FR2743891B1 - METHOD FOR THE IDENTIFICATION OR IMMUNOASSAY OF POLYPEPTIDE ANTIGENS - Google Patents

METHOD FOR THE IDENTIFICATION OR IMMUNOASSAY OF POLYPEPTIDE ANTIGENS

Info

Publication number
FR2743891B1
FR2743891B1 FR9600894A FR9600894A FR2743891B1 FR 2743891 B1 FR2743891 B1 FR 2743891B1 FR 9600894 A FR9600894 A FR 9600894A FR 9600894 A FR9600894 A FR 9600894A FR 2743891 B1 FR2743891 B1 FR 2743891B1
Authority
FR
France
Prior art keywords
immunoassay
specific
polypeptide antigen
sample
identification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9600894A
Other languages
French (fr)
Other versions
FR2743891A1 (en
Inventor
Frederic Jean
Gilles Kertesz
Beatrice Bourcier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immunotech SAS
Original Assignee
Immunotech SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunotech SAS filed Critical Immunotech SAS
Priority to FR9600894A priority Critical patent/FR2743891B1/en
Priority to AT96430018T priority patent/ATE196362T1/en
Priority to ES96430018T priority patent/ES2151646T3/en
Priority to EP96430018A priority patent/EP0785432B1/en
Priority to DK96430018T priority patent/DK0785432T3/en
Priority to DE69610283T priority patent/DE69610283T2/en
Priority to CA002193717A priority patent/CA2193717A1/en
Priority to US08/773,498 priority patent/US5817468A/en
Priority to AU12214/97A priority patent/AU712896B2/en
Publication of FR2743891A1 publication Critical patent/FR2743891A1/en
Application granted granted Critical
Publication of FR2743891B1 publication Critical patent/FR2743891B1/en
Priority to GR20000402665T priority patent/GR3034962T3/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Immunoassay for identification or determination of a specific polypeptide antigen X (I) in a sample comprises treating the sample in vitro with ≥ 1 agent (II) which reacts with the epsilon -amino function of a lysine residue or with the alpha -amino residue of a peptide to couple a residue of (II) with (I) and form modified (I), i.e. polypeptide antigen Xm (I'). The immunoassay for the presence of (I) in the sample is then carried out using ≥ 1 antibody (III) specific for (I') but not specific for the modification itself. (III) is produced by immunisation against a polypeptide antigen Y (IV) identical with (I') or its antigenic fragment. Also claimed are:(a) hybridomas producing anti-ACTH monoclonal antibodies acylated by succinic anhydride, deposited at the CNCM on 12.12.95 under the numbers 1-1648, 1-1649 and 1-1650, and(b) the acylated anti-ACTH monoclonal antibodies produced by the hybridomas as in (a).
FR9600894A 1996-01-18 1996-01-18 METHOD FOR THE IDENTIFICATION OR IMMUNOASSAY OF POLYPEPTIDE ANTIGENS Expired - Fee Related FR2743891B1 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FR9600894A FR2743891B1 (en) 1996-01-18 1996-01-18 METHOD FOR THE IDENTIFICATION OR IMMUNOASSAY OF POLYPEPTIDE ANTIGENS
ES96430018T ES2151646T3 (en) 1996-01-18 1996-12-16 PROCEDURE FOR THE IMMUNOLOGICAL IDENTIFICATION OR DOSAGE OF POLYPEPTIDIC ANTIGENS.
EP96430018A EP0785432B1 (en) 1996-01-18 1996-12-16 Method for the immunological identification or determination of polypeptide antigens
DK96430018T DK0785432T3 (en) 1996-01-18 1996-12-16 Method for immunological identification or concentration determination of polypeptide antigens
DE69610283T DE69610283T2 (en) 1996-01-18 1996-12-16 Method for the immunological identification or determination of polypeptide antigens
AT96430018T ATE196362T1 (en) 1996-01-18 1996-12-16 METHOD FOR THE IMMUNOLOGICAL IDENTIFICATION OR DETERMINATION OF POLYPEPTIDE ANTIGENS
CA002193717A CA2193717A1 (en) 1996-01-18 1996-12-20 Process for identification or immunologic determination of polypeptidic antigens
US08/773,498 US5817468A (en) 1996-01-18 1996-12-23 Process for the identification or immunoassay of polypeptide antigens
AU12214/97A AU712896B2 (en) 1996-01-18 1997-01-17 Process for the identification or immunoassay of polypeptide antigens
GR20000402665T GR3034962T3 (en) 1996-01-18 2000-11-30 Method for the immunological identification or determination of polypeptide antigens

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9600894A FR2743891B1 (en) 1996-01-18 1996-01-18 METHOD FOR THE IDENTIFICATION OR IMMUNOASSAY OF POLYPEPTIDE ANTIGENS

Publications (2)

Publication Number Publication Date
FR2743891A1 FR2743891A1 (en) 1997-07-25
FR2743891B1 true FR2743891B1 (en) 1998-04-10

Family

ID=9488489

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9600894A Expired - Fee Related FR2743891B1 (en) 1996-01-18 1996-01-18 METHOD FOR THE IDENTIFICATION OR IMMUNOASSAY OF POLYPEPTIDE ANTIGENS

Country Status (10)

Country Link
US (1) US5817468A (en)
EP (1) EP0785432B1 (en)
AT (1) ATE196362T1 (en)
AU (1) AU712896B2 (en)
CA (1) CA2193717A1 (en)
DE (1) DE69610283T2 (en)
DK (1) DK0785432T3 (en)
ES (1) ES2151646T3 (en)
FR (1) FR2743891B1 (en)
GR (1) GR3034962T3 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2931481B1 (en) * 2008-05-23 2013-05-10 Covalab PROPIONYLE / BUTYRYLEE LYSINE-SPECIFIC ANTIBODY, METHOD FOR PRODUCING THE SAME, AND APPLICATIONS THEREOF
CA2940242A1 (en) 2014-02-20 2015-08-27 Alder Biopharmaceuticals, Inc. Anti-acth antibodies and use thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE821053A (en) * 1974-08-01 1975-04-14 PRODUCT FOR DIAGNOSIS BY IMMUNOCHEMICAL DETERMINATION
GB2090598B (en) * 1980-12-11 1984-03-21 Mini Agriculture & Fisheries Enzyme-immunoassay method
US4415546A (en) * 1981-05-12 1983-11-15 Janakiraman Ramachandran Biologically active analogs of ACTH and radioimmuno assay therefor
FR2605109B1 (en) * 1986-10-13 1989-02-03 Ire Medgenix Sa PARATHORMONE ASSAY PROCESS
JPH0614047B2 (en) * 1987-02-27 1994-02-23 イーストマン コダック カンパニー Agglutination reagent and method for producing the same
FR2658301A1 (en) * 1990-02-14 1991-08-16 Caen Labo Biochimie Inst Rech Method for detection and analysis of cell degeneration

Also Published As

Publication number Publication date
DE69610283D1 (en) 2000-10-19
US5817468A (en) 1998-10-06
AU712896B2 (en) 1999-11-18
EP0785432A1 (en) 1997-07-23
CA2193717A1 (en) 1997-07-19
ATE196362T1 (en) 2000-09-15
DE69610283T2 (en) 2001-03-29
FR2743891A1 (en) 1997-07-25
GR3034962T3 (en) 2001-02-28
DK0785432T3 (en) 2001-01-22
EP0785432B1 (en) 2000-09-13
ES2151646T3 (en) 2001-01-01
AU1221497A (en) 1997-07-31

Similar Documents

Publication Publication Date Title
KR890701632A (en) Monoclonal Antibodies to Human Interleukin-4 and Hybridomas Producing the Same
IL116617A0 (en) Monoclonal antibodies specific for advanced glycosylation endproducts in biological samples
GB2246780B (en) Immunodiagnostic assay for rheumatoid arthritis
KR900015756A (en) Monoclonal antibodies
JPS6428560A (en) Immunometric measurement
AU635243B2 (en) Immunoassay of human osteocalcin, reagent and kit therefor, antihuman osteocalcin antibody, hybridoma producing said antibody, and method of producing said antibody
FR2743891B1 (en) METHOD FOR THE IDENTIFICATION OR IMMUNOASSAY OF POLYPEPTIDE ANTIGENS
DE69633976D1 (en) MONOCLONAL ANTIBODIES AND ANTIGENS RELATING TO HUMAN LUNG ADENOCARCINOMA AND IMMUNOASSAY METHOD USING THEREOF
ES2171639T3 (en) IMMUNOLOGICAL VALUATION PROCEDURE OF CORTISOL, MAINLY THE CONTENT IN THE URINE, AND REAGENTS USED.
ZA93123B (en) Peptides representing fragments of CMP, antibodies directed against the said peptides, and their uses
CA2054689A1 (en) An immunoassay method for determining the specificity of binding of a monoclonal antibody to an antigen
NO174005C (en) Monoclonal antibody, hybridoma, and reagent for immunological analysis of x-hANP
Würzner et al. Determination of the epitope specificities of monoclonal antibodies using unprocessed supernatants of hybridoma cultures
DE69012572D1 (en) MONOCLONAL ANTIBODY AGAINST ACETYLATED LYSINE WASTE IN RECOMBINANT RIND SOMATOTROPINE.
Kyurkchiev et al. Application of an immunological method to detect trace amounts of dried human semen
ZA861550B (en) Process and reagent for the determination of the luteinising hormone and monoclonal antibodies suitable therefor
Poltoranina et al. Mixed precipitation in gel: A new method of identification and characterization of monoclonal antibodies
EP0186371A3 (en) Monoclonal antibodies specific to antigens of hepatitis b virus
BG51530A1 (en) Hybridome cell line prodicing conoclonal antibody against h6-antigenous determinant of h1, 6-antigenous complex
IT1268561B1 (en) Method for the identification of grape virus GVA, monoclonal antibodies (MAb) against GVA and a hybridoma cell line capable of producing the said monoclonal antibodies
BG51638A1 (en) Hybridome cellular line mus musculus balb/c, secreting monoclonal antibody towards antigenous determinant, common for the strains of the virus of haemorrhagic fever with nephritic syndrome " hantaan " , " bulgarian " and " finnish ".

Legal Events

Date Code Title Description
ST Notification of lapse