FR2422699A1 - Materials for affinity chromatography of biological macromolecules - comprise support coated with polysaccharide coupled to amine - Google Patents

Materials for affinity chromatography of biological macromolecules - comprise support coated with polysaccharide coupled to amine

Info

Publication number
FR2422699A1
FR2422699A1 FR7810738A FR7810738A FR2422699A1 FR 2422699 A1 FR2422699 A1 FR 2422699A1 FR 7810738 A FR7810738 A FR 7810738A FR 7810738 A FR7810738 A FR 7810738A FR 2422699 A1 FR2422699 A1 FR 2422699A1
Authority
FR
France
Prior art keywords
amine
polysaccharide
materials
support coated
affinity chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR7810738A
Other languages
French (fr)
Inventor
Jean-Louis Tayot
Michel Tardy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Merieux SA
Original Assignee
Institut Merieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Merieux SA filed Critical Institut Merieux SA
Priority to FR7810738A priority Critical patent/FR2422699A1/en
Publication of FR2422699A1 publication Critical patent/FR2422699A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3253—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3278—Polymers being grafted on the carrier
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/328—Polymers on the carrier being further modified
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C12N11/12—Cellulose or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548—Carbohydrates, e.g. dextran
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58—Use in a single column

Abstract

Chromatographic materials capable of reversibly fixing biologically active macromolecules comprise a porous mineral support coated with an opt. modified, opt. crosslinked polysaccharide to which a chromatographically reactive amine is coupled. Coupling is effected by oxidative cleavage of the polysaccharide followed by reaction with the amine followed by redn. of the amine linkage to an amine linkage of formula R3-CH2-NH-R' (where R'NH is the residue of an amine molecule or macromolecule of formula R'NH2, and R3CH2- is the residue of the polysaccharide after oxidn. and redn.) The materials can be used for isolating or purifying enzymes, hormones, proteins, viruses, bacteria, antigens (esp. cholera toxin) or antibodies.
FR7810738A 1978-04-12 1978-04-12 Materials for affinity chromatography of biological macromolecules - comprise support coated with polysaccharide coupled to amine Withdrawn FR2422699A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7810738A FR2422699A1 (en) 1978-04-12 1978-04-12 Materials for affinity chromatography of biological macromolecules - comprise support coated with polysaccharide coupled to amine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7810738A FR2422699A1 (en) 1978-04-12 1978-04-12 Materials for affinity chromatography of biological macromolecules - comprise support coated with polysaccharide coupled to amine

Publications (1)

Publication Number Publication Date
FR2422699A1 true FR2422699A1 (en) 1979-11-09

Family

ID=9207007

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7810738A Withdrawn FR2422699A1 (en) 1978-04-12 1978-04-12 Materials for affinity chromatography of biological macromolecules - comprise support coated with polysaccharide coupled to amine

Country Status (1)

Country Link
FR (1) FR2422699A1 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2478104A1 (en) * 1980-03-17 1981-09-18 Merieux Inst NEW GANGLIOSIDE DERIVATIVES, THEIR PREPARATION AND APPLICATION
EP0103184A2 (en) * 1982-08-12 1984-03-21 Kanegafuchi Chemical Industry Co., Ltd. Activation of biocompatible terpolymers with biologicals whose binding complements are pathological effectors
EP0147804A2 (en) * 1983-12-28 1985-07-10 Daicel Chemical Industries, Ltd. Method of separating optical isomers and geometrical isomers
EP0245088A2 (en) * 1986-05-07 1987-11-11 Uop Inc. Regenerable support matrix for immobilizing biologically active materials
EP0268296A2 (en) * 1986-11-19 1988-05-25 Genetic Systems Corporation Membrane affinity concentration immunoassay
EP0278556A2 (en) * 1987-02-13 1988-08-17 Metallgesellschaft Ag Spherical biocatalyst and process for its preparation
EP0303262A2 (en) * 1987-08-13 1989-02-15 Forschungszentrum Jülich Gmbh Inorganic carrier with an amine-containing surface layer for the immobilization of microorganisms or cells, process for its production and use thereof
EP0320184A1 (en) * 1987-12-04 1989-06-14 Kuraray Co., Ltd. Agents for removing human immunodeficiency virus and/or its related compounds

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1271945A (en) * 1959-10-26 1961-09-15 Glaxo Lab Ltd New cellulose derivatives
FR2319399A1 (en) * 1975-07-29 1977-02-25 Merieux Inst NEW MATERIAL WITH CATIONIC CHARACTER, CAPABLE OF REVERSIBLE FIXING, BIOLOGICAL MACROMOLECULES, PREPARATION METHODS AND APPLICATIONS

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1271945A (en) * 1959-10-26 1961-09-15 Glaxo Lab Ltd New cellulose derivatives
FR2319399A1 (en) * 1975-07-29 1977-02-25 Merieux Inst NEW MATERIAL WITH CATIONIC CHARACTER, CAPABLE OF REVERSIBLE FIXING, BIOLOGICAL MACROMOLECULES, PREPARATION METHODS AND APPLICATIONS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CA1978 *

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2478104A1 (en) * 1980-03-17 1981-09-18 Merieux Inst NEW GANGLIOSIDE DERIVATIVES, THEIR PREPARATION AND APPLICATION
EP0036372A1 (en) * 1980-03-17 1981-09-23 Institut Merieux Ganglioside derivatives, their preparation and their application
EP0103184A2 (en) * 1982-08-12 1984-03-21 Kanegafuchi Chemical Industry Co., Ltd. Activation of biocompatible terpolymers with biologicals whose binding complements are pathological effectors
EP0103184A3 (en) * 1982-08-12 1985-06-19 Diamond Shamrock Chemicals Company Activation of biocompatible polymers with biologicals whose binding complements are pathological effectors
EP0147804A2 (en) * 1983-12-28 1985-07-10 Daicel Chemical Industries, Ltd. Method of separating optical isomers and geometrical isomers
EP0147804A3 (en) * 1983-12-28 1986-05-28 Daicel Chemical Industries, Ltd. Separating agent
EP0245088A2 (en) * 1986-05-07 1987-11-11 Uop Inc. Regenerable support matrix for immobilizing biologically active materials
EP0245088A3 (en) * 1986-05-07 1989-08-09 Uop Inc. Regenerable support matrix for immobilizing biologically active materials
EP0268296A2 (en) * 1986-11-19 1988-05-25 Genetic Systems Corporation Membrane affinity concentration immunoassay
EP0268296A3 (en) * 1986-11-19 1988-09-28 Genetic Systems Corporation Membrane affinity concentration immunoassay
EP0278556A2 (en) * 1987-02-13 1988-08-17 Metallgesellschaft Ag Spherical biocatalyst and process for its preparation
EP0278556A3 (en) * 1987-02-13 1989-08-09 Metallgesellschaft Ag Spherical biocatalyst and process for its preparation
EP0303262A2 (en) * 1987-08-13 1989-02-15 Forschungszentrum Jülich Gmbh Inorganic carrier with an amine-containing surface layer for the immobilization of microorganisms or cells, process for its production and use thereof
EP0303262A3 (en) * 1987-08-13 1990-01-31 Forschungszentrum Jülich Gmbh Inorganic carrier with an amine-containing surface layer for the immobilization of microorganisms or cells, process for its production and use thereof
EP0320184A1 (en) * 1987-12-04 1989-06-14 Kuraray Co., Ltd. Agents for removing human immunodeficiency virus and/or its related compounds

Similar Documents

Publication Publication Date Title
SE8200715L (en) PYROGEN CONTENT REDUCTION
Prisyazhnoy et al. Synthesis of high-capacity immunoaffinity sorbents with oriented immobilized immunoglobulins or their Fab′ fragments for isolation of proteins
Lotan et al. Purification of wheat germ agglutinin by affinity chromatography on a Sepharose-bound N-acetylglucosamine derivative
Ngo Facile activation of Sepharose hydroxyl groups by 2-fluoro-1 methylpyridinium toluene-4-sulfonate: preparation of affinity and covalent chromatographic matrices
ATE69310T1 (en) PROCESS FOR PREPARING A MATERIAL FOR AFFINITY CHROMATOGRAPHY.
GR3007381T3 (en)
NZ215557A (en) Purification of immunoglobulin g using liquid chromatography or solid phase extraction
FR2422699A1 (en) Materials for affinity chromatography of biological macromolecules - comprise support coated with polysaccharide coupled to amine
EP0194578A3 (en) Proteins immobilised on polyamides or cellulose hydrate and the use thereof for the preparation of biocatalysts, test strips or chromatography materials
DE69323713D1 (en) NEW CONNECTIONS AND CONJUGATES
KRUG et al. Glucagon affinity absorbents: selective binding of receptors of liver cell membranes
US4886755A (en) Preparation of polymeric thiol gels for covalent bonding of biologically active ligands
DE69032883D1 (en) ENZYMATIC BINDING OF BIOACTIVE MATERIALS TO PROTEINS
GB8927230D0 (en) Reagents
EP0057600A2 (en) Affinity resins and their use in isolating bacterial luciferase
ES2133743T3 (en) CHROMATOGRAPHIC MATERIAL.
US4360592A (en) Process for the detection of antibodies
EP0184361B1 (en) Method of activating polymeric carrier
US4822863A (en) Sulphuryl chloride/polyamide derivative
WO1999006446A3 (en) Method for transglutaminase-catalyzed coupling of protein or peptide to a support
PT81050A (en) Anti-urokinase monoclonal antibody and process for preparing biochemical test kits which use it
DE3779125D1 (en) CLEANING METHOD FOR ANTIBODIES.
DK544085D0 (en) PROCEDURE FOR THE MANUFACTURING OF A CARRIER IMMOBILIZED, HIGHLY EFFECTIVE, BIOLOGICAL EFFECT
SU979354A1 (en) Process for producing sorbent
SU883052A1 (en) Immunosorbent

Legal Events

Date Code Title Description
ST Notification of lapse