enzymes, to a temperature above room temperature.

According to another advantageous embodiment, the hydrolysis is carried out in the presence of enzymes chosen from the group comprising proteases and glycosidases, in particular amylases. According to a particularly advantageous embodiment, said enzymatic hydrolysis step is carried out at a temperature ranging from room temperature to 70 ° C.

According to another advantageous embodiment, the process according to the invention comprises the following stages: acid hydrolysis and optionally thermal hydrolysing of said fermentation broth composition of Burkholderia caribensis, and neutralization of said hydrolyzate to obtain a neutralized hydrolyzate .

By neutralization, it is meant that the pH is adjusted at the end of the hydrolysis, so that the acid hydrolysis reaction is stopped. According to another advantageous embodiment, the process according to the invention comprises the following steps: - hydrolysis of said fermentation broth composition of Burkholderia caribensis to obtain a hydrolyzate, - optionally neutralization of said hydrolyzate to obtain a neutralized hydrolyzate, and - purification of said hydrolyzate, optionally neutralized, to obtain said composition containing oligosaccharides, with a molecular mass ranging from 1 to 30 KDa.

By purification of the hydrolyzate, it is understood that said hydrolyzate is freed from the bacteria of the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, as well as from the culture medium of said bacteria, that is to say it contains less than 0.01% by mass of bacteria of the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, and / or less than 0.5% by weight of the culture medium. According to another advantageous embodiment, the process according to the invention comprises the following steps: acid and optionally thermal hydrolysis of said fermentation broth composition of Burkholderia caribensis to obtain a hydrolyzate, neutralization of said hydrolyzate to obtain a neutralized hydrolyzate, and purifying said neutralized hydrolyzate to obtain said composition containing oligosaccharides, with a molecular mass ranging from 1 to 30 KDa.

According to another advantageous embodiment, the process according to the invention comprises the following steps: thermal or enzymatic hydrolysis of said composition of fermentation mash of Burkholderia caribensis to obtain a hydrolyzate, purification of said hydrolyzate to obtain said composition containing oligosaccharides with a molecular weight ranging from 1 to 30 KDa. According to a particularly advantageous embodiment, the pH at the end of said neutralization step is from 6 to 8, in particular from 7 to 8.

According to a particularly advantageous embodiment, said purification is by at least one filtration. According to an advantageous embodiment, said purification is done by at least one tangential filtration. According to an advantageous embodiment, said purification comprises the following steps: centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; optionally filtering said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.0 μm, to obtain a first permeate; tangential filtration of said hydrolyzate, optionally neutralized, or said first permeate on a membrane ranging from 10 to 30 KDa, preferably a membrane of 10 KDa, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane of 1 to 5 Kda, preferably a membrane of 1Kda, to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovery of said retentate to obtain said composition containing oligosaccharides of molecular weight ranging from 1 to 30 KDa. By "supernatant, free of said bacteria" is meant that the supernatant contains less than 0.01% by mass of bacteria. According to a particularly advantageous embodiment, said purification comprises the following steps: centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; filtration of said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.01 μm, to obtain a first permeate, tangential filtration of said hydrolyzate, optionally neutralized or said first permeate, on a membrane ranging from at 30 KDa, preferably a membrane of 10 KDa, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane of 1 to 5 Kda, preferably a membrane of 1 Kda, to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovery of said retentate to obtain said composition containing oligosaccharides of molecular weight ranging from 1 to 30 KDa. According to an advantageous embodiment, said composition contains oligosaccharides with a molecular mass ranging from 1 to 15 KDa. According to an advantageous embodiment, the process comprises the following steps: hydrolysis of said composition of fermentation mash of Burkholderia caribensis to obtain a hydrolyzate; optionally neutralization of said hydrolyzate obtained at the end of the preceding step to obtain a hydrolyzate; neutralized hydrolyzate; centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; optionally filtration of said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.01 μm, to obtain a first permeate, tangential filtration of the hydrolyzate, optionally neutralized or of said first permeate, on a membrane ranging from 10 to 30 Kda, preferably a membrane of 10 Kda, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane of 1 to 5 Kda, preferably a membrane of 1Kda , to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovery of said retentate to obtain said composition containing oligosaccharides of molecular mass ranging from 1 to 30 KDa.

According to a particularly advantageous embodiment, the process comprises the following steps: - hydrolysis of said fermentation broth composition of Burkholderia caribensis to obtain a hydrolyzate, - neutralization of said hydrolyzate obtained at the end of the preceding step to obtain a hydrolyzate neutralized, centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; filtration of said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.0 μm, to obtain a first permeate, tangential filtration of the hydrolyzate, optionally neutralized or of said first permeate, on a membrane of from 10 to 30 Kda, preferably a membrane of 10 Kda, to respectively obtain a first or a second permeate, - tangential filtration of said first or second permeate on a membrane ranging from 1 to 15 Kda, preferably a membrane of 1Kda, for obtaining a third permeate comprising the culture medium of said bacteria and a retentate, and recovering said retentate to obtain said composition containing oligosaccharides of molecular weight ranging from 1 to 30 KDa. According to an advantageous embodiment, the process comprises the following steps: - optionally revivification of bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, to obtain revived bacteria, - culture of bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, possibly revived, in a growth medium suitable for their proliferation, to obtain a first fermentation broth composition of Burkholderia caribensis, - optionally inoculation of a fermentor by said first fermentation must composition obtained in the preceding step, and fermentation, during which the fermenter is placed in conditions conducive to the growth of bacteria and to the production of polysaccharides of formula I, to obtain a second composition of must of f erosion of Burkholderia caribensis, - hydrolysis of said first or second fermentation must composition of Burkholderia caribensis to obtain a hydrolyzate, - optionally neutralization of said hydrolyzate obtained at the end of the preceding step to obtain a neutralized hydrolyzate, - centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; optionally filtration of said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.01 μm, to obtain a first permeate, tangential filtration of the hydrolyzate, optionally neutralized or of said first permeate, on a membrane ranging from 10 to 30 Kda, preferably a membrane of 10 Kda, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane ranging from 1 to 5 Kda, preferably a 1Kda membrane, to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovery of said retentate to obtain said composition containing oligosaccharides of molecular mass ranging from 1 to 30 KDa. Said retentate contains less than 0.5% by weight of culture medium. Said fermentation must composition can be obtained by a process comprising the following steps: - optionally revivification of bacteria belonging to the species Burkholderia caribensis, in particular the strain Burkholderia caribensis MWAP71, to obtain revived bacteria, - culture of bacteria belonging to the species Burkholderia caribensis, in particular the strain Burkholderia caribensis MWAP71, possibly revived, in a culture medium suitable for their proliferation, to obtain a first fermentation broth of Burkholderia caribensis comprising a composition containing polysaccharides of formula I, optionally inoculation of a fermentor containing a production medium with said first fermentation wort obtained in the preceding step, and fermentation, during which the fermenter is placed in conditions conducive to the growth of the bacteria and the production of polysaccharides of formula I, to obtain a second fermentation broth of Burkholderia caribensis comprising a composition containing polysaccharides of formula I. By revivification, it is meant to return the bacterial cells in a physiological summer for their growth, thanks to adapted culture conditions , as opposed to their storage conditions, which do not allow their growth, for example cryobank at -80 ° C, or in glycerol. By "production medium" is meant a culture medium for the synthesis of bacterial biomass and the production of polysaccharides within the fermenter.

Although bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the Burkholderia caribensis strain MWAP71, naturally have the capacity to synthesize said polysaccharide-containing composition, they produce only a small amount when they are used. culture on solid medium dishes.

It is therefore essential to develop a culture medium and operating conditions that make it possible to maximize the production of said composition by the species Burkholderia caribensis, in particular of the strain Burkholderia caribensis MWAP71. By "maximizing the production of said composition" is meant to obtain a fermentation must composition in which the concentration of polysaccharide ranges from 3.5 to 20 g / l, preferably between 5 and 15 g / l. The ratio between the carbon and the nitrogen (C / N) present in the culture medium is an important parameter of the behavior of the strain in the face of a deficiency of carbon or nitrogen elements. Advantageously, the choice of a ratio of the mass concentration of the carbon source in the culture medium, for example glucose, on the mass concentration of the nitrogen source in the culture medium, for example a yeast extract , ranging from 7 to 15 makes it possible to maximize the production of the composition containing polysaccharides of formula I. The culture medium is very advantageously composed of yeast extract containing 3 g / l and 23 g / l of glucose, a ratio of 7.6.

According to an advantageous embodiment, the temperature of the production medium varies from ambient temperature to 40.degree. C., in particular from 28.degree. To 32.degree. According to another advantageous embodiment, the pH of the production medium is regulated so as to vary from 6 to 7.5, in particular from 6.5 to 7. According to another advantageous embodiment, the production medium is such that that the partial pressure of oxygen in said medium is greater than or equal to 20%, in particular greater than or equal to 30%. By oxygen partial pressure is meant the oxygen concentration (O 2) dissolved in the liquid phase. This partial pressure is expressed in% of the saturating oxygen concentration in the working conditions under consideration, such as, without limitation, temperature, pressure, agitation. According to an advantageous embodiment, the process comprises the following steps: - revivification of bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, to obtain revived bacteria, - culture of bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, possibly revived, in a culture medium suitable for their proliferation, to obtain a first composition of fermenting must of Burkholderia caribensis, - inoculation of a fermenter by said first fermentation must composition obtained in the previous step, and fermentation, during which the fermenter is placed in conditions conducive to the growth of bacteria and the production of polysaccharides of formula I, to obtain a second composition of must of fermentation of Burkholderia caribensis, - hydrolysis of said first or second fermentation broth composition of Burkholderia caribensis to obtain a hydrolyzate, - neutralization of said hydrolyzate obtained at the end of the preceding step to obtain a neutralized hydrolyzate, - centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; filtration of said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.0 μm, to obtain a first permeate, tangential filtration of the hydrolyzate, optionally neutralized or of said first permeate, on a membrane of from 10 to 30 Kda, preferably a membrane of 10 Kda, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane of 1 to 5 Kda, preferably a membrane of 1Kda, to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovery of said retentate to obtain said composition containing oligosaccharides of molecular weight ranging from 1 to 30 KDa.

According to an advantageous embodiment, said bacteria are of the strain Burkholderia caribensis MWAP71 ARD, deposited with the CNCM on February 9, 2012 under the number I-4598. The invention also relates to a hydrolyzate of a fermentation broth composition of Burkholderia caribensis obtainable by the method described above. The invention also relates to a neutralized hydrolyzate of a fermentation broth composition of Burkholderia caribensis obtainable by the method described above.

The invention also relates to a composition containing oligosaccharides obtainable by the method described above. Said composition corresponds to said hydrolyzate, optionally neutralized, purified, that is to say rid of bacteria of the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, and the culture medium of said bacteria. Said composition may optionally be dissolved in water, for its subsequent use. The solution thus obtained may optionally be discolored, for example with the aid of activated charcoal, in particular by passing through an activated charcoal plate. The solids content of said solution is preferably between 0.1 and 10%, in particular between 0.5 and 2%. The dry matter of said solution can be determined by measuring the mass loss of the sample after passage in the oven. The pH of said solution is preferably between 3 and 8, in particular between 4 and 7. The glucose level of said solution, measured after total acid hydrolysis of the oligosaccharides, by HPLC, is preferably between 0.1 g / l. and 15 g / l, in particular between g / 1 and 10 g / l. The invention also relates to a composition comprising oligosaccharides derived from bacteria of the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, in which: the solids content is preferably between 0.1 and 10. %, in particular between 0.5 and 2%; The pH is between 3 and 8, in particular between 4 and 7; the glucose level of said solution, after total acid hydrolysis of the oligosaccharides, is preferably between 0.1 g / l and 15 g / l, in particular between g / 1 and 10 g / l. The invention also relates to the use of a hydrolyzate of a Burkholderia caribensis fermentation wort composition described above for the preparation of a cosmetic composition. The invention also relates to the use of a neutralized hydrolyzate of a Burkholderia caribensis fermentation must composition described above for the preparation of a cosmetic composition.

The invention also relates to the use of a composition comprising or containing oligosaccharides described above for the preparation of a cosmetic composition. The invention also relates to the use of a composition comprising or containing oligosaccharides described above, for the preparation of a cosmetic composition intended to improve and / or strengthen the barrier function of the skin, as measured according to a loss test. insensitive to water. It is well known to those skilled in the art that an insensitive water loss test is a good indicator of the barrier function of the skin.

The insensitive loss of water can be determined using an evaporimeter, measuring the amount of water evaporated. The invention also relates to the use of a composition comprising or containing oligosaccharides described above, for the preparation of a cosmetic composition intended to improve and / or enhance the hydration of the skin.

It is well known to those skilled in the art that an irritating product will increase the insensible loss of water while a moisturizer will reduce it. The hydration of the skin is thus a biological function related to the barrier function, and is linked to the results of an insensitive water loss test. The invention also relates to the use of a composition comprising or containing oligosaccharides described above, for the preparation of a cosmetic composition intended to improve and / or strengthen the barrier function and the hydration of the skin. The invention also relates to the use of a composition comprising or containing oligosaccharides described above, for improving and / or reinforcing the barrier function of the skin. The invention also relates to the use of a composition comprising or containing oligosaccharides described above, for improving and / or enhancing the hydration of the skin. The invention also relates to the use of a composition comprising or containing oligosaccharides described above, for improving and / or reinforcing the barrier function and hydration of the skin. The invention also relates to the use of a cosmetic composition comprising as active principle a composition comprising or containing oligosaccharides described above.

The cosmetic compositions mentioned above are, for example, solutions, such as lotions, liquid or semi-liquid emulsions such as milks or emulsions of soft consistency such as creams or gels, obtained by dispersion of a fatty phase in an aqueous phase or vice versa, suspensions of liquid, semi-liquid or soft consistency, or powders. These compositions are adapted and prepared according to the usual methods known to those skilled in the art. Among the vehicles used in the usual manner in the cosmetic compositions mentioned above, mention may be made of surfactants, dyes, perfumes, preserving agents, emulsifiers, emulsion stabilizers, emollients, moisturizers, natural or synthetic waxes, thickeners and / or gelling agents, humectants, liquid vehicles such as water, fatty substances such as natural or synthetic oils intended to constitute the fatty phase of milks or creams, and mixtures thereof. The invention also relates to a cosmetic treatment method comprising the topical application of a composition comprising or containing oligosaccharides previously described in a cosmetologically acceptable vehicle.

DESCRIPTION OF THE FIGURES FIG. 1 is a graph showing the evolution of the corrected optical density at 600 nm over time of a preculture medium inoculated with the MWAP71 strain. The corrected optical density corresponds to the optical density at 600 nm (D0600), which is subtracted from the optical density of the initial medium before inoculation: D0600 corrected = D0600 (sample) - D0600 (culture medium before inoculation) Figure 2 is a graph showing the evolution of the corrected optical density at 600 nm (diamonds), the viscosity measured using an RM 100 rheometer from LAMY (module 1, speed 25.8 s-1) (in cps , triangles) and the concentration of glucose (in g / kg, squares) over time, the fermentation must in the fermenter.

EXPERIMENTAL PART The fermenting must composition of Burkholderia caribensis, comprising: bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, a culture medium of said bacteria, and a composition containing polysaccharides produced by said bacteria in said culture medium, said polysaccharides having the following formula I: ## STR3 ## -Glcp- (1-> 4) -α-6dTalp- (1-> 2) (0Ac) wherein: Glc is glucose, 6dTal is 6-deoxy-talose, Kdo is 3-deoxy-deoxy- D-manno-oct-2-ulosonic, n is an integer from 700 to 800, the polysaccharide concentration in said fermentation wort composition ranging from 3.5 to 20 g / L, preferably between 5 and 15 g / L; L 25 can be obtained as illustrated below.The preparation of said fermentation must composition can be be made by biotechnological fermenter. The process then comprises two steps: the production of bacterial cells in small volume vials, this first culture, called pre-culture, then serving to inoculate the actual fermenter where the production of the composition is carried out. The pre-culture chain comprises two steps, the first being the revivification of the bacterial strain stored in a cryobank at -80 ° C and the second a cell proliferation step for the purpose of inoculating the fermenter. The revivification is carried out in 500 ml baffled flasks containing 50 ml of culture medium called "pre-culture medium" whose composition is as follows (Tables 2, 3 and 4): Glucose 10 g / 1 Yeast Extract 3 g / 1 Mineral solution * 70 m1 / 1 Struktol® SB2020 0.1 g / 1 Table 2: Composition of preculture medium Struktol® SB2020 (Schills Seilacher, Hamburg, Germany) is an antifoaming agent. MgSO 4 · 7H 2 O 4 g / 1 FeSO 4 · 7H 2 O 0.44 g / 1 Oligonucleic acid solution ** 20 ml / l Table 3: * Mineral solution H3B03 2.80 g / l MnSO4, H20 1.30 g / l Na2MoO4, 2H20 0.75 g / 1 ZnSO4.7H2O 0.43 g / 1 CoSO4.7H2O 0.070 g / 1 CuSO4 0.023 g / 1 Table 4: * * Solution Trace elements After incubation for 8 hours at 30 ° C. with a stirring of 110 RPM, 7 ml of this culture are transferred to 3-liter baffled flasks containing 500 ml of "pre-culture medium" for the cell proliferation step (production of the inoculum for the fermenter) . These flasks are incubated for 14 h at 30 ° C. with a stirring of 110 RPM. At each transfer, a bacteriological control is carried out by a microscopic observation in the fresh state and by a Gram stain. The growth profile of the MWAP71 strain in pre-culture medium and small volume vials is presented in FIG.

After about 15 hours of growth, that is to say in mid-growth phase, said flasks contain the first composition of fermentation must. Said first fermentation must composition can be used to inoculate a large capacity fermentor: from 150 liters to several tens of cubic meters. Production of the second fermentation must composition can be carried out in a 150L fermentor containing 100L of "production medium" whose composition is as follows (Tables 5, 6 and 7): Glucose 23g / 1 Yeast Extract 3 g / 1 Mineral solution * 70 m1 / 1 Struktol® SB2020 0.1 g / 1 Table 5: Composition of the preculture medium MgSO4,7H20 4 g / 1 FeSO4,7H20 0.44 g / 1 Oligonosal solution ** 20 ml / 1 Table 6: * Mineral solution H3B03 2.80 g / 1 MnSO4, H2O 1.30 g / 1 Na2MoO4.2H2O 0.75 g / 1 ZnSO4.7H2O 0.43 g / 1 CoSO4.7H2O 0.070 g / 1 CuSO4 0.023 g / 1 Table 7: * * Solution Trace elements The culture medium without glucose is sterilized in place in the fermenter, then a concentrated solution of glucose sterilized separately by the action of heat is added sterilely to obtain the desired initial glucose concentration (23 g / l).

Inoculation of the fermenter is carried out by sterile transfer of the contents of 4 to 8 pre-culture flasks, ie an inoculation rate of 2 to 4% vol / vol. The culture conditions applied during fermentation are as follows: - Temperature: 30 ° C - pH: 6.5 to 7.0 regulated by addition of 6% NaOH - pO 2> 30% In order to maintain sufficient oxygenation of the medium (pO 2> 30%), the aeration of the fermenter is maintained between 0.5 and 1 VVM, the stirring is between 200 and 500 RPM (ie between 1.7 and 4.3 m / s) and a cover pressure is maintained between 0.5 and 1 bar. During production, monitoring of bacterial biomass production is performed by measuring the optical density at 600 nm (D0600), which is subtracted from the optical density of the initial medium before inoculation: D0600 corrected = D0600 (sample) - D0600 (culture medium before inoculation) The monitoring of the polymer production is carried out by measuring the viscosity of the fermentation must by a Rhéomat RM 100 rheometer from the company LAMY (module 1, speed 25.8 s-1) . Finally, the glucose consumption is monitored by assaying on a YSI 2700 SELECT glucose analyzer from YSI Life Sciences. When the residual glucose concentration reaches zero, the production is complete. The evolution of the fermentation must over time is illustrated in FIG. 2. The increase in the viscosity of the culture medium reflects the appearance within the fermenter of the composition containing the polysaccharides produced by the strain. When glucose is completely consumed, fermentation is considered complete. At the end of this fermentation process on a 150 liter fermentor, the second fermentation must composition comprising the nutrients not consumed by the strain, the bacterial biomass of the MWAP71 strain and the metabolites produced by the strain are obtained. strain whose composition contains the polysaccharides. At the end of the industrial fermentation step, the following results are obtained (Table 8): Duration of the fermentation 33.5 hrs Duration of the phase of production of polysaccharides 16 5 h max (growth rate of the strain) 0.3 dS / dt yarn Average glucose consumption 0.7 g / l / h dP / dt Average polysaccharide productivity 60 mPa.s / h Final polysaccharide concentration 4.3 g / 1 Final concentration in 12 g cells Table 8: Results of the production of the second fermentor fermentation must composition.

The composition and characteristics of the fermentation must are as follows (Table 9): Must dry matter 1.65% MS pH 6.5-7.0 OD 600nm final 41.4 Final viscosity (26 s1) 1030 mPa.s Table 9: Composition and characteristics of the fermentation must containing the native polymer.

Examples 1 to 4 which follow illustrate the invention. EXAMPLES Example 1 Preparation of the Composition Containing Oligosaccharides The composition containing oligosaccharides is obtained according to the following process: acid and thermal hydrolysis of the second composition of fermentation broth for 5 hours at 80 ° C. to pH 1.6 by addition of hydrochloric acid; neutralization at pH = 7.4 with sodium hydroxide and cooling at 20 ° C .; centrifugation at 4080 g for at least 3 minutes to separate the bacterial biomass from the oligosaccharides; filtration of the centrifugation supernatant on a cellulose slab of porosity 0.3-1.0 μm; - Tangential filtration on a 10 Kda surface membrane of 1.02 m2 with a transmembrane pressure (PTM) of 1 bar and permeate recovery; - Tangential filtration on a 1 Kda surface membrane of 1.02 m2 with a transmembrane pressure (PTM) of 1 bar and recovery of the retentate; - discoloration of the retentate by passage on a plate of activated carbon; - dilution of the product to about 1% dry matter. The composition containing oligosaccharides (active ingredient) is obtained in the form of an aqueous solution having the following characteristics: pH: 6.86 (measured by potentiometric method at room temperature).

Dry matter: 1.06% (the dry matter can be determined by measuring the mass loss of the sample in the presence of fountain sand after passing in the oven 105 ° C for 12 hours). Glucose level: 5 g / l (measured after total acid hydrolysis of the oligosaccharides, by HPLC, the total hydrolysis can be obtained by adding TFA, so as to obtain a 4M solution in TFA, then heating at 100 ° C. for 4 hours). Example 2 In Vitro Studies Normal human keratinocytes are cultured in a monolayer for 5 days (37 ° C. and 5% CO 2) in the presence of a culture medium comprising 10 mg of the 1% solids oligosaccharide solution ( Example 1), per ml of culture medium, or culture medium alone for the control conditions. The expression of filaggrin is evidenced by immunostaining (ICC chemical immuno cyto) using an anti-filaggrin monoclonal antibody (Filaggrin (AE21) Santa Cruz Biotechnology) directed and purified against human hair filaggrin. This antibody is described for applications of detection of filaggrin of human origin in immunofluorescence and immunohistochemistry. This primary antibody is associated with a secondary antibody for FITC fluorescence signal detection.

Expression of aquaporin-3 is evidenced by immunostaining (ICC chemical immuno-cyto) using a polyclonal anti-AQP3 antibody (AQP 3 (H-80) Santa Cruz Biotechnology) specifically directed and purified against AQP3. human origin at the N-terminal portion of the protein. This antibody is described for applications of detection of AQP3 in immunofluorescence, western blot, immunoprecipitation and Elisa.

This primary antibody is associated with a secondary antibody for detection of the fluorescence signal Rhodamine type. Quantification of the labeling intensity of fillagrin and aquaporin 3 in normal human keratinocytes induced by oligosaccharides derived from Burkholderia caribensis is carried out using the Histolab image analysis software from the company Mi crovi if we. The results obtained on the expression of filaggrin are shown in Table 10: Control 10 mg / ml solution of oligosaccharides at 1% dry matter Intensity (IU) 362 3888 Standard deviation 101 832 p / control 0.0057 Table 10 : Expression of filaggrin in the presence or absence of the composition containing the oligosaccharides. The composition containing the oligosaccharides of Burkholderia caribensis significantly stimulates the expression of filaggrin. The results obtained on the expression of aquaporin-3 are shown in Table 11: Control 10 mg / ml solution of oligosaccharides at 1% dry matter Intensity (UI) 2077 14419 Average 265 3140 p / control 0.017 Table 11 : Expression of aquaporin-3 in the presence or absence of the composition containing the oligosaccharides. The composition containing the oligosaccharides of Burkholderia caribensis significantly stimulates the expression of aquaporin-3. Example 3 In Vivo Test Two emulsion formulations are prepared, one constituting the placebo and the other according to the invention, constituting the formulation comprising oligosaccharides. The composition of both formulations is reported in Table 12: Emulsion CMWA 10-0 Emulsion CMWA 10-1 (placebo) (invention) Xyliance 5% 5% Caprylic capric triglycerides 12% 12% Phenonip XB 0.4% 0, 4% 1% aqueous solution (dry matter) of Burkholderia caribensis oligosaccharides 0% 1% Aqua QSP 100% QSP 100% Table 12: composition of the two formulations used in the in vivo test. The study is performed on 19 healthy male and female volunteers with normal skin in the arms. Insensitive water loss measurements (PIE) are performed with a Vapometer® (Delphin) equipped with a probe that measures the gradient of water vapor set up between the skin surface and the ambient air. This measure gives information on the quality of the barrier function of the skin. The protocol of the study is described below: - three days before the start of the study, the volunteers do not apply any cream in the arms; two zones are defined on the forearms, one zone that will be treated with the formulation containing oligosaccharides of Burkholderia caribensis and the other zone that will be treated with the formulation that does not contain the active ingredient (placebo); the PIE is measured using the Vapometer® at Jl / TO; - On day 1, the skin area defined on each forearm is subjected to a specific treatment by the Stripping technique using the adhesive disc applicator (D.Squame® piston, ref Monaderm PDSQ GOA). stripping makes it possible to induce a slight decrease in the natural barrier effect of the superficial layers of the epidermis (stratum corneum) by removing a few cells at the surface, in contact with the adhesive; the PIE is then measured using the Vapometer® just after stripping (J1 / Immediate); the formulations are applied to their specific zones; the formulations are applied as such, by the technician, using a fingernail by gentle digital massage until absorption on a skin surface delimited by about 16 cm 2, at a rate of 2 mg / cm 2; the IEM is remeasured one hour after application of each formulation (J1 / T1h); the formulations are applied twice a day for 3 days; at J3, the operation is repeated. The effects of the two formulations were compared with one another at times (Ji / Tlh (Ji / Immediate-Ji / TO)). The comparison of the area data processed by each formulation is done by a Paired Pair Student or Wilcoxon Rank Matched Series Test (in case of non-normal data distribution). The results are given in the table below (Table 13): Day 1 Day 3 APTE = J1 / T1h- (J1 / T APTE = J3 / T1h- (J3 / T immediate-J1 / TO)) immediate-J1 / TO)) (g / m2.h) (g / m2.h) Cream CMWA 10-0 (placebo) 1,2 3,4 Cream CMWA 10-1 0,4 -0,1 P = 0,641 0,039 Table 13: results of the in vivo test The repairing effect obtained with the product Emulsion CMWA 10-1 is greater after 3 days of repeated application on skin stripped compared to that obtained with the product Emulsion CMWA 10-0. As a result, Burkholderia caribensis Oligosaccharides can significantly repair the skin barrier.

Example 4: Formulas Example 4.1: Moisturizing cream for the body The cream of the following formula (Table 14): 25 Phase Ingredient% A Xyliance (Soliance) 4 Macadamia ternifolia seed oil 6 Kendi oil 0.5 Preservative QS B Aqua qs 100 Soligel (Soliance) 0.1 Sodium Hyaluronate 2 C 1% aqueous solution (dry matter) in Burkholderia caribensis oligosaccharides 1 D Manuka honey 0.5 Table 14: Moisturizing body cream formula is obtained as follows: 1. Prepare separately A and B, under stirring and at 70 ° C. 2. Gently add B to A under foaming at 3000 rpm. 3. Cool. 4. At 40 ° C add C and D with slow stirring at 40 rpm. Example 4.2: Day cream The cream of the following formula (Table 15): Phase Ingredient% A Xyliance (Soliance) 3.5 Ore oil 2 Caprylic / capric triglyceride 3 Isostearyl isostearate 3 Dimethicone 2 Prunus armeniaca (Apricot) Kernel oil 2 B Glyceryl stearate citrate 1.5 Preservative Qs Aqua qs 100 Glycerin 2 Xanthan gum 0.7 C 1% aqueous solution (dry matter) of Burkholderia caribensis oligosaccharides 1 D Perfume qs Table 15: formula of a day cream is obtained as follows: 1. Prepare separately A and B, with stirring and at 70 ° C. 2. Gently add B to A under foaming at 3000 rpm. 3. Cool. 4. At 40 ° C add C and D with slow stirring at 40 rpm. Example 4.3: Facial gel gel The following formula gel (Table 16): Phase Ingredient% A Aqua qs 100 Tourmaline (Soliance) 1 Carbomer 0.7 Hydroxyethylcellulose 0.3 Preservative Qs B Sodium Hydroxide Qs pH 7 C Aqueous solution at 1% (dry matter) in Burkholderia caribensis 1 Betaphroline 2 oligosaccharides Table 16: Face gel gel formula is obtained as follows: 1. Weigh A and heat with slow stirring at 70 ° C in a Bain-Marie; 2. Adjust the pH to 7 with B; 3. Add C to AB with slow stirring. Example 4.4: Relaxing moisturizing gel The gel of the following formula (Table 17): Phase Ingredient% A Porphyraline (Soliance) qs 100 Hydroxyethylcellulose 1 B Grevilline (Soliance) Betaphroline 10 1% aqueous solution (dry matter) of Burkholderia oligosaccharides caribensis 1 Spring sea water 10 C Polysorbate 20 1.36 Perfume qs Preservative qs D CI dye 17200 qs CI dye 42090 qs Table 17: Formula of a relaxing hydrating gel is obtained as follows: 1. Weigh A and heat to 70 ° C under agitation in a Bain-Marie; 2. Allow to cool to room temperature; 3. At 40 ° C, add successively the compounds of B and stir between each addition; 4. Weigh C and mix until perfect homogenization; 5. At 35 ° C, add C then D and mix until homogenous.

Claims (13)

REVENDICATIONS1. Use of a fermentation broth composition of Burkholderia caribensis, comprising: - bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, - a culture medium of said bacteria, and - a composition containing polysaccharides produced by said bacteria in said culture medium, said polysaccharides being of the following formula I: ## STR5 ## - (3-D-Glcp- (1-> 4) - (3-D- Glcp- (1-> 4) -α-6dTalp- (1-> 2 (OAc) n I wherein Glc is glucose, 6dTal is 6-deoxy-talose, Kdo is 3-deoxy- D-manno-oct-2-ulosonic, n is an integer from 700 to 800, the concentration of polysaccharides in said fermentation wort composition ranging from 3.5 to 20 g / L, preferably from 5 to 15 g Wherein said fermentation beverage composition is hydrolysed for the preparation of an oligosac-containing composition chars having a molecular weight ranging from 1 to 30 KDa having cosmetic properties, especially a use wherein said fermentation wort composition is hydrolysed and neutralized, especially a use wherein said fermentation wort composition is hydrolysed, optionally neutralized and purified . 2. Use according to claim 1, wherein said bacteria are of the strain Burkholderia caribensis MWAP71 ARD, filed with the CNCM on February 9, 2012 under the number I-4598. 15 3. Process for the preparation of a composition containing oligosaccharides with a molecular mass ranging from 1 to 30 KDa, from a fermentation broth composition of Burkholderia caribensis, said fermentation broth composition comprising: bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, - a culture medium of said bacteria, and - a composition containing polysaccharides, produced by said bacteria in said culture medium, said polysaccharides having the following formula I: 3-D-Glcp- (1-> 4) - (3-D-Glcp- (1-> 4) -αL-6dTalp- (1-> 2 (0Ac) wherein: Glc is glucose, 6dTal is 6-deoxy-talose, Kdo is 3-deoxy-D-manno-oct-2-ulosonic, n is an integer from 700 to 800, and the concentration is polysaccharides in said fermentation wort composition ranging from 3.5 to 20 g / L, from ference between 5 and 15g / L; said method comprising a step of hydrolyzing said Burkholderia caribensis fermentation mash composition to obtain a hydrolyzate, in particular a process wherein said hydrolysis is: - acid, said hydrolysis step being in particular carried out at a pH ranging from 0 to 3, more particularly 1 to 2, or - thermal, said hydrolysis step being in particular carried out at a temperature ranging from room temperature to 100 ° C., more particularly at a temperature ranging from 80 to 100 ° C. and / or thermal and acidic, said hydrolysis step being in particular carried out at a pH ranging from 0 to 3, more particularly from 1 to 2, and / or at a temperature ranging from room temperature to 100 ° C., more particularly at a temperature ranging from 70 to 90 ° C, or - enzymatically, said hydrolysis step being in particular carried out in the presence of enzymes chosen from the group comprising proteases and glycosides ases, more particularly the amylases, and / or at a temperature ranging from room temperature to 70 ° C. 4. Method according to claim 3, comprising the following steps: - hydrolysis of said composition of fermenting mash Burkholderia caribensis to obtain a hydrolyzate, - optionally neutralization of said hydrolyzate to obtain a neutralized hydrolyzate, and - purification of said hydrolyzate, optionally neutralized, to obtain said composition containing oligosaccharides, with a molecular mass ranging from 1 to 30 KDa, in particular a process comprising the following steps: acid and optionally thermal hydrolysis of said composition of fermentation mash of Burkholderia caribensis to obtain a hydrolyzate, neutralization of said hydrolyzate to obtain a neutralized hydrolyzate, in particular a neutralization at the end of which the pH ranges from 6 to 8, in particular from 7 to 8, and - purification of said neutralized hydrolyzate to obtain said composition containing oligosaccharides, with a molecular mass ranging from 1 to 30 KDa, especially a p wherein said purification is by at least one filtration, particularly tangential filtration, including a method wherein said purification comprises the steps of: centrifuging said hydrolyzate to obtain a supernatant, free of said bacteria; optionally filtering said supernatant, optionally neutralized, on a cellulosic plate, preferably of porosity 0.3-1.4m, to obtain a first permeate, tangential filtration of said hydrolyzate, optionally neutralized or said first permeate, on a membrane ranging from 10 to 30 KDa, preferably a membrane of 10 KDa, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane of 1 to 5 Kda, preferably a membrane of 1Kda, to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovery of said retentate to obtain said composition containing oligosaccharides of molecular weight ranging from 1 to 30 KDa, in particular from 1 to 15 KDa. 5. Method according to any one of claims 3 to 4, comprising the following steps: - hydrolysis of said fermentation broth composition of Burkholderia caribensis to obtain a hydrolyzate, - optionally neutralization of said hydrolyzate obtained at the end of the step previous to obtain a neutralized hydrolyzate, - centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; optionally filtration of said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.01 μm, to obtain a first permeate, tangential filtration of the hydrolyzate, optionally neutralized or of said first permeate, on a membrane ranging from 10 to 30 Kda, preferably a membrane of 10 Kda, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane ranging from 1 to 5 Kda, preferably a 1Kda membrane, to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovery of said retentate to obtain said composition containing oligosaccharides of molecular mass ranging from 1 to 30 KDa, in particular a process comprising the following steps: hydrolysis of said composition fermentation broth of Burkholderia caribensis to obtain a hydrolyzate, - neutralization of the said hydrolyzate obtained at the end of the preceding step to obtain a neutralized hydrolyzate, centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; filtration of said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.01 μm, to obtain a first permeate, tangential filtration of the hydrolyzate, optionally neutralized or of said first permeate, on a membrane ranging from 10 to 30 Kda, preferably a membrane of 10 Kda, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane of 1 to 5 Kda, preferably a membrane of 1Kda, for obtaining a third permeate comprising the culture medium of said bacteria and a retentate, and recovering said retentate to obtain said composition containing oligosaccharides of molecular weight ranging from 1 to 30 KDa. 6. Process according to any one of claims 3 to 5, comprising the following steps: - optionally revivification of bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, to obtain revivified bacteria, cultivation of bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, possibly revived, in a culture medium suitable for their proliferation, to obtain a first fermentation must composition of Burkholderia caribensis, optionally inoculation of a fermentor by said first fermentation must composition obtained in the preceding step, and fermentation, during which the fermenter is placed in conditions conducive to the growth of bacteria and to the production of polysaccharides of formula I, for get a second composition of must from fermentation of Burkholderia caribensis, - hydrolysis of said first or second composition of fermentation mash of Burkholderia caribensis to obtain a hydrolyzate, - optionally neutralization of said hydrolyzate obtained at the end of the preceding step to obtain a neutralized hydrolyzate, - centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; optionally filtering said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.0 μm, to obtain a first permeate, tangential filtration of the hydrolyzate, optionally neutralized or said first permeate, on a membrane ranging from 10 to 30 Kda, preferably a 10Kda membrane, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane of 1 to 30 Kda, preferably a membrane of 1Kda to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovery of said retentate to obtain said composition containing oligosaccharides of molecular mass ranging from 1 to 30 KDa, in particular a process comprising the following steps: - revivification of bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the Burkh strain olderia caribensis MWAP71, to obtain revived bacteria, - culture of bacteria belonging to the species Burkholderia caribensis, in particular bacteria of the strain Burkholderia caribensis MWAP71, possibly revived, in a culture medium suitable for their proliferation, to obtain a first fermentation broth composition of Burkholderia caribensis, - inoculation of a fermentor by said first fermentation must composition obtained in the previous step, and fermentation, during which the fermentor is placed under conditions conducive to the growth of bacteria and the production of polysaccharides of formula I, to obtain a second composition of fermentation broth of Burkholderia caribensis, - hydrolysis of said first or second fermentation broth composition of Burkholderia caribensis to obtain a hydrolyzate, - neutralization of said hydrolyzate obtained at the end of the preceding step to obtain a neutralized hydrolyzate; centrifugation of said hydrolyzate to obtain a supernatant, freed of said bacteria; filtration of said supernatant, optionally neutralized, on a cellulosic plate, preferably with a porosity of 0.3-1.01 μm, to obtain a first permeate, tangential filtration of the hydrolyzate, optionally neutralized or of said first permeate, on a membrane ranging from 10 to 30 Kda, preferably a membrane of 10 Kda, to obtain respectively a first or a second permeate, tangential filtration of said first or second permeate on a membrane ranging from 1 to 5 Kda, preferably a 1Kda membrane, to obtain a third permeate comprising the culture medium of said bacteria and a retentate, and recovering said retentate to obtain said composition containing oligosaccharides of molecular weight ranging from 1 to 30 KDa. 7. Method according to any one of claims 3 to 6, wherein said bacteria are Burkholderia caribensis strain MWAP71 ARD, filed with the CNCM on 09 February 30, 2012 under the number I-4598. 8. Composition containing oligosaccharides obtainable by the process according to any one of claims 3 to 6. 9. A composition comprising oligosaccharides derived from bacteria of the species Burkholderia caribensis in which: the solids content is preferably between 0.1 and 10%, in particular between 0.5 and 2%; the pH is between 3 and 8, in particular between 4 and 7; the glucose level of said solution, after total acid hydrolysis of the oligosaccharides, is preferably between 0.1 g / l and 15 g / l, in particular between g / 1 and 10 g / l. 10. Use of a composition comprising or containing oligosaccharides according to claim 8 or 9 for the preparation of a cosmetic composition, in particular: a cosmetic composition intended to improve and / or strengthen the barrier function of the skin, such as as measured according to an insensitive water loss test, or - a cosmetic composition intended to improve and / or reinforce the hydration of the skin, or - a cosmetic composition intended to improve and / or reinforce the barrier function and hydration of the skin. 11. Use of a composition comprising or containing oligosaccharides according to claim 8 or 9 for: - improving and / or reinforcing the barrier function of the skin, and / or - improving and / or enhancing the hydration of the skin. 12. Cosmetic composition comprising as active principle a composition comprising or containing oligosaccharides according to claim 8 or 9. 13. Cosmetic treatment method comprising the topical application of a composition comprising or containing oligosaccharides according to claim 8 or 9 in a cosmetologically acceptable vehicle. 30