FR2780286A1 - Novel compositions useful as non-transfer cosmetics and dermo-pharmaceuticals - Google Patents

Abstract

Topical compositions comprising at least two antibodies, at least one of which recognizes body surface epitopes and the other may recognize these or colors or active material epitopes, these being optionally coupled with colors and cosmetic or dermatological active materials, are new.- DETAILED DESCRIPTION - The new compositions comprise a cosmetic, dermatological , hygiene or pharmaceutical medium comprising: - (1) at least one antibody (I) that has a protein domain capable of recognizing the epitope of skin, nails, lips and eyelashes, and is optionally coupled with a pigment, dye, cosmetic or dermatological active material; and - (2) at least one second antibody (II) that comprises the following: - (a) a protein domain capable of recognizing an epitope of (I); and/or - (b) a protein domain capable of recognizing the epitope of a pigment, dye, cosmetic or dermatological active ingredient, being optionally linked to a pigment, dye, or active component.- INDEPENDENT CLAIMS are also included for obtaining (I) and/or (II) by immunizing a mammal with the epitope of a pigment, dye, or active material, then taking a sample of the blood of the treated animal and isolating the antibody. Alternatively, (I) and/or (II) are obtained by taking the liver cells from the animal and fusing them with immortalized cells. The antibodies synthesized by the hybridomas are collected.- ACTIVITY - Antibacterial; antiviral; antifungal; anti-inflammatory; anesthetic; antiacne; antiseptic; antidandruff.- MECHANISM OF ACTION - None given

Description

New antibodies, topical compositions containing these

  The present invention relates to novel antibodies, to compositions for topical application, in particular cosmetic, dermatological, hygienic or pharmaceutical, containing these antibodies and to the use of these. -this in or for the manufacture of compositions for topical application. More specifically, the invention relates to a cosmetic, dermatological, hygienic or pharmaceutical composition without transfer for the care and / or make-up of the skin both of the face and of the human body, of the mucous membranes such as the lips and the inside of the lower eyelids, or integuments like

  eyelashes, eyebrows, nails or hair.

  This composition can be presented in particular in the form of a product cast in a stick or in a cup such as lipsticks or lip balms, cast foundations, concealer products, eyeshadows or blushes, in the form of paste or more or less fluid cream such as foundations or fluid lipsticks, eye liners, sun protection compositions or hair color

  skin, mascaras, dermatological creams.

  Makeup or skin care or lip care products for human beings such as foundations or lipsticks generally contain fatty phases such as waxes and oils, pigments and / or fillers, and possibly additives. as cosmetic or dermatological active ingredients. They may also contain so-called "pasty" products, of flexible consistency, making it possible to obtain pasta, colored or not, to be applied with a brush. These compositions, when applied to the skin or the lips, have the drawback of transferring, that is to say of depositing at least in part leaving traces, on certain supports with which they can be brought into contact. , and in particular a glass, a cup, a cigarette, a garment or the skin. This results in poor persistence of the applied film, requiring regular renewal of the application of the foundation or lipstick composition. In addition, the appearance of these unacceptable traces, particularly on blouse collars, may exclude certain women from

  the use of this type of makeup.

  For several years, cosmeticians have been interested in lipstick compositions and more recently in

  "no transfer" foundation compositions. Document JP-A-62-

  61911 describes compositions of lipstick, of eyeliner, of "no transfer" foundation foundation comprising one or more volatile silicones associated with one or more hydrocarbon waxes. These compositions, although having improved "no transfer" properties, have the drawback of leaving a film on the lips, after evaporation of the silicone oils, which becomes uncomfortable

  over time (feeling of dryness and tightness).

  dismissing a number of women from this type of lipstick.

  The documents EP-A-497144 and FR-A-2 357 244 describe so-called "transfer-free" compositions, which have the drawback of being uncomfortable, of having any cosmetic properties and of being difficult to formulate. In addition,

  "no transfer" properties of these compositions are very average.

  There therefore remains the need for a composition which does not have the above drawbacks, and which in particular has total "transfer-free" properties, even at pronounced pressure, a more or less shiny appearance, adapted to the desire for consumer, does not dry out over time the skin or the lips on which

it is applied.

  The Applicant has found, quite surprisingly, that the use of antibodies coupled to a pigment and / or a dye and / or a cosmetic or dermatological active ingredient in a cosmetic, dermatological, pharmaceutical or hygienic composition could make it possible to obtain a composition which, after application, exhibits very good hold, does not transfer at all, resists water, while being very pleasant to apply and to wear throughout the day. Patent EP 570 583 has already envisaged the use of uncoupled anti-keratin antibodies in a completely different field, in particular for styling shampoos or foams in order to condition the

hair.

  The present invention therefore relates to new antibodies coupled to a pigment and / or a dye and / or an active

cosmetic or dermatological.

  Another subject of the invention is a process for obtaining

such antibodies.

  An object of the invention is also a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium, and in addition, these

new antibodies.

  According to the invention, it is possible to use any dosage form for the composition. Thus, it is possible to introduce into the composition any ingredient facilitating the qualities of spreading and adhesion on the skin, the semi-mucous membranes or the mucous membranes, particularly advantageous, as well as a smooth and pleasant touch. These compositions also have the advantage of removing make-up with a conventional cleansing milk. This is quite remarkable since the compositions of the prior art with high "no transfer" properties are generally sold with a specific make-up removing product, which introduces an additional constraint for

the user.

  It has also been found, quite surprisingly, that when using antibodies coupled to cosmetic active agents or

  dermatological, the action of said active was reinforced.

  In addition, it is now possible to use in make-up or care compositions, pigments, dyes or cosmetic or dermatological active agents which, until now, could not be, the penetration of these compounds into the dermis being able to cause irritation. Indeed, the coupling of such pigments, dyes or cosmetic or dermatological active agents with the antibodies of the present invention makes it possible to ensure the maintenance of said pigments, dyes or cosmetic or dermatological active agents in

surface of the dermis.

  Other objects of the invention are therefore the uses in or for the manufacture of a composition for topical application of these

new antibodies.

  The subject of the invention is also a method of cosmetic care or of making up the lips, the skin or the hair consisting in applying respectively to the lips, the skin, including

  including the scalp, or hair, such a composition.

  The antibodies of the present invention, comprising a protein domain allowing the recognition of an antigen, are essentially characterized in that said antibodies are coupled to a pigment and / or a dye and / or a cosmetic or dermatological active ingredient. Within the meaning of the present invention, the antibodies or immunoglobulins are in particular those synthesized by B lymphocyte cells in mammals, such as for example in

  rabbit, rat, mouse, hamster or man.

  The protein domain allowing the recognition of an antigen can in particular correspond to an immunoglobulin fragment containing the protein domain necessary for the

fixation of the antigen.

  This protein domain can, for example, correspond to an “antigen binding” (Fab) fragment, which carries the antibody activity of the

immunoglobulin molecule.

  The antigen recognized by this protein domain can in particular correspond to an epitope of the skin and / or the nails and / or the lips.

and / or eyelashes and / or hair.

  Preferably, this antigen is an epitope of keratin.

  In another embodiment of the invention, said antigen is an epitope of a second antibody comprising a protein domain allowing the recognition of an epitope of the

  skin and / or nails and / or lips and / or eyelashes and / or hair.

  The antibodies can in particular be coupled to pigments chosen in particular from mineral or organic pigments

  can be white, colored or pearlescent.

  Among the mineral pigments, mention may be made of titanium dioxide, optionally surface-treated, zirconium or cerium oxides, as well as iron or chromium oxides, violet

  manganese, ultramarine blue, chromium hydrate and ferric blue.

  Among the organic pigments, mention may be made of carbon black, pigments of the D & C type, and lacquers based on cochineal carmine,

  barium, strontium, calcium, aluminum.

  The pearlescent pigments can be chosen from white pearlescent pigments such as mica coated with titanium or bismuth oxychloride, colored pearlescent pigments such as mica titanium with iron oxides, mica titanium with especially ferric blue or chromium oxide, titanium mica with an organic pigment of the type

  cited above as well as pearlescent pigments based on bismuth oxychloride.

  The antibodies of the present invention can be coupled to dyes notably chosen from paraphenylenediamines, paraaminophenols, orthophenylenediarmines, heterocyclic bases such as for example pyridine derivatives, pyrimidine derivatives, pyrazole derivatives and derivatives

  pyrazolopyrimidiniques and liposoluble dyes.

  The liposoluble dyes are for example Sudan red, DC Red 17, DC Green 6, P-carotene, soybean oil, Sudan brown, DC Yellow, 11, DC purple 2, DC orange 5 or quinoline yellow. The antibodies can also be coupled to a cosmetic or dermatological active agent, in particular chosen from the following cosmetic or dermatological actits: - agents modulating differentiation and / or proliferation and / or skin pigmentation such as retinoic acid and its isomers, retinol and its esters, retinoids in particular those described

  in documents FR-A-2 570 377, EP-A-199636, EP-A-325540, EP-

  A-402072, retinal, vitanmine D and its derivatives, estrogens such as estradiol, kojic acid or hydroquinone; - antibacterials such as clindamycin phosphate, erythromycin or antibiotics of the tetracycline class; - antiparasitics, in particular metronidazole, crotamiton or pyrethroids; - antifungals, in particular compounds belonging to the class of imidazoles such as econazole, ketoconazole or miconazole or their salts, polyene compounds, such as amphotericin B, compounds of the allylamine family, such as terbinafine, or octopirox; - steroidal anti-inflammatory agents, such as hydrocortisone, betamethasone valerate or clobetasol propionate, or non-steroidal anti-inflammatory agents such as ibuprofen and its salts, diclofenac and its salts, acetylsalicylic acid, acetaminophen or glycyrrhetinic acid; - anesthetic agents such as lidocaine hydrochloride and its derivatives; - antipruritic agents such as thenaldine, trimeprazine or cyproheptadine; - antiviral agents such as acyclovir;

  - keratolytic agents such as alpha- and beta-acids

  hydroxy-carboxylic or beta-ketocarboxylic, their salts, amides or esters and more particularly alpha-hydroxy acids such as glycolic acid, lactic acid, tartaric acid, malic acid, citric acid, mandelic acid and in general fruit acids and beta-hydroxy acids such as salicylic acid and its derivatives, in particular alkoxylated derivatives such as n-octanoyl-5-salicylic acid; - anti-free radical agents, such as alpha-tocopherol or its esters, superoxide dismutases, certain metal chelators or ascorbic acid and its esters; - antiseborrheics such as progesterone; - anti-dandruff agents such as octopirox or zinc pyrithione; - anti-acne drugs such as retinoic acid or benzoyl peroxide; - antimetabolites; - anti-hair loss agents such as minoxidil; antiseptics such as triclosan or chlorhexidine digluonate; - organic or mineral filters such as octylmethoxycinammate, benzophenone, octocrylene, nanotitane or dibenzoylmethane derivatives; - restructuring of the stratum corneum such as ceramides or proteins; - moisturizers such as glycerin, urea or sorbitol; - nourishing active ingredients such as sugars, fatty acids

  essential or essential oils.

  The process for obtaining antibodies as defined above essentially consists in obtaining antibodies comprising a protein domain allowing the recognition of an antigen, in purifying said antibodies thus obtained. then coupling these antibodies with a pigment and / or a dye and / or a cosmetic active ingredient or

  to isolate the antibodies thus coupled.

  Antibodies comprising a protein domain allowing the recognition of an antigen can be obtained in a conventional manner, by immunizing a mammal with said antigen and then by drawing the blood of said immunized mammal. The antibodies obtained are

polyclonal.

  In a variant of the process of the invention, the antibodies comprising a protein domain allowing the recognition of an antigen are obtained by immunizing a mammal with said antigen, by taking cells from the spleen of said mammal, by fusing the latter with cells immortalized and

  collecting the antibodies synthesized by the hybridomas thus obtained.

These antibodies are monoclonal.

  It is also possible to use commercial antibodies,

  notably by the companies Pharmingen, Tebu or Calbiotech.

  The antibodies obtained as mentioned above can then be purified. The methods of purification of these antibodies are those conventionally used for the purification of antibodies such as precipitation with ammonium sulphate followed by exclusion chromatography, affinity chromatography on protein A-sepharose, chromatography of protein G affinity- sepharose, immunoglobulin light chain affinity chromatography (kappa chain) -sepharose or ion exchange chromatography (e.g. DE 52 chromatography

  marketed by the company Whatman).

  Antibodies can then be coupled with a pigment

  and / or a dye and / or a cosmetic or dermatological active ingredient.

  To do this, it is possible to use the usual antibody labeling techniques. These techniques use an attachment buffer allowing the attachment of the marker. The fixing buffer can be chosen according to the pigment and / or dye and / or cosmetic or dermatological active to be fixed. This buffer can, for example, consist of a solution of 0.5 M boric acid and 0.2 M NaCl at pH 7.4 or a solution of 0.1 M NaHCO3 and 0.1 M NaCl at pH 8.4 or 0.2 M NaHCO3 solution and 0.15

M NaCl at pH 9.0.

  The purified antibodies are then dialyzed against the fixing buffer. The concentration of these antibodies is then determined, by

example by optical density.

  Depending on this concentration, a more or less concentrated solution of pigments and / or dyes and / or cosmetic active ingredients

  or dermatological is then added.

  Said antibodies thus coupled to a pigment and / or a dye and / or a cosmetic or dermatological active agent can then be isolated by dialysis, for example in a solution of pH 7.4 of 0.1

  M of Tris-HCl, 0.1 M of NaN3 and 0.2 M of NaCl.

  These mixtures are then incubated for a variable duration

  at room temperature or in ice.

  It is then possible to determine the fixing capacity

  of these coupled antibodies, by performing an ELISA test.

  The present invention also relates to a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium, and in addition, at least

  an antibody as defined above.

  The present invention also relates to a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium, and in addition, at least one antibody coupled to a pigment and / or a dye and / or a cosmetic or dermatological active ingredient and comprising a protein domain allowing the recognition of an antigen which is an epitope of a second antibody comprising a protein domain allowing the recognition of an epitope of the skin and / or the nails and / or the lips and / or the eyelashes and / or hair and at least said second antibody. The compositions of the invention are in particular cosmetic, dermatological, hygienic or pharmaceutical compositions. They therefore contain compatible ingredients

  with the skin, mucous membranes and keratinous or dander fibers.

  The coupled antibodies of the present invention are present in the compositions in proportions of between 0.001 to 80%, preferably between 0.01 to 60% and more preferably

  between 1 to 50% by weight relative to the total weight of the composition.

  When a second antibody is present in the compositions of the invention, this second antibody can be present in proportions of between 0.001 to 80%, preferably between 0.01 to 60%, and more preferably between 1 to 50% by weight

  relative to the total weight of the composition.

  In the composition of the present invention, the cosmetic, dermatological, hygienic or pharmaceutical medium can comprise at least one fatty phase representing for example from 1 to

  99% of the total weight of the composition.

  The fatty phase can be chosen from waxes, oils, gums and / or pasty fatty substances, of vegetable, animal origin,

  mineral or synthetic, or silicone, and mixtures thereof.

  Among the waxes which may be present in the composition according to the invention, mention may be made of hydrocarbon waxes such as beeswax, Carnauba, Candellila, Ouricoury, Japanese wax, fiber waxes. cork or cane, paraffin waxes, lignite, microcrystalline waxes, lanolin wax, Montan wax, ozokerites, polyethylene waxes, waxes obtained by Fischer-Tropsch synthesis,

  hydrogenated oils, fatty esters and concrete glycerides at 25 C.

  It is also possible to use silicone waxes, among which mention may be made of alkyl, alkoxy and / or polymethylsiloxane esters. The waxes can be in the form of stable dispersions of colloidal particles of wax as they can be prepared according to known methods, such as those of "Microemulsions Theory and Practice", LM Prince Ed., Academic Press (1977), pages 21-32. As liquid wax at room temperature, there may be mentioned

Jojoba oil.

  Waxes can be present at 0-50% by weight

  in the composition and better from 10 to 30%.

  The fatty phase can be liquid. It can then consist of any oil that is cosmetically or dermatologically acceptable, and generally physiologically acceptable, in particular chosen from oils of mineral, animal, vegetable or synthetic origin, carbonaceous, hydrocarbon-based, fluorinated and / or silicone-based, alone or as a mixture in the extent to which they form a homogeneous and stable mixture and where they are compatible with

the intended use.

  "Liquid fatty phase" means any non-aqueous medium

liquid at room temperature.

  Mention may thus be made of hydrocarbon oils such as paraffin or petrolatum oil, hydrogenated polyisobutylene, mink, turtle, soybean oil, perhydrosqualene, sweet almond, calophyllum and palm oil. , grapeseed, sesame, corn, parlam, macaw, rapeseed, sunflower, cotton, apricot, castor, avocado, jojoba, olive or cereal sprouts ; esters of lanolic acid, oleic acid, lauric acid, stearic acid; fatty esters, such as isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, diisopropyl adipate, isononyl isononate, 2-ethyl palmitate hexyl, 2-hexyl-decyl laurate, 2-octyl-decyl palmrnitate, 2-octyl-dodecyl myristate or lactate, 2-diethyl-hexyl succinate, diisostearyl malate, glycerin triisostearate or diglycerin; higher fatty acids such as myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid or isostearic acid; higher fatty alcohols such as cetanol, stearyl alcohol or oleic alcohol, linoleic or linolenic alcohol, isostearic alcohol or octyl dodecanol; silicone oils such as polydimethylsiloxane (PDMS), optionally

  phenylates such as phenyltrimethicones, phenyltrimethylsi-

  loxydiphenylsiloxanes, diphenylmethyl-dimethyltrisiloxanes,

  diphenyldimethicones, phenyldimethicones, polymethyl-

  phenylsiloxanes, or optionally substituted by aliphatic and / or aromatic groups, optionally fluorinated or by functional groups such as hydroxyl, thiol and / or amine groups; polysiloxanes modified with fatty acids, fatty alcohols or polyoxalkylenes, fluorinated silicones, oils

perfluorinated.

  Among the preferred silicone oils, mention may be made of polydimethylsiloxanes, polymethylphenylsiloxanes, silicones comprising polyoxyalkylene sequences or grafts, in particular polyoxyethylene or copoly (oxyethylene-oxypropylene) such as dimethiconecopolyols, silicones carrying both hydrophobic hydrocarbon groups (for example C2-C30 alkyl groups) and polyoxyethylenated blocks or grafts or

  copoly (oxyethylenated / oxypropylenated) such as alkyldimethico-

  necopolyols, silicones carrying fluorinated or disturbed groups such as perfluoroalkylated polydimethylsiloxanes and

  alkylated perfluoro polymethylphenylsiloxanes.

  One or more volatile oils can also be used at room temperature. These volatile oils are favorable for obtaining a film with total "transfer-free" properties. After evaporation of these oils, a flexible film-forming deposit, not sticky on the skin or the mucous membranes, is obtained, depending respectively on the movement of the skin or the lips, to which the composition is applied. These volatile oils also facilitate the application of the

  composition on the skin, mucous membranes, integuments.

  These oils can be hydrocarbon oils, silicone oils optionally comprising alkyl or alkoxy groups

  at the end of the silicone or pendant chain.

  As volatile silicone oil which can be used in the invention, mention may be made of linear or cyclic silicones having from 2 to 7 silicon atoms, silicones optionally comprising alkyl or alkoxy groups having from 1 to 10 carbon atoms as well as isoparaffins in C8-C16. These volatile oils represent in particular

  from 30 to 97.99% of the total weight of the composition and better still from 30 to 75%.

  As volatile oil which can be used in the invention, it is possible to

  mention in particular octamethylcyclotetrasiloxane, decamethylcyclo-

  pentasiloxane, hexadecamethylcyclohexasilone, heptamethylhexyl-

  trisiloxane, heptamethylocytyltrisiloxane or C8- isoparaffins

  C16 such as "ISOPARs", PERMETYLs and in particular

  isodecane, isododecane or isohexadecane.

  Mention may also be made of organic solvents, alone or as a mixture, chosen from: - ketones which are liquid at room temperature such as

  methyl ethyl ketone, methyl isobutyl ketone, diisobutyl ketone.

  isophorone, cyclohexanone, acetone; - Alcohols liquid at room temperature such as ethanol, isopropanol, diacetone alcohol, 2-butoxyethanol, cyclohexanol; - glycols liquid at room temperature such as ethylene glycol, propylene glycol, pentylene glycol; - propylene glycol ethers which are liquid at room temperature such as propylene glycol monomethyl ether, propylene glycol monomethyl ether acetate, dipropylene glycol mono n-butyl ether; - short chain esters (having 3 to 8 carbon atoms in total) such as ethyl acetate, methyl acetate, propyl acetate, n-butyl acetate, acetate isopentyl; - ethers liquid at room temperature such as diethyl ether, dimethyl ether or diclorodiethyl ether; - alkanes liquid at room temperature such as decane, heptane, dodecane, cyclohexane; - aromatic cyclic compounds which are liquid at room temperature such as toluene and xylene; - aldehydes liquid at room temperature such as

benzaldehyde, acetaldehyde.

  These solvents are more particularly suitable for make-up and nail care: the composition then constitutes a nail varnish or a nail care product. As solvent we

  can also use water and hydroalcoholic media.

  As the non-aqueous medium, it is also possible to use those described.

in FR-A-2 710 646.

  The compositions according to the invention may also contain liposoluble and / or dispersible homopolymers and copolymers

in the fatty phase.

  Among these homopolymers and copolymers, mention may be made of polyolefins such as polyethylene, polybutene and polydecene; copolymers of (meth) acrylic esters and / or amides; copolymers of vinyl esters, for example ethylene / vinyl acetate copolymers; vinyl or (meth) acrylic homopolymers or copolymers carrying a silicone group such as for example graft copolymers with (meth) acrylic skeleton and macromer silicone grafts; copolymers with a skeleton or (meth) acrylic block and with grafts or hydrocarbon blocks, for example polyisobutylene; grafted or block copolymers with a backbone or polyorganosiloxane block and with (meth) acrylic and / or vinyl grafts or blocks; fluorinated or perfluorinated homopolymers or copolymbers, for example perfluorinated polyethers such as those marketed under the designation FOMBLIN, perfluorinated (meth) acrylic homo or copolymers, fluorinated vinyl homo or copolymers, fluorinated olefin homo or copolymers and

fluorinated poly (vinyl ether).

  The cosmetic, dermatological, hygienic or pharmaceutical medium can also contain one or more pearlescent pigments or

  no and / or one or more liposoluble dyes and / or fillers. The pigments can be white or colored, mineral and / or organic. We

  may include, among the mineral pigments, titanium dioxide, optionally surface-treated, zirconium or cerium oxides, as well as iron or chromium oxides, violet

  manganese, ultramarine blue, chromium hydrate and ferric blue.

  Among the organic pigments, mention may be made of carbon black,

  D & C type pigments, and cochineal carmine lacquers.

  barium, strontium, calcium, aluminum.

  The pearlescent pigments can be chosen from white pearlescent pigments such as mica coated with titanium or bismuth oxychloride, colored pearlescent pigments such as mica titanium with iron oxides, mica titanium with especially ferric blue or chromium oxide, titanium mica with an organic pigment of the type

  cited above as well as pearlescent pigments based on bismuth oxychloride.

  The composition of the invention can advantageously contain

charges.

  The fillers can be mineral or organic.

  lamellar or spherical. Mention may be made of talc, mica, silica,

  kaolin, nylon powders (Orgasol from Atochem), poly-f3-

  analine and polyethylene, Teflon, lauroyl-lysine, starch, boron nitride, powders of tetrafluoroethylene polymers, hollow microspheres such as Expancel (Nobel Industry), polytrap (Dow Corning) and microbeads of silicone resin (Toshiba's Tospearls, for example), calcium carbonate

  precipitate, carbonate and magnesium hydro-carbonate.

  hydroxyapatite, hollow silica microspheres (SILIcA BEADS from MAPRECOS), glass or ceramic nanocapsules, metal soaps derived from organic carboxylic acids having 8 to 22 carbon atoms, preferably 12 to 18 carbon atoms carbon, for example zinc magnesium or lithium stearate,

  zinc laurate or magnesium myristate.

  The fillers used, in particular the organic fillers of a polymeric nature, can be crosslinked or not and contain inside the particles one or more cosmetic, dermatological, hygienic or pharmaceutical active agents which can be released.

  after application of the composition.

  The pigments and the fillers may or may not be surface coated, in particular surface treated with silicones, amino acids, fluorinated derivatives or any other substance promoting the dispersion and compatibility of the pigment in the composition. The liposoluble dyes are for example Sudan red, DC Red 17, DC Green 6, [-carotene, soybean oil, Sudan brown, DC Yellow, 11, DC violet 2, DC orange 5 , quinoline yellow. They can represent from 0.01 to 20% of the weight of the

composition and better from 0.1 to 6%.

  The cosmetic, dermatological, hygienic or pharmaceutical medium can also contain at least one cosmetic or dermatological active ingredient. As cosmetic or dermatological active agents which can be used in the composition of the invention, mention may be made of active agents which can be coupled with the antibodies of the invention as defined above and in particular cosmetic oils, moisturizers, vitamins, essential fatty acids, sphingolipids, sunscreens, antioxidants, anti-acne, anti-inflammatories, tanning agents (in the absence of UV radiation), depigmenting agents, matting agents and their mixtures. Among the hydrating agents, mention may be made of glycerol and polymethacrylate

  glycerol, sodium hyaluronate and polyol or sugar esters.

  These active agents are used in the usual amount for those skilled in the art and in particular at concentrations of 0.001 to 20% of the total weight of the

composition.

  The composition according to the invention can, moreover, comprise, depending on the type of application envisaged, the constituents conventionally used in the fields under consideration, which are present in a

  amount appropriate to the desired dosage form.

  The composition may also comprise any additive usually used in such compositions, such as thickeners, antioxidants, perfumes, preservatives, surfactants, emulsifiers, liposoluble polymers such as polyalkylenes, in particular polybutene, polyacrylates and silicone polymers compatible with the fatty phase as well as polyvinylpyrolidone derivatives. Of course, those skilled in the art will take care to choose this or these optional additional compounds, and / or their quantity, in such a way that the advantageous properties of the

  composition according to the invention are not, or substantially not.

  altered by the proposed addition.

  Among the thickeners, there may be mentioned bentonites, treated silicas, guar gomnmes, block or graft polymers comprising at least one block soluble in the composition and one insoluble block, the (polyvinyl) pyrrolidone hexadiene,

  silicone gums and KSG silicones.

  In a particular embodiment of the invention, the compositions according to the invention can be prepared in the usual manner by a person skilled in the art. They can be in the form of a cast product and for example in the form of a stick or stick, or

  in the form of a cup usable by direct contact or with a sponge.

  In particular, they find an application as a poured foundation, blush or eyeshadow, lipstick, lip care base or balm, body makeup, concealer product. They can also be in the form of a flexible paste, of dynamic viscosity at 25 C of the order of I to 40 Pa.s or also of gel, of more or less fluid cream. They can then constitute foundations or lipsticks, sun or skin coloring products, treating shampoos, hair coloring products.

  hair, or dermatological products.

  The compositions of the invention can also be presented in particular in the form of an oily gel, an oily liquid or oil, a paste or a stick or also in the form of a vesicular dispersion containing ionic and / or nonionic liquids. These dosage forms are prepared according to the usual methods of the fields

considered.

  The compositions of the invention can also be presented

  as an oil-in-water or water-in-oil emulsion.

  The compositions for topical application may be in particular in the form of a product for caring for and / or making up the skin and / or the lips and / or the hair. Another object of the invention is the use in or for the manufacture of a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium of an antibody as defined above to reduce, or even suppress, the

  transfer of said composition for topical application.

  The present invention also relates to the use in or for the manufacture of a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium of an antibody as defined above coupled with a cosmetic or dermatological active ingredient such as defined above, to strengthen the action of said

  cosmetic or dermatological active.

  The invention also relates to the use in or for the manufacture of a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium of an antibody as defined above coupled with a pigment and / or a dye and / or cosmetic or dermatological active ingredient to prevent the penetration of said pigment and / or dye and / or cosmetic active ingredient or

dermatological in the dermis.

  The subject of the present invention is also a process for cosmetic care or for making up the lips, skin or hair of a human being consisting in applying to, respectively, the lips, the skin or

  hair, a cosmetic composition as defined above.

  Another object is a cosmetic care or make-up process for the lips, the skin or the hair, consisting in applying to the lips, the skin or the hair, respectively, a composition for topical application comprising a cosmetic, dermatological, hygienic medium. or pharmaceutical and, in addition, at least one antibody comprising a protein domain allowing the recognition of an epitope, respectively, of the lips, of the skin or of the hair, then of applying a second composition for topical application, comprising a cosmetic medium, dermatological, hygienic or pharmaceutical and, in addition, at least one antibody coupled to a pigment and / or a dye and / or a cosmetic or dermatological active agent recognizing the first

antibody applied.

  The invention is illustrated in more detail in the examples

following, nonlimiting examples.

Example 1:

  Obtaining coupled polyclonal and monoclonal antibodies: A. Obtaining polyclonal antibodies: Ground skin, lips, nails and hair are prepared and titrated in proteins. 2 ml of each of the ground materials, 0.25 to 0.5 mg / ml of PBS, are mixed with 2 ml of complete Freunds adjuvant (CFA, Sigma) or TiterMax or Ribi Adjuvant System (Ribi Imnmo

Chem).

  0.5 ml of this emulsion is injected into rabbits.

  14 days after this immunization, a little blood is taken in order to

determine the antibody titer.

  4 to 8 weeks after this first immunization, a reminder is

  performed under the same conditions.

  14 days after this reminder, a little blood is taken to determine the titer of the antibodies. When the title is sufficient, the

  rabbits are sacrificed and all of the blood is removed.

  B. Obtaining monoclonal antibodies: The immunization protocol is that described above for

obtain polyclonal antibodies.

The animal used is the mouse.

  The booster is given 10 to 14 days after the primary vaccination.

  Three days after the animals are sacrificed, the spleens removed and dissociated. Immortalized cells (SP2 / 0-Agl4, ATCC # CRL 1581) are fused with the spleen cells, the cells thus obtained are hybridomas. These are cloned by limiting dilution. The

  antibodies thus obtained are monoclonal antibodies.

  C. Purification of the Antibodies: The antibodies obtained, polyclonal or monoclonal, are then purified by precipitation with ammonium sulphate followed by exclusion chromatography or by affinity chromatography on protein A-sepharose or by affinity chromatography on protein G-sepharose, or by chain affinity chromatography

  kappa-sepharose and by ion exchange chromatography.

  D. Coupling of the purified antibodies with an organic lacquer.

  1 to 2 mg of purified antibodies are dialyzed against 500 ml of fixing buffer. After two days of dialysis, the antibody concentration is

  determined by reading optical density at 280 nM.

  gl of a 20 mg / ml solution of red organic lacquer are

added to the antibodies to be coupled.

  This mixture is incubated for 1 hour at a temperature of 20 C. Pigments not attached to the antibodies are removed by dialysis (0.1 M Tris-Cl, pH

7.4, 0.1% NaN3, 0.2 M NaCl).

  E. Determination of the capacity of the coupled antibodies:

  The capacity of these coupled antibodies is determined by an ELISA test.

  The crushed skin, nails, lips, hair are deposited on

a 96-well ELISA plate.

  The antibodies corresponding to the different ground materials are added to

  different concentrations and incubated for 2 hours at room temperature.

  The wells are then washed and the antibodies fixed on the plate.

  The number of antibodies fixed is evaluated by densitometric reading.

  It can be seen that the higher the D.O. (optical density), the higher the

  number of antibodies attached is important.

Example II:

  Compact face powder Anti-keratin antibody coupled to yellow iron oxides 1.6 g Anti-keratin antibody coupled to red iron oxides 1.7 g Anti-keratin antibody coupled to black iron oxides 1.5 g Methylparaben 0.3 g Perfume 0.2 g Magnesium carbonate 0.2 g Talc 73.8 g Titanium dioxide, alumina, glycerin and silica 3 g Nylon-12 10 g Dimethicone 2.51 g Dimethicone and trimethylsiloxysilicate 0.73 g Cethyl dimethicone 0.45 g Polymethylsilsesquioxane 5 g

Example III:

  Foundation Glyceryl stearate 2.9 g Glyceryl isostearate 2 g Mineral oil and lanolic alcohol 3 g Octyl palmitate 9 g Antikeratin antibody coupled to yellow iron oxides 1.45 g Anti-keratin antibody coupled to red iron oxides 0.67 g Anti-keratin antibody coupled to black iron oxides 0.25 g Anti-keratin antibody coupled to titanium dioxide 7.63 g Butylparaben 0.15 g Magnesium aluminum silicate 0.8 g Triethanolamine I g Cellulose gum 0.14 g Nylon- 12 8 g Cyclopentasiloxane 5 g Dimethicone 4 g Propylene glycol 3 g Glycerin 3 g Water 42.01 g Stearic acid 2 g Sodium lauroyl sarcosinate 3.5 g D-panthenol 0.5 g Example IV: Eyeshadow Talc 10 Moos 9.3 g Anti-keratin antibody coupled to mango violet 14 g Anti-keratin antibody coupled to brilliant ultramarine blue W 798 25 g Anti-keratin antibody coupled to carmine covalac W 3508 3 g Anti-k6ratine antibody coupled to black ariabel 2 g Mica concord 1000 5 g Pea rl glo UVR 20 g Zinc stearate 4 g Luxelen silk D 2 g Microspheres M 305 10 g Nipagine 0.2 g Binder (I) 5.5 g The binder (I) has the following composition: Cetyldimethicone 12.3% Dimethicone and trimethylsiloxysilicate 19.8% Dimethicone 62.9% 7 Triisoketyl citrate 5%

Claims (28)

  1. Antibody comprising a protein domain allowing the recognition of an antigen, characterized in that said antibody is coupled to a pigment and / or a dye and / or a cosmetic or dermatological active ingredient. 2. Antibody according to claim 1, characterized in that said antigen is an epitope of the skin and / or nails and / or   lips and / or eyelashes and / or hair.   3. Antibody according to claim 1 or 2, characterized in that   that said antigen is an epitope of keratin.   4. Antibody according to claim 1, characterized in that said antigen is an epitope of a second antibody comprising a protein domain allowing the recognition of an epitope of the   skin and / or nails and / or lips and / or eyelashes and / or hair.   5. Antibodies according to any one of claims 1 to 4,   characterized in that said antibody is coupled to a selected pigment   among mineral or organic pigments, white, colored or pearlescent.   6. Antibody according to any one of claims 1 to 4,   characterized in that said antibody is coupled to a dye chosen from paraphenylenediamines, para-aminophenols, orthophenylenediamines, heterocyclic bases and liposoluble dyes.   7. Antibody according to any one of claims I to 4,   characterized in that said antibody is coupled to a cosmetic or dermatological active agent chosen from agents modulating differentiation and / or proliferation and / or skin pigmentation, antibacterials, antiparasitics, antifungals, steroidal anti-inflammatory agents, non-steroidal anti-inflammatory agents, anesthetic agents, antipruritic agents, antiviral agents, keratolytic agents, anti-free radical agents, antiseborrheics, anti-dandruff, anti-acne, anti-metabolites, anti-hair loss agents , antiseptics, organic or mineral filters, layer restructuring agents   cornea, moisturizers and nourishing active ingredients.   8. Method for obtaining antibodies as defined in one   any one of claims 1 to 7, characterized in that it consists of   obtain antibodies comprising a protein domain allowing the recognition of an antigen, to purify said antibodies thus obtained, then to couple these antibodies with a pigment and / or a dye and / or a cosmetic or dermatological active agent and to isolate the antibodies thus coupled.   9. Method according to claim 8, characterized in that the antibodies comprising a protein domain allowing the recognition of an antigen are obtained by immunizing a mammal with said antigen then by taking the blood from said immune mammal.   10. The method of claim 8, characterized in that the antibodies comprising a protein domain allowing the recognition of an antigen are obtained by immunizing a mammal with said antigen, by removing the spleen cells from said mammal, by fusing the latter with immortalized cells and by collecting the antibodies synthesized by hybridomas thus obtained.   1 1. Method according to any one of claims 8 to 10,   characterized in that the antibodies are purified by precipitation with ammonium sulfate followed by exclusion chromatography, by affinity chromatography on protein A-sepharose, by affinity chromatography on protein G-sepharose by affinity chromatography on immunoglobulin light chain   (kappa chain) -sepharose or by ion exchange chromatography.   12. Method according to any one of claims 8 to 11,   characterized in that the antibodies are coupled with a pigment and / or a dye and / or a cosmetic or dermatological active ingredient using a fixing buffer allowing the fixing of said pigment and / or   dye and / or cosmetic or dermatological active on the antibody.   13. Method according to any one of claims 8 to 12,   characterized in that said antibodies coupled to a pigment and / or a dye and / or a cosmetic or dermatological active ingredient are isolated by dialysis. 14. Composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium, characterized in that it comprises, in addition, at least one   antibody as defined in any one of claims 1 to   7. 15. Composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium, characterized in that it comprises, in addition, at least one   antibody according to any one of claims 4 to 7, and at least   said second antibody comprising a protein domain allowing the recognition of an epitope of the skin and / or nails and / or   lips and / or eyelashes and / or hair.   16. Composition according to Claim 14, characterized in that the antibodies are present in proportions of between 0.001% to 80%, preferably from 0.01 to 60% v and more preferably from 1 to 50% by weight relative to the total weight of the composition. 17. Composition according to claim 15, characterized in that   that the antibodies according to any one of claims 4 to 7 are   present in proportions of between 0.001 to 80 /%, preferably between 0.01 to 60% and more preferably between 1 to 50% by weight relative to the total weight of the composition and in that said second antibodies are present in proportions between 0.001 to 80%, preferably between 0.01 to 60% and more preferably between 1 to 50% o by weight relative to the weight total of the composition.   18. Composition according to any one of claims 14   to 17, characterized in that the cosmetic, dermatological,   hygienic or pharmaceutical comprises at least one fatty phase.   19. Composition according to Claim 18, characterized in that the fatty phase is chosen from waxes, oils, gums and / or pasty fatty substances, of vegetable, animal, mineral or   synthetic, or silicone, and mixtures thereof.   20. Composition according to claim 18 or 19, characterized   in that the fatty phase is liquid.   21. Composition according to claim 20, characterized in that the liquid fatty phase is chosen from paraffin or petrolatum oil, hydrogenated polyisobutylene, mink, turtle, soybean oil, perhydrosqualene, oil sweet almond, calophyllum, palm, grapeseed, sesame, corn, parlam, arara, rapeseed, sunflower, cotton, apricot, castor, avocado, jojoba, olive or germ of cereals; esters of lanolic acid, oleic acid, lauric acid, stearic acid; fatty esters, such as isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, diisopropyl adipate, isononyl isononate, 2-ethyl-hexyl palmitate , 2-hexyl-decyl laurate,   2-octyl-decyl palmitate, myristate or 2-octyl-lactate   dodecyl, 2-diethyl-hexyl succinate, diisostearyl malate, glycerin or diglycerin triisostearate; higher fatty acids such as myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid or isostearic acid; higher fatty alcohols such as cetanol, stearyl alcohol or oleic alcohol, linoleic or linolenic alcohol, isostearic alcohol or octyl dodecanol; silicone oils such as PDMS, optionally phenylated, such as phenyltrimethicones, phenyltrimethylsiloxydiphenylsiloxanes, diphenylmethyl-dimethyltrisiloxanes, diphenyldimethicones, phenyldimethicones, polymethylphenylsiloxanes, or optionally arubstituted or optionally arubstituted or optionally aromatic / or optionally substituted by fluoropolymers or optionally substituted by aryl / phenylsiloxanes) functional groups such as hydroxyl, thiol and / or amine groups; polysiloxanes modified with fatty acids, fatty alcohols or polyoxalkylenes, fluorinated silicones, perfluorinated oils; volatile oils such as octamethylcyclotetrasiloxane, decamethylcyclopentasiloxane, hexadecamethylcyclohexasilone, heptamethylhexyltrisiloxane, heptamethyloctyltrisiloxane or   C8-C16 isoparaffins, organic solvents.   22. Composition according to any one of claims 18   to 21, in which the fatty phase contains at least one volatile oil at room temperature.   23. Composition according to any one of claims 18   to 22, in which the fatty phase contains homopolymers and   liposoluble and / or dispersible in the fatty phase copolymers.   24. Composition according to any one of claims   14 to 23, characterized in that the cosmetic, dermatological, hygienic or pharmaceutical medium comprises one or more pearlescent pigments or not and / or one or more liposoluble dyes and / or fillers.   25. Composition according to any one of claims 14   to 24, characterized in that the cosmetic, dermatological, hygienic or pharmaceutical medium contains at least one cosmetic active or dermatological.   26. Composition according to any one of claims 14   to 25, in the form of a stick or stick; in the form of a flexible paste, of dynamic viscosity at 25 C of the order of 1 to 40 Pa.s; in the form of a dish; oily gel; oily liquid; of vesicular dispersion containing ionic lipids and / or not   ionic, water-in-oil or oil-in-water emulsion.   27. Composition according to any one of claims 14   to 26, in the form of a care and / or makeup product   skin and / or lips and / or hair.   28. Use in or for the manufacture of a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium of an antibody such as   defined in any one of claims 1 to 7 to decrease,   see delete, transfer of said composition to application topical.   29. Use in or for the manufacture of a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium of an antibody such as   defined in any one of claims 1 to 4 coupled to an active   cosmetic or dermatological as defined in claim 7,   to enhance the action of said cosmetic or dermatological active ingredient.   30. Use in or for the manufacture of a composition for topical application, comprising a cosmetic, dermatological, hygienic or pharmaceutical medium of an antibody as defined in one   any of claims 1 to 4 coupled with a pigment and / or a   dye and / or cosmetic or dermatological active ingredient as defined   respectively in claims 5 to 7, to avoid penetration   said pigment and / or dye and / or cosmetic or dermatological active ingredient in the dermis.   31. Method for cosmetic care or for making up the lips, the skin or the hair, consisting in applying to the lips, the skin or the hair respectively, a cosmetic composition such as   defined in any one of claims 14 to 27.   32. A method of cosmetic care or of making up the lips, skin or hair, comprising applying to, respectively, the lips, the skin or the hair, a composition for topical application comprising a cosmetic, dermatological, hygienic or pharmaceutical medium and , in addition, at least one antibody comprising a protein domain allowing the recognition of an epitope, respectively, of the lips, the skin or the hair, then in applying a second composition for topical application, comprising a cosmetic, dermatological, hygienic medium or pharmaceutical and, in addition,   at least one antibody according to any one of claims 4 to 7,   recognizing the first antibody applied.