FR2345459A2 - Fixation of organic cpds. contg. sugar residues - by oxidn. and coupling to a support contg. amino gps. - Google Patents

Fixation of organic cpds. contg. sugar residues - by oxidn. and coupling to a support contg. amino gps.

Info

Publication number
FR2345459A2
FR2345459A2 FR7608966A FR7608966A FR2345459A2 FR 2345459 A2 FR2345459 A2 FR 2345459A2 FR 7608966 A FR7608966 A FR 7608966A FR 7608966 A FR7608966 A FR 7608966A FR 2345459 A2 FR2345459 A2 FR 2345459A2
Authority
FR
France
Prior art keywords
contg
support
gps
fixation
oxidn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7608966A
Other languages
French (fr)
Other versions
FR2345459B2 (en
Inventor
Gerard Quash
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Institut National de la Sante et de la Recherche Medicale INSERM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Sante et de la Recherche Medicale INSERM filed Critical Institut National de la Sante et de la Recherche Medicale INSERM
Priority to FR7608966A priority Critical patent/FR2345459A2/en
Priority to CA265,244A priority patent/CA1083508A/en
Priority to SE7612482A priority patent/SE7612482L/en
Priority to CH1417576A priority patent/CH622269A5/fr
Priority to GB14138/79A priority patent/GB1571993A/en
Priority to GB47047/76A priority patent/GB1571992A/en
Priority to US05/740,840 priority patent/US4217338A/en
Priority to JP51136824A priority patent/JPS5929817B2/en
Priority to DE19762661112 priority patent/DE2661112C2/en
Priority to DE19762651680 priority patent/DE2651680A1/en
Priority to NLAANVRAGE7612680,A priority patent/NL186656C/en
Publication of FR2345459A2 publication Critical patent/FR2345459A2/en
Priority to US06/192,479 priority patent/US4419444A/en
Application granted granted Critical
Publication of FR2345459B2 publication Critical patent/FR2345459B2/fr
Priority to SE8107631A priority patent/SE8107631L/en
Priority to SE8107632A priority patent/SE8107632L/en
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Chemical fixation of organic substances contg. a sugar residue to a support contg. >=1 reactive NH2 gp. is effected by oxidising >=1 CH2OH gp. in the sugar residue to CHO, and reacting the resulting CHO gps. with NH2 gps. carried by side chains fixed to an insoluble solid support. When the NH2 gp. is a prim. amine function, the resulting Schiff's base is stabilised by redn. Process can be used to immobilise glycoproteins such as antibodies, antigens, hormones and enzymes and also glycolipids and polysaccharides. The immobilised antibodies and antigens are esp. suitable for use in agglutination-type immunoassay tests. The organic substances are fixed in highly stable form and in good yields without loss of their specific activity. In an example, equine immunogammaglobulins were oxidised with aq. NaIO4 and coupled with a support obtd. by reacting carboxylated polystyrene spheres with hexamethylenediamine. The prod. was then reduced with NaBH4.
FR7608966A 1975-11-13 1976-03-26 Fixation of organic cpds. contg. sugar residues - by oxidn. and coupling to a support contg. amino gps. Granted FR2345459A2 (en)

Priority Applications (14)

Application Number Priority Date Filing Date Title
FR7608966A FR2345459A2 (en) 1976-03-26 1976-03-26 Fixation of organic cpds. contg. sugar residues - by oxidn. and coupling to a support contg. amino gps.
CA265,244A CA1083508A (en) 1975-11-13 1976-11-09 Supports having lateral chains, process for obtaining such supports, process for the fixation of organic compounds comprising a glucidic residue on said supports, products and reactives from said chimical fixation
CH1417576A CH622269A5 (en) 1975-11-13 1976-11-10
SE7612482A SE7612482L (en) 1975-11-13 1976-11-10 NEW CARRIERS DISPLAYING SIDE CHAINS, PROCESSES FOR RECEIVING THESE CARRIERS, PROCESS FOR FIXING ORGANIC ASSOCIATIONS WITH CARBOHYDRATE REMAINERS ON THE SAID CARRIERS, PRODUCTS AND REAGENTS RESULTING FROM THE SAID ...
US05/740,840 US4217338A (en) 1975-11-13 1976-11-11 Novel supports carrying side chains, processes for obtaining these supports, process for attaching organic compounds having carbohydrate residues on said supports, products and reagents resulting from said _chemical fixation
GB47047/76A GB1571992A (en) 1975-11-13 1976-11-11 Process for binding organic compounds containing carbohydrate moieties to solid supports
GB14138/79A GB1571993A (en) 1975-11-13 1976-11-11 Process for producing insoluble solid supports
DE19762661112 DE2661112C2 (en) 1975-11-13 1976-11-12 Process for the preparation of an insoluble carrier
DE19762651680 DE2651680A1 (en) 1975-11-13 1976-11-12 NEW CARRIERS WITH SIDE CHAINS, PROCESS FOR THEIR PRODUCTION AND PROCESS FOR FIXING ORGANIC COMPOUNDS WITH A GLUCIDE REMAIN ON THESE CARRIERS AND THE PRODUCTS RESULTING FROM THEM
JP51136824A JPS5929817B2 (en) 1975-11-13 1976-11-12 Biological reagents and their production methods
NLAANVRAGE7612680,A NL186656C (en) 1975-11-13 1976-11-15 METHOD FOR PREPARING BIOLOGICAL REACTIVES AND PROCESS FOR PREPARING SOLID INSOLUBLE CARRIERS.
US06/192,479 US4419444A (en) 1975-11-13 1980-09-30 Novel supports carrying side chains, processes for obtaining these supports, process for attaching organic compounds having carbohydrate residues one said supports, products and reagents resulting from said chemical fixation
SE8107631A SE8107631L (en) 1975-11-13 1981-12-18 PROCEDURE FOR PREPARING A BEARER
SE8107632A SE8107632L (en) 1975-11-13 1981-12-18 PROCEDURE FOR PREPARING A REAGENT

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7608966A FR2345459A2 (en) 1976-03-26 1976-03-26 Fixation of organic cpds. contg. sugar residues - by oxidn. and coupling to a support contg. amino gps.

Publications (2)

Publication Number Publication Date
FR2345459A2 true FR2345459A2 (en) 1977-10-21
FR2345459B2 FR2345459B2 (en) 1980-11-28

Family

ID=9171045

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7608966A Granted FR2345459A2 (en) 1975-11-13 1976-03-26 Fixation of organic cpds. contg. sugar residues - by oxidn. and coupling to a support contg. amino gps.

Country Status (1)

Country Link
FR (1) FR2345459A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2418237A1 (en) * 1978-02-24 1979-09-21 Sanyo Chemical Ind Ltd PROCESS FOR OBTAINING COMPOSITIONS BASED ON GLYCOPROTEIN DERIVATIVES, PRODUCTS RESULTING FROM THIS PROCESS AND THEIR APPLICATIONS
EP0214330A1 (en) * 1985-09-09 1987-03-18 PROGEN Biotechnik GmbH Method for immobilizing substances in synthetic solid phases, and synthetic solid phases for use in immunosorbent assays
EP0411711A2 (en) * 1989-08-03 1991-02-06 Johnson & Johnson Clinical Diagnostics, Inc. Attachment of compounds to polymeric particles using dication ethers and a kit containing same

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2418237A1 (en) * 1978-02-24 1979-09-21 Sanyo Chemical Ind Ltd PROCESS FOR OBTAINING COMPOSITIONS BASED ON GLYCOPROTEIN DERIVATIVES, PRODUCTS RESULTING FROM THIS PROCESS AND THEIR APPLICATIONS
EP0214330A1 (en) * 1985-09-09 1987-03-18 PROGEN Biotechnik GmbH Method for immobilizing substances in synthetic solid phases, and synthetic solid phases for use in immunosorbent assays
EP0411711A2 (en) * 1989-08-03 1991-02-06 Johnson & Johnson Clinical Diagnostics, Inc. Attachment of compounds to polymeric particles using dication ethers and a kit containing same
EP0411711A3 (en) * 1989-08-03 1991-11-27 Eastman Kodak Company Attachment of compounds to polymeric particles using dication ethers and a kit containing same

Also Published As

Publication number Publication date
FR2345459B2 (en) 1980-11-28

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