FR2436993A1 - METHOD AND COMPOSITION FOR THE COLORING AND ANALYSIS OF BACTERIA - Google Patents

METHOD AND COMPOSITION FOR THE COLORING AND ANALYSIS OF BACTERIA

Info

Publication number
FR2436993A1
FR2436993A1 FR7923676A FR7923676A FR2436993A1 FR 2436993 A1 FR2436993 A1 FR 2436993A1 FR 7923676 A FR7923676 A FR 7923676A FR 7923676 A FR7923676 A FR 7923676A FR 2436993 A1 FR2436993 A1 FR 2436993A1
Authority
FR
France
Prior art keywords
bacteria
gram
composition
staining
concentrated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7923676A
Other languages
French (fr)
Other versions
FR2436993B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baylor College of Medicine
Original Assignee
Baylor College of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/033,900 external-priority patent/US4225669A/en
Application filed by Baylor College of Medicine filed Critical Baylor College of Medicine
Publication of FR2436993A1 publication Critical patent/FR2436993A1/en
Application granted granted Critical
Publication of FR2436993B1 publication Critical patent/FR2436993B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'INVENTION CONCERNE UNE COMPOSITION DE COLORATION DES BACTERIES ET DES METHODES D'ANALYSE DES BACTERIES GRAM-NEGATIVES ET GRAM-POSITIVES. LA COMPOSITION CONTIENT UN AGENT CHELATANT ET UN COLORANT BASIQUE, TOUS LES DEUX ACTIFS A UN PH SUPERIEUR A 7,0. LA COLORATION DES BACTERIES PEUT SE FAIRE EN METTANT DES BACTERIES CONCENTREES OU EN SUSPENSION DANS UN FLUIDE, EN CONTACT AVEC LA COMPOSITION A UN PH NEUTRE OU BASIQUE. LES BACTERIES COLOREES AVEC LA COMPOSITION ET CONCENTREES PAR FILTRATION, CENTRIFUGATION OU UNE TECHNIQUE SIMILAIRE, SONT FACILEMENT VISIBLES ET LEUR PRESENCE DANS UN ECHANTILLON PEUT AINSI ETRE RAPIDEMENT DETECTEE. LES GRADATIONS DE COULEUR DES BACTERIES COLOREES, CONCENTREES, CORRESPONDENT AU NOMBRE DE BACTERIES ET UNE ANALYSE SEMI-QUANTITATIVE DES BACTERIES PEUT ETRE EFFECTUEE PAR COMPARAISON AVEC UN TEMOIN DE REFERENCE. LA DIFFERENCIATION DES BACTERIES GRAM-NEGATIVES ET GRAM-POSITIVES PEUT ETRE EFFECTUEE EN TRAITANT LES BACTERIES COLOREES PAR UNE SOLUTION D'UN ACIDE ORGANIQUE AYANT UN PH DE 2,5 A 2,6 ENVIRON. UN TEL LAVAGE DECOLORE COMPLETEMENT LES BACTERIES GRAM-POSITIVES. ENFIN, ON FOURNIT UN PROCEDE DE DETERMINATION DE LA SENSIBILITE DES BACTERIES VIS-A-VIS DES AGENTS ANTIMICROBIENS, QUI CONSISTE A INCUBER LES BACTERIES AVEC UN AGENT ANTIMICROBIEN, A COLORER LES BACTERIES, PUIS A COMPARER LA GRADATION DE COULEUR DES BACTERIES COLOREES, CONCENTREES, AVEC UN TEMOIN OU UN PRODUIT DE REFERENCE.THE INVENTION RELATES TO A STAINING COMPOSITION OF BACTERIA AND METHODS OF ANALYSIS OF GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA. THE COMPOSITION CONTAINS A CHELATING AGENT AND A BASIC COLORANT, BOTH ACTIVE AT A PH GREATER THAN 7.0. STAINING OF BACTERIA CAN BE ACHIEVED BY PLACING CONCENTRATED OR SUSPENDED BACTERIA IN A FLUID, IN CONTACT WITH THE COMPOSITION AT A NEUTRAL OR BASIC PH. BACTERIA COLORED WITH THE COMPOSITION AND CONCENTRATED BY FILTRATION, CENTRIFUGATION OR SIMILAR TECHNIQUE, ARE EASILY VISIBLE AND THEIR PRESENCE IN A SAMPLE CAN THUS BE QUICKLY DETECTED. THE COLOR GRADATIONS OF COLORED, CONCENTRATED BACTERIA CORRESPOND TO THE NUMBER OF BACTERIA AND A SEMI-QUANTITATIVE ANALYSIS OF THE BACTERIA CAN BE PERFORMED BY COMPARISON WITH A REFERENCE CONTROL. THE DIFFERENTIATION OF GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA CAN BE CARRIED OUT BY TREATING THE STAINED BACTERIA WITH AN ORGANIC ACID SOLUTION WITH A PH OF APPROXIMATELY 2.5 TO 2.6. SUCH A WASH COMPLETELY DISCOLORS GRAM-POSITIVE BACTERIA. FINALLY, A PROCESS FOR DETERMINING THE SENSITIVITY OF BACTERIA TO ANTIMICROBIAL AGENTS IS PROVIDED, WHICH CONSISTS OF INCUBATING THE BACTERIA WITH AN ANTIMICROBIAL AGENT, STAINING THE BACTERIA, THEN COMPARING THE COLORING OF BACTERIA, CONCENTRATE COLOR , WITH A WITNESS OR A REFERENCE PRODUCT.

FR7923676A 1978-09-25 1979-09-24 METHOD AND COMPOSITION FOR THE COLORING AND ANALYSIS OF BACTERIA Granted FR2436993A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94519778A 1978-09-25 1978-09-25
US06/033,900 US4225669A (en) 1979-04-27 1979-04-27 Staining and analysis of bacteria

Publications (2)

Publication Number Publication Date
FR2436993A1 true FR2436993A1 (en) 1980-04-18
FR2436993B1 FR2436993B1 (en) 1983-04-29

Family

ID=26710302

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7923676A Granted FR2436993A1 (en) 1978-09-25 1979-09-24 METHOD AND COMPOSITION FOR THE COLORING AND ANALYSIS OF BACTERIA

Country Status (7)

Country Link
BR (1) BR7906097A (en)
CA (1) CA1112987A (en)
DE (2) DE2938511C2 (en)
FR (1) FR2436993A1 (en)
GB (1) GB2031457B (en)
IT (1) IT1164706B (en)
SE (1) SE7907581L (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0107594A1 (en) * 1982-10-26 1984-05-02 EASTMAN KODAK COMPANY (a New Jersey corporation) Method of discrimination of microorganisms, composition and element for the implantation thereof
EP0261934A2 (en) * 1986-09-24 1988-03-30 EASTMAN KODAK COMPANY (a New Jersey corporation) Rapid differentiation of bacteria using cyclic polypeptide antibiotics
EP0261933A2 (en) * 1986-09-24 1988-03-30 EASTMAN KODAK COMPANY (a New Jersey corporation) Rapid differentiation of bacteria using polyether antibiotics
EP0294529A1 (en) * 1987-06-12 1988-12-14 HYMAN, Edward S. Determination of bacteriuria

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4617264A (en) * 1983-11-04 1986-10-14 Syntex (U.S.A.) Inc. Pretreatment method and composition
AU2002224757A1 (en) * 2001-01-17 2002-07-30 Danish Dairy Board Method for differential analysis of bacteria in a sample
CH703678B1 (en) * 2004-04-06 2012-03-15 Empa Testmaterialien Ag Method and apparatus for testing bactericidal effect of substances.
CN112574863A (en) * 2019-09-29 2021-03-30 广东体必康生物科技有限公司 Bacteria detection method based on double-layer membrane filtration

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3043751A (en) * 1960-03-14 1962-07-10 Harold C Herman Process and equipment for determining microbial sensitivity to anti-microbial agents
US3554871A (en) * 1969-05-16 1971-01-12 American Sterilizer Co Strip for staining bacteria
FR2234368A1 (en) * 1973-06-22 1975-01-17 Univ Strathclyde Microscope estimation of microorganisms - by chemical modification and reaction with dye
FR2355912A1 (en) * 1976-06-22 1978-01-20 Univ Strathclyde MICROORGANISMS STAINING PROCESS

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD101759A1 (en) * 1973-01-28 1973-11-12

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3043751A (en) * 1960-03-14 1962-07-10 Harold C Herman Process and equipment for determining microbial sensitivity to anti-microbial agents
US3554871A (en) * 1969-05-16 1971-01-12 American Sterilizer Co Strip for staining bacteria
FR2234368A1 (en) * 1973-06-22 1975-01-17 Univ Strathclyde Microscope estimation of microorganisms - by chemical modification and reaction with dye
FR2355912A1 (en) * 1976-06-22 1978-01-20 Univ Strathclyde MICROORGANISMS STAINING PROCESS

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0107594A1 (en) * 1982-10-26 1984-05-02 EASTMAN KODAK COMPANY (a New Jersey corporation) Method of discrimination of microorganisms, composition and element for the implantation thereof
EP0261934A2 (en) * 1986-09-24 1988-03-30 EASTMAN KODAK COMPANY (a New Jersey corporation) Rapid differentiation of bacteria using cyclic polypeptide antibiotics
EP0261933A2 (en) * 1986-09-24 1988-03-30 EASTMAN KODAK COMPANY (a New Jersey corporation) Rapid differentiation of bacteria using polyether antibiotics
EP0261933A3 (en) * 1986-09-24 1989-10-11 EASTMAN KODAK COMPANY (a New Jersey corporation) Rapid differentiation of bacteria using polyether antibiotics
EP0261934A3 (en) * 1986-09-24 1989-10-11 EASTMAN KODAK COMPANY (a New Jersey corporation) Rapid differentiation of bacteria using cyclic polypeptide antibiotics
EP0294529A1 (en) * 1987-06-12 1988-12-14 HYMAN, Edward S. Determination of bacteriuria

Also Published As

Publication number Publication date
IT1164706B (en) 1987-04-15
DE2953720C2 (en) 1983-10-20
DE2938511A1 (en) 1980-03-27
IT7950319A0 (en) 1979-09-21
FR2436993B1 (en) 1983-04-29
GB2031457A (en) 1980-04-23
BR7906097A (en) 1980-05-27
GB2031457B (en) 1982-10-27
SE7907581L (en) 1980-03-26
CA1112987A (en) 1981-11-24
DE2938511C2 (en) 1982-09-16

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