FR2385099A1 - Methode de determination immuno-enzymologique - Google Patents

Methode de determination immuno-enzymologique

Info

Publication number
FR2385099A1
FR2385099A1 FR7708389A FR7708389A FR2385099A1 FR 2385099 A1 FR2385099 A1 FR 2385099A1 FR 7708389 A FR7708389 A FR 7708389A FR 7708389 A FR7708389 A FR 7708389A FR 2385099 A1 FR2385099 A1 FR 2385099A1
Authority
FR
France
Prior art keywords
enzyme
support
marking
rapid
determn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7708389A
Other languages
English (en)
Other versions
FR2385099B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Diagnostica Stago SAS
Original Assignee
Diagnostica Stago SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diagnostica Stago SAS filed Critical Diagnostica Stago SAS
Priority to FR7708389A priority Critical patent/FR2385099A1/fr
Publication of FR2385099A1 publication Critical patent/FR2385099A1/fr
Application granted granted Critical
Publication of FR2385099B1 publication Critical patent/FR2385099B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G01N33/541—Double or second antibody, i.e. precipitating antibody

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La présente invention concerne une méthode de détermination immunoenzymologique pour le dosage d'une substance choisie parmi l'ensemble constitué par les substances antigéniques et les anticorps, dans laquelle on marque la substance à doser ou son opposé spécifique, au moyen d'un enzyme, notamment la peroxydase on forme un complexe antigène-anticorps comprenant la totalité ou une fraction de l'enzyme de marquage, on sépare ledit complexe antigène-anticorps notamment par fixation sur un support immuno-adsorbant, et on assure la réaction de mise en évidence de l'enzyme fixée sur la substance à doser, puis on procède à une élution du produit de réaction formé fixé sur le support immunoadsorbant, ladite méthode étant caractérisée en ce que l'élution est effectuée en milieu acide.
FR7708389A 1977-03-21 1977-03-21 Methode de determination immuno-enzymologique Granted FR2385099A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7708389A FR2385099A1 (fr) 1977-03-21 1977-03-21 Methode de determination immuno-enzymologique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7708389A FR2385099A1 (fr) 1977-03-21 1977-03-21 Methode de determination immuno-enzymologique

Publications (2)

Publication Number Publication Date
FR2385099A1 true FR2385099A1 (fr) 1978-10-20
FR2385099B1 FR2385099B1 (fr) 1982-02-26

Family

ID=9188353

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7708389A Granted FR2385099A1 (fr) 1977-03-21 1977-03-21 Methode de determination immuno-enzymologique

Country Status (1)

Country Link
FR (1) FR2385099A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0044988A2 (fr) * 1980-07-30 1982-02-03 VEB Arzneimittelwerk Dresden Séparation du ligand libre et du ligand lié aux anticorps pour les immuno-essais à ligand marqué
EP0062892A1 (fr) * 1981-04-13 1982-10-20 American Hoechst Corporation Méthode de détermination immunochimique par incubation unique de la créatine phosphokinase MB
EP0078734A1 (fr) * 1981-10-29 1983-05-11 BIOMERIEUX, Société Anonyme dite Procédé de dosage immunologique mettant en oeuvre deux anticorps, le deuxième anticorps étant purifié par chromatographie d'affinité, supports pour sa mise en oeuvre
US4399217A (en) * 1979-05-02 1983-08-16 Laboratoires Goella Process and a device for the determination of serum lipoproteins
EP0120602A2 (fr) * 1983-02-24 1984-10-03 AMERSHAM INTERNATIONAL plc Méthodes d'analyse

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399217A (en) * 1979-05-02 1983-08-16 Laboratoires Goella Process and a device for the determination of serum lipoproteins
EP0044988A2 (fr) * 1980-07-30 1982-02-03 VEB Arzneimittelwerk Dresden Séparation du ligand libre et du ligand lié aux anticorps pour les immuno-essais à ligand marqué
EP0044988A3 (en) * 1980-07-30 1982-05-05 Veb Arzneimittelwerk Dresden Separation of the free and antibody-bound ligand for labelled-ligand immuno-assays
EP0062892A1 (fr) * 1981-04-13 1982-10-20 American Hoechst Corporation Méthode de détermination immunochimique par incubation unique de la créatine phosphokinase MB
EP0078734A1 (fr) * 1981-10-29 1983-05-11 BIOMERIEUX, Société Anonyme dite Procédé de dosage immunologique mettant en oeuvre deux anticorps, le deuxième anticorps étant purifié par chromatographie d'affinité, supports pour sa mise en oeuvre
EP0120602A2 (fr) * 1983-02-24 1984-10-03 AMERSHAM INTERNATIONAL plc Méthodes d'analyse
EP0120602A3 (en) * 1983-02-24 1988-01-13 Amersham International Plc Assay methods

Also Published As

Publication number Publication date
FR2385099B1 (fr) 1982-02-26

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Legal Events

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TP Transmission of property
ST Notification of lapse