FR2811682A3 - Use of labeled dextran for in vitro assessment of increased cell permeability, cell death and apoptosis - Google Patents

Use of labeled dextran for in vitro assessment of increased cell permeability, cell death and apoptosis

Info

Publication number
FR2811682A3
FR2811682A3 FR0009235A FR0009235A FR2811682A3 FR 2811682 A3 FR2811682 A3 FR 2811682A3 FR 0009235 A FR0009235 A FR 0009235A FR 0009235 A FR0009235 A FR 0009235A FR 2811682 A3 FR2811682 A3 FR 2811682A3
Authority
FR
France
Prior art keywords
dextran
cell
apoptosis
labeled dextran
cell death
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR0009235A
Other languages
French (fr)
Other versions
FR2811682B3 (en
Inventor
Mohamed Moumaris
Nisen Abuaf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Assistance Publique Hopitaux de Paris APHP
Original Assignee
Assistance Publique Hopitaux de Paris APHP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Assistance Publique Hopitaux de Paris APHP filed Critical Assistance Publique Hopitaux de Paris APHP
Priority to FR0009235A priority Critical patent/FR2811682B3/en
Publication of FR2811682A3 publication Critical patent/FR2811682A3/en
Application granted granted Critical
Publication of FR2811682B3 publication Critical patent/FR2811682B3/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2510/00—Detection of programmed cell death, i.e. apoptosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Utilisation du dextran marqu e pour la d etection de la perm eabilisation de la membrane plasmique ou la mort cellulaire. L'invention concerne le dextran, mol ecule disponible dans des poids mol eculaires diff erents, permettant d'analyser le degr e de la perm eabilit e cellulaire. Le dextran coupl e à un fluorochrome, enzymomarqu e ou radiomarqu e est utilis e pour le diagnostic et la d etection des cellules mortes. Le dextran marqu e est particulièrement destin e à suivre l' evolution des diff erents stades de l'apoptose et l' etendue des cellules en n ecrose; rencontr es dans divers situations comme l'inflammation, le cancer, les maladies infectieuses, le d eveloppement embryonnaire, la r egulation du système immunitaire et nerveux, la toxicit e cellulaire, le renouvellement tissulaire physiologique et le vieillissement cellulaire. Dans les techniques in-vitro le dextran marqu e permet d' evaluer le pourcentage de cellules mortes.Use of labeled dextran for the detection of plasma membrane permeabilization or cell death. The invention relates to dextran, a molecule available in different molecular weights, for analyzing the degree of cell permeability. Dextran coupled to a fluorochrome, enzyme brand or radiolabel is used for the diagnosis and detection of dead cells. The marked dextran is particularly intended to follow the evolution of the different stages of apoptosis and the extent of necrotic cells; encountered in various situations such as inflammation, cancer, infectious diseases, embryonic development, regulation of the immune and nervous system, cell toxicity, physiological tissue renewal and cell aging. In in-vitro techniques, labeled dextran makes it possible to evaluate the percentage of dead cells.

FR0009235A 2000-07-13 2000-07-13 USE OF THE DEXTRAN MARKED FOR THE DETECTION OF THE PERMEABILIZATION OF THE PLASMIC MEMBRANE OR CELL DEATH Expired - Fee Related FR2811682B3 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR0009235A FR2811682B3 (en) 2000-07-13 2000-07-13 USE OF THE DEXTRAN MARKED FOR THE DETECTION OF THE PERMEABILIZATION OF THE PLASMIC MEMBRANE OR CELL DEATH

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0009235A FR2811682B3 (en) 2000-07-13 2000-07-13 USE OF THE DEXTRAN MARKED FOR THE DETECTION OF THE PERMEABILIZATION OF THE PLASMIC MEMBRANE OR CELL DEATH

Publications (2)

Publication Number Publication Date
FR2811682A3 true FR2811682A3 (en) 2002-01-18
FR2811682B3 FR2811682B3 (en) 2003-04-11

Family

ID=8852497

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0009235A Expired - Fee Related FR2811682B3 (en) 2000-07-13 2000-07-13 USE OF THE DEXTRAN MARKED FOR THE DETECTION OF THE PERMEABILIZATION OF THE PLASMIC MEMBRANE OR CELL DEATH

Country Status (1)

Country Link
FR (1) FR2811682B3 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010073014A1 (en) * 2008-12-24 2010-07-01 Immunosolv Limited Cell separation technique
US9804153B2 (en) 2012-08-29 2017-10-31 Inguran, Llc Magnetic removal or identification of damaged or compromised cells or cellular structures
US10379026B2 (en) 2012-08-29 2019-08-13 Inguran, Llc Cell processing using magnetic particles

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010073014A1 (en) * 2008-12-24 2010-07-01 Immunosolv Limited Cell separation technique
CN102333860A (en) * 2008-12-24 2012-01-25 易优诺生物科技有限责任公司 Cell separation technique
US9804153B2 (en) 2012-08-29 2017-10-31 Inguran, Llc Magnetic removal or identification of damaged or compromised cells or cellular structures
US10324086B2 (en) 2012-08-29 2019-06-18 Inguran, Llc Magnetic removal or identification of damaged or compromised cells or cellular structures
US10379026B2 (en) 2012-08-29 2019-08-13 Inguran, Llc Cell processing using magnetic particles
US11531026B2 (en) 2012-08-29 2022-12-20 Inguran, Llc Magnetic removal or identification of damaged or compromised cells or cellular structures
US11630108B2 (en) 2012-08-29 2023-04-18 Inguran, Llc Cell processing using magnetic particles
US11662344B2 (en) 2012-08-29 2023-05-30 Inguran, Llc Magnetic removal or identification of damaged or compromised cells or cellular structures
US12332239B2 (en) 2012-08-29 2025-06-17 Inguran, Llc Magnetic removal or identification of damaged or compromised cells or cellular structures

Also Published As

Publication number Publication date
FR2811682B3 (en) 2003-04-11

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