FR2750705B1 - Systeme de detection des interactions proteine-proteine - Google Patents

Systeme de detection des interactions proteine-proteine

Info

Publication number
FR2750705B1
FR2750705B1 FR9608337A FR9608337A FR2750705B1 FR 2750705 B1 FR2750705 B1 FR 2750705B1 FR 9608337 A FR9608337 A FR 9608337A FR 9608337 A FR9608337 A FR 9608337A FR 2750705 B1 FR2750705 B1 FR 2750705B1
Authority
FR
France
Prior art keywords
protein
detection system
interaction detection
protein interaction
interaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9608337A
Other languages
English (en)
Other versions
FR2750705A1 (fr
Inventor
Michele Granger
Manfred Schnarr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR9608337A priority Critical patent/FR2750705B1/fr
Priority to PCT/FR1997/001133 priority patent/WO1998001579A1/fr
Priority to EP97930586A priority patent/EP0909331A1/fr
Publication of FR2750705A1 publication Critical patent/FR2750705A1/fr
Application granted granted Critical
Publication of FR2750705B1 publication Critical patent/FR2750705B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FR9608337A 1996-07-04 1996-07-04 Systeme de detection des interactions proteine-proteine Expired - Fee Related FR2750705B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR9608337A FR2750705B1 (fr) 1996-07-04 1996-07-04 Systeme de detection des interactions proteine-proteine
PCT/FR1997/001133 WO1998001579A1 (fr) 1996-07-04 1997-06-25 Systeme de detection des interactions proteine-proteine
EP97930586A EP0909331A1 (fr) 1996-07-04 1997-06-25 Systeme de detection des interactions proteine-proteine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9608337A FR2750705B1 (fr) 1996-07-04 1996-07-04 Systeme de detection des interactions proteine-proteine

Publications (2)

Publication Number Publication Date
FR2750705A1 FR2750705A1 (fr) 1998-01-09
FR2750705B1 true FR2750705B1 (fr) 1998-10-30

Family

ID=9493710

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9608337A Expired - Fee Related FR2750705B1 (fr) 1996-07-04 1996-07-04 Systeme de detection des interactions proteine-proteine

Country Status (3)

Country Link
EP (1) EP0909331A1 (fr)
FR (1) FR2750705B1 (fr)
WO (1) WO1998001579A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2861742B1 (fr) * 2003-10-31 2006-02-24 Centre Nat Rech Scient LIGANDS DU REPRESSEUR MYCOBATERIEN EthR, PROCEDES DE SELECTION ET APPLICATIONS
FR2861745A1 (fr) * 2003-10-31 2005-05-06 Centre Nat Rech Scient Ligands du repressseur mycobacterien ethr, procedes de selection et applications
US8383589B2 (en) 2007-07-09 2013-02-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Aegyptin and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
NZ257181A (en) * 1992-10-29 1997-07-27 Medical Res Council Transcription factor dp-1, dna, vectors and host cells therefor

Also Published As

Publication number Publication date
FR2750705A1 (fr) 1998-01-09
EP0909331A1 (fr) 1999-04-21
WO1998001579A1 (fr) 1998-01-15

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Legal Events

Date Code Title Description
CL Concession to grant licences
ST Notification of lapse