FR2420572A1 - Reagent for diagnosis of staphylococcus aureus - prepd. by sensitisation of red blood cells with protein a reactive immunoglobulin(s), then treatment with glutaraldehyde and lyophilisation - Google Patents

Reagent for diagnosis of staphylococcus aureus - prepd. by sensitisation of red blood cells with protein a reactive immunoglobulin(s), then treatment with glutaraldehyde and lyophilisation

Info

Publication number
FR2420572A1
FR2420572A1 FR7808748A FR7808748A FR2420572A1 FR 2420572 A1 FR2420572 A1 FR 2420572A1 FR 7808748 A FR7808748 A FR 7808748A FR 7808748 A FR7808748 A FR 7808748A FR 2420572 A1 FR2420572 A1 FR 2420572A1
Authority
FR
France
Prior art keywords
glutaraldehyde
red blood
sensitisation
diagnosis
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR7808748A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Recherche Agronomique INRA
Original Assignee
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Recherche Agronomique INRA filed Critical Institut National de la Recherche Agronomique INRA
Priority to FR7808748A priority Critical patent/FR2420572A1/en
Publication of FR2420572A1 publication Critical patent/FR2420572A1/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
    • G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Prepn. of a reagent for the diagnosis of Staph. aureus comprises (a) sensitising the red blood corpuscles of an animal with a serum contg. reactive immunoglobulins with respect to protein A and having an anti-red corpuscle activity for the said animal; (b) addn. of glutaraldehyde to the sensitised preparation and (c) lyophilisation of the preparation. Stable reagent having high sensitivity for the determination of Staph. aureus by haemagglutination. The diagnosis is very rapid about 30 seconds per test. Known reagents are unstable, diffult to prepare to give slow response times. The sensitisation in stage (a) is generally effected at 37-40 degrees C for 1-2 hrs. and stage (b) is effected at 37-40 degrees C for 10-15 hrs. after glutaraldehyde addition. Pref. the temp. is 40 degrees C, 0.0025-0.01 vol/vol.% of glutaraldehyde is used, the red blood corpuscles are sheep corpuscles and the serum used for the sensitisation is rabbit antisheep red blood corpuscle serum.
FR7808748A 1978-03-24 1978-03-24 Reagent for diagnosis of staphylococcus aureus - prepd. by sensitisation of red blood cells with protein a reactive immunoglobulin(s), then treatment with glutaraldehyde and lyophilisation Withdrawn FR2420572A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7808748A FR2420572A1 (en) 1978-03-24 1978-03-24 Reagent for diagnosis of staphylococcus aureus - prepd. by sensitisation of red blood cells with protein a reactive immunoglobulin(s), then treatment with glutaraldehyde and lyophilisation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7808748A FR2420572A1 (en) 1978-03-24 1978-03-24 Reagent for diagnosis of staphylococcus aureus - prepd. by sensitisation of red blood cells with protein a reactive immunoglobulin(s), then treatment with glutaraldehyde and lyophilisation

Publications (1)

Publication Number Publication Date
FR2420572A1 true FR2420572A1 (en) 1979-10-19

Family

ID=9206294

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7808748A Withdrawn FR2420572A1 (en) 1978-03-24 1978-03-24 Reagent for diagnosis of staphylococcus aureus - prepd. by sensitisation of red blood cells with protein a reactive immunoglobulin(s), then treatment with glutaraldehyde and lyophilisation

Country Status (1)

Country Link
FR (1) FR2420572A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989005980A1 (en) * 1987-12-24 1989-06-29 Institut Pasteur Reagent for the detection of staphylococcus aureus by agglutination

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2144229A5 (en) * 1971-05-06 1973-02-09 Leo Ab
FR2206859A5 (en) * 1972-11-06 1974-06-07 Pharmacia Ab

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2144229A5 (en) * 1971-05-06 1973-02-09 Leo Ab
FR2206859A5 (en) * 1972-11-06 1974-06-07 Pharmacia Ab

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989005980A1 (en) * 1987-12-24 1989-06-29 Institut Pasteur Reagent for the detection of staphylococcus aureus by agglutination
FR2625321A1 (en) * 1987-12-24 1989-06-30 Pasteur Institut REAGENT FOR THE DIAGNOSIS OF STAPHYLOCOCCUS AUREUS BY AGGLUTINATION
EP0323331A1 (en) * 1987-12-24 1989-07-05 Institut Pasteur Reagent for the detection of Staphylococcus aureus by agglutination
US6482602B1 (en) 1987-12-24 2002-11-19 Institut Pasteur Reagent for the detection of Staphylococcus aureus by agglutination
US6686169B2 (en) 1987-12-24 2004-02-03 Institut Pasteur Reagent for the detection of Staphylococcus aureus by agglutination

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Legal Events

Date Code Title Description
ST Notification of lapse