FR2452516A1 - NOVEL ESSENTIALLY PURE PLASMID AND ITS ISOLATION METHOD - Google Patents

NOVEL ESSENTIALLY PURE PLASMID AND ITS ISOLATION METHOD

Info

Publication number
FR2452516A1
FR2452516A1 FR8006945A FR8006945A FR2452516A1 FR 2452516 A1 FR2452516 A1 FR 2452516A1 FR 8006945 A FR8006945 A FR 8006945A FR 8006945 A FR8006945 A FR 8006945A FR 2452516 A1 FR2452516 A1 FR 2452516A1
Authority
FR
France
Prior art keywords
plasmid
puc1
essentially pure
isolation method
pure plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR8006945A
Other languages
French (fr)
Other versions
FR2452516B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia and Upjohn Co
Original Assignee
Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upjohn Co filed Critical Upjohn Co
Publication of FR2452516A1 publication Critical patent/FR2452516A1/en
Application granted granted Critical
Publication of FR2452516B1 publication Critical patent/FR2452516B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/54—Streptomyces fradiae

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'INVENTION CONCERNE A TITRE DE COMPOSE CHIMIQUE NOUVEAU LE PLASMIDE PUC1 ESSENTIELLEMENT PUR. LE PLASMIDE PUC1 EST OBTENU A PARTIR D'UNE CULTURE BIOLOGIQUEMENT PURE DU MICRO-ORGANISME STREPTOMYCES FRADIAE NRRL 11443. LE PLASMIDE PUC1 EST UTILE COMME VEHICULE DE CLONAGE DANS LES TRAVAUX DE RECOMBINAISON DE L'ADN. PAR EXEMPLE, EN UTILISANT LA METHODOLOGIE DE L'ADN, ONT PEUT INSERER UN GENE DESIRE, PAR EXEMPLE LE GENE DE L'INSULINE, DANS PUC1 ET ON PEUT ENSUITE TRANSFORMER LE PLASMIDE RESULTANT EN UN HOTE CONVENABLE QUI, PAR CULTURE, PRODUIT L'INSULINE DESIREE.THE INVENTION CONCERNS, AS A NEW CHEMICAL COMPOUND, ESSENTIALLY PURE PLASMID PUC1. PUC1 PLASMID IS OBTAINED FROM A BIOLOGICALLY PURE CULTURE OF THE MICRO-ORGANISM STREPTOMYCE FRADIAE NRRL 11443. PUC1 PLASMID IS USEFUL AS A CLONING VEHICLE IN DNA RECOMBINATION WORK. FOR EXAMPLE, USING THE DNA METHODOLOGY, ONE CAN INSERT A DESIRED GENE, FOR EXAMPLE THE GENE OF INSULIN, INTO PUC1 AND THE RESULTING PLASMID CAN THEN BE TRANSFORMED TO A SUITABLE HOST WHICH, BY CULTURE, PRODUCES THE DESIRED INSULIN.

FR8006945A 1979-03-29 1980-03-28 NOVEL ESSENTIALLY PURE PLASMID AND ITS ISOLATION METHOD Granted FR2452516A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2488779A 1979-03-29 1979-03-29

Publications (2)

Publication Number Publication Date
FR2452516A1 true FR2452516A1 (en) 1980-10-24
FR2452516B1 FR2452516B1 (en) 1984-03-16

Family

ID=21822884

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8006945A Granted FR2452516A1 (en) 1979-03-29 1980-03-28 NOVEL ESSENTIALLY PURE PLASMID AND ITS ISOLATION METHOD

Country Status (6)

Country Link
JP (1) JPS55133396A (en)
DE (1) DE3008647A1 (en)
FR (1) FR2452516A1 (en)
GB (1) GB2045253B (en)
IT (1) IT1129736B (en)
NL (1) NL8001240A (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57206699A (en) * 1981-06-10 1982-12-18 Takeda Chem Ind Ltd Plasmid patm 3 and its preparation
US4460691A (en) * 1981-06-29 1984-07-17 The Upjohn Company Streptomyces plasmid prophage pUC13
US4703009A (en) * 1983-03-08 1987-10-27 Merck & Co., Inc. RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes
JP2619769B2 (en) * 1992-07-15 1997-06-11 特種製紙株式会社 Transparent paper manufacturing method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CA1979 *
EXBK/78 *
EXBK/80 *

Also Published As

Publication number Publication date
JPS55133396A (en) 1980-10-17
DE3008647A1 (en) 1980-10-09
IT8020456A0 (en) 1980-03-07
IT1129736B (en) 1986-06-11
GB2045253A (en) 1980-10-29
GB2045253B (en) 1982-11-10
NL8001240A (en) 1980-10-01
FR2452516B1 (en) 1984-03-16

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Legal Events

Date Code Title Description
ST Notification of lapse