FR2636643B1 - PEPTIDE-SIGNAL, DNA SEQUENCES ENCODING SAME, EXPRESSION VECTORS CARRYING ONE OF SUCH SEQUENCES, AND METHOD FOR PERIPLASMIC PRODUCTION OF A POLYPEPTIDE - Google Patents

PEPTIDE-SIGNAL, DNA SEQUENCES ENCODING SAME, EXPRESSION VECTORS CARRYING ONE OF SUCH SEQUENCES, AND METHOD FOR PERIPLASMIC PRODUCTION OF A POLYPEPTIDE

Info

Publication number
FR2636643B1
FR2636643B1 FR8811188A FR8811188A FR2636643B1 FR 2636643 B1 FR2636643 B1 FR 2636643B1 FR 8811188 A FR8811188 A FR 8811188A FR 8811188 A FR8811188 A FR 8811188A FR 2636643 B1 FR2636643 B1 FR 2636643B1
Authority
FR
France
Prior art keywords
polypeptide
peptide
expression vectors
vectors carrying
signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR8811188A
Other languages
French (fr)
Other versions
FR2636643A1 (en
Inventor
Richard Legoux
Pascal Leplatois
Evelyne Joseph-Liauzun
Gerard Loison
Willem Roskam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi SA
Original Assignee
Sanofi SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi SA filed Critical Sanofi SA
Priority to FR8811188A priority Critical patent/FR2636643B1/en
Priority to PT91511A priority patent/PT91511B/en
Priority to ES89402328T priority patent/ES2074087T3/en
Priority to EP89402328A priority patent/EP0356335B1/en
Priority to AT89402328T priority patent/ATE122398T1/en
Priority to DE68922549T priority patent/DE68922549T2/en
Priority to CA000609139A priority patent/CA1334944C/en
Priority to JP1220106A priority patent/JP2535076B2/en
Publication of FR2636643A1 publication Critical patent/FR2636643A1/en
Application granted granted Critical
Publication of FR2636643B1 publication Critical patent/FR2636643B1/en
Priority to US07/825,080 priority patent/US5284768A/en
Priority to GR950402097T priority patent/GR3016981T3/en
Priority to LV960298A priority patent/LV5810B4/en
Priority to BR1100350-2A priority patent/BR1100350A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a signal peptide of formula: MXKSTLLLLFLLLCLPSWNAGA where A = alanine C = cysteine F = phenylalanine G = glycine K = lysine L = leucine M = methionine N = asparagine P = proline S = serine T = threonine W = triptophane and X denotes a direct bond between M and K, an amino acid chosen from the group of the 20 amino acids of the genetic code or a peptide containing 2, 3 or 4 amino acids, each chosen independently of the others from the group of the 20 amino acids of the genetic code. It also relates to the DNA sequences coding for the latter, the expression vectors carrying one of these sequences, the Gram-negative bacteria transformed by these vectors and the process for periplasmic production of a polypeptide. Application: Manufacture of polypeptides such as hGH or hirudine.
FR8811188A 1988-08-24 1988-08-24 PEPTIDE-SIGNAL, DNA SEQUENCES ENCODING SAME, EXPRESSION VECTORS CARRYING ONE OF SUCH SEQUENCES, AND METHOD FOR PERIPLASMIC PRODUCTION OF A POLYPEPTIDE Expired - Lifetime FR2636643B1 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
FR8811188A FR2636643B1 (en) 1988-08-24 1988-08-24 PEPTIDE-SIGNAL, DNA SEQUENCES ENCODING SAME, EXPRESSION VECTORS CARRYING ONE OF SUCH SEQUENCES, AND METHOD FOR PERIPLASMIC PRODUCTION OF A POLYPEPTIDE
PT91511A PT91511B (en) 1988-08-24 1989-08-22 PROCESS FOR PRODUCING DNA SEQUENCES ENCODING OF A PEPTIDE-SIGNAL, EXPRESSION VECTORS CARRYING OUT THOSE SEQUENCES, GRAM-NEGATIVE BACTERIA TRANSFORMED BY THESE VECTORS, AND PROCESS FOR THE PERIPLASMIC PRODUCTION OF A POLYPETHYDE
CA000609139A CA1334944C (en) 1988-08-24 1989-08-23 Signal peptide, dna sequences coding for the latter, expression vectors carrying one of these sequences, gram-negative bacteria transformed by these vectors, and process for the periplasmic production of a polypeptide
EP89402328A EP0356335B1 (en) 1988-08-24 1989-08-23 Signal peptide and DNA sequences coding thereof
AT89402328T ATE122398T1 (en) 1988-08-24 1989-08-23 SIGNAL PEPTIDE AND DNA CODING SEQUENCES THEREOF.
DE68922549T DE68922549T2 (en) 1988-08-24 1989-08-23 Signal peptide and DNA sequences coding for it.
ES89402328T ES2074087T3 (en) 1988-08-24 1989-08-23 PEPTIDE-SIGNAL AND DNA SEQUENCES CODING FOR THE SAME.
JP1220106A JP2535076B2 (en) 1988-08-24 1989-08-24 Signal peptide, DNA sequence, vector, bacterial, and polypeptide periplasmic production methods
US07/825,080 US5284768A (en) 1988-08-24 1992-01-24 Signal peptide, DNA sequences coding for the latter, expression vectors carrying one of these sequences, gram-negative bacteria transformed by these vectors, and process for the periplasmic production of a polypeptide
GR950402097T GR3016981T3 (en) 1988-08-24 1995-08-02 Signal peptide and DNA sequences coding thereof.
LV960298A LV5810B4 (en) 1988-08-24 1996-07-22 Signalpeptids and their coding DNA sequences
BR1100350-2A BR1100350A (en) 1988-08-24 1997-04-25 Signal peptide, DNA sequences encoding for it, expression vectors carrying one of these sequences and the process of periplasmic production of a polypeptide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8811188A FR2636643B1 (en) 1988-08-24 1988-08-24 PEPTIDE-SIGNAL, DNA SEQUENCES ENCODING SAME, EXPRESSION VECTORS CARRYING ONE OF SUCH SEQUENCES, AND METHOD FOR PERIPLASMIC PRODUCTION OF A POLYPEPTIDE

Publications (2)

Publication Number Publication Date
FR2636643A1 FR2636643A1 (en) 1990-03-23
FR2636643B1 true FR2636643B1 (en) 1990-12-28

Family

ID=9369498

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8811188A Expired - Lifetime FR2636643B1 (en) 1988-08-24 1988-08-24 PEPTIDE-SIGNAL, DNA SEQUENCES ENCODING SAME, EXPRESSION VECTORS CARRYING ONE OF SUCH SEQUENCES, AND METHOD FOR PERIPLASMIC PRODUCTION OF A POLYPEPTIDE

Country Status (9)

Country Link
EP (1) EP0356335B1 (en)
JP (1) JP2535076B2 (en)
AT (1) ATE122398T1 (en)
CA (1) CA1334944C (en)
DE (1) DE68922549T2 (en)
ES (1) ES2074087T3 (en)
FR (1) FR2636643B1 (en)
GR (1) GR3016981T3 (en)
PT (1) PT91511B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4009268A1 (en) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind SECRETION OF HIRUDIN DERIVATIVES
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
WO2001094418A2 (en) * 2000-06-05 2001-12-13 Corixa Corporation Leader peptides for enhancing secretion of recombinant protein from a host cell
FR2957598B1 (en) 2010-03-17 2015-12-04 Lfb Biotechnologies NOVEL SIGNAL PEPTIDE AND USE THEREOF FOR THE PRODUCTION OF RECOMBINANT PROTEINS

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0170266B1 (en) * 1984-07-30 1993-03-24 Wakunaga Seiyaku Kabushiki Kaisha Process for producing protein, and vector, recombinant dna and transformant used therefor
EP0177343B1 (en) * 1984-10-05 1992-07-22 Genentech, Inc. Dna, cell cultures and methods for the secretion of heterologous proteins and periplasmic protein recovery

Also Published As

Publication number Publication date
CA1334944C (en) 1995-03-28
PT91511B (en) 1995-07-18
DE68922549D1 (en) 1995-06-14
EP0356335A1 (en) 1990-02-28
DE68922549T2 (en) 1996-01-18
PT91511A (en) 1990-03-08
GR3016981T3 (en) 1995-11-30
ATE122398T1 (en) 1995-05-15
JPH02257883A (en) 1990-10-18
JP2535076B2 (en) 1996-09-18
FR2636643A1 (en) 1990-03-23
ES2074087T3 (en) 1995-09-01
EP0356335B1 (en) 1995-05-10

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