FR2905857A1 - Cosmetic process, useful to care human skin, to hydrate and/or protect against dryness, comprises topical application of composition containing an extract of carob (Ceratonia siliqua) pulp on the skin - Google Patents

Abstract

Cosmetic process for caring human skin, to hydrate and/or to protect against dryness, comprising topical application of a composition containing an extract of carob ( Ceratonia siliqua) pulp on the skin. Independent claims are included for: a cosmetic use of the extract for hydrating the human skin and/or protect against drying; #a cosmetic composition comprising, in a medium, the extract and at least an active ingredient such as keratolytic agents, agents increasing the differentiation and/or cornification of keratinocytes, epidermal lipids and agents increasing the synthesis of epidermal lipids, humectants, agents facilitating percutaneous absorption and/or fillers; and #a carob ( Ceratonia siliqua) extract, where the extract is obtained from extraction of carob pulp with the help of at least an alcoholic solvent.

Description

1 Using a carob pulp extract to moisturize the skin The

  The present invention relates to a cosmetic process for the care of human skin, intended to moisturize and / or protect it from drying, comprising the topical application to the skin of a composition containing a pulp extract. carob (ceratonia siliqua).

  The skin consists mainly of three layers, namely, starting from the most superficial, the epidermis, the dermis and the hypodermis.

  The epidermis consists in particular of keratinocytes (majority), melanocytes (involved in the pigmentation of the skin) and Langerhans cells. Its function is to protect the body from the external environment and to ensure its integrity, in particular to slow the penetration of micro-organisms or chemical substances, and to prevent the evaporation of the water contained in the skin. For this purpose, the keratinocytes undergo a continuous oriented maturation process in which the keratinocytes located in the basal layer of the epidermis form, at the end stage of their differentiation, corneocytes which are dead cells totally keratinized in the form of envelopes. corneas consisting of proteins and lipids such as ceramides. During this differentiation process, epidermal epidermal lipids are furthermore formed and then organized in the form of bilayer 2905857 2 (layers) in the stratum corneum, which participate, with the above-mentioned horny envelopes, in the barrier function of the epidermis.

5 The barrier function of the epidermis may, however, be disturbed under certain climatic conditions (under the effect of cold and / or wind, for example), or under the effect of stress or fatigue, in particular, favoring and the penetration of allergens, irritating agents or micro-organisms which thus cause skin dryness likely to generate feelings of discomfort such as tightness or redness, and also to alter the radiance of the complexion and the suppleness of the skin.

To prevent this phenomenon or to correct it, it is known to apply to the skin cosmetic compositions containing hygroscopic agents, such as sugars or polyols, intended to capture the water present in the skin and thus to slow down its evaporation. It is also conventionally resorted to fatty substances for forming an occlusive film on the skin, which helps to slow the evaporation of water. Moreover, these compositions frequently incorporate active agents acting on one or more of the different biological targets involved either in the regeneration processes of the skin, in particular in the differentiation of keratinocytes, the synthesis of epidermal lipids and the cohesion of corneocytes, or In the endogenous synthesis of constituents of the natural Moisturizing Factor (NMF) of the skin, particularly in the synthesis of proteoglycans.

Examples of such active agents include α- and R-hydroxy acids, especially lactic acid, glycolic acid and salicylic acid; urea; or aminosulfonic compounds. However, there is still the need to propose new cosmetic active agents to fight more effectively cutaneous dryness.

In addition, in view of the ever-increasing search by consumers for natural products containing the least possible synthetic ingredients, and the ever-increasing regulatory burden on compounds derived from the chemical industry, it would be desirable that these cosmetic assets are of plant origin. Now, the Applicant has had the merit of showing that it is possible to act topically on a new biological target, namely the matriptase MT / SP1, to effectively fight against drying of the skin, by using an extract botanical, namely an extract of carob pulp (ceratonia siliqua).

Matriptase MT / SP1 (also designated ST14 and TAGD-15) is a type II transmembrane protease widely expressed in most cells of epithelial origin and in particular by keratinocytes. Recent mouse studies have shown that ablation of the MT / SP1 matriptase results in stratum corneum malformations, which exhibited a more compact appearance and less organized stratification, compromising the epidermal barrier function and ultimately resulting in the death of the mouse by dehydration. This protease is moreover known to be expressed in a wide variety of human tumors of epithelial origin, as well as during cicatrization and in skin diseases such as ichthyosis. In the aforementioned study, the mouse is thus used as a model for the study of these pathologies (K. List et al., Oncogene, 2002; 21: 3765-3779). A more recent study (List et al., J. Cell Biol., 2003; 163: 901-910) has shown that MT / SP1 matriptase was a key enzyme in epidermal end differentiation and that the matriptase deficient mouse was a good model for studying severe human ichthyosis of the so-called Harlequin type.

However, to the knowledge of the Applicant, it has never been disclosed cosmetic active, a fortiori of botanical origin, stimulating the expression of MT / SP1 matriptase.

In addition, it is known to use in the food and cosmetic field locust bean gum (Ceratonia siliqua), or carob seed flour, as a gelling and / or film-forming agent, preferably in combination with carrageenans. guar or xanthan gum. This gum> consists of a polysaccharide, an almost pure D-galacto-D-mannan. It has furthermore been suggested that the seeded fruits of locust bean could, after an aqueous alkaline hydrolysis treatment (A. De Leonardis et al., La Rivista Italiana Delle Sostanze Grasse, Vol.LXXX, Nov.-Dec. ) or after extraction 2905857 (hydro) alcoholic (EP-0 131 176) a source of antioxidants used in food or in cosmetics. Locust bean pulp is also used to make molasses or cocoa substitutes.

In the pharmaceutical field, it is sometimes used as a decoction to make anti-diarrheal preparations. The present invention thus relates to a cosmetic process for the care of human skin, intended to moisturize and / or protect it from drying, including the topical application to the skin of a composition containing an extract of carob pulp.

The present invention also relates to the cosmetic use of a carob pulp extract to promote the formation of a functional barrier and allow better hydration of human skin and / or protect it from drying out. , Advantageously by stimulating the expression of MT / SP1 matriptase in the epidermis. The present invention also relates to a carob extract characterized in that this extract is obtainable by extraction of the carob pulp with at least one alcoholic solvent. The carob pulp extract which can be used in the present invention may be derived from locust beans, optionally dried and preferably ground. These pods are advantageously seeded before their implementation.

This extract can be obtained using any solvent and preferably using an alcoholic solvent. By this expression is meant an extraction solvent comprising at least one monoalcohol, such as methanol, ethanol or isopropanol, and / or at least one glycol, such as propylene glycol or dipropylene glycol. , possibly in the presence of water. The extraction is generally carried out by immersing or gently stirring the raw material used, advantageously the ground material, in one or more of the solvents mentioned above at temperatures ranging, for example, from room temperature to 100.degree. and advantageously from 40 to 80 ° C, for a period of about 15 minutes to 50 hours, and preferably from 20 to 40 hours. The solvent / material ratio may for example be between 1: 1 and 100: 1 and preferably between 10: 1 and 50: 1.

The resulting solution is then generally filtered to remove insoluble substances from the plant. If necessary, the solvent is also removed if it is a volatile solvent such as, for example, ethanol, methanol or isopropanol. This extraction step is usual in the field of plant extracts, and the person skilled in the art is able to adjust the reaction parameters on the basis of his general knowledge. At the end of this extraction step, a carob oleoresin is obtained.

The oleoresin may then, in an advantageous aspect, be subjected to a bleaching step, in particular using activated carbon in the presence of a solvent. This bleaching operation comprises contacting the extract obtained after the solvent extraction with active charcoal in a suitable solvent. The weight of activated carbon added is preferably between 0.5% and 50% of the weight of the extract. For example, one or more solvents selected from water, C1-C4 alcohols such as methanol, ethanol or isopropanol, polar organic solvents such as propylene glycol or dipropylene glycol, or any another common solvent in the field. The volatile solvents can then be removed under reduced pressure. The carob pulp extract used according to the invention can be used in a proportion of 0.00001 to 10% by weight, preferably in a proportion of 0.0001 to 5% by weight, more preferably in a proportion of 0.001 to 1% by weight. weight, better, at a rate of 0.01 to 0.1% by weight, relative to the total weight of the composition.

Locust bean pulp extract is used according to the invention for cosmetic purposes, to moisturize or protect human skin from drying. The inventors have demonstrated that this effect was achieved by an improvement in the barrier function. The method according to the invention can therefore be used to fight against the cutaneous signs resulting from a disturbed barrier function, including the roughness of the skin, the discomforts including redness, tingling, tingling and irritation. itching, loss of radiance of the complexion or dull complexion, loss of suppleness of the skin and cracks.

The moisturizing effect of the composition used according to the invention can in particular be measured by corneometry, according to usual techniques well known to those skilled in the art.

Preferably, the carob pulp extract used according to the invention, or the composition used in the process according to the invention, are applied to dry, preferably non-pathological skin. They can be advantageously applied to the skin of the face, neck and possibly décolleté or alternatively on any part of the body. The composition containing this extract may be applied in the morning and / or in the evening, over the entire face, the neck and possibly the décolleté or even the body. The composition used according to the invention generally comprises, in addition to the extract described above, a physiologically acceptable and preferably cosmetically acceptable medium, that is to say which is suitable for use in contact with human skin without risk. toxicity, incompatibility, instability, allergic response and in particular that does not cause feelings of discomfort (redness, tightness, tingling ...) unacceptable to the user.

This medium generally contains water and possibly other solvents such as ethanol. The composition used according to the invention may be in any form suitable for topical application to the skin and in particular in the form of an oil-in-water, water-in-oil or multiple emulsion (E / H / E or H / E / H), which may optionally be microemulsions or nanoemulsions, or in the form of hydrodispersion, solution, aqueous gel or powder. It is preferred that this composition is in the form of an oil-in-water emulsion. This composition is preferably used as a care or cleansing product for the skin of the face and / or the body and may be in the form of a fluid, a gel or a mousse, for example packaged in a bottle. pump, aerosol or tube, or cream packaged for example in a pot. Alternatively, it may be in the form of a makeup product and in particular a foundation or loose or compact powder. It may contain various adjuvants, such as at least one compound chosen from: oils, which may be chosen in particular from: linear or cyclic, volatile or nonvolatile silicone oils, such as polydimethylsiloxanes (dimethicones) polyalkylcyclosiloxanes (cyclomethicones) and polyalkylphenylsiloxanes (phenyldimethicones); synthetic oils such as fluorinated oils, alkyl benzoates and branched hydrocarbons such as polyisobutylene; vegetable oils and especially soy or jojoba; and mineral oils such as paraffin oil; Waxes, such as ozokerite, polyethylene wax, beeswax or carnauba wax; the silicone elastomers obtained especially by reaction, in the presence of a catalyst, of a polysiloxane having at least one reactive group (hydrogen or vinyl, in particular) and bearing at least one alkyl group (especially methyl) or phenyl, terminal and or lateral, with an organosilicone such as an organohydrogen polysiloxane; Surfactants, preferably emulsifiers, whether they are nonionic, anionic, cationic or amphoteric, and in particular esters of fatty acids and of polyols such as esters of fatty acids and of glycerol, esters of fatty acids and sorbitan, fatty acid and polyethylene glycol esters and esters of fatty acids and sucrose; ethers of fatty alcohols and polyethylene glycol; alkylpolyglucosides; polyether modified polysiloxanes; betaine and its derivatives; polyquaterniums; sulfate salts of ethoxylated fatty alcohols; sulfosuccinates; sarcosinates; alkyl and dialkyl phosphates and their salts; and fatty acid soaps; co-surfactants such as linear fatty alcohols and in particular cetyl and stearyl alcohols; thickeners and / or gelling agents, and in particular homopolymers or copolymers, crosslinked or otherwise, hydrophilic or amphiphilic, of acryloylmethylpropanesulphonic acid (AMPS) and / or of acrylamide and / or acrylic acid and / or salts or esters of acrylic acid; xanthan or guar gum; cellulosic derivatives; and silicone gums (dimethiconol); organic screening agents, such as dibenzoylmethane derivatives (including butyl methoxydibenzoylmethane), cinnamic acid derivatives (including ethylhexyl methoxycinnamate), salicylates, para-aminobenzoic acids, (3- (3 ') diphénylacrylates, benzophenones, benzylidene camphor derivatives, phenylbenzimidazoles, triazines, phenylbenzotriazoles and anthranilic derivatives; inorganic filters, based on inorganic oxides in the form of pigments or nanopigments, coated or uncoated, and in particular based on titanium dioxide or zinc oxide, dyes, preservatives, fillers, and in particular soft-focus powders, which can be chosen in particular from polyamides, silica, talc, mica, fibers (especially polyamide or cellulose), sequestering agents such as EDTA salts, perfumes, and mixtures thereof, without this list being limiting.

Examples of such adjuvants are cited in particular in the CTFA (International Cosmetic Ingredient Dictionary and Handbook published by The 2905857 12 Cosmetic, Toiletry and Fragrance Association, Seme Edition, 2002) which describes a wide variety, without limitation, of cosmetic ingredients and pharmaceuticals customarily used in the skin care industry, which are suitable for use as additional ingredients in the compositions of the present invention. The composition used according to the invention may further comprise other active agents than the locust bean pulp extract and in particular at least one active agent chosen from: keratolytic agents and in particular α-hydroxy acids such as glycolic acids, lactic and citric, and their esters or salts; R-hydroxy acids, such as salicylic acid and its derivatives including n-octanoyl-s-salicylic acid; agents which increase the differentiation and / or corneification of keratinocytes, either directly or indirectly by stimulating for example the production of R-endorphins, such as extracts of Thermus thermophilus or shells of Theobroma cocoa beans, water-soluble extracts of corn, peptide extracts of Voandzeia substerranea and niacinamide; epidermal lipids and epidermal lipid synthesis enhancing agents, either directly or by stimulating certain glucosidases which modulate the deglycosylation of lipid precursors such as glucosylceramide to ceramides, such as phospholipids, ceramides, lupine protein hydrolysates; and dihydrojasmonic acid derivatives; humectants, such as polyols and in particular glycerol, glycosaminoglycans such as hyaluronic acid, sugars and their alkyl esters, amino acids such as glycine, arginine, histidine, alanine , threonine, lysine, glutamic acid, taurine, proline, serine and their derivatives, pyrrolidone 5 carboxylic acid (PCA) and its salts, urea and its derivatives, ectoin, glucosamine , creatine, choline, betaine, inorganic salts such as the salts of chlorine, sodium, potassium, calcium, magnesium, zinc, manganese, phosphate and synthetic humectant polymers such as homoet copolymers of methacryloyloxyethyl phosphorylcholine and homo- and copolymers of glyceryl (meth) acrylate; filling systems; percutaneous absorption-promoting agents such as alcohols, fatty alcohols and fatty acids and their ester or ether derivatives, pyrrolidones, terpenes, essential oils and α-hydroxy acids; anti-oxidant and / or antiradical and / or anti-pollution agents, such as tocopherol and its esters, ascorbic acid and its alkyl- and phosphoryl esters and certain extracts of plants or algae and in particular Thermus thermophilus ; and their mixtures, without this list being limiting. The aforementioned agents may optionally be vectorized in targeting systems such as liposomes, micelles such as micelles based on sodium lactate and sodium PCA marketed by the Serobiological Laboratories under the trade name Micelles Sèches LS8695, oleosomes, nanocapsules or nanoparticles, or be conveyed in polymeric matrices such as the film-forming matrix based on gum acacia and alginate carrying serine, available from the company COLETICA under the trade name MICROPATCH. By "filling systems" is meant dermal delivery systems for compounds capable of absorbing the water contained in the skin and increasing in volume as a result of this absorption. Examples of such systems are especially filling spheres based on glycosaminoglycans such as spheres based on hyaluronic acid or chondroitin sulfate sold especially by the company COLETICA. It has been found by the Applicant that the combination of the extract of carob pulp with one or more of the active agents described above makes it possible to advantageously combine, in the same formula, the long-term and immediate effects of these two types of carbohydrate pulp. and thus achieve maximum hydration and long-lasting skin.

The invention therefore also relates to a cosmetic composition comprising, in a physiologically acceptable medium, a carob pulp extract and at least one active agent chosen from: keratolytic agents, agents increasing the differentiation and / or corneification of keratinocytes, epidermal lipids and agents for increasing epidermal lipid synthesis, humectants, filling systems and mixtures thereof.

More particularly, the invention relates to a composition comprising, in a physiologically acceptable medium, an extract of locust bean pulp and at least one active ingredient selected from a fermented extract of Thermus thermophilus, sodium hyaluronate and mixtures thereof. The invention will now be illustrated by the following non-limiting examples. EXAMPLES Example 1: Preparation of Locust Bean Pulp Extract A carob pulp extract was prepared according to the following steps: 1 - Extraction Ethanol 92 250 kg of dried carob pods freed from their seeds were ground. The crushed material is charged to a continuous reactor of 2000 liters. The extraction solvent, consisting of 92 ethanol, is charged to the reactor and the mixture is heated to 60 ° C. 7500 liters of solvent are circulated for 30 hours in the reactor, a solvent / material ratio of 30.degree. / 1. The solvent is then evaporated under vacuum.

125 kg of carob oleoresin are obtained. The yield of this step is 50%. 2 - Decolorization of the oleoresin Two hot washes of the oleoresin are carried out with 96.2 ethanol and activated carbon: 2905857 16 ter wash: 1000 g of oleoresin are mixed with 5000 ml of ethanol 96.2 and 125 g of charcoal. Strongly stirred for 2 hours at 50-60 ° C and then allowed to stand at room temperature for 2 hours. After filtration of the Buchner solution, the primary filtrate is recovered. 2nd wash: the whole of the primary filtrate is taken up again. 500 ml of ethanol is added to 96.2 and 125 g of active charcoal. Stirred for two hours at 50-60 ° C and then allowed to stand at room temperature for 12 hours. After filtration of the Buchner solution, the final filtrate is recovered.

This filtrate is then filtered again on a conical filter in order to remove the last residues of activated charcoal, and then the ethanol is evaporated off using a rotary evaporator under vacuum.

The yield of this discoloration operation is 60%. The total yield of the process is 30%.

Example 2: Test for stimulation of the expression of the MT / SP1 matriptase mRNA (RT-PCR) The activity of the extract obtained in Example 1 was evaluated and then diluted to 70% by weight in propylene glycol on the expression of MT / SP1 matriptase mRNA. The effect of this extract was evaluated by real-time polymerase chain reaction (RT-PCR) in order to quantify the expression of the messenger RNA. Matriptase 5 MT / SP1 in a treated sample versus an untreated sample. The results are normalized with respect to the expression of housekeeping genes in these samples.

The results are expressed as a number of times of increase or decrease in expression of the target gene (MT / SP1 matriptase) in the treated sample. The cDNA / mRNA sequences of the investigated genes were obtained from GenBank. Target Gene: Matriptase MT / SP1 Domestic Gene: (3-2-microglobulin All PCR primers were obtained using PRIMER3 software from Whitehead Institute for Biomedical Research.The mRNA was isolated using the Qiagen RNeasy kit ( QIAGEN) according to the manufacturer's recommendations The reverse transcription in cDNA was carried out using the gene Amp RNA PCR kit (Applied Biosystems) according to the manufacturer's recommendations The real-time PCR measurement was carried out using the iCYCLER IQ (Biorad) apparatus with SYBR Green I detection In all assays, the cDNA was amplified using a standardized program, each sample was loaded with IQ SYBR Green I supermix, water and the primer (stock), the final amount of cDNA by reaction corresponded to 50 ng of total RNA used for reverse transcription The relative quantification of target gene expression was performed using the mod Mathematical Pfaffl (Pfaffl, MW, Nucleic Acids Res. 29 (9), p. E45, 2001). The test was performed on normal human keratinocytes cultured in triplicate with the extract for six hours. The results obtained were confirmed using cells from two different donors.

Results: The carob pulp extract tested at 0.10 stimulated the expression of the matriptase mRNA by 86% (standard deviation: 0.05), compared with the untreated control. Example 3: Stimulation test for the expression of the MT / SP1 matriptase protein (immunofluorescence) Protocol: The stimulation of the expression of the matriptase obtained with the extract described in Example 1 was evaluated in a model of reconstructed skin. This model was prepared in the following manner: a collagen solution containing rat tail type I collagen (BD), DMEM 10X medium (Gibco), sodium bicarbonate (Gibco) and fibroblasts was poured into 24 mm cell culture inserts (Falcon, Becton Dickinson, Schwechat, Austria) which were placed in six-well plates (Falcon). After two hours at 37 ° C., the gels were equilibrated in KGM at 37 ° C. in a 5% CO2 / 95% air environment, in a humidified incubator. After two hours, KGM containing keratinocytes was added to the gel. After immersion of the culture overnight, the medium was replaced with serum-free keratinocyte medium (SKDM, which is a Cal'-rich medium consisting of KGM without bovine pituitary extract, SIGMA transferrin, SIGMA BSA and SIGMA L-ascorbic acid) outside the insert, and the keratinocytes were maintained at the air-liquid interface. The culture medium of the reconstructed skins was replaced every other day by preheated fresh SKDM, and the culture was continued for up to seven days, with or without the tested extract. The reconstructed skin was then prepared for their immunofluorescence analysis. Sections 5 μm thick were made from reconstructed skins fixed with paraformaldehyde and then frozen. Nonspecific binding of the sections were blocked with serum (bovine serum albumin). The reconstructed skin samples thus prepared were incubated with anti-matriptase antibody (Bethyl Laboratories, TX) and then labeled in a second step with a second antibody complexed to a fluorescent agent (anti-rabbit Alexa Fluor 546, Molecular Probes, UK). ). Detection was by immunofluorescence. The slides were examined using a Leica microscope.

Results: Locust bean pulp extract at 0.1% (w / w) was visibly stimulated to express MT / SP1 matriptase at the level of stratum granulosum. These results were confirmed using cells from two donors. Example 4: Cosmetic Compositions The following compositions can be prepared in quantities conventional to those skilled in the art. below are expressed as percentages by weight. The ingredients in capital letters are identified according to the name I: NCI.

15 3A - Cream gel (oil-in-water emulsion) Tetrasodium EDTA 0.05% Glycerine 5.00% 20 Water phase gelling 4.00% Alcohol 3.00% Preservatives 0.50% Locust bean pulp extract ('0.01% Sodium hyaluronate 3.00% 25 Cyclomethicone 8.00 % Dimethicone 3.00% Isononyl isononanoate 3.00% Perfume qs Colorants qs 30 Water qs 100.00% (1) as described in Example 1 and then diluted to 70% by weight in propylene glycol 2905857 21 This composition can be applied daily , morning and / or evening, on the skin of the face to moisturize it and make it supple, smooth and luminous.

3B - Serum (water-in-silicone emulsion) Glycerine 5.00% Preservatives qs 10 Alcohol 10.00% Carob pulp extract ('0.10% GLYCERYL POLYMETHACRYLATE & PROPYLENE GLYCOL (2) 10.00% Mixture of sugars and amino acids (3) 3.00% Sodium pyrrolidone carboxylate 4.00% Sodium hyaluronate 2.50% SACCHARIDE ISOMERATE (4 1.00% Moisture dry micelles (5) 20.00% Thermus thermophillus extract (6) 3.00% CYCLOPENTASILOXANE & PEG / PPG-18/8 DIMETHICONE 20.00% 20 Dyestuffs qs Water qs 100.00% (1) as described in Example 1 and then diluted to 70% by weight in propylene glycol (2) LUBRAJEL MS from GUARDIAN LABORATORIES (3) HYDRATYL LS 8453 from LABORATOIRES SEROBIOLOGIQUES 4> PENTAVITIN from PENTAPHARM (5) SERUMOLOGICAL LABORATORY LS8695 Dry Micelles (6) Venuceane from SEDERMA This composition can be applied daily, morning and / or evening, on particularly dehydrated skin and / or exposed to environmental aggressions. t, to improve their comfort and appearance.

3C - Cream (oil-in-water emulsion) 5 Panthenol 0.40% Carboxyvinyl polymer 0.60% 25% sodium hydroxide solution 0.30% Sodium hyaluronate 0.30% 10 Glycerin 3.00% Locust bean pulp extract ('0.05% GLYCERYL POLYMETHACRYLATE & PROPYLENE GLYCOL (2) 10.00% Glyceryl stearate 1.50% Cetyl alcohol 1.50% Polyoxyethylene (40 EO) stearate 2.00% Oxyethylenated stearyl alcohol (2 EO) 0.50% Octyldodecyl neopentanoate 5.00% C12-C15 alkyl benzoate 3.00% Vegetable oils 3.00 % Phytosterol ester 1.00% Tocopheryl acetate 1.00% Silicone oils 4.00% Disodium EDTA 0.05% Preservatives 0.90% Colorants qs Water qs 100.00% 01) As described in Example 1 and then diluted to 70% by weight in propylene glycol 30 (2) LUBRAJEL MS from GUARDIAN LABORATORIES This cream can be applied to dry skin in the morning and / or evening, to improve its softness and

Claims (15)

  A cosmetic process for the care of human skin, for moisturizing and / or protecting it from drying, comprising topically applying to the skin a composition containing a carob pulp extract ( ceratonia siliqua).   2. Method according to claim 1, characterized in that the extract is derived from seeds of carob seeded.   3. Method according to claim 1 or 2, characterized in that the extract is obtainable by alcohol extraction with the aid of a monoalcohol and / or a glycol, optionally mixed with alcohol. 'water.   4. Process according to claim 3, characterized in that the extraction solvent comprises ethanol.   5. Method according to any one of claims 1 to 4, characterized in that it is used to fight against cutaneous signs resulting from a disturbed barrier function.   6. Method according to claim 5, characterized in that said signs are chosen from: roughness of the skin, redness, tightness, tingling, itching, loss of radiance of the complexion, loss of flexibility of skin and chapped skin 2905857 25   7. Method according to any one of claims 1 to 6, characterized in that it is carried out on a dry skin.   8. Cosmetic use of a carob pulp extract (ceratonia siliqua) to moisturize and / or protect human skin from drying.   9. Use according to claim 8, characterized in that the extract stimulates the expression of the MT / SP1 matriptase in the epidermis.   10. A cosmetic composition comprising, in a physiologically acceptable medium, a carob pulp extract (ceratonia siliqua) and at least one active agent chosen from keratolytic agents, agents increasing the differentiation and / or corneification of keratinocytes, epidermal lipids and agents increasing the synthesis of epidermal lipids, humectants, agents facilitating percutaneous absorption, filling systems and mixtures thereof.   11. Composition according to claim 10, characterized in that the active agent is selected from a fermented extract of Thermus thermophilus, sodium hyaluronate and mixtures thereof.   12. Carob extract (ceratonia siliqua), characterized in that the extract is obtainable by extraction of the carob pulp with at least one alcoholic solvent. 2905857 26   13. Extract according to claim 12, characterized in that the extract is derived from seeded carob pods. 5   14. Extract according to claim 13, characterized in that the extract is obtainable by alcohol extraction with a mono-alcohol and / or glycol, optionally mixed with water. 10   15. Extract according to claim 14, characterized in that the extraction solvent comprises ethanol.